Wild Chrysanthemum Granules are newly incorporated into the Chinese Pharmacopoeia (2025 Edition). Numerous impurity peaks appear in the chromatogram of test samples, and linarin cannot be readily separated from adjacent impurity peaks, which interferes with quantitative determination. To eliminate interference from impurity peaks while complying with the pharmacopoeia specifications of the 2025 Edition, chromatographic parameters were optimized to achieve effective peak separation. An HPLC method was accordingly established for simultaneous quantification of chlorogenic acid and linarin in Wild Chrysanthemum Granules.
Analyses were implemented on a C18 chromatographic column in accordance with the chromatographic requirements stipulated in the 2025 Edition of Chinese Pharmacopoeia. In repeatability tests of standard working solutions, the RSD values of retention time for chlorogenic acid and linarin were ≤0.033%, and the RSD values of peak area were ≤0.299%. Their respective tailing factors were 1.027 and 0.994, and the theoretical plate number of linarin peak reached 609521, all conforming to the pharmacopoeial criteria. This method can be adopted as a reference for routine quantitative testing of chlorogenic acid and linarin in Wild Chrysanthemum Granules.
Instruments Used
Persee L820 High Performance Liquid Chromatograph equipped with:
L820-P22 Binary High-Pressure Solvent Delivery Pump
L820-A11 Autosampler
L820-C11 Column Oven
L820-D10 UV‑Vis Detector
LCWin-V5.0 HPLC Workstation (Standard Version V5.0)
L820-C304100001 Solvent Tray
