- Method Overview
This method is based on GB 12752-2010 National Food Safety Standard – Food Additive Vitamin B₂(Riboflavin). After dissolving vitamin B₂, it is diluted with sodium acetate solution, and a 1cm quartz cuvette is used for spectral scanning to determine the maximum absorption peak. The absorbance ratio at 375nm to that at 267nm should be 0.31~0.33, and the absorbance ratio at 222nm to that at 267nm should be 0.36~0.39.
- 2. Instruments and Reagents
2.1 Instruments and Equipment
2.1.1 Testing Instruments
Tu1901 UV Spectrophotometer(240nm~465nm)
1cm Quartz Cuvette
2.1.2 Pretreatment Equipment
Analytical Balance(0.001g precision)
2.2 Reagents
2.2.1 Reagents
Glacial acetic acid
Sodium acetate
2.2.2 Prepare Reagents
Sodium acetate solution(14g/L)
Weigh 1.4g of sodium acetate and dilute it to 100mL with deionized water.
- Operational Procedures
3.1 Sample Preparation
3.1.1 Preparation of Test Solution
Weigh 15mg of the powder sample, dissolve it in 5mL of acetic acid and 200mL of water, and dilute to 500mL.
3.2 Sample Testing
1)Testing Conditions
Spectral Bandwidth: 1nm
Scanning Range: 240nm~465nm
Speed: Medium
Interval: 1nm
2)Sample Testing
Scan the laboratory sample solution to identify maximum absorption at wavelengths of 444nm±1nm, 375nm±1nm, and 267nm±1nm, and measure the absorbance (A).
