{"id":4465,"date":"2026-03-11T14:54:57","date_gmt":"2026-03-11T06:54:57","guid":{"rendered":"https:\/\/www.pgeneral.com\/?p=4465"},"modified":"2026-03-10T14:57:41","modified_gmt":"2026-03-10T06:57:41","slug":"uv-vis-vs-fluorescence-spectrophotometer-choosing-the-right-tool-for-your-lab","status":"publish","type":"post","link":"https:\/\/www.pgeneral.com\/vi\/news\/uv-vis-vs-fluorescence-spectrophotometer-choosing-the-right-tool-for-your-lab\/","title":{"rendered":"UV-Vis vs. Fluorescence Spectrophotometer: Ch\u1ecdn c\u00f4ng c\u1ee5 ph\u00f9 h\u1ee3p cho ph\u00f2ng th\u00ed nghi\u1ec7m c\u1ee7a b\u1ea1n"},"content":{"rendered":"

\"UV-Vis<\/h1>\n

 <\/p>\n

Ph\u1ed5 quang h\u1ecdc l\u00e0 m\u1ed9t k\u1ef9 thu\u1eadt c\u01a1 b\u1ea3n \u0111\u1ec3 \u0111o l\u01b0\u1ee3ng \u00e1nh s\u00e1ng m\u00e0 m\u1ed9t ch\u1ea5t h\u00f3a h\u1ecdc h\u1ea5p th\u1ee5 ho\u1eb7c cho ph\u00e9p th\u00f4ng qua. \u0110i\u1ec1u n\u00e0y x\u1ea3y ra khi m\u1ed9t ch\u00f9m \u00e1nh s\u00e1ng \u0111i qua m\u1ed9t dung d\u1ecbch ch\u1ee9a v\u1eadt li\u1ec7u trong c\u00e2u h\u1ecfi, v\u00e0 sau \u0111\u00f3 ng\u01b0\u1eddi ta \u0111\u00e1nh gi\u00e1 c\u01b0\u1eddng \u0111\u1ed9 c\u1ee7a \u00e1nh s\u00e1ng \u0111i ra ph\u00eda b\u00ean kia. To\u00e0n b\u1ed9 thi\u1ebft l\u1eadp t\u1ea1o ra n\u1ec1n t\u1ea3ng cho m\u00e1y quang ph\u1ed5 UV-Vis. C\u00e1c c\u00f4ng c\u1ee5 n\u00e0y \u0111\u00e1nh gi\u00e1 \u0111\u1ed9 h\u1ea5p th\u1ee5 trong ph\u1ea1m vi tia c\u1ef1c t\u00edm v\u00e0 \u00e1nh s\u00e1ng nh\u00ecn th\u1ea5y. Lu\u1eadt Beer-Lambert ch\u1ec9 ra m\u1ed9t m\u1ed1i li\u00ean h\u1ec7 tr\u1ef1c ti\u1ebfp gi\u1eefa \u0111\u1ed9 h\u1ea5p th\u1ee5, n\u1ed3ng \u0111\u1ed9 v\u00e0 chi\u1ec1u d\u00e0i c\u1ee7a con \u0111\u01b0\u1eddng \u00e1nh s\u00e1ng. K\u1ebft qu\u1ea3 l\u00e0, c\u00e1c nh\u00e0 khoa h\u1ecdc c\u00f3 th\u1ec3 t\u00ecm ra n\u1ed3ng \u0111\u1ed9 ph\u00e2n t\u00edch v\u1edbi \u0111\u1ed9 ch\u00ednh x\u00e1c r\u00f5 r\u00e0ng. M\u1ed9t ng\u01b0\u1eddi t\u00ecm ra n\u1ed3ng \u0111\u1ed9 c\u1ee7a m\u1ed9t ch\u1ea5t ph\u00e2n t\u00edch trong m\u1ed9t dung d\u1ecbch b\u1eb1ng c\u00e1ch nh\u00ecn v\u00e0o t\u00ednh h\u1ea5p th\u1ee5 ho\u1eb7c truy\u1ec1n c\u1ee7a m\u1ed9t v\u1eadt li\u1ec7u. Nh\u1eefng \u0111\u1eb7c \u0111i\u1ec3m n\u00e0y thay \u0111\u1ed5i d\u1ef1a tr\u00ean b\u01b0\u1edbc s\u00f3ng. C\u00e1c c\u00f4ng c\u1ee5 nh\u01b0 v\u1eady \u0111\u01b0\u1ee3c ch\u1ee9ng minh l\u00e0 c\u1ea7n thi\u1ebft cho c\u00f4ng vi\u1ec7c \u0111\u1ecbnh l\u01b0\u1ee3ng trong c\u00e1c l\u0129nh v\u1ef1c nh\u01b0 h\u00f3a h\u1ecdc, sinh h\u1ecdc v\u00e0 khoa h\u1ecdc m\u00f4i tr\u01b0\u1eddng. S\u1ee9c m\u1ea1nh c\u1ee7a ch\u00fang n\u1eb1m trong \u0111\u1ed9 ch\u00ednh x\u00e1c \u1ed5n \u0111\u1ecbnh v\u00e0 k\u1ebft qu\u1ea3 l\u1eb7p l\u1ea1i.<\/p>\n

\u1ee8ng d\u1ee5ng c\u1ee7a m\u00e1y quang ph\u1ed5 UV-Vis<\/b><\/strong><\/h3>\n

M\u00e1y quang ph\u1ed5 UV-Vis \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng r\u1ed9ng r\u00e3i trong ki\u1ec3m tra h\u00f3a h\u1ecdc v\u00e0 sinh h\u00f3a. H\u1ecd gi\u00fap nghi\u00ean c\u1ee9u axit nucleic, protein v\u00e0 c\u00e1c h\u1ee3p ch\u1ea5t h\u1eefu c\u01a1. H\u01a1n n\u1eefa, ch\u00fang gi\u1eef c\u00e1c \u0111i\u1ec3m quan tr\u1ecdng trong ki\u1ec3m tra m\u00f4i tr\u01b0\u1eddng, ch\u1eb3ng h\u1ea1n nh\u01b0 theo d\u00f5i ch\u1ea5t l\u01b0\u1ee3ng n\u01b0\u1edbc ho\u1eb7c ph\u00e1t hi\u1ec7n c\u00e1c ch\u1ea5t \u00f4 nhi\u1ec5m. H\u00e3y xem x\u00e9t T7<\/b><\/u><\/strong>D<\/b><\/u><\/strong>\u00a0M\u00e1y quang ph\u1ed5 UV-Vis<\/b><\/u><\/strong><\/a>cung c\u1ea5p c\u00e1c t\u00ednh n\u0103ng \u0111o l\u01b0\u1eddng quang h\u1ecdc r\u1eafn cho ph\u00e2n t\u00edch DNA \/ protein, ki\u1ec3m tra \u0111\u1ecbnh l\u01b0\u1ee3ng v\u00e0 qu\u00e9t ph\u1ed5. T7 x\u1eed l\u00fd c\u00e1c ph\u00e9p \u0111o quang h\u1ecdc, v\u00e0 n\u00f3 c\u0169ng th\u1ef1c hi\u1ec7n qu\u00e9t ph\u1ed5, x\u00e1c \u0111\u1ecbnh \u0111\u1ecbnh l\u01b0\u1ee3ng v\u00e0 ph\u00e2n t\u00edch DNA \/ Protein. V\u1edbi m\u1ee9c \u0111\u1ed9 t\u1ef1 \u0111\u1ed9ng h\u00f3a m\u1ea1nh m\u1ebd, n\u00f3 cho ph\u00e9p ch\u1ea1y nhanh ch\u00f3ng. \u0110i\u1ec1u n\u00e0y \u0111\u00f2i h\u1ecfi \u00edt c\u00f4ng vi\u1ec7c th\u1ef1c t\u1ebf. Nh\u1eefng ph\u1ea9m ch\u1ea5t nh\u01b0 v\u1eady l\u00e0m cho n\u00f3 ho\u00e0n h\u1ea3o cho c\u00e1c ph\u00f2ng th\u00ed nghi\u1ec7m c\u1ea7n c\u1ea3 c\u00f4ng vi\u1ec7c ch\u00ednh x\u00e1c v\u00e0 c\u00e1c quy tr\u00ecnh tr\u01a1n tru.<\/p>\n

T\u00ednh n\u0103ng ch\u00ednh c\u1ee7a quang ph\u1ed5 quang quang<\/b><\/strong><\/h2>\n

Nhi\u1ec1u lo\u1ea1i nguy\u00ean t\u1eed v\u00e0 ph\u00e2n t\u1eed ph\u00e1t ra hu\u1ef3nh quang. N\u00f3i c\u00e1ch kh\u00e1c, ch\u00fang l\u1ea5y n\u0103ng l\u01b0\u1ee3ng t\u1eeb ph\u1ed5 UV c\u00f3 th\u1ec3 nh\u00ecn th\u1ea5y. Sau \u0111\u00f3, ch\u00fang nhanh ch\u00f3ng gi\u1ea3i ph\u00f3ng ph\u1ea7n l\u1edbn n\u0103ng l\u01b0\u1ee3ng \u0111\u00f3. ph\u1ea7n c\u00f2n l\u1ea1i bi\u1ebfn th\u00e0nh nhi\u1ec7t ho\u1eb7c n\u0103ng l\u01b0\u1ee3ng rung trong m\u00f4i tr\u01b0\u1eddng xung quanh. \u00c1nh s\u00e1ng xu\u1ea5t hi\u1ec7n \u1edf c\u00e1c b\u01b0\u1edbc s\u00f3ng d\u00e0i h\u01a1n so v\u1edbi c\u00e1c b\u01b0\u1edbc s\u00f3ng t\u1eeb ngu\u1ed3n k\u00edch th\u00edch. C\u00e1c chuy\u00ean gia g\u1ecdi \u0111\u00e2y l\u00e0 s\u1ef1 thay \u0111\u1ed5i Stokes, l\u00e0 \u00fd t\u01b0\u1edfng c\u1ed1t l\u00f5i \u0111\u1eb1ng sau s\u1ef1 ph\u00e1t hi\u1ec7n hu\u1ef3nh quang. Hu\u1ef3nh quang ki\u1ec3m tra \u00e1nh s\u00e1ng ph\u00e1t ra, kh\u00f4ng ph\u1ea3i \u00e1nh s\u00e1ng \u0111\u01b0\u1ee3c h\u1ea5p th\u1ee5. V\u00ec l\u00fd do \u0111\u00f3, n\u00f3 cung c\u1ea5p \u0111\u1ed9 nh\u1ea1y t\u1ed1t h\u01a1n nhi\u1ec1u so v\u1edbi c\u00e1c ph\u01b0\u01a1ng ph\u00e1p h\u1ea5p th\u1ee5 c\u0169. Ch\u1ea5t l\u01b0\u1ee3ng n\u00e0y l\u00e0m cho n\u00f3 \u0111\u1eb7c bi\u1ec7t t\u1ed1t \u0111\u1ec3 t\u00ecm d\u1ea5u v\u1ebft. \u1ede \u0111\u00e2y, m\u1ee9c ph\u00e2n t\u00edch ng\u1ed3i \u1edf s\u1ed1 l\u01b0\u1ee3ng r\u1ea5t nh\u1ecf.<\/p>\n

\u1ee8ng d\u1ee5ng c\u1ee7a quang ph\u1ed5 quang quang<\/b><\/strong><\/h3>\n

C\u00e1c c\u00f4ng c\u1ee5 hu\u1ef3nh quang \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng nhi\u1ec1u trong khoa h\u1ecdc sinh ho\u1ea1t, v\u00e0 ch\u00fang h\u1ed7 tr\u1ee3 trong vi\u1ec7c ki\u1ec3m tra c\u00e1c li\u00ean k\u1ebft ph\u00e2n t\u1eed sinh h\u1ecdc v\u00e0 t\u1ebf b\u00e0o ’ ho\u1ea1t \u0111\u1ed9ng b\u00ean trong. C\u00e1c thi\u1ebft b\u1ecb n\u00e0y cho ph\u00e9p \u0111\u1ebfm s\u1eafc n\u00e9t axit nucleic ho\u1eb7c protein, ho\u1ea1t \u0111\u1ed9ng ngay c\u1ea3 \u1edf l\u01b0\u1ee3ng nanomolar. M\u1ed9t \u0111i\u1ec3m h\u1ee3p ch\u1ea5t hu\u1ef3nh quang th\u00f4ng qua c\u00e1c m\u00f4 h\u00ecnh hu\u1ef3nh quang \u0111\u1eb7c bi\u1ec7t c\u1ee7a ch\u00fang. Trong m\u1ed9t s\u1ed1 tr\u01b0\u1eddng h\u1ee3p, m\u1ed9t ch\u1ea5t kh\u00f4ng hu\u1ef3nh quang \u0111\u01b0\u1ee3c \u0111\u00e1nh d\u1ea5u b\u1eb1ng thu\u1ed1c nhu\u1ed9m hu\u1ef3nh quang ho\u1eb7c fluorophore, cho ph\u00e9p ch\u1ea5t kh\u00f4ng hu\u1ef3nh quang xu\u1ea5t hi\u1ec7n tr\u00ean thi\u1ebft b\u1ecb hu\u1ef3nh quang. H\u01a1n n\u1eefa, c\u00e1c thi\u1ebft l\u1eadp n\u00e0y gi\u00fap ch\u1ea9n \u0111o\u00e1n y t\u1ebf v\u00e0 nghi\u00ean c\u1ee9u d\u01b0\u1ee3c ph\u1ea9m. H\u1ecd cho ph\u00e9p th\u1ef1c hi\u1ec7n c\u00e1c th\u1eed nghi\u1ec7m m\u1ea1nh m\u1ebd. V\u00ed d\u1ee5 bao g\u1ed3m \u0111\u1ed9ng h\u1ecdc enzyme ho\u1eb7c ki\u1ec3m tra li\u00ean k\u1ebft thu\u1ed1c. T\u1ea5t c\u1ea3 ph\u1ee5 thu\u1ed9c v\u00e0o \u0111\u1ed9 r\u00f5 r\u00e0ng quang h\u1ecdc t\u1ed1t.<\/p>\n

So s\u00e1nh m\u00e1y quang ph\u1ed5 UV-Vis v\u00e0 hu\u1ef3nh quang<\/b><\/strong><\/h2>\n

Ph\u1ed5 quang h\u1ecdc hu\u1ef3nh quang mang l\u1ea1i \u0111\u1ed9 nh\u1ea1y c\u1ea3m l\u1edbn h\u01a1n. N\u00f3 thu th\u1eadp c\u00e1c photon ph\u00e1t ra \u0111\u01b0\u1ee3c thi\u1ebft l\u1eadp ch\u1ed1ng l\u1ea1i m\u1ed9t thi\u1ebft l\u1eadp t\u1ed1i t\u0103m, kh\u00e1c v\u1edbi vi\u1ec7c \u0111o \u00e1nh s\u00e1ng \u0111i qua m\u1ed9t thi\u1ebft l\u1eadp h\u1ea5p th\u1ee5. Tuy nhi\u00ean, gi\u1edbi h\u1ea1n ph\u00e1t hi\u1ec7n thay \u0111\u1ed5i theo lo\u1ea1i m\u1eabu, thi\u1ebft l\u1eadp thi\u1ebft b\u1ecb v\u00e0 k\u1ebf ho\u1ea1ch quang h\u1ecdc. M\u00e1y quang ph\u1ed5 c\u00f3 th\u1ec3 \u0111\u1ed1i ph\u00f3 v\u1edbi \u00e1nh s\u00e1ng nh\u00ecn th\u1ea5y (tr\u1eafng) ho\u1eb7c \u00e1nh s\u00e1ng c\u1ef1c t\u00edm. Ch\u00fang gi\u1ea3m xu\u1ed1ng b\u01b0\u1edbc s\u00f3ng kho\u1ea3ng 190 nm. Ph\u1ea1m vi n\u00e0y trong ph\u1ed5 gi\u1eef cho c\u1ea3 hai c\u00e1ch ti\u1ebfp c\u1eadn l\u00e0 \u0111\u1ed3ng minh h\u1eefu \u00edch, v\u00e0 ch\u00fang kh\u00f4ng chi\u1ebfn \u0111\u1ea5u v\u1edbi nhau trong c\u00e1c d\u00f2ng ph\u00e2n t\u00edch.<\/p>\n

Y\u00eau c\u1ea7u m\u1eabu v\u00e0 chu\u1ea9n b\u1ecb<\/b><\/strong><\/h3>\n

C\u00e1c thi\u1ebft b\u1ecb UV-Vis th\u01b0\u1eddng y\u00eau c\u1ea7u chu\u1ea9n b\u1ecb m\u1eabu \u0111\u01a1n gi\u1ea3n, c\u00f3 ngh\u0129a l\u00e0 c\u00e1c gi\u1ea3i ph\u00e1p r\u00f5 r\u00e0ng kh\u00f4ng c\u00f3 m\u00e2y. M\u1eb7t kh\u00e1c, hu\u1ef3nh quang th\u01b0\u1eddng c\u1ea7n thi\u1ebft l\u1eadp thu\u1ed1c nhu\u1ed9m ho\u1eb7c th\u1ebb \u0111\u1ec3 k\u00edch ho\u1ea1t c\u00e1c t\u00edn hi\u1ec7u ph\u00e1t ra m\u00e0 m\u1ed9t ng\u01b0\u1eddi c\u00f3 th\u1ec3 \u0111o l\u01b0\u1eddng. V\u00ed d\u1ee5, trong vi\u1ec7c ki\u1ec3m tra c\u00e1c h\u1ee3p ch\u1ea5t kh\u00f4ng hu\u1ef3nh quang nh\u01b0 c\u00e1c ph\u00e2n t\u1eed h\u1eefu c\u01a1 nh\u1ecf ho\u1eb7c ion v\u00f4 h\u1eefu c\u01a1, vi\u1ec7c th\u00eam d\u1ea5u hu\u1ef3nh quang c\u00f3 th\u1ec3 ch\u1ee9ng minh l\u00e0 ch\u00eca kh\u00f3a. Ch\u1ec9 sau \u0111\u00f3, vi\u1ec7c \u0111o l\u01b0\u1eddng m\u1edbi c\u00f3 th\u1ec3 ti\u1ebfn b\u1ed9 t\u1ed1t.<\/p>\n

Y\u1ebfu t\u1ed1 c\u1ea7n xem x\u00e9t khi l\u1ef1a ch\u1ecdn gi\u1eefa m\u00e1y quang ph\u1ed5 UV-Vis v\u00e0 hu\u1ef3nh quang<\/b><\/strong><\/h2>\n

Vi\u1ec7c l\u1ef1a ch\u1ecdn gi\u1eefa hai thi\u1ebft b\u1ecb n\u00e0y ch\u1ee7 y\u1ebfu ph\u1ee5 thu\u1ed9c v\u00e0o m\u1ee5c ti\u00eau ph\u00e2n t\u00edch. UV-Vis n\u1ed5i b\u1eadt trong vi\u1ec7c x\u00e1c \u0111\u1ecbnh n\u1ed3ng \u0111\u1ed9 \u0111\u1ecbnh l\u01b0\u1ee3ng th\u00f4ng qua \u0111o \u0111\u1ed9 h\u1ea5p th\u1ee5 th\u1eb3ng. Hu\u1ef3nh quang l\u00e0m t\u1ed1t h\u01a1n trong h\u00ecnh d\u00e1ng ch\u1ea5t l\u01b0\u1ee3ng bao g\u1ed3m c\u00e1c m\u1ed1i li\u00ean h\u1ec7 ph\u00e2n t\u1eed ho\u1eb7c ph\u00e1t hi\u1ec7n d\u1ea5u v\u1ebft. C\u00e1c ph\u00f2ng th\u00ed nghi\u1ec7m \u0111\u01b0\u1ee3c thi\u1ebft l\u1eadp tr\u00ean gi\u00e1m s\u00e1t ch\u1ea5t l\u01b0\u1ee3ng h\u00e0ng ng\u00e0y c\u00f3 th\u1ec3 nghi\u00eang v\u1ec1 ph\u00eda c\u00e1c lo\u1ea1i UV-Vis m\u1ea1nh m\u1ebd. V\u00ed d\u1ee5 bao g\u1ed3m TU500 UV-vis<\/b><\/u><\/strong><\/a>Ng\u01b0\u1ee3c l\u1ea1i, c\u00e1c \u0111i\u1ec3m nghi\u00ean c\u1ee9u xem x\u00e9t c\u00e1c chuy\u1ec3n \u0111\u1ed9ng ph\u00e2n t\u1eed sinh h\u1ecdc nh\u1eadn \u0111\u01b0\u1ee3c nhi\u1ec1u h\u01a1n t\u1eeb c\u00e1c thi\u1ebft l\u1eadp hu\u1ef3nh quang. Ch\u00fang c\u00f3 th\u1ec3 b\u1eaft \u0111\u01b0\u1ee3c c\u00e1c t\u00edn hi\u1ec7u r\u1ea5t m\u1edd nh\u1ea1t.<\/p>\n

Ng\u00e2n s\u00e1ch v\u00e0 hi\u1ec7u qu\u1ea3 chi ph\u00ed<\/b><\/strong><\/h3>\n

M\u00e1y quang ph\u1ed5 UV-Vis c\u00f3 xu h\u01b0\u1edbng mang l\u1ea1i chi ph\u00ed \u0111\u1ea7u ti\u00ean th\u1ea5p h\u01a1n, v\u00e0 ch\u00fang c\u0169ng c\u00f3 nhu c\u1ea7u b\u1ea3o tr\u00ec nh\u1ecf. \u0110i\u1ec1u n\u00e0y b\u1eaft ngu\u1ed3n t\u1eeb quang h\u1ecdc c\u01a1 b\u1ea3n c\u1ee7a h\u1ecd. H\u1ec7 th\u1ed1ng hu\u1ef3nh quang mang gi\u00e1 mua cao h\u01a1n. Tuy nhi\u00ean, ch\u00fang cung c\u1ea5p s\u1ef1 nh\u1ea1y c\u1ea3m v\u00f4 c\u00f9ng \u1edf nh\u1eefng n\u01a1i c\u1ea7n thi\u1ebft. \u0110i\u1ec1u n\u00e0y ch\u1ee9ng minh l\u00e0 h\u1eefu \u00edch khi k\u00edch th\u01b0\u1edbc m\u1eabu gi\u1eef ch\u1eb7t ch\u1ebd ho\u1eb7c thu\u1ed1c th\u1eed l\u1ebb v\u00e0o h\u1ed7n h\u1ee3p. M\u1ed9t m\u00e1y quang ph\u1ed5 \u0111\u01b0\u1ee3c coi l\u00e0 m\u1ed9t c\u00f4ng c\u1ee5 ph\u00f2ng th\u00ed nghi\u1ec7m chi ti\u1ebft. N\u00f3 ph\u00f9 h\u1ee3p v\u1edbi nhi\u1ec1u l\u0129nh v\u1ef1c khoa h\u1ecdc. Do \u0111\u00f3, thi\u1ebft l\u1eadp ng\u00e2n s\u00e1ch n\u00ean ph\u00f9 h\u1ee3p v\u1edbi c\u1ea3 mong mu\u1ed1n ph\u00e2n t\u00edch hi\u1ec7n t\u1ea1i v\u00e0 t\u01b0\u01a1ng lai ch\u1ea1y tr\u01a1n tru.<\/p>\n

\"T8DCS<\/p>\n

Gi\u1edbi thi\u1ec7u v\u1ec1 PERSEE nh\u01b0 m\u1ed9t nh\u00e0 s\u1ea3n xu\u1ea5t<\/b><\/strong><\/h2>\n

B\u1eaft \u0111\u1ea7u v\u00e0o n\u0103m 1991, PERSEE<\/b><\/u><\/strong><\/a>\u00a0\u0111\u00e3 ph\u00e1t tri\u1ec3n th\u00e0nh m\u1ed9t trong nh\u1eefng nh\u00e0 s\u1ea3n xu\u1ea5t c\u00f4ng c\u1ee5 ph\u00e2n t\u00edch h\u00e0ng \u0111\u1ea7u c\u1ee7a Trung Qu\u1ed1c. N\u00f3 pha tr\u1ed9n nghi\u00ean c\u1ee9u v\u00e0 ph\u00e1t tri\u1ec3n, s\u1ea3n xu\u1ea5t v\u00e0 c\u00e1c d\u00f2ng lan r\u1ed9ng tr\u00ean to\u00e0n th\u1ebf gi\u1edbi. C\u00f4ng ty c\u00f3 ph\u00ea duy\u1ec7t ch\u1ea5t l\u01b0\u1ee3ng ISO9001, v\u00e0 n\u00f3 c\u0169ng gi\u1eef huy hi\u1ec7u x\u1eed l\u00fd m\u00f4i tr\u01b0\u1eddng ISO14001. \u0110i\u1ec1u n\u00e0y \u0111\u1ea3m b\u1ea3o s\u1ef1 tin t\u01b0\u1edfng \u1ed5n \u0111\u1ecbnh v\u00e0o c\u00e1c b\u1ed9 s\u1ea3n ph\u1ea9m c\u1ee7a n\u00f3. Beijing Purkinje General Instrument Co., Ltd. ho\u1ea1t \u0111\u1ed9ng nh\u01b0 m\u1ed9t trang ph\u1ee5c c\u00f4ng ngh\u1ec7 cao m\u1edbi \u0111\u01b0\u1ee3c th\u00e0nh l\u1eadp v\u00e0o n\u0103m 1991. N\u00f3 t\u1eadp trung v\u00e0o nghi\u00ean c\u1ee9u v\u00e0 ph\u00e1t tri\u1ec3n thi\u1ebft b\u1ecb khoa h\u1ecdc, s\u1ea3n xu\u1ea5t v\u00e0 b\u00e1n h\u00e0ng. H\u01a1n 30% nh\u00f3m c\u1ee7a h\u1ecd tham gia tr\u1ef1c ti\u1ebfp v\u00e0o c\u00e1c c\u00f4ng vi\u1ec7c nghi\u00ean c\u1ee9u. Nh\u1eefng m\u1ee5c ti\u00eau n\u00e0y nh\u1eb1m t\u0103ng c\u01b0\u1eddng c\u00e1c c\u00f4ng ngh\u1ec7 quang h\u1ecdc nh\u01b0 c\u00e1c \u0111i\u1ec1u ch\u1ec9nh thi\u1ebft k\u1ebf ch\u00f9m k\u00e9p v\u00e0 h\u1ec7 th\u1ed1ng hi\u1ec7u chu\u1ea9n t\u1ef1 \u0111\u1ed9ng.<\/p>\n

Quan \u0111i\u1ec3m c\u1ee7a PERSEE nh\u1ea5n m\u1ea1nh suy ngh\u0129 m\u1edbi m\u1ebb li\u00ean quan \u0111\u1ebfn l\u1ee3i \u00edch x\u00e3 h\u1ed9i. Nh\u01b0 n\u00f3 n\u00f3i, \u201cKhoa h\u1ecdc v\u00e0 c\u00f4ng ngh\u1ec7 \u0111\u01b0\u1ee3c th\u00fac \u0111\u1ea9y b\u1edfi c\u00e1 nh\u00e2n v\u00e0 nh\u1eb1m mang l\u1ea1i l\u1ee3i \u00edch cho x\u00e3 h\u1ed9i.\u201d C\u00e1c c\u00f4ng c\u1ee5 c\u1ee7a n\u00f3, t\u1eeb m\u00e1y quang ph\u1ed5 ph\u00e2n t\u1eed \u0111\u1ebfn h\u1ec7 th\u1ed1ng nhi\u1ec5m s\u1eafc h\u1ecdc, ki\u1ebfm \u0111\u01b0\u1ee3c s\u1ef1 ch\u00fa \u00fd to\u00e0n c\u1ea7u. H\u1ecd t\u1ecfa s\u00e1ng cho x\u00e2y d\u1ef1ng c\u1ea9n th\u1eadn v\u00e0 s\u1ee9c m\u1ea1nh l\u00e2u d\u00e0i tr\u00ean c\u00e1c l\u0129nh v\u1ef1c kh\u00e1c nhau. Ch\u00fang bao g\u1ed3m d\u01b0\u1ee3c ph\u1ea9m, ki\u1ec3m tra an to\u00e0n th\u1ef1c ph\u1ea9m, h\u00f3a d\u1ea7u, gi\u00e1o d\u1ee5c v\u00e0 b\u1ea3o v\u1ec7 m\u00f4i tr\u01b0\u1eddng.<\/p>\n

Ph\u1ea1m vi s\u1ea3n ph\u1ea9m<\/b><\/strong><\/h3>\n

D\u00f2ng s\u1ea3n ph\u1ea9m c\u1ee7a c\u00f4ng ty bao g\u1ed3m m\u1ecdi th\u1ee9 t\u1eeb c\u00e1c m\u00f4 h\u00ecnh c\u01a1 b\u1ea3n tuy\u1ec7t v\u1eddi cho ph\u00f2ng th\u00ed nghi\u1ec7m gi\u1ea3ng d\u1ea1y \u0111\u1ebfn c\u00e1c k\u1ebf ho\u1ea1ch ch\u00f9m k\u00e9p cao c\u1ea5p ph\u00f9 h\u1ee3p v\u1edbi th\u1eed nghi\u1ec7m d\u1ef1a tr\u00ean quy t\u1eafc. C\u00e1c lo\u1ea1i n\u1ed5i b\u1eadt bao g\u1ed3m d\u00f2ng T7D \/ T7DS. Ch\u00fang s\u1eed d\u1ee5ng l\u01b0\u1edbi holographic \u0111\u1ec3 c\u1eaft \u00e1nh s\u00e1ng l\u1ea1c l\u00f9ng. \u0110\u1ed3ng th\u1eddi, h\u1ecd gi\u1eef s\u1ee9c m\u1ea1nh qu\u00e9t nhanh. C\u00e1c T8DCS<\/b><\/u><\/strong><\/a>\u00a0cung c\u1ea5p b\u0103ng th\u00f4ng c\u00f3 th\u1ec3 \u0111i\u1ec1u ch\u1ec9nh t\u1eeb 0,1 \u0111\u1ebfn 5 nm. T9DCS cho th\u1ea5y \u00e1nh s\u00e1ng l\u1ea1c l\u00f9ng r\u1ea5t th\u1ea5p (\u22640,00004% T NaI \u1edf 220 nm). T\u1ea5t c\u1ea3 \u0111\u1ec1u nh\u1eb1m m\u1ee5c \u0111\u00edch \u0111\u1ea1t c\u00e1c \u0111i\u1ec3m ph\u00e2n t\u00edch kh\u00f3 kh\u0103n \u0111\u01b0\u1ee3c thi\u1ebft l\u1eadp b\u1edfi c\u00e1c ph\u00f2ng th\u00ed nghi\u1ec7m ng\u00e0y nay.<\/p>\n

Ph\u1ea7n k\u1ebft lu\u1eadn<\/b><\/strong><\/h2>\n

UV-Vis v\u00e0 quang ph\u1ed5 quang quang l\u1ea5p \u0111\u1ea7y c\u00e1c vai tr\u00f2 ri\u00eang bi\u1ec7t nh\u01b0ng ph\u00f9 h\u1ee3p trong khoa h\u1ecdc ph\u00e2n t\u00edch. UV-Vis mang l\u1ea1i s\u1ef1 d\u1ec5 d\u00e0ng v\u00e0 b\u1ec1n b\u1ec1n cho c\u00e1c c\u00f4ng vi\u1ec7c \u0111\u1ebfm th\u01b0\u1eddng xuy\u00ean d\u1ef1a tr\u00ean \u00fd t\u01b0\u1edfng Beer-Lambert. Fluorescence cung c\u1ea5p ch\u00eca kh\u00f3a \u0111\u1ed9 nh\u1ea1y cao nh\u1ea5t cho c\u00e1c th\u0103m d\u00f2 sinh h\u1ecdc kh\u00f3 kh\u0103n ho\u1eb7c c\u00f4ng vi\u1ec7c t\u00ecm d\u1ea5u v\u1ebft. L\u1ef1a ch\u1ecdn t\u1ed1t nh\u1ea5t ph\u1ee5 thu\u1ed9c v\u00e0o m\u1ee5c ti\u00eau ph\u00f2ng th\u00ed nghi\u1ec7m. Nh\u1eefng \u0111i\u1ec1u n\u00e0y c\u00f3 th\u1ec3 nh\u1ea5n m\u1ea1nh s\u1ef1 t\u1eadp trung, s\u1ef1 \u0111\u00fang \u0111\u1eafn ho\u1eb7c chi ti\u1ebft ph\u00e2n t\u1eed. Ng\u00e2n s\u00e1ch l\u00e0m vi\u1ec7c c\u0169ng \u0111\u00f3ng m\u1ed9t vai tr\u00f2.<\/p>\n

C\u00e2u h\u1ecfi th\u01b0\u1eddng g\u1eb7p<\/b><\/strong><\/h2>\n

Q1: S\u1ef1 kh\u00e1c bi\u1ec7t ch\u00ednh gi\u1eefa m\u00e1y quang ph\u1ed5 UV-Vis v\u00e0 quang hu\u1ef3nh quang l\u00e0 g\u00ec?<\/b><\/strong>
\n<\/b><\/strong>A1: UV-Vis \u0111o \u0111\u1ed9 h\u1ea5p th\u1ee5 tr\u1ef1c ti\u1ebfp t\u1eeb \u00e1nh s\u00e1ng truy\u1ec1n theo c\u00e1c nguy\u00ean t\u1eafc \u0111\u1ecbnh lu\u1eadt Beer-Lambert; hu\u1ef3nh quang ph\u00e1t hi\u1ec7n \u00e1nh s\u00e1ng ph\u00e1t ra sau khi k\u00edch th\u00edch \u1edf b\u01b0\u1edbc s\u00f3ng c\u1ee5 th\u1ec3 d\u1ef1a tr\u00ean hi\u1ec7n t\u01b0\u1ee3ng chuy\u1ec3n \u0111\u1ed5i Stokes.<\/p>\n

 <\/p>\n

Q2: M\u1ed9t c\u00f4ng c\u1ee5 c\u00f3 th\u1ec3 thay th\u1ebf c\u00f4ng c\u1ee5 kh\u00e1c kh\u00f4ng?<\/b><\/strong>
\n<\/b><\/strong>A2: Kh\u00f4ng; m\u1ed7i m\u1ee5c \u0111\u00edch ph\u00e2n t\u00edch kh\u00e1c nhau - UV-Vis xu\u1ea5t s\u1eafc trong ph\u00e2n t\u00edch n\u1ed3ng \u0111\u1ed9 \u0111\u1ecbnh l\u01b0\u1ee3ng trong khi hu\u1ef3nh quang chuy\u00ean ph\u00e1t hi\u1ec7n si\u00eau nh\u1ea1y c\u1ea3m li\u00ean quan \u0111\u1ebfn c\u00e1c ph\u00e2n t\u1eed hu\u1ef3nh quang.<\/p>\n

 <\/p>\n

Q3: L\u00e0m th\u1ebf n\u00e0o \u0111\u1ec3 t\u00f4i quy\u1ebft \u0111\u1ecbnh quang ph\u1ed5 m\u00e1y ph\u00f9 h\u1ee3p v\u1edbi ph\u00f2ng th\u00ed nghi\u1ec7m c\u1ee7a t\u00f4i?<\/b><\/strong>
\n<\/b><\/strong>A3: \u0110\u00e1nh gi\u00e1 c\u00e1c m\u1ee5c ti\u00eau ph\u00e2n t\u00edch ch\u00ednh c\u1ee7a b\u1ea1n (\u0111\u1ecbnh l\u01b0\u1ee3ng so v\u1edbi ch\u1ea5t l\u01b0\u1ee3ng), xem x\u00e9t c\u00e1c lo\u1ea1i m\u1eabu \u0111\u01b0\u1ee3c x\u1eed l\u00fd th\u01b0\u1eddng xuy\u00ean, xem x\u00e9t ph\u00e2n b\u1ed5 ng\u00e2n s\u00e1ch c\u00f3 s\u1eb5n, bao g\u1ed3m chi ph\u00ed b\u1ea3o tr\u00ec, v\u00e0 \u0111i\u1ec1u ch\u1ec9nh c\u00e1c y\u1ebfu t\u1ed1 n\u00e0y v\u1edbi c\u00e1c m\u1ee5c ti\u00eau nghi\u00ean c\u1ee9u d\u00e0i h\u1ea1n tr\u01b0\u1edbc khi l\u1ef1a ch\u1ecdn.<\/p>\n

 <\/p>","protected":false},"excerpt":{"rendered":"

  Spectrophotometry serves as a basic technique for gauging the amount of light that a chemical substance absorbs or lets through. This happens when a light beam travels through a solution containing the material in question, and then one assesses the strength of the light coming out the other side. The whole setup creates the […]<\/p>\n","protected":false},"author":1,"featured_media":4461,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[38,37],"tags":[],"class_list":["post-4465","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-industry-information","category-news"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts\/4465","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/comments?post=4465"}],"version-history":[{"count":2,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts\/4465\/revisions"}],"predecessor-version":[{"id":4467,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts\/4465\/revisions\/4467"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/media\/4461"}],"wp:attachment":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/media?parent=4465"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/categories?post=4465"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/tags?post=4465"}],"curies":[{"name":"WP","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}