{"id":4483,"date":"2026-03-16T10:36:00","date_gmt":"2026-03-16T02:36:00","guid":{"rendered":"https:\/\/www.pgeneral.com\/?p=4483"},"modified":"2026-03-16T10:36:00","modified_gmt":"2026-03-16T02:36:00","slug":"determination-of-vitamin-b%e2%82%82ultraviolet-visible-spectrophotometry-2","status":"publish","type":"post","link":"https:\/\/www.pgeneral.com\/vi\/applications\/bio-tech-and-life-sciences\/determination-of-vitamin-b%e2%82%82ultraviolet-visible-spectrophotometry-2","title":{"rendered":"X\u00e1c \u0111\u1ecbnh vitamin B \u2082  (Ph\u1ed5 quang h\u1ecdc si\u00eau t\u00edm c\u00f3 th\u1ec3 nh\u00ecn th\u1ea5y)"},"content":{"rendered":"<ol>\n<li>T\u1ed5ng quan ph\u01b0\u01a1ng ph\u00e1p<\/li>\n<\/ol>\n<p>Ph\u01b0\u01a1ng ph\u00e1p n\u00e0y d\u1ef1a tr\u00ean ti\u00eau chu\u1ea9n an to\u00e0n th\u1ef1c ph\u1ea9m qu\u1ed1c gia GB 12752-2010 &#8211; Ph\u1ee5 gia th\u1ef1c ph\u1ea9m Vitamin B \u2082  (Riboflavin). Sau khi h\u00f2a tan vitamin B \u2082, n\u00f3 \u0111\u01b0\u1ee3c pha lo\u00e3ng v\u1edbi dung d\u1ecbch natri acetat, v\u00e0 m\u1ed9t cuvette th\u1ea1ch anh 1cm \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 qu\u00e9t quang ph\u1ed5 \u0111\u1ec3 x\u00e1c \u0111\u1ecbnh \u0111\u1ec9nh h\u1ea5p th\u1ee5 t\u1ed1i \u0111a. T\u1ef7 l\u1ec7 h\u1ea5p th\u1ee5 \u1edf 375nm v\u1edbi 267nm n\u00ean l\u00e0 0,31 ~ 0,33, v\u00e0 t\u1ef7 l\u1ec7 h\u1ea5p th\u1ee5 \u1edf 222nm v\u1edbi 267nm n\u00ean l\u00e0 0,36 ~ 0,39.<\/p>\n<ol start=\"2\">\n<li>Ph\u1ea1m vi<\/li>\n<\/ol>\n<p>Vitamin B2<\/p>\n<ol start=\"3\">\n<li>C\u00e1c t\u00e0i li\u1ec7u tham kh\u1ea3o<\/li>\n<\/ol>\n<p>GB 14752-2010 * Ti\u00eau chu\u1ea9n an to\u00e0n th\u1ef1c ph\u1ea9m qu\u1ed1c gia &#8211; Ph\u1ee5 gia th\u1ef1c ph\u1ea9m Vitamin B2 (Riboflavin) *<\/p>\n<p>4. D\u1ee5ng c\u1ee5 ki\u1ec3m tra<\/p>\n<p>M\u00e1y quang ph\u1ed5 UV TU1901 (240nm ~ 465nm)<\/p>\n<p>Cuvette Quartz 1cm<\/p>","protected":false},"excerpt":{"rendered":"<p>Method Overview This method is based on GB 12752-2010 National Food Safety Standard &#8211; Food Additive Vitamin B\u2082(Riboflavin). After dissolving vitamin B\u2082, it is diluted with sodium acetate solution, and a 1cm quartz cuvette is used for spectral scanning to determine the maximum absorption peak. The absorbance ratio at 375nm to that at 267nm should [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[77],"tags":[],"class_list":["post-4483","post","type-post","status-publish","format-standard","hentry","category-bio-tech-and-life-sciences"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts\/4483","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/comments?post=4483"}],"version-history":[{"count":0,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts\/4483\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/media?parent=4483"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/categories?post=4483"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/tags?post=4483"}],"curies":[{"name":"WP","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}