![\"Ph\u00e2n](\"https:\/\/www.pgeneral.com\/wp-content\/uploads\/2026\/01\/High-Resolution-Mass-Spectrometry-Defining-Resolution-in-Analytical-Terms.webp\")
<\/div>\nTrong quang ph\u1ed5 kh\u1ed1i l\u01b0\u1ee3ng \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao (HRMS), \u0111\u1ed9 ph\u00e2n gi\u1ea3i c\u00f3 ngh\u0129a l\u00e0 thi\u1ebft b\u1ecb’ K\u1ef9 n\u0103ng ph\u00e2n bi\u1ec7t c\u00e1c ion v\u1edbi g\u1ea7n nh\u01b0 c\u00f9ng m\u1ed9t gi\u00e1 tr\u1ecb kh\u1ed1i l\u01b0\u1ee3ng \u0111\u1ec3 s\u1ea1c (m \/ z). K\u1ef9 n\u0103ng n\u00e0y r\u1ea5t quan tr\u1ecdng \u0111\u1ec3 ph\u00e1t hi\u1ec7n c\u00e1c h\u1ee3p ch\u1ea5t m\u1ed9t c\u00e1ch ch\u00ednh x\u00e1c, \u0111\u1eb7c bi\u1ec7t l\u00e0 trong c\u00e1c h\u1ed7n h\u1ee3p kh\u00f3 kh\u0103n. Theo thu\u1eadt ng\u1eef to\u00e1n h\u1ecdc, \u0111\u1ed9 ph\u00e2n gi\u1ea3i \u0111\u01b0\u1ee3c hi\u1ec3n th\u1ecb nh\u01b0 R = m \/ \u0394m. \u1ede \u0111\u00e2y, “ m” vi\u1ebft t\u1eaft c\u1ee7a m \/ z \u1edf \u0111\u1ec9nh ch\u00ednh, v\u00e0 “ \u0110m” c\u00f3 ngh\u0129a l\u00e0 \u0111\u1ec9nh’ Chi\u1ec1u r\u1ed9ng s \u1edf chi\u1ec1u r\u1ed9ng \u0111\u1ea7y \u0111\u1ee7 \u1edf m\u1ed9t n\u1eeda t\u1ed1i \u0111a (FWHM). C\u00e1ch \u0111\u1ecbnh ngh\u0129a n\u00e0y cung c\u1ea5p m\u1ed9t c\u00e1ch ti\u00eau chu\u1ea9n \u0111\u1ec3 ki\u1ec3m tra hi\u1ec7u su\u1ea5t c\u1ee7a c\u00e1c thi\u1ebft b\u1ecb kh\u00e1c nhau.<\/p>\n
M\u1ecdi ng\u01b0\u1eddi t\u00ednh to\u00e1n \u0111\u1ed9 ph\u00e2n gi\u1ea3i b\u1eb1ng c\u00e1ch xem c\u00e1ch hai \u0111\u1ec9nh t\u00e1ch nhau d\u1ef1a tr\u00ean chi\u1ec1u r\u1ed9ng trung b\u00ecnh c\u1ee7a ch\u00fang \u1edf c\u01a1 s\u1edf (tR2 > tR1). \u0110\u1ed9 ph\u00e2n gi\u1ea3i m\u1ea1nh m\u1ebd cho ph\u00e9p ki\u1ec3m tra kh\u1ed1i l\u01b0\u1ee3ng ch\u00ednh x\u00e1c, gi\u00fap s\u1eafp x\u1ebfp c\u00e1c h\u1ee3p ch\u1ea5t v\u1edbi kh\u1ed1i l\u01b0\u1ee3ng danh ngh\u0129a r\u1ea5t g\u1ea7n. V\u00ec v\u1eady, n\u1ebfu kh\u00f4ng c\u00f3 \u0111\u1ed9 ph\u00e2n gi\u1ea3i t\u1ed1t, b\u1ea1n c\u00f3 th\u1ec3 tr\u1ed9n ch\u00fang d\u1ec5 d\u00e0ng.<\/p>\n
C\u00e1c s\u1ed1 li\u1ec7u kh\u00e1c nhau \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 \u0111\u1ecbnh l\u01b0\u1ee3ng \u0111\u1ed9 ph\u00e2n gi\u1ea3i trong HRMS l\u00e0 g\u00ec?<\/strong><\/h3>\nC\u00e1c chuy\u00ean gia \u0111o \u0111\u1ed9 ph\u00e2n gi\u1ea3i theo m\u1ed9t v\u00e0i c\u00e1ch. Ph\u01b0\u01a1ng ph\u00e1p FWHM l\u00e0 ph\u01b0\u01a1ng ph\u00e1p m\u00e0 h\u1ea7u h\u1ebft m\u1ecdi ng\u01b0\u1eddi s\u1eed d\u1ee5ng. N\u00f3 gi\u1eef m\u1ecdi th\u1ee9 \u1ed5n \u0111\u1ecbnh khi ki\u1ec3m tra c\u00e1ch thi\u1ebft b\u1ecb ho\u1ea1t \u0111\u1ed9ng. M\u1ed9t c\u00e1ch ti\u1ebfp c\u1eadn kh\u00e1c, ph\u01b0\u01a1ng ph\u00e1p thung l\u0169ng 10%, ki\u1ec3m tra s\u1ef1 t\u00e1ch \u0111\u1ec9nh t\u1ea1i m\u1ed9t \u0111i\u1ec3m c\u01b0\u1eddng \u0111\u1ed9 th\u1ea5p h\u01a1n. Th\u00eam v\u00e0o \u0111\u00f3, ph\u1ea7n m\u1ec1m sau khi th\u1ef1c t\u1ebf c\u00f3 th\u1ec3 thay \u0111\u1ed5i s\u1ed1 \u0111\u1ed9 ph\u00e2n gi\u1ea3i. C\u00e1c c\u00f4ng c\u1ee5 \u0111\u1ec3 l\u00e0m s\u1ea1ch \u0111\u1ec9nh cao v\u00e0 s\u1eeda ch\u1eefa \u0111\u01b0\u1eddng c\u01a1 b\u1ea3n \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn c\u00e1c con s\u1ed1 n\u00e0y. \u0110i\u1ec1u n\u00e0y x\u1ea3y ra r\u1ea5t nhi\u1ec1u v\u1edbi c\u00e1c \u0111\u1ec9nh ch\u1ed3ng ch\u00e9o ho\u1eb7c khi c\u00e1c t\u00edn hi\u1ec7u y\u1ebfu so v\u1edbi ti\u1ebfng \u1ed3n. K\u1ebft qu\u1ea3 l\u00e0, c\u00e1c b\u00e1o c\u00e1o cu\u1ed1i c\u00f9ng c\u00f3 th\u1ec3 kh\u00e1c nhau d\u1ef1a tr\u00ean vi\u1ec7c x\u1eed l\u00fd.<\/p>\n
Y\u1ebfu t\u1ed1 \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn \u0111\u1ed9 ph\u00e2n gi\u1ea3i quang ph\u1ed5 kh\u1ed1i l\u01b0\u1ee3ng<\/strong><\/h2>\nC\u00e1ch m\u00e1y ph\u00e2n t\u00edch kh\u1ed1i l\u01b0\u1ee3ng \u0111\u01b0\u1ee3c x\u00e2y d\u1ef1ng r\u1ea5t quan tr\u1ecdng. H\u1ea7u h\u1ebft c\u00e1c \u0111\u01a1n v\u1ecb TOFMS, ch\u1eb3ng h\u1ea1n nh\u01b0 t\u1eeb JEOL, cung c\u1ea5p chi ti\u1ebft kh\u1ed1i l\u01b0\u1ee3ng v\u1eefng ch\u1eafc v\u00e0 \u0111\u1ecdc kh\u1ed1i l\u01b0\u1ee3ng ch\u00ednh x\u00e1c. B\u1edfi v\u00ec \u0111i\u1ec1u n\u00e0y, h\u1ecd gi\u00fap t\u00ecm ra trang \u0111i\u1ec3m nguy\u00ean t\u1ed1 th\u00f4ng qua ki\u1ec3m tra kh\u1ed1i l\u01b0\u1ee3ng c\u1ea9n th\u1eadn. B\u00ean c\u1ea1nh \u0111\u00f3, c\u00e1c con \u0111\u01b0\u1eddng ion v\u00e0 c\u00e1ch ion di chuy\u1ec3n \u0111\u1ecbnh h\u00ecnh tr\u1ecdng t\u00e2m tr\u01b0\u1edbc khi ch\u00fang \u0111\u00e2m v\u00e0o m\u00e1y d\u00f2. C\u00e1c b\u1ed9 ph\u1eadn ph\u00e1t hi\u1ec7n v\u00e0 t\u0103ng c\u01b0\u1eddng t\u00edn hi\u1ec7u, nh\u01b0 \u0111i\u1ec7n t\u1eed \u0111\u1ec3 khu\u1ebfch \u0111\u1ea1i v\u00e0 t\u1ea7n su\u1ea5t ch\u00fang l\u1ea5y m\u1eabu, c\u0169ng \u0111\u00f3ng m\u1ed9t vai tr\u00f2. Ch\u00fang gi\u1eef cho th\u00f4ng tin ion r\u00f5 r\u00e0ng, h\u1ed7 tr\u1ee3 \u0111\u1ed9 ph\u00e2n gi\u1ea3i t\u1ed5ng th\u1ec3 t\u1ed1t h\u01a1n.<\/p>\n
\u0110i\u1ec1u ki\u1ec7n ho\u1ea1t \u0111\u1ed9ng thay \u0111\u1ed5i \u0111\u1ed9 ph\u00e2n gi\u1ea3i thi\u1ebft b\u1ecb nh\u01b0 th\u1ebf n\u00e0o?<\/strong><\/h3>\nC\u00e0i \u0111\u1eb7t trong ngu\u1ed3n ion, bao g\u1ed3m nhi\u1ec7t, c\u01b0\u1eddng \u0111\u1ed9 electron v\u00e0 \u00e1p su\u1ea5t kh\u00f4ng kh\u00ed, thay \u0111\u1ed5i c\u00e1ch ion h\u00ecnh th\u00e0nh v\u00e0 lan r\u1ed9ng. Nhi\u1ec7t \u0111\u1ed9 \u1ea5m h\u01a1n v\u00e0 \u0111i\u1ec7n \u00e1p \u0111\u01b0\u1ee3c \u0111i\u1ec1u ch\u1ec9nh tinh t\u1ebf l\u00e0m cho ion h\u00f3a \u0111\u1ed3ng \u0111\u1ec1u h\u01a1n. Tuy nhi\u00ean, b\u1ea1n c\u1ea7n theo d\u00f5i ch\u00fang ch\u1eb7t ch\u1ebd \u0111\u1ec3 ng\u0103n ch\u1eb7n s\u1ef1 ph\u00e1 v\u1ee1 kh\u00f4ng mong mu\u1ed1n trong c\u00e1c ph\u00e2n t\u1eed ho\u1eb7c t\u00edn hi\u1ec7u y\u1ebfu h\u01a1n.<\/p>\n
M\u1ed9t \u0111i\u1ec3m quan tr\u1ecdng kh\u00e1c l\u00e0 thi\u1ebft b\u1ecb qu\u00e9t nhanh nh\u01b0 th\u1ebf n\u00e0o. Qu\u00e9t nhanh gi\u1ea3m th\u1eddi gian \u0111\u1ec3 l\u1ea5y chi ti\u1ebft t\u00edn hi\u1ec7u, c\u00f3 th\u1ec3 gi\u1ea3m \u0111\u1ed9 ph\u00e2n gi\u1ea3i. \u0110\u1ed3ng th\u1eddi, nh\u1eefng th\u1ee9 trong m\u1eabu, nh\u01b0 ma tr\u1eadn, c\u00f3 th\u1ec3 ch\u1eb7n c\u00e1c ion. \u0110i\u1ec1u n\u00e0y d\u1eabn \u0111\u1ebfn c\u00e1c \u0111\u1ec9nh r\u1ed9ng h\u01a1n ho\u1eb7c cong, c\u1eaft gi\u1ea3m thi\u1ebft b\u1ecb’ s t\u00e1ch \u0111i\u1ec7n. Do \u0111\u00f3, \u0111i\u1ec1u ch\u1ec9nh c\u1ea9n th\u1eadn gi\u00fap duy tr\u00ec hi\u1ec7u su\u1ea5t m\u1ea1nh m\u1ebd.<\/p>\n
So s\u00e1nh gi\u1eefa \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao v\u00e0 \u0111\u1ed9 ph\u00e2n gi\u1ea3i th\u1ea5p<\/strong><\/h2>\nC\u00e1c thi\u1ebft b\u1ecb c\u00f3 \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao mang l\u1ea1i l\u1ee3i \u00edch l\u1edbn trong vi\u1ec7c ch\u1ecdn m\u1ee5c ti\u00eau v\u00e0 x\u00e2y d\u1ef1ng ni\u1ec1m tin v\u00e0o k\u1ebft qu\u1ea3. Ch\u00fang c\u00f3 th\u1ec3 chia kh\u1ed1i l\u01b0\u1ee3ng ngay c\u1ea3 cho c\u00e1c ion c\u00f3 c\u00f9ng m\u1ed9t s\u1ed1 kh\u1ed1i l\u01b0\u1ee3ng c\u01a1 b\u1ea3n. Ngo\u00e0i ra, ch\u00fang cho ph\u00e9p b\u1ea1n l\u00e0m vi\u1ec7c ra trang \u0111i\u1ec3m c\u1ee7a c\u00e1c y\u1ebfu t\u1ed1. \u0110i\u1ec1u n\u00e0y ch\u1ee9ng minh r\u1ea5t h\u1eefu \u00edch trong c\u00e1c m\u1eabu \u0111\u1ea7y \u0111\u1ee7 tr\u00f4ng gi\u1ed1ng nhau ho\u1eb7c khi t\u00ecm ki\u1ebfm m\u00e0 kh\u00f4ng c\u00f3 danh s\u00e1ch c\u00e1c h\u1ee3p ch\u1ea5t \u0111\u01b0\u1ee3c thi\u1ebft l\u1eadp. Ngo\u00e0i ra, c\u00e1c h\u1ec7 th\u1ed1ng n\u00e0y cung c\u1ea5p \u0111\u1ed9 ch\u00ednh x\u00e1c kh\u1ed1i l\u01b0\u1ee3ng t\u1ed1t h\u01a1n. \u0110i\u1ec1u \u0111\u00f3 l\u00e0m t\u0103ng s\u1ef1 ch\u1eafc ch\u1eafn trong vi\u1ec7c li\u00ean k\u1ebft v\u1edbi c\u00e1c c\u00f4ng th\u1ee9c ph\u00e2n t\u1eed. C\u00e1c t\u00ednh n\u0103ng nh\u01b0 v\u1eady r\u1ea5t quan tr\u1ecdng trong c\u00e1c l\u0129nh v\u1ef1c nh\u01b0 theo d\u00f5i m\u00f4i tr\u01b0\u1eddng ho\u1eb7c ki\u1ec3m tra ch\u1ea5t l\u01b0\u1ee3ng thu\u1ed1c. N\u00f3i t\u00f3m l\u1ea1i, \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao t\u0103ng c\u01b0\u1eddng tr\u00f2 ch\u01a1i cho c\u00e1c ph\u00e2n t\u00edch kh\u00f3 kh\u0103n.<\/p>\n
Nh\u1eefng h\u1ea1n ch\u1ebf c\u1ee7a vi\u1ec7c s\u1eed d\u1ee5ng MS \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao l\u00e0 g\u00ec?<\/strong><\/h3>\nNgay c\u1ea3 v\u1edbi \u0111i\u1ec3m m\u1ea1nh c\u1ee7a h\u1ecd, c\u00e1c thi\u1ebft l\u1eadp \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao chi ph\u00ed nhi\u1ec1u h\u01a1n v\u00e0 m\u1ea5t nhi\u1ec1u n\u1ed7 l\u1ef1c \u0111\u1ec3 ch\u1ea1y h\u01a1n nh\u1eefng thi\u1ebft l\u1eadp c\u01a1 b\u1ea3n. H\u1ecd c\u1ea7n c\u00e1c b\u01b0\u1edbc \u0111i\u1ec1u ch\u1ec9nh \u0111\u1eb7c bi\u1ec7t \u0111\u1ec3 ti\u1ebfp t\u1ee5c ho\u1ea1t \u0111\u1ed9ng t\u1ed1t. Ngo\u00e0i ra, h\u1ecd ph\u1ee5 thu\u1ed9c v\u00e0o ph\u1ea7n m\u1ec1m th\u00f4ng minh \u0111\u1ec3 hi\u1ec3u \u00fd ngh\u0129a c\u1ee7a d\u1eef li\u1ec7u. C\u00e1c \u0111\u01a1n v\u1ecb nh\u01b0 Orbitraps ho\u1eb7c FT-ICR y\u00eau c\u1ea7u ki\u1ec3m so\u00e1t ch\u1eb7t ch\u1ebd m\u00f4i tr\u01b0\u1eddng xung quanh v\u00e0 b\u1ea3o tr\u00ec \u1ed5n \u0111\u1ecbnh. \u0110i\u1ec1u g\u00ec’ H\u01a1n n\u1eefa, l\u01b0\u1ee3ng d\u1eef li\u1ec7u kh\u1ed5ng l\u1ed3 t\u1eeb c\u00e1c ch\u1ea1y n\u00e0y \u0111\u00f2i h\u1ecfi c\u00e1c m\u00e1y t\u00ednh m\u1ea1nh m\u1ebd \u0111\u1ec3 x\u1eed l\u00fd t\u1ea5t c\u1ea3. V\u00ec v\u1eady, trong khi m\u1ea1nh m\u1ebd, h\u1ecd \u0111\u00f2i h\u1ecfi nhi\u1ec1u ngu\u1ed3n l\u1ef1c h\u01a1n t\u1ed5ng th\u1ec3.<\/p>\n
C\u00e1c \u1ee9ng d\u1ee5ng y\u00eau c\u1ea7u ph\u1ed5 \u0111o kh\u1ed1i l\u01b0\u1ee3ng \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao<\/strong><\/h2>\n <\/p>\n
![\"nhi\u1ec5m](\"https:\/\/www.pgeneral.com\/wp-content\/uploads\/2026\/01\/gas-chromatography-G5-GC.webp\")
<\/div>\nTrong c\u00e1c m\u00f4i tr\u01b0\u1eddng kh\u00f3 kh\u0103n nh\u01b0 c\u00e1c m\u1eabu m\u00f4i tr\u01b0\u1eddng ho\u1eb7c cu\u1ed9c s\u1ed1ng v\u1edbi nhi\u1ec1u h\u1ee3p ch\u1ea5t tr\u1ed9n v\u1edbi nhau, HRMS n\u1ed5i b\u1eadt \u0111\u1ec3 t\u00ecm nh\u1eefng \u0111i\u1ec1u ch\u01b0a bi\u1ebft m\u00e0 kh\u00f4ng ch\u1ec9 ph\u00f9 h\u1ee3p v\u1edbi danh s\u00e1ch. M\u1ed9t TOFMS chi ti\u1ebft c\u00f3 th\u1ec3 t\u1ef1 ph\u00e1t hi\u1ec7n c\u00e1c m\u1ee5c kh\u00f4ng bi\u1ebft, m\u00e0 kh\u00f4ng c\u1ea7n s\u1ef1 gi\u00fap \u0111\u1ee1 c\u1ee7a th\u01b0 vi\u1ec7n. N\u00f3 ph\u00f9 h\u1ee3p v\u1edbi c\u00e1c t\u00ecm ki\u1ebfm r\u1ed9ng r\u00e3i. C\u00e1c m\u1ea3nh kh\u1ed1i l\u01b0\u1ee3ng ch\u00ednh x\u00e1c v\u00e0 c\u00e1c d\u1ea5u hi\u1ec7u \u0111\u1ed3ng v\u1ecb th\u00eam v\u00e0o vi\u1ec7c t\u00ecm ra c\u1ea5u tr\u00fac. C\u00e1ch ti\u1ebfp c\u1eadn n\u00e0y t\u1ecfa s\u00e1ng b\u1edfi v\u00ec n\u00f3 x\u1eed l\u00fd s\u1ef1 ph\u1ee9c t\u1ea1p tr\u1ef1c ti\u1ebfp. V\u00ed d\u1ee5, trong c\u00e1c m\u1eabu \u0111\u00f4ng \u0111\u00fac, n\u00f3 k\u00e9o ra c\u00e1c chi ti\u1ebft m\u00e0 c\u00e1c c\u00f4ng c\u1ee5 \u0111\u01a1n gi\u1ea3n h\u01a1n b\u1ecf l\u1ee1. Do \u0111\u00f3, n\u00f3 h\u1ed7 tr\u1ee3 c\u00e1i nh\u00ecn s\u00e2u s\u1eafc h\u01a1n v\u1ec1 nh\u1eefng g\u00ec’ S hi\u1ec7n t\u1ea1i.<\/p>\n
HRMS c\u1ea3i thi\u1ec7n quy tr\u00ecnh l\u00e0m vi\u1ec7c \u0111\u1ecbnh l\u01b0\u1ee3ng nh\u01b0 th\u1ebf n\u00e0o?<\/strong><\/h3>\n\u0110\u1ec3 \u0111\u1ebfm c\u00e1c nhi\u1ec7m v\u1ee5 trong c\u00e1c l\u0129nh v\u1ef1c nh\u01b0 chuy\u1ec3n h\u00f3a ho\u1eb7c proteomics, HRMS gi\u00fap v\u1edbi s\u1ee9c m\u1ea1nh ch\u1ecdn h\u00e0ng \u0111\u1ea7u c\u1ee7a n\u00f3. N\u00f3 cho ph\u00e9p ng\u01b0\u1eddi lao \u0111\u1ed9ng ch\u1ec9 ra c\u00e1c m\u1ee5c ti\u00eau gi\u1eefa c\u00e1c c\u1ea5u tr\u00fac t\u01b0\u01a1ng t\u1ef1 ho\u1eb7c s\u1ef1 l\u1ed9n x\u1ed9n n\u1ec1n. SRM l\u00e0m cho vi\u1ec7c \u0111\u1ebfm nh\u1ea1y c\u1ea3m v\u00e0 \u1ed5n \u0111\u1ecbnh c\u00f3 th\u1ec3, ngay c\u1ea3 trong c\u00e1c m\u1eabu \u0111\u01b0\u1ee3c t\u1ea3i v\u1edbi c\u00e1c ph\u1ee5 ki\u1ec7n n\u01a1i m\u1ee5c ti\u00eau m \/ z ph\u00f9 h\u1ee3p v\u1edbi c\u00e1c ch\u1ea5t can thi\u1ec7p. Nh\u01b0 v\u1eady, HRMS ph\u1ee5c v\u1ee5 t\u1ed1t trong c\u00e1c th\u1eed nghi\u1ec7m cho d\u1ea5u v\u1ebft nh\u1ecf c\u1ee7a c\u00e1c v\u1eadt ch\u1ea5t kh\u00f4ng mong mu\u1ed1n ho\u1eb7c d\u01b0 th\u1eeba. N\u00f3 \u0111\u1ea3m b\u1ea3o c\u00e1c con s\u1ed1 \u0111\u00e1ng tin c\u1eady trong c\u00f4ng vi\u1ec7c \u0111\u01b0\u1ee3c quy \u0111\u1ecbnh.<\/p>\n
K\u1ef9 thu\u1eadt \u0111\u1ec3 t\u1ed1i \u01b0u h\u00f3a ho\u1eb7c c\u1ea3i thi\u1ec7n hi\u1ec7u su\u1ea5t \u0111\u1ed9 ph\u00e2n gi\u1ea3i MS<\/strong><\/h2>\n\u0110i\u1ec1u ch\u1ec9nh \u0111\u01b0\u1eddng d\u1eabn ion, v\u1edbi ki\u1ec3m so\u00e1t ch\u00ednh x\u00e1c c\u1ee7a \u0111i\u1ec7n \u00e1p tr\u00ean \u1ed1ng k\u00ednh \u0111i\u1ec1u khi\u1ec3n ion, c\u1eaft gi\u1ea3m s\u1ef1 ph\u00e2n t\u00e1n v\u00e0 s\u1eafc n\u00e9t tr\u1ecdng t\u00e2m. Ngo\u00e0i ra, c\u00e1c thi\u1ebft l\u1eadp trong m\u00e1y ph\u00e2n t\u00edch, nh\u01b0 \u0111\u01b0\u1eddng d\u00e0i h\u01a1n trong TOF-MS ho\u1eb7c c\u00e1c tr\u01b0\u1eddng m\u1ea1nh h\u01a1n trong FT-ICR, n\u00e2ng \u0111\u1ed9 ph\u00e2n gi\u1ea3i r\u00f5 r\u00e0ng. M\u00e1y ph\u00e2n t\u00edch t\u1ee9 c\u1ef1c v\u1edbi c\u00e1c b\u1ed9 ph\u1eadn l\u1ecdc ph\u00eda tr\u01b0\u1edbc c\u00f3 th\u1ec3 gi\u1ea3m s\u1ef1 t\u00edch t\u1ee5 b\u1ee5i b\u1ea9n. \u0110i\u1ec1u n\u00e0y t\u0103ng c\u01b0\u1eddng ch\u1ea1y \u1ed5n \u0111\u1ecbnh v\u00e0 \u00edt th\u1eddi gian d\u1eebng l\u1ea1i h\u01a1n. C\u00e1c t\u00ednh n\u0103ng n\u00e0y xu\u1ea5t hi\u1ec7n trong c\u00e1c c\u00f4ng c\u1ee5 nh\u01b0 M7 t\u1ee9 c\u1ef1c \u0111\u01a1n GC-MS<\/u><\/strong><\/a>. N\u00f3 c\u00f3 b\u1ed9 l\u1ecdc tr\u01b0\u1edbc b\u1ed1n c\u1ef1c k\u00e9o ra gi\u1eef cho ph\u1ea7n ch\u00ednh s\u1ea1ch s\u1ebd v\u00e0 b\u1ecf qua vi\u1ec7c l\u00e0m s\u1ea1ch th\u01b0\u1eddng xuy\u00ean.<\/p>\nL\u00e0m th\u1ebf n\u00e0o c\u00e1c c\u00f4ng c\u1ee5 ph\u1ea7n m\u1ec1m c\u00f3 th\u1ec3 c\u1ea3i thi\u1ec7n \u0111\u1ed9 ph\u00e2n gi\u1ea3i sau khi mua l\u1ea1i?<\/strong><\/h3>\nC\u00e1c ph\u00e2n chia \u0111\u1ec9nh d\u1ef1a tr\u00ean to\u00e1n h\u1ecdc gi\u1ea3i quy\u1ebft c\u00e1c \u0111\u1ec9nh pha tr\u1ed9n b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng c\u00e1c h\u00ecnh d\u1ea1ng v\u00e0 ph\u1ed5 bi\u1ebfn \u0111\u1ed3ng v\u1ecb d\u1ef1 ki\u1ebfn \u200b\u200btrong d\u1eef li\u1ec7u. C\u00e1c ph\u01b0\u01a1ng ph\u00e1p \u0111\u1ec3 c\u1eaft gi\u1ea3m ti\u1ebfng \u1ed3n, ch\u1eb3ng h\u1ea1n nh\u01b0 l\u00e0m m\u1ecbn k\u1ef9 thu\u1eadt s\u1ed1 v\u00e0 lo\u1ea1i b\u1ecf s\u1ef1 tr\u00f4i d\u1ea1t c\u01a1 b\u1ea3n, c\u0169ng l\u00e0m r\u00f5 c\u00e1c t\u00edn hi\u1ec7u. H\u1ecd t\u0103ng c\u01b0\u1eddng s\u1ef1 r\u00f5 r\u00e0ng gi\u00e1n ti\u1ebfp, h\u1ed7 tr\u1ee3 gi\u1ea3i quy\u1ebft. Th\u00f4ng th\u01b0\u1eddng, c\u00e1c c\u00f4ng c\u1ee5 n\u00e0y n\u1eb1m trong c\u00e1c b\u1ed9 ph\u1ea7n m\u1ec1m \u0111\u1ea7y \u0111\u1ee7 \u0111\u1ec3 gi\u00e1m s\u00e1t tr\u1ef1c ti\u1ebfp. V\u00ed d\u1ee5: G5 GC<\/u><\/strong><\/a> \u0111i k\u00e8m v\u1edbi ph\u1ea7n m\u1ec1m th\u00f4ng minh ch\u1ed1ng l\u1ea1i c\u00e1c v\u1ea5n \u0111\u1ec1 ki\u1ec3m so\u00e1t. N\u00f3 x\u1eed l\u00fd k\u1ebf ho\u1ea1ch nhi\u1ec7t \u0111\u1ed9, \u0111\u1ed3ng h\u1ed3 d\u00f2ng ch\u1ea3y v\u00e0 h\u1ed7 tr\u1ee3 m\u1ed9t s\u1ed1 m\u00e1y d\u00f2. Thi\u1ebft l\u1eadp n\u00e0y l\u00e0m cho vi\u1ec7c x\u1eed l\u00fd tr\u01a1n tru h\u01a1n v\u00e0 hi\u1ec7u qu\u1ea3 h\u01a1n.<\/p>\nPERSEE: M\u1ed9t \u0111\u1ed1i t\u00e1c \u0111\u00e1ng tin c\u1eady trong c\u00e1c gi\u1ea3i ph\u00e1p thi\u1ebft b\u1ecb ph\u00e2n t\u00edch<\/strong><\/h2>\nPERSEE<\/strong><\/a> B\u1eaft \u0111\u1ea7u v\u00e0o n\u0103m 1991 nh\u01b0 m\u1ed9t c\u00f4ng ty c\u00f4ng ngh\u1ec7 cao m\u1edbi<\/strong><\/a>N\u00f3 pha tr\u1ed9n nghi\u00ean c\u1ee9u, x\u00e2y d\u1ef1ng v\u00e0 b\u00e1n c\u00e1c c\u00f4ng c\u1ee5 khoa h\u1ecdc. V\u1edbi nhi\u1ec1u n\u0103m ki\u1ebfn th\u1ee9c trong c\u00e1c l\u0129nh v\u1ef1c nh\u01b0 quang ph\u1ed5 ph\u00e2n t\u1eed, nhi\u1ec5m s\u1eafc h\u1ecdc, quang ph\u1ed5 nguy\u00ean t\u1eed v\u00e0 h\u01a1n th\u1ebf n\u1eefa, n\u00f3 cung c\u1ea5p c\u00e2u tr\u1ea3 l\u1eddi \u0111\u1ea7y \u0111\u1ee7 cho c\u00e1c l\u0129nh v\u1ef1c nh\u01b0 d\u01b0\u1ee3c ph\u1ea9m, ki\u1ec3m tra m\u00f4i tr\u01b0\u1eddng, h\u00f3a d\u1ea7u, n\u00f4ng nghi\u1ec7p v\u00e0 gi\u1ea3ng d\u1ea1y.<\/p>\n\u0110i\u1ec1u l\u00e0m cho PERSEE \u0111\u1eb7c bi\u1ec7t l\u00e0 t\u1eadp trung v\u00e0o vi\u1ec7c t\u1ea1o ra c\u00e1c c\u00f4ng c\u1ee5 ch\u00ednh x\u00e1c v\u1edbi s\u1ef1 gi\u00fap \u0111\u1ee1 tr\u00ean to\u00e0n th\u1ebf gi\u1edbi. C\u00e1c \u00fd t\u01b0\u1edfng m\u1edbi c\u1ee7a n\u00f3, t\u1eeb m\u00e1y quang ph\u1ed5 UV-Vis \u0111\u1ebfn m\u00e1y nhi\u1ec5m s\u1eafc kh\u00ed chi ti\u1ebft, cho th\u1ea5y m\u1ed9t c\u00e1i nh\u00ecn th\u00f4ng minh. H\u1ecd gi\u1ea3i quy\u1ebft c\u00e1c v\u1ea5n \u0111\u1ec1 hi\u1ec7n t\u1ea1i trong ph\u00e2n t\u00edch v\u00e0 l\u1eadp k\u1ebf ho\u1ea1ch cho nh\u1eefng g\u00ec’ S ti\u1ebfp theo. M\u1ecdi ng\u01b0\u1eddi tr\u00ean to\u00e0n c\u1ea7u d\u1ef1a v\u00e0o c\u00e1c s\u1ea3n ph\u1ea9m PERSEE cho k\u1ebft qu\u1ea3 \u0111\u00fang \u0111\u1eafn, x\u00e2y d\u1ef1ng kh\u00f3 kh\u0103n v\u00e0 d\u1ec5 s\u1eed d\u1ee5ng.<\/p>\n
Nh\u1eadn x\u00e9t t\u00f3m t\u1eaft v\u1ec1 \u0111\u1ecbnh ngh\u0129a v\u00e0 \u00e1p d\u1ee5ng Ngh\u1ecb quy\u1ebft HRMS<\/strong><\/h2>\n\u0110\u1ed9 ph\u00e2n gi\u1ea3i l\u00e0 trung t\u00e2m c\u1ee7a c\u00f4ng ngh\u1ec7 HRMS. N\u00f3 mang l\u1ea1i \u0111\u1ed9 ch\u00ednh x\u00e1c h\u00e0ng \u0111\u1ea7u \u0111\u1ec3 ph\u00e1t hi\u1ec7n v\u00e0 \u0111\u1ebfm c\u00e1c nhi\u1ec7m v\u1ee5. C\u00e1c b\u1ed9 ph\u1eadn c\u1ee7a thi\u1ebft b\u1ecb, nh\u01b0 phong c\u00e1ch ph\u00e2n t\u00edch, thi\u1ebft l\u1eadp \u0111\u01b0\u1eddng d\u1eabn ion v\u00e0 b\u1ed9 b\u1eaft t\u00edn hi\u1ec7u, x\u00e2y d\u1ef1ng c\u01a1 s\u1edf cho vi\u1ec7c n\u00f3 gi\u1ea3i quy\u1ebft t\u1ed1t nh\u01b0 th\u1ebf n\u00e0o. C\u00e0i \u0111\u1eb7t trong khi ch\u1ea1y, ch\u1eb3ng h\u1ea1n nh\u01b0 t\u1ed1c \u0111\u1ed9 qu\u00e9t v\u00e0 \u0111i\u1ec1u ki\u1ec7n ngu\u1ed3n, c\u1ea7n ph\u00f9 h\u1ee3p \u0111\u1ec3 t\u1eadn d\u1ee5ng t\u1ed1i \u0111a. Cu\u1ed1i c\u00f9ng, \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao nh\u1ea5t \u0111\u1ebfn t\u1eeb vi\u1ec7c pha tr\u1ed9n ch\u0103m s\u00f3c ph\u1ea7n c\u1ee9ng v\u1edbi c\u00e1c b\u01b0\u1edbc ph\u1ea7n m\u1ec1m th\u00f4ng minh. H\u1ed7n h\u1ee3p n\u00e0y m\u1edf r\u1ed9ng nh\u1eefng g\u00ec quang ph\u1ed5 kh\u1ed1i l\u01b0\u1ee3ng c\u00f3 th\u1ec3 l\u00e0m trong c\u00e1c khu v\u1ef1c c\u1ea7n k\u1ebft qu\u1ea3 ch\u1eafc ch\u1eafn \u1edf m\u1ee9c th\u1ea5p.<\/p>\n
C\u00e2u h\u1ecfi th\u01b0\u1eddng g\u1eb7p<\/strong><\/h2>\nQ1: Gi\u00e1 tr\u1ecb \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao trong ph\u1ed5 \u0111o kh\u1ed1i l\u01b0\u1ee3ng \u0111\u01b0\u1ee3c coi l\u00e0 g\u00ec?
\n<\/strong>A1: Th\u00f4ng th\u01b0\u1eddng, c\u00f4ng su\u1ea5t gi\u1ea3i quy\u1ebft l\u1edbn h\u01a1n 10.000 (m \/ \u0394m t\u1ea1i FWHM) \u0111\u01b0\u1ee3c coi l\u00e0 \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao; M\u1ed9t s\u1ed1 thi\u1ebft b\u1ecb v\u01b0\u1ee3t qu\u00e1 100.000 t\u00f9y thu\u1ed9c v\u00e0o lo\u1ea1i m\u00e1y ph\u00e2n t\u00edch.<\/p>\nQ2: Ph\u1ed5 \u0111o kh\u1ed1i l\u01b0\u1ee3ng \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng cho ph\u00e2n t\u00edch \u0111\u1ecbnh l\u01b0\u1ee3ng th\u01b0\u1eddng xuy\u00ean kh\u00f4ng?
\n<\/strong>A2: V\u00e2ng, HRMS cung c\u1ea5p t\u00ednh ch\u1ecdn l\u1ecdc tuy\u1ec7t v\u1eddi n\u00e2ng cao \u0111\u1ed9 ch\u00ednh x\u00e1c \u0111\u1ecbnh l\u01b0\u1ee3ng ngay c\u1ea3 trong ma tr\u1eadn ph\u1ee9c t\u1ea1p; Tuy nhi\u00ean, vi\u1ec7c ph\u00e1t tri\u1ec3n ph\u01b0\u01a1ng ph\u00e1p ph\u1ea3i t\u00ednh \u0111\u1ebfn c\u00e1c th\u00f4ng s\u1ed1 c\u1ee5 th\u1ec3 c\u1ee7a d\u1ee5ng c\u1ee5.<\/p>\nQ3: M\u1ed9t d\u1ee5ng c\u1ee5 \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao n\u00ean \u0111\u01b0\u1ee3c hi\u1ec7u chu\u1ea9n bao l\u00e2u?
\n<\/strong>A3: T\u1ea7n s\u1ed1 hi\u1ec7u chu\u1ea9n ph\u1ee5 thu\u1ed9c v\u00e0o c\u01b0\u1eddng \u0111\u1ed9 s\u1eed d\u1ee5ng nh\u01b0ng n\u00f3i chung n\u00ean \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n h\u00e0ng ng\u00e0y ho\u1eb7c tr\u01b0\u1edbc m\u1ed7i l\u00f4 ph\u00e2n t\u00edch \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o vi\u1ec7c g\u00e1n kh\u1ed1i l\u01b0\u1ee3ng ch\u00ednh x\u00e1c v\u00e0 hi\u1ec7u su\u1ea5t nh\u1ea5t qu\u00e1n.<\/p>","protected":false},"excerpt":{"rendered":" In high resolution mass spectrometry (HRMS), resolution means the device’s skill in telling apart ions with almost the same mass-to-charge (m\/z) values. This skill matters a lot for spotting compounds correctly, particularly in tricky mixtures. In math terms, resolution shows as R = m\/\u0394m. Here, “m” stands for the m\/z at the main peak, […]<\/p>\n","protected":false},"author":1,"featured_media":4261,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[38,37],"tags":[],"class_list":["post-4265","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-industry-information","category-news"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts\/4265","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/comments?post=4265"}],"version-history":[{"count":4,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts\/4265\/revisions"}],"predecessor-version":[{"id":4273,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts\/4265\/revisions\/4273"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/media\/4261"}],"wp:attachment":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/media?parent=4265"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/categories?post=4265"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/tags?post=4265"}],"curies":[{"name":"WP","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
C\u00e1ch m\u00e1y ph\u00e2n t\u00edch kh\u1ed1i l\u01b0\u1ee3ng \u0111\u01b0\u1ee3c x\u00e2y d\u1ef1ng r\u1ea5t quan tr\u1ecdng. H\u1ea7u h\u1ebft c\u00e1c \u0111\u01a1n v\u1ecb TOFMS, ch\u1eb3ng h\u1ea1n nh\u01b0 t\u1eeb JEOL, cung c\u1ea5p chi ti\u1ebft kh\u1ed1i l\u01b0\u1ee3ng v\u1eefng ch\u1eafc v\u00e0 \u0111\u1ecdc kh\u1ed1i l\u01b0\u1ee3ng ch\u00ednh x\u00e1c. B\u1edfi v\u00ec \u0111i\u1ec1u n\u00e0y, h\u1ecd gi\u00fap t\u00ecm ra trang \u0111i\u1ec3m nguy\u00ean t\u1ed1 th\u00f4ng qua ki\u1ec3m tra kh\u1ed1i l\u01b0\u1ee3ng c\u1ea9n th\u1eadn. B\u00ean c\u1ea1nh \u0111\u00f3, c\u00e1c con \u0111\u01b0\u1eddng ion v\u00e0 c\u00e1ch ion di chuy\u1ec3n \u0111\u1ecbnh h\u00ecnh tr\u1ecdng t\u00e2m tr\u01b0\u1edbc khi ch\u00fang \u0111\u00e2m v\u00e0o m\u00e1y d\u00f2. C\u00e1c b\u1ed9 ph\u1eadn ph\u00e1t hi\u1ec7n v\u00e0 t\u0103ng c\u01b0\u1eddng t\u00edn hi\u1ec7u, nh\u01b0 \u0111i\u1ec7n t\u1eed \u0111\u1ec3 khu\u1ebfch \u0111\u1ea1i v\u00e0 t\u1ea7n su\u1ea5t ch\u00fang l\u1ea5y m\u1eabu, c\u0169ng \u0111\u00f3ng m\u1ed9t vai tr\u00f2. Ch\u00fang gi\u1eef cho th\u00f4ng tin ion r\u00f5 r\u00e0ng, h\u1ed7 tr\u1ee3 \u0111\u1ed9 ph\u00e2n gi\u1ea3i t\u1ed5ng th\u1ec3 t\u1ed1t h\u01a1n.<\/p>\n
\u0110i\u1ec1u ki\u1ec7n ho\u1ea1t \u0111\u1ed9ng thay \u0111\u1ed5i \u0111\u1ed9 ph\u00e2n gi\u1ea3i thi\u1ebft b\u1ecb nh\u01b0 th\u1ebf n\u00e0o?<\/strong><\/h3>\nC\u00e0i \u0111\u1eb7t trong ngu\u1ed3n ion, bao g\u1ed3m nhi\u1ec7t, c\u01b0\u1eddng \u0111\u1ed9 electron v\u00e0 \u00e1p su\u1ea5t kh\u00f4ng kh\u00ed, thay \u0111\u1ed5i c\u00e1ch ion h\u00ecnh th\u00e0nh v\u00e0 lan r\u1ed9ng. Nhi\u1ec7t \u0111\u1ed9 \u1ea5m h\u01a1n v\u00e0 \u0111i\u1ec7n \u00e1p \u0111\u01b0\u1ee3c \u0111i\u1ec1u ch\u1ec9nh tinh t\u1ebf l\u00e0m cho ion h\u00f3a \u0111\u1ed3ng \u0111\u1ec1u h\u01a1n. Tuy nhi\u00ean, b\u1ea1n c\u1ea7n theo d\u00f5i ch\u00fang ch\u1eb7t ch\u1ebd \u0111\u1ec3 ng\u0103n ch\u1eb7n s\u1ef1 ph\u00e1 v\u1ee1 kh\u00f4ng mong mu\u1ed1n trong c\u00e1c ph\u00e2n t\u1eed ho\u1eb7c t\u00edn hi\u1ec7u y\u1ebfu h\u01a1n.<\/p>\n
M\u1ed9t \u0111i\u1ec3m quan tr\u1ecdng kh\u00e1c l\u00e0 thi\u1ebft b\u1ecb qu\u00e9t nhanh nh\u01b0 th\u1ebf n\u00e0o. Qu\u00e9t nhanh gi\u1ea3m th\u1eddi gian \u0111\u1ec3 l\u1ea5y chi ti\u1ebft t\u00edn hi\u1ec7u, c\u00f3 th\u1ec3 gi\u1ea3m \u0111\u1ed9 ph\u00e2n gi\u1ea3i. \u0110\u1ed3ng th\u1eddi, nh\u1eefng th\u1ee9 trong m\u1eabu, nh\u01b0 ma tr\u1eadn, c\u00f3 th\u1ec3 ch\u1eb7n c\u00e1c ion. \u0110i\u1ec1u n\u00e0y d\u1eabn \u0111\u1ebfn c\u00e1c \u0111\u1ec9nh r\u1ed9ng h\u01a1n ho\u1eb7c cong, c\u1eaft gi\u1ea3m thi\u1ebft b\u1ecb’ s t\u00e1ch \u0111i\u1ec7n. Do \u0111\u00f3, \u0111i\u1ec1u ch\u1ec9nh c\u1ea9n th\u1eadn gi\u00fap duy tr\u00ec hi\u1ec7u su\u1ea5t m\u1ea1nh m\u1ebd.<\/p>\n
So s\u00e1nh gi\u1eefa \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao v\u00e0 \u0111\u1ed9 ph\u00e2n gi\u1ea3i th\u1ea5p<\/strong><\/h2>\nC\u00e1c thi\u1ebft b\u1ecb c\u00f3 \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao mang l\u1ea1i l\u1ee3i \u00edch l\u1edbn trong vi\u1ec7c ch\u1ecdn m\u1ee5c ti\u00eau v\u00e0 x\u00e2y d\u1ef1ng ni\u1ec1m tin v\u00e0o k\u1ebft qu\u1ea3. Ch\u00fang c\u00f3 th\u1ec3 chia kh\u1ed1i l\u01b0\u1ee3ng ngay c\u1ea3 cho c\u00e1c ion c\u00f3 c\u00f9ng m\u1ed9t s\u1ed1 kh\u1ed1i l\u01b0\u1ee3ng c\u01a1 b\u1ea3n. Ngo\u00e0i ra, ch\u00fang cho ph\u00e9p b\u1ea1n l\u00e0m vi\u1ec7c ra trang \u0111i\u1ec3m c\u1ee7a c\u00e1c y\u1ebfu t\u1ed1. \u0110i\u1ec1u n\u00e0y ch\u1ee9ng minh r\u1ea5t h\u1eefu \u00edch trong c\u00e1c m\u1eabu \u0111\u1ea7y \u0111\u1ee7 tr\u00f4ng gi\u1ed1ng nhau ho\u1eb7c khi t\u00ecm ki\u1ebfm m\u00e0 kh\u00f4ng c\u00f3 danh s\u00e1ch c\u00e1c h\u1ee3p ch\u1ea5t \u0111\u01b0\u1ee3c thi\u1ebft l\u1eadp. Ngo\u00e0i ra, c\u00e1c h\u1ec7 th\u1ed1ng n\u00e0y cung c\u1ea5p \u0111\u1ed9 ch\u00ednh x\u00e1c kh\u1ed1i l\u01b0\u1ee3ng t\u1ed1t h\u01a1n. \u0110i\u1ec1u \u0111\u00f3 l\u00e0m t\u0103ng s\u1ef1 ch\u1eafc ch\u1eafn trong vi\u1ec7c li\u00ean k\u1ebft v\u1edbi c\u00e1c c\u00f4ng th\u1ee9c ph\u00e2n t\u1eed. C\u00e1c t\u00ednh n\u0103ng nh\u01b0 v\u1eady r\u1ea5t quan tr\u1ecdng trong c\u00e1c l\u0129nh v\u1ef1c nh\u01b0 theo d\u00f5i m\u00f4i tr\u01b0\u1eddng ho\u1eb7c ki\u1ec3m tra ch\u1ea5t l\u01b0\u1ee3ng thu\u1ed1c. N\u00f3i t\u00f3m l\u1ea1i, \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao t\u0103ng c\u01b0\u1eddng tr\u00f2 ch\u01a1i cho c\u00e1c ph\u00e2n t\u00edch kh\u00f3 kh\u0103n.<\/p>\n
Nh\u1eefng h\u1ea1n ch\u1ebf c\u1ee7a vi\u1ec7c s\u1eed d\u1ee5ng MS \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao l\u00e0 g\u00ec?<\/strong><\/h3>\nNgay c\u1ea3 v\u1edbi \u0111i\u1ec3m m\u1ea1nh c\u1ee7a h\u1ecd, c\u00e1c thi\u1ebft l\u1eadp \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao chi ph\u00ed nhi\u1ec1u h\u01a1n v\u00e0 m\u1ea5t nhi\u1ec1u n\u1ed7 l\u1ef1c \u0111\u1ec3 ch\u1ea1y h\u01a1n nh\u1eefng thi\u1ebft l\u1eadp c\u01a1 b\u1ea3n. H\u1ecd c\u1ea7n c\u00e1c b\u01b0\u1edbc \u0111i\u1ec1u ch\u1ec9nh \u0111\u1eb7c bi\u1ec7t \u0111\u1ec3 ti\u1ebfp t\u1ee5c ho\u1ea1t \u0111\u1ed9ng t\u1ed1t. Ngo\u00e0i ra, h\u1ecd ph\u1ee5 thu\u1ed9c v\u00e0o ph\u1ea7n m\u1ec1m th\u00f4ng minh \u0111\u1ec3 hi\u1ec3u \u00fd ngh\u0129a c\u1ee7a d\u1eef li\u1ec7u. C\u00e1c \u0111\u01a1n v\u1ecb nh\u01b0 Orbitraps ho\u1eb7c FT-ICR y\u00eau c\u1ea7u ki\u1ec3m so\u00e1t ch\u1eb7t ch\u1ebd m\u00f4i tr\u01b0\u1eddng xung quanh v\u00e0 b\u1ea3o tr\u00ec \u1ed5n \u0111\u1ecbnh. \u0110i\u1ec1u g\u00ec’ H\u01a1n n\u1eefa, l\u01b0\u1ee3ng d\u1eef li\u1ec7u kh\u1ed5ng l\u1ed3 t\u1eeb c\u00e1c ch\u1ea1y n\u00e0y \u0111\u00f2i h\u1ecfi c\u00e1c m\u00e1y t\u00ednh m\u1ea1nh m\u1ebd \u0111\u1ec3 x\u1eed l\u00fd t\u1ea5t c\u1ea3. V\u00ec v\u1eady, trong khi m\u1ea1nh m\u1ebd, h\u1ecd \u0111\u00f2i h\u1ecfi nhi\u1ec1u ngu\u1ed3n l\u1ef1c h\u01a1n t\u1ed5ng th\u1ec3.<\/p>\n
C\u00e1c \u1ee9ng d\u1ee5ng y\u00eau c\u1ea7u ph\u1ed5 \u0111o kh\u1ed1i l\u01b0\u1ee3ng \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao<\/strong><\/h2>\n <\/p>\n
<\/div>\nTrong c\u00e1c m\u00f4i tr\u01b0\u1eddng kh\u00f3 kh\u0103n nh\u01b0 c\u00e1c m\u1eabu m\u00f4i tr\u01b0\u1eddng ho\u1eb7c cu\u1ed9c s\u1ed1ng v\u1edbi nhi\u1ec1u h\u1ee3p ch\u1ea5t tr\u1ed9n v\u1edbi nhau, HRMS n\u1ed5i b\u1eadt \u0111\u1ec3 t\u00ecm nh\u1eefng \u0111i\u1ec1u ch\u01b0a bi\u1ebft m\u00e0 kh\u00f4ng ch\u1ec9 ph\u00f9 h\u1ee3p v\u1edbi danh s\u00e1ch. M\u1ed9t TOFMS chi ti\u1ebft c\u00f3 th\u1ec3 t\u1ef1 ph\u00e1t hi\u1ec7n c\u00e1c m\u1ee5c kh\u00f4ng bi\u1ebft, m\u00e0 kh\u00f4ng c\u1ea7n s\u1ef1 gi\u00fap \u0111\u1ee1 c\u1ee7a th\u01b0 vi\u1ec7n. N\u00f3 ph\u00f9 h\u1ee3p v\u1edbi c\u00e1c t\u00ecm ki\u1ebfm r\u1ed9ng r\u00e3i. C\u00e1c m\u1ea3nh kh\u1ed1i l\u01b0\u1ee3ng ch\u00ednh x\u00e1c v\u00e0 c\u00e1c d\u1ea5u hi\u1ec7u \u0111\u1ed3ng v\u1ecb th\u00eam v\u00e0o vi\u1ec7c t\u00ecm ra c\u1ea5u tr\u00fac. C\u00e1ch ti\u1ebfp c\u1eadn n\u00e0y t\u1ecfa s\u00e1ng b\u1edfi v\u00ec n\u00f3 x\u1eed l\u00fd s\u1ef1 ph\u1ee9c t\u1ea1p tr\u1ef1c ti\u1ebfp. V\u00ed d\u1ee5, trong c\u00e1c m\u1eabu \u0111\u00f4ng \u0111\u00fac, n\u00f3 k\u00e9o ra c\u00e1c chi ti\u1ebft m\u00e0 c\u00e1c c\u00f4ng c\u1ee5 \u0111\u01a1n gi\u1ea3n h\u01a1n b\u1ecf l\u1ee1. Do \u0111\u00f3, n\u00f3 h\u1ed7 tr\u1ee3 c\u00e1i nh\u00ecn s\u00e2u s\u1eafc h\u01a1n v\u1ec1 nh\u1eefng g\u00ec’ S hi\u1ec7n t\u1ea1i.<\/p>\n
HRMS c\u1ea3i thi\u1ec7n quy tr\u00ecnh l\u00e0m vi\u1ec7c \u0111\u1ecbnh l\u01b0\u1ee3ng nh\u01b0 th\u1ebf n\u00e0o?<\/strong><\/h3>\n\u0110\u1ec3 \u0111\u1ebfm c\u00e1c nhi\u1ec7m v\u1ee5 trong c\u00e1c l\u0129nh v\u1ef1c nh\u01b0 chuy\u1ec3n h\u00f3a ho\u1eb7c proteomics, HRMS gi\u00fap v\u1edbi s\u1ee9c m\u1ea1nh ch\u1ecdn h\u00e0ng \u0111\u1ea7u c\u1ee7a n\u00f3. N\u00f3 cho ph\u00e9p ng\u01b0\u1eddi lao \u0111\u1ed9ng ch\u1ec9 ra c\u00e1c m\u1ee5c ti\u00eau gi\u1eefa c\u00e1c c\u1ea5u tr\u00fac t\u01b0\u01a1ng t\u1ef1 ho\u1eb7c s\u1ef1 l\u1ed9n x\u1ed9n n\u1ec1n. SRM l\u00e0m cho vi\u1ec7c \u0111\u1ebfm nh\u1ea1y c\u1ea3m v\u00e0 \u1ed5n \u0111\u1ecbnh c\u00f3 th\u1ec3, ngay c\u1ea3 trong c\u00e1c m\u1eabu \u0111\u01b0\u1ee3c t\u1ea3i v\u1edbi c\u00e1c ph\u1ee5 ki\u1ec7n n\u01a1i m\u1ee5c ti\u00eau m \/ z ph\u00f9 h\u1ee3p v\u1edbi c\u00e1c ch\u1ea5t can thi\u1ec7p. Nh\u01b0 v\u1eady, HRMS ph\u1ee5c v\u1ee5 t\u1ed1t trong c\u00e1c th\u1eed nghi\u1ec7m cho d\u1ea5u v\u1ebft nh\u1ecf c\u1ee7a c\u00e1c v\u1eadt ch\u1ea5t kh\u00f4ng mong mu\u1ed1n ho\u1eb7c d\u01b0 th\u1eeba. N\u00f3 \u0111\u1ea3m b\u1ea3o c\u00e1c con s\u1ed1 \u0111\u00e1ng tin c\u1eady trong c\u00f4ng vi\u1ec7c \u0111\u01b0\u1ee3c quy \u0111\u1ecbnh.<\/p>\n
K\u1ef9 thu\u1eadt \u0111\u1ec3 t\u1ed1i \u01b0u h\u00f3a ho\u1eb7c c\u1ea3i thi\u1ec7n hi\u1ec7u su\u1ea5t \u0111\u1ed9 ph\u00e2n gi\u1ea3i MS<\/strong><\/h2>\n\u0110i\u1ec1u ch\u1ec9nh \u0111\u01b0\u1eddng d\u1eabn ion, v\u1edbi ki\u1ec3m so\u00e1t ch\u00ednh x\u00e1c c\u1ee7a \u0111i\u1ec7n \u00e1p tr\u00ean \u1ed1ng k\u00ednh \u0111i\u1ec1u khi\u1ec3n ion, c\u1eaft gi\u1ea3m s\u1ef1 ph\u00e2n t\u00e1n v\u00e0 s\u1eafc n\u00e9t tr\u1ecdng t\u00e2m. Ngo\u00e0i ra, c\u00e1c thi\u1ebft l\u1eadp trong m\u00e1y ph\u00e2n t\u00edch, nh\u01b0 \u0111\u01b0\u1eddng d\u00e0i h\u01a1n trong TOF-MS ho\u1eb7c c\u00e1c tr\u01b0\u1eddng m\u1ea1nh h\u01a1n trong FT-ICR, n\u00e2ng \u0111\u1ed9 ph\u00e2n gi\u1ea3i r\u00f5 r\u00e0ng. M\u00e1y ph\u00e2n t\u00edch t\u1ee9 c\u1ef1c v\u1edbi c\u00e1c b\u1ed9 ph\u1eadn l\u1ecdc ph\u00eda tr\u01b0\u1edbc c\u00f3 th\u1ec3 gi\u1ea3m s\u1ef1 t\u00edch t\u1ee5 b\u1ee5i b\u1ea9n. \u0110i\u1ec1u n\u00e0y t\u0103ng c\u01b0\u1eddng ch\u1ea1y \u1ed5n \u0111\u1ecbnh v\u00e0 \u00edt th\u1eddi gian d\u1eebng l\u1ea1i h\u01a1n. C\u00e1c t\u00ednh n\u0103ng n\u00e0y xu\u1ea5t hi\u1ec7n trong c\u00e1c c\u00f4ng c\u1ee5 nh\u01b0 M7 t\u1ee9 c\u1ef1c \u0111\u01a1n GC-MS<\/u><\/strong><\/a>. N\u00f3 c\u00f3 b\u1ed9 l\u1ecdc tr\u01b0\u1edbc b\u1ed1n c\u1ef1c k\u00e9o ra gi\u1eef cho ph\u1ea7n ch\u00ednh s\u1ea1ch s\u1ebd v\u00e0 b\u1ecf qua vi\u1ec7c l\u00e0m s\u1ea1ch th\u01b0\u1eddng xuy\u00ean.<\/p>\nL\u00e0m th\u1ebf n\u00e0o c\u00e1c c\u00f4ng c\u1ee5 ph\u1ea7n m\u1ec1m c\u00f3 th\u1ec3 c\u1ea3i thi\u1ec7n \u0111\u1ed9 ph\u00e2n gi\u1ea3i sau khi mua l\u1ea1i?<\/strong><\/h3>\nC\u00e1c ph\u00e2n chia \u0111\u1ec9nh d\u1ef1a tr\u00ean to\u00e1n h\u1ecdc gi\u1ea3i quy\u1ebft c\u00e1c \u0111\u1ec9nh pha tr\u1ed9n b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng c\u00e1c h\u00ecnh d\u1ea1ng v\u00e0 ph\u1ed5 bi\u1ebfn \u0111\u1ed3ng v\u1ecb d\u1ef1 ki\u1ebfn \u200b\u200btrong d\u1eef li\u1ec7u. C\u00e1c ph\u01b0\u01a1ng ph\u00e1p \u0111\u1ec3 c\u1eaft gi\u1ea3m ti\u1ebfng \u1ed3n, ch\u1eb3ng h\u1ea1n nh\u01b0 l\u00e0m m\u1ecbn k\u1ef9 thu\u1eadt s\u1ed1 v\u00e0 lo\u1ea1i b\u1ecf s\u1ef1 tr\u00f4i d\u1ea1t c\u01a1 b\u1ea3n, c\u0169ng l\u00e0m r\u00f5 c\u00e1c t\u00edn hi\u1ec7u. H\u1ecd t\u0103ng c\u01b0\u1eddng s\u1ef1 r\u00f5 r\u00e0ng gi\u00e1n ti\u1ebfp, h\u1ed7 tr\u1ee3 gi\u1ea3i quy\u1ebft. Th\u00f4ng th\u01b0\u1eddng, c\u00e1c c\u00f4ng c\u1ee5 n\u00e0y n\u1eb1m trong c\u00e1c b\u1ed9 ph\u1ea7n m\u1ec1m \u0111\u1ea7y \u0111\u1ee7 \u0111\u1ec3 gi\u00e1m s\u00e1t tr\u1ef1c ti\u1ebfp. V\u00ed d\u1ee5: G5 GC<\/u><\/strong><\/a> \u0111i k\u00e8m v\u1edbi ph\u1ea7n m\u1ec1m th\u00f4ng minh ch\u1ed1ng l\u1ea1i c\u00e1c v\u1ea5n \u0111\u1ec1 ki\u1ec3m so\u00e1t. N\u00f3 x\u1eed l\u00fd k\u1ebf ho\u1ea1ch nhi\u1ec7t \u0111\u1ed9, \u0111\u1ed3ng h\u1ed3 d\u00f2ng ch\u1ea3y v\u00e0 h\u1ed7 tr\u1ee3 m\u1ed9t s\u1ed1 m\u00e1y d\u00f2. Thi\u1ebft l\u1eadp n\u00e0y l\u00e0m cho vi\u1ec7c x\u1eed l\u00fd tr\u01a1n tru h\u01a1n v\u00e0 hi\u1ec7u qu\u1ea3 h\u01a1n.<\/p>\nPERSEE: M\u1ed9t \u0111\u1ed1i t\u00e1c \u0111\u00e1ng tin c\u1eady trong c\u00e1c gi\u1ea3i ph\u00e1p thi\u1ebft b\u1ecb ph\u00e2n t\u00edch<\/strong><\/h2>\nPERSEE<\/strong><\/a> B\u1eaft \u0111\u1ea7u v\u00e0o n\u0103m 1991 nh\u01b0 m\u1ed9t c\u00f4ng ty c\u00f4ng ngh\u1ec7 cao m\u1edbi<\/strong><\/a>N\u00f3 pha tr\u1ed9n nghi\u00ean c\u1ee9u, x\u00e2y d\u1ef1ng v\u00e0 b\u00e1n c\u00e1c c\u00f4ng c\u1ee5 khoa h\u1ecdc. V\u1edbi nhi\u1ec1u n\u0103m ki\u1ebfn th\u1ee9c trong c\u00e1c l\u0129nh v\u1ef1c nh\u01b0 quang ph\u1ed5 ph\u00e2n t\u1eed, nhi\u1ec5m s\u1eafc h\u1ecdc, quang ph\u1ed5 nguy\u00ean t\u1eed v\u00e0 h\u01a1n th\u1ebf n\u1eefa, n\u00f3 cung c\u1ea5p c\u00e2u tr\u1ea3 l\u1eddi \u0111\u1ea7y \u0111\u1ee7 cho c\u00e1c l\u0129nh v\u1ef1c nh\u01b0 d\u01b0\u1ee3c ph\u1ea9m, ki\u1ec3m tra m\u00f4i tr\u01b0\u1eddng, h\u00f3a d\u1ea7u, n\u00f4ng nghi\u1ec7p v\u00e0 gi\u1ea3ng d\u1ea1y.<\/p>\n\u0110i\u1ec1u l\u00e0m cho PERSEE \u0111\u1eb7c bi\u1ec7t l\u00e0 t\u1eadp trung v\u00e0o vi\u1ec7c t\u1ea1o ra c\u00e1c c\u00f4ng c\u1ee5 ch\u00ednh x\u00e1c v\u1edbi s\u1ef1 gi\u00fap \u0111\u1ee1 tr\u00ean to\u00e0n th\u1ebf gi\u1edbi. C\u00e1c \u00fd t\u01b0\u1edfng m\u1edbi c\u1ee7a n\u00f3, t\u1eeb m\u00e1y quang ph\u1ed5 UV-Vis \u0111\u1ebfn m\u00e1y nhi\u1ec5m s\u1eafc kh\u00ed chi ti\u1ebft, cho th\u1ea5y m\u1ed9t c\u00e1i nh\u00ecn th\u00f4ng minh. H\u1ecd gi\u1ea3i quy\u1ebft c\u00e1c v\u1ea5n \u0111\u1ec1 hi\u1ec7n t\u1ea1i trong ph\u00e2n t\u00edch v\u00e0 l\u1eadp k\u1ebf ho\u1ea1ch cho nh\u1eefng g\u00ec’ S ti\u1ebfp theo. M\u1ecdi ng\u01b0\u1eddi tr\u00ean to\u00e0n c\u1ea7u d\u1ef1a v\u00e0o c\u00e1c s\u1ea3n ph\u1ea9m PERSEE cho k\u1ebft qu\u1ea3 \u0111\u00fang \u0111\u1eafn, x\u00e2y d\u1ef1ng kh\u00f3 kh\u0103n v\u00e0 d\u1ec5 s\u1eed d\u1ee5ng.<\/p>\n
Nh\u1eadn x\u00e9t t\u00f3m t\u1eaft v\u1ec1 \u0111\u1ecbnh ngh\u0129a v\u00e0 \u00e1p d\u1ee5ng Ngh\u1ecb quy\u1ebft HRMS<\/strong><\/h2>\n\u0110\u1ed9 ph\u00e2n gi\u1ea3i l\u00e0 trung t\u00e2m c\u1ee7a c\u00f4ng ngh\u1ec7 HRMS. N\u00f3 mang l\u1ea1i \u0111\u1ed9 ch\u00ednh x\u00e1c h\u00e0ng \u0111\u1ea7u \u0111\u1ec3 ph\u00e1t hi\u1ec7n v\u00e0 \u0111\u1ebfm c\u00e1c nhi\u1ec7m v\u1ee5. C\u00e1c b\u1ed9 ph\u1eadn c\u1ee7a thi\u1ebft b\u1ecb, nh\u01b0 phong c\u00e1ch ph\u00e2n t\u00edch, thi\u1ebft l\u1eadp \u0111\u01b0\u1eddng d\u1eabn ion v\u00e0 b\u1ed9 b\u1eaft t\u00edn hi\u1ec7u, x\u00e2y d\u1ef1ng c\u01a1 s\u1edf cho vi\u1ec7c n\u00f3 gi\u1ea3i quy\u1ebft t\u1ed1t nh\u01b0 th\u1ebf n\u00e0o. C\u00e0i \u0111\u1eb7t trong khi ch\u1ea1y, ch\u1eb3ng h\u1ea1n nh\u01b0 t\u1ed1c \u0111\u1ed9 qu\u00e9t v\u00e0 \u0111i\u1ec1u ki\u1ec7n ngu\u1ed3n, c\u1ea7n ph\u00f9 h\u1ee3p \u0111\u1ec3 t\u1eadn d\u1ee5ng t\u1ed1i \u0111a. Cu\u1ed1i c\u00f9ng, \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao nh\u1ea5t \u0111\u1ebfn t\u1eeb vi\u1ec7c pha tr\u1ed9n ch\u0103m s\u00f3c ph\u1ea7n c\u1ee9ng v\u1edbi c\u00e1c b\u01b0\u1edbc ph\u1ea7n m\u1ec1m th\u00f4ng minh. H\u1ed7n h\u1ee3p n\u00e0y m\u1edf r\u1ed9ng nh\u1eefng g\u00ec quang ph\u1ed5 kh\u1ed1i l\u01b0\u1ee3ng c\u00f3 th\u1ec3 l\u00e0m trong c\u00e1c khu v\u1ef1c c\u1ea7n k\u1ebft qu\u1ea3 ch\u1eafc ch\u1eafn \u1edf m\u1ee9c th\u1ea5p.<\/p>\n
C\u00e2u h\u1ecfi th\u01b0\u1eddng g\u1eb7p<\/strong><\/h2>\nQ1: Gi\u00e1 tr\u1ecb \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao trong ph\u1ed5 \u0111o kh\u1ed1i l\u01b0\u1ee3ng \u0111\u01b0\u1ee3c coi l\u00e0 g\u00ec?
\n<\/strong>A1: Th\u00f4ng th\u01b0\u1eddng, c\u00f4ng su\u1ea5t gi\u1ea3i quy\u1ebft l\u1edbn h\u01a1n 10.000 (m \/ \u0394m t\u1ea1i FWHM) \u0111\u01b0\u1ee3c coi l\u00e0 \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao; M\u1ed9t s\u1ed1 thi\u1ebft b\u1ecb v\u01b0\u1ee3t qu\u00e1 100.000 t\u00f9y thu\u1ed9c v\u00e0o lo\u1ea1i m\u00e1y ph\u00e2n t\u00edch.<\/p>\nQ2: Ph\u1ed5 \u0111o kh\u1ed1i l\u01b0\u1ee3ng \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng cho ph\u00e2n t\u00edch \u0111\u1ecbnh l\u01b0\u1ee3ng th\u01b0\u1eddng xuy\u00ean kh\u00f4ng?
\n<\/strong>A2: V\u00e2ng, HRMS cung c\u1ea5p t\u00ednh ch\u1ecdn l\u1ecdc tuy\u1ec7t v\u1eddi n\u00e2ng cao \u0111\u1ed9 ch\u00ednh x\u00e1c \u0111\u1ecbnh l\u01b0\u1ee3ng ngay c\u1ea3 trong ma tr\u1eadn ph\u1ee9c t\u1ea1p; Tuy nhi\u00ean, vi\u1ec7c ph\u00e1t tri\u1ec3n ph\u01b0\u01a1ng ph\u00e1p ph\u1ea3i t\u00ednh \u0111\u1ebfn c\u00e1c th\u00f4ng s\u1ed1 c\u1ee5 th\u1ec3 c\u1ee7a d\u1ee5ng c\u1ee5.<\/p>\nQ3: M\u1ed9t d\u1ee5ng c\u1ee5 \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao n\u00ean \u0111\u01b0\u1ee3c hi\u1ec7u chu\u1ea9n bao l\u00e2u?
\n<\/strong>A3: T\u1ea7n s\u1ed1 hi\u1ec7u chu\u1ea9n ph\u1ee5 thu\u1ed9c v\u00e0o c\u01b0\u1eddng \u0111\u1ed9 s\u1eed d\u1ee5ng nh\u01b0ng n\u00f3i chung n\u00ean \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n h\u00e0ng ng\u00e0y ho\u1eb7c tr\u01b0\u1edbc m\u1ed7i l\u00f4 ph\u00e2n t\u00edch \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o vi\u1ec7c g\u00e1n kh\u1ed1i l\u01b0\u1ee3ng ch\u00ednh x\u00e1c v\u00e0 hi\u1ec7u su\u1ea5t nh\u1ea5t qu\u00e1n.<\/p>","protected":false},"excerpt":{"rendered":" In high resolution mass spectrometry (HRMS), resolution means the device’s skill in telling apart ions with almost the same mass-to-charge (m\/z) values. This skill matters a lot for spotting compounds correctly, particularly in tricky mixtures. In math terms, resolution shows as R = m\/\u0394m. Here, “m” stands for the m\/z at the main peak, […]<\/p>\n","protected":false},"author":1,"featured_media":4261,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[38,37],"tags":[],"class_list":["post-4265","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-industry-information","category-news"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts\/4265","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/comments?post=4265"}],"version-history":[{"count":4,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts\/4265\/revisions"}],"predecessor-version":[{"id":4273,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts\/4265\/revisions\/4273"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/media\/4261"}],"wp:attachment":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/media?parent=4265"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/categories?post=4265"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/tags?post=4265"}],"curies":[{"name":"WP","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
C\u00e1c thi\u1ebft b\u1ecb c\u00f3 \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao mang l\u1ea1i l\u1ee3i \u00edch l\u1edbn trong vi\u1ec7c ch\u1ecdn m\u1ee5c ti\u00eau v\u00e0 x\u00e2y d\u1ef1ng ni\u1ec1m tin v\u00e0o k\u1ebft qu\u1ea3. Ch\u00fang c\u00f3 th\u1ec3 chia kh\u1ed1i l\u01b0\u1ee3ng ngay c\u1ea3 cho c\u00e1c ion c\u00f3 c\u00f9ng m\u1ed9t s\u1ed1 kh\u1ed1i l\u01b0\u1ee3ng c\u01a1 b\u1ea3n. Ngo\u00e0i ra, ch\u00fang cho ph\u00e9p b\u1ea1n l\u00e0m vi\u1ec7c ra trang \u0111i\u1ec3m c\u1ee7a c\u00e1c y\u1ebfu t\u1ed1. \u0110i\u1ec1u n\u00e0y ch\u1ee9ng minh r\u1ea5t h\u1eefu \u00edch trong c\u00e1c m\u1eabu \u0111\u1ea7y \u0111\u1ee7 tr\u00f4ng gi\u1ed1ng nhau ho\u1eb7c khi t\u00ecm ki\u1ebfm m\u00e0 kh\u00f4ng c\u00f3 danh s\u00e1ch c\u00e1c h\u1ee3p ch\u1ea5t \u0111\u01b0\u1ee3c thi\u1ebft l\u1eadp. Ngo\u00e0i ra, c\u00e1c h\u1ec7 th\u1ed1ng n\u00e0y cung c\u1ea5p \u0111\u1ed9 ch\u00ednh x\u00e1c kh\u1ed1i l\u01b0\u1ee3ng t\u1ed1t h\u01a1n. \u0110i\u1ec1u \u0111\u00f3 l\u00e0m t\u0103ng s\u1ef1 ch\u1eafc ch\u1eafn trong vi\u1ec7c li\u00ean k\u1ebft v\u1edbi c\u00e1c c\u00f4ng th\u1ee9c ph\u00e2n t\u1eed. C\u00e1c t\u00ednh n\u0103ng nh\u01b0 v\u1eady r\u1ea5t quan tr\u1ecdng trong c\u00e1c l\u0129nh v\u1ef1c nh\u01b0 theo d\u00f5i m\u00f4i tr\u01b0\u1eddng ho\u1eb7c ki\u1ec3m tra ch\u1ea5t l\u01b0\u1ee3ng thu\u1ed1c. N\u00f3i t\u00f3m l\u1ea1i, \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao t\u0103ng c\u01b0\u1eddng tr\u00f2 ch\u01a1i cho c\u00e1c ph\u00e2n t\u00edch kh\u00f3 kh\u0103n.<\/p>\n
Nh\u1eefng h\u1ea1n ch\u1ebf c\u1ee7a vi\u1ec7c s\u1eed d\u1ee5ng MS \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao l\u00e0 g\u00ec?<\/strong><\/h3>\nNgay c\u1ea3 v\u1edbi \u0111i\u1ec3m m\u1ea1nh c\u1ee7a h\u1ecd, c\u00e1c thi\u1ebft l\u1eadp \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao chi ph\u00ed nhi\u1ec1u h\u01a1n v\u00e0 m\u1ea5t nhi\u1ec1u n\u1ed7 l\u1ef1c \u0111\u1ec3 ch\u1ea1y h\u01a1n nh\u1eefng thi\u1ebft l\u1eadp c\u01a1 b\u1ea3n. H\u1ecd c\u1ea7n c\u00e1c b\u01b0\u1edbc \u0111i\u1ec1u ch\u1ec9nh \u0111\u1eb7c bi\u1ec7t \u0111\u1ec3 ti\u1ebfp t\u1ee5c ho\u1ea1t \u0111\u1ed9ng t\u1ed1t. Ngo\u00e0i ra, h\u1ecd ph\u1ee5 thu\u1ed9c v\u00e0o ph\u1ea7n m\u1ec1m th\u00f4ng minh \u0111\u1ec3 hi\u1ec3u \u00fd ngh\u0129a c\u1ee7a d\u1eef li\u1ec7u. C\u00e1c \u0111\u01a1n v\u1ecb nh\u01b0 Orbitraps ho\u1eb7c FT-ICR y\u00eau c\u1ea7u ki\u1ec3m so\u00e1t ch\u1eb7t ch\u1ebd m\u00f4i tr\u01b0\u1eddng xung quanh v\u00e0 b\u1ea3o tr\u00ec \u1ed5n \u0111\u1ecbnh. \u0110i\u1ec1u g\u00ec’ H\u01a1n n\u1eefa, l\u01b0\u1ee3ng d\u1eef li\u1ec7u kh\u1ed5ng l\u1ed3 t\u1eeb c\u00e1c ch\u1ea1y n\u00e0y \u0111\u00f2i h\u1ecfi c\u00e1c m\u00e1y t\u00ednh m\u1ea1nh m\u1ebd \u0111\u1ec3 x\u1eed l\u00fd t\u1ea5t c\u1ea3. V\u00ec v\u1eady, trong khi m\u1ea1nh m\u1ebd, h\u1ecd \u0111\u00f2i h\u1ecfi nhi\u1ec1u ngu\u1ed3n l\u1ef1c h\u01a1n t\u1ed5ng th\u1ec3.<\/p>\n
C\u00e1c \u1ee9ng d\u1ee5ng y\u00eau c\u1ea7u ph\u1ed5 \u0111o kh\u1ed1i l\u01b0\u1ee3ng \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao<\/strong><\/h2>\n <\/p>\n
<\/div>\nTrong c\u00e1c m\u00f4i tr\u01b0\u1eddng kh\u00f3 kh\u0103n nh\u01b0 c\u00e1c m\u1eabu m\u00f4i tr\u01b0\u1eddng ho\u1eb7c cu\u1ed9c s\u1ed1ng v\u1edbi nhi\u1ec1u h\u1ee3p ch\u1ea5t tr\u1ed9n v\u1edbi nhau, HRMS n\u1ed5i b\u1eadt \u0111\u1ec3 t\u00ecm nh\u1eefng \u0111i\u1ec1u ch\u01b0a bi\u1ebft m\u00e0 kh\u00f4ng ch\u1ec9 ph\u00f9 h\u1ee3p v\u1edbi danh s\u00e1ch. M\u1ed9t TOFMS chi ti\u1ebft c\u00f3 th\u1ec3 t\u1ef1 ph\u00e1t hi\u1ec7n c\u00e1c m\u1ee5c kh\u00f4ng bi\u1ebft, m\u00e0 kh\u00f4ng c\u1ea7n s\u1ef1 gi\u00fap \u0111\u1ee1 c\u1ee7a th\u01b0 vi\u1ec7n. N\u00f3 ph\u00f9 h\u1ee3p v\u1edbi c\u00e1c t\u00ecm ki\u1ebfm r\u1ed9ng r\u00e3i. C\u00e1c m\u1ea3nh kh\u1ed1i l\u01b0\u1ee3ng ch\u00ednh x\u00e1c v\u00e0 c\u00e1c d\u1ea5u hi\u1ec7u \u0111\u1ed3ng v\u1ecb th\u00eam v\u00e0o vi\u1ec7c t\u00ecm ra c\u1ea5u tr\u00fac. C\u00e1ch ti\u1ebfp c\u1eadn n\u00e0y t\u1ecfa s\u00e1ng b\u1edfi v\u00ec n\u00f3 x\u1eed l\u00fd s\u1ef1 ph\u1ee9c t\u1ea1p tr\u1ef1c ti\u1ebfp. V\u00ed d\u1ee5, trong c\u00e1c m\u1eabu \u0111\u00f4ng \u0111\u00fac, n\u00f3 k\u00e9o ra c\u00e1c chi ti\u1ebft m\u00e0 c\u00e1c c\u00f4ng c\u1ee5 \u0111\u01a1n gi\u1ea3n h\u01a1n b\u1ecf l\u1ee1. Do \u0111\u00f3, n\u00f3 h\u1ed7 tr\u1ee3 c\u00e1i nh\u00ecn s\u00e2u s\u1eafc h\u01a1n v\u1ec1 nh\u1eefng g\u00ec’ S hi\u1ec7n t\u1ea1i.<\/p>\n
HRMS c\u1ea3i thi\u1ec7n quy tr\u00ecnh l\u00e0m vi\u1ec7c \u0111\u1ecbnh l\u01b0\u1ee3ng nh\u01b0 th\u1ebf n\u00e0o?<\/strong><\/h3>\n\u0110\u1ec3 \u0111\u1ebfm c\u00e1c nhi\u1ec7m v\u1ee5 trong c\u00e1c l\u0129nh v\u1ef1c nh\u01b0 chuy\u1ec3n h\u00f3a ho\u1eb7c proteomics, HRMS gi\u00fap v\u1edbi s\u1ee9c m\u1ea1nh ch\u1ecdn h\u00e0ng \u0111\u1ea7u c\u1ee7a n\u00f3. N\u00f3 cho ph\u00e9p ng\u01b0\u1eddi lao \u0111\u1ed9ng ch\u1ec9 ra c\u00e1c m\u1ee5c ti\u00eau gi\u1eefa c\u00e1c c\u1ea5u tr\u00fac t\u01b0\u01a1ng t\u1ef1 ho\u1eb7c s\u1ef1 l\u1ed9n x\u1ed9n n\u1ec1n. SRM l\u00e0m cho vi\u1ec7c \u0111\u1ebfm nh\u1ea1y c\u1ea3m v\u00e0 \u1ed5n \u0111\u1ecbnh c\u00f3 th\u1ec3, ngay c\u1ea3 trong c\u00e1c m\u1eabu \u0111\u01b0\u1ee3c t\u1ea3i v\u1edbi c\u00e1c ph\u1ee5 ki\u1ec7n n\u01a1i m\u1ee5c ti\u00eau m \/ z ph\u00f9 h\u1ee3p v\u1edbi c\u00e1c ch\u1ea5t can thi\u1ec7p. Nh\u01b0 v\u1eady, HRMS ph\u1ee5c v\u1ee5 t\u1ed1t trong c\u00e1c th\u1eed nghi\u1ec7m cho d\u1ea5u v\u1ebft nh\u1ecf c\u1ee7a c\u00e1c v\u1eadt ch\u1ea5t kh\u00f4ng mong mu\u1ed1n ho\u1eb7c d\u01b0 th\u1eeba. N\u00f3 \u0111\u1ea3m b\u1ea3o c\u00e1c con s\u1ed1 \u0111\u00e1ng tin c\u1eady trong c\u00f4ng vi\u1ec7c \u0111\u01b0\u1ee3c quy \u0111\u1ecbnh.<\/p>\n
K\u1ef9 thu\u1eadt \u0111\u1ec3 t\u1ed1i \u01b0u h\u00f3a ho\u1eb7c c\u1ea3i thi\u1ec7n hi\u1ec7u su\u1ea5t \u0111\u1ed9 ph\u00e2n gi\u1ea3i MS<\/strong><\/h2>\n\u0110i\u1ec1u ch\u1ec9nh \u0111\u01b0\u1eddng d\u1eabn ion, v\u1edbi ki\u1ec3m so\u00e1t ch\u00ednh x\u00e1c c\u1ee7a \u0111i\u1ec7n \u00e1p tr\u00ean \u1ed1ng k\u00ednh \u0111i\u1ec1u khi\u1ec3n ion, c\u1eaft gi\u1ea3m s\u1ef1 ph\u00e2n t\u00e1n v\u00e0 s\u1eafc n\u00e9t tr\u1ecdng t\u00e2m. Ngo\u00e0i ra, c\u00e1c thi\u1ebft l\u1eadp trong m\u00e1y ph\u00e2n t\u00edch, nh\u01b0 \u0111\u01b0\u1eddng d\u00e0i h\u01a1n trong TOF-MS ho\u1eb7c c\u00e1c tr\u01b0\u1eddng m\u1ea1nh h\u01a1n trong FT-ICR, n\u00e2ng \u0111\u1ed9 ph\u00e2n gi\u1ea3i r\u00f5 r\u00e0ng. M\u00e1y ph\u00e2n t\u00edch t\u1ee9 c\u1ef1c v\u1edbi c\u00e1c b\u1ed9 ph\u1eadn l\u1ecdc ph\u00eda tr\u01b0\u1edbc c\u00f3 th\u1ec3 gi\u1ea3m s\u1ef1 t\u00edch t\u1ee5 b\u1ee5i b\u1ea9n. \u0110i\u1ec1u n\u00e0y t\u0103ng c\u01b0\u1eddng ch\u1ea1y \u1ed5n \u0111\u1ecbnh v\u00e0 \u00edt th\u1eddi gian d\u1eebng l\u1ea1i h\u01a1n. C\u00e1c t\u00ednh n\u0103ng n\u00e0y xu\u1ea5t hi\u1ec7n trong c\u00e1c c\u00f4ng c\u1ee5 nh\u01b0 M7 t\u1ee9 c\u1ef1c \u0111\u01a1n GC-MS<\/u><\/strong><\/a>. N\u00f3 c\u00f3 b\u1ed9 l\u1ecdc tr\u01b0\u1edbc b\u1ed1n c\u1ef1c k\u00e9o ra gi\u1eef cho ph\u1ea7n ch\u00ednh s\u1ea1ch s\u1ebd v\u00e0 b\u1ecf qua vi\u1ec7c l\u00e0m s\u1ea1ch th\u01b0\u1eddng xuy\u00ean.<\/p>\nL\u00e0m th\u1ebf n\u00e0o c\u00e1c c\u00f4ng c\u1ee5 ph\u1ea7n m\u1ec1m c\u00f3 th\u1ec3 c\u1ea3i thi\u1ec7n \u0111\u1ed9 ph\u00e2n gi\u1ea3i sau khi mua l\u1ea1i?<\/strong><\/h3>\nC\u00e1c ph\u00e2n chia \u0111\u1ec9nh d\u1ef1a tr\u00ean to\u00e1n h\u1ecdc gi\u1ea3i quy\u1ebft c\u00e1c \u0111\u1ec9nh pha tr\u1ed9n b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng c\u00e1c h\u00ecnh d\u1ea1ng v\u00e0 ph\u1ed5 bi\u1ebfn \u0111\u1ed3ng v\u1ecb d\u1ef1 ki\u1ebfn \u200b\u200btrong d\u1eef li\u1ec7u. C\u00e1c ph\u01b0\u01a1ng ph\u00e1p \u0111\u1ec3 c\u1eaft gi\u1ea3m ti\u1ebfng \u1ed3n, ch\u1eb3ng h\u1ea1n nh\u01b0 l\u00e0m m\u1ecbn k\u1ef9 thu\u1eadt s\u1ed1 v\u00e0 lo\u1ea1i b\u1ecf s\u1ef1 tr\u00f4i d\u1ea1t c\u01a1 b\u1ea3n, c\u0169ng l\u00e0m r\u00f5 c\u00e1c t\u00edn hi\u1ec7u. H\u1ecd t\u0103ng c\u01b0\u1eddng s\u1ef1 r\u00f5 r\u00e0ng gi\u00e1n ti\u1ebfp, h\u1ed7 tr\u1ee3 gi\u1ea3i quy\u1ebft. Th\u00f4ng th\u01b0\u1eddng, c\u00e1c c\u00f4ng c\u1ee5 n\u00e0y n\u1eb1m trong c\u00e1c b\u1ed9 ph\u1ea7n m\u1ec1m \u0111\u1ea7y \u0111\u1ee7 \u0111\u1ec3 gi\u00e1m s\u00e1t tr\u1ef1c ti\u1ebfp. V\u00ed d\u1ee5: G5 GC<\/u><\/strong><\/a> \u0111i k\u00e8m v\u1edbi ph\u1ea7n m\u1ec1m th\u00f4ng minh ch\u1ed1ng l\u1ea1i c\u00e1c v\u1ea5n \u0111\u1ec1 ki\u1ec3m so\u00e1t. N\u00f3 x\u1eed l\u00fd k\u1ebf ho\u1ea1ch nhi\u1ec7t \u0111\u1ed9, \u0111\u1ed3ng h\u1ed3 d\u00f2ng ch\u1ea3y v\u00e0 h\u1ed7 tr\u1ee3 m\u1ed9t s\u1ed1 m\u00e1y d\u00f2. Thi\u1ebft l\u1eadp n\u00e0y l\u00e0m cho vi\u1ec7c x\u1eed l\u00fd tr\u01a1n tru h\u01a1n v\u00e0 hi\u1ec7u qu\u1ea3 h\u01a1n.<\/p>\nPERSEE: M\u1ed9t \u0111\u1ed1i t\u00e1c \u0111\u00e1ng tin c\u1eady trong c\u00e1c gi\u1ea3i ph\u00e1p thi\u1ebft b\u1ecb ph\u00e2n t\u00edch<\/strong><\/h2>\nPERSEE<\/strong><\/a> B\u1eaft \u0111\u1ea7u v\u00e0o n\u0103m 1991 nh\u01b0 m\u1ed9t c\u00f4ng ty c\u00f4ng ngh\u1ec7 cao m\u1edbi<\/strong><\/a>N\u00f3 pha tr\u1ed9n nghi\u00ean c\u1ee9u, x\u00e2y d\u1ef1ng v\u00e0 b\u00e1n c\u00e1c c\u00f4ng c\u1ee5 khoa h\u1ecdc. V\u1edbi nhi\u1ec1u n\u0103m ki\u1ebfn th\u1ee9c trong c\u00e1c l\u0129nh v\u1ef1c nh\u01b0 quang ph\u1ed5 ph\u00e2n t\u1eed, nhi\u1ec5m s\u1eafc h\u1ecdc, quang ph\u1ed5 nguy\u00ean t\u1eed v\u00e0 h\u01a1n th\u1ebf n\u1eefa, n\u00f3 cung c\u1ea5p c\u00e2u tr\u1ea3 l\u1eddi \u0111\u1ea7y \u0111\u1ee7 cho c\u00e1c l\u0129nh v\u1ef1c nh\u01b0 d\u01b0\u1ee3c ph\u1ea9m, ki\u1ec3m tra m\u00f4i tr\u01b0\u1eddng, h\u00f3a d\u1ea7u, n\u00f4ng nghi\u1ec7p v\u00e0 gi\u1ea3ng d\u1ea1y.<\/p>\n\u0110i\u1ec1u l\u00e0m cho PERSEE \u0111\u1eb7c bi\u1ec7t l\u00e0 t\u1eadp trung v\u00e0o vi\u1ec7c t\u1ea1o ra c\u00e1c c\u00f4ng c\u1ee5 ch\u00ednh x\u00e1c v\u1edbi s\u1ef1 gi\u00fap \u0111\u1ee1 tr\u00ean to\u00e0n th\u1ebf gi\u1edbi. C\u00e1c \u00fd t\u01b0\u1edfng m\u1edbi c\u1ee7a n\u00f3, t\u1eeb m\u00e1y quang ph\u1ed5 UV-Vis \u0111\u1ebfn m\u00e1y nhi\u1ec5m s\u1eafc kh\u00ed chi ti\u1ebft, cho th\u1ea5y m\u1ed9t c\u00e1i nh\u00ecn th\u00f4ng minh. H\u1ecd gi\u1ea3i quy\u1ebft c\u00e1c v\u1ea5n \u0111\u1ec1 hi\u1ec7n t\u1ea1i trong ph\u00e2n t\u00edch v\u00e0 l\u1eadp k\u1ebf ho\u1ea1ch cho nh\u1eefng g\u00ec’ S ti\u1ebfp theo. M\u1ecdi ng\u01b0\u1eddi tr\u00ean to\u00e0n c\u1ea7u d\u1ef1a v\u00e0o c\u00e1c s\u1ea3n ph\u1ea9m PERSEE cho k\u1ebft qu\u1ea3 \u0111\u00fang \u0111\u1eafn, x\u00e2y d\u1ef1ng kh\u00f3 kh\u0103n v\u00e0 d\u1ec5 s\u1eed d\u1ee5ng.<\/p>\n
Nh\u1eadn x\u00e9t t\u00f3m t\u1eaft v\u1ec1 \u0111\u1ecbnh ngh\u0129a v\u00e0 \u00e1p d\u1ee5ng Ngh\u1ecb quy\u1ebft HRMS<\/strong><\/h2>\n\u0110\u1ed9 ph\u00e2n gi\u1ea3i l\u00e0 trung t\u00e2m c\u1ee7a c\u00f4ng ngh\u1ec7 HRMS. N\u00f3 mang l\u1ea1i \u0111\u1ed9 ch\u00ednh x\u00e1c h\u00e0ng \u0111\u1ea7u \u0111\u1ec3 ph\u00e1t hi\u1ec7n v\u00e0 \u0111\u1ebfm c\u00e1c nhi\u1ec7m v\u1ee5. C\u00e1c b\u1ed9 ph\u1eadn c\u1ee7a thi\u1ebft b\u1ecb, nh\u01b0 phong c\u00e1ch ph\u00e2n t\u00edch, thi\u1ebft l\u1eadp \u0111\u01b0\u1eddng d\u1eabn ion v\u00e0 b\u1ed9 b\u1eaft t\u00edn hi\u1ec7u, x\u00e2y d\u1ef1ng c\u01a1 s\u1edf cho vi\u1ec7c n\u00f3 gi\u1ea3i quy\u1ebft t\u1ed1t nh\u01b0 th\u1ebf n\u00e0o. C\u00e0i \u0111\u1eb7t trong khi ch\u1ea1y, ch\u1eb3ng h\u1ea1n nh\u01b0 t\u1ed1c \u0111\u1ed9 qu\u00e9t v\u00e0 \u0111i\u1ec1u ki\u1ec7n ngu\u1ed3n, c\u1ea7n ph\u00f9 h\u1ee3p \u0111\u1ec3 t\u1eadn d\u1ee5ng t\u1ed1i \u0111a. Cu\u1ed1i c\u00f9ng, \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao nh\u1ea5t \u0111\u1ebfn t\u1eeb vi\u1ec7c pha tr\u1ed9n ch\u0103m s\u00f3c ph\u1ea7n c\u1ee9ng v\u1edbi c\u00e1c b\u01b0\u1edbc ph\u1ea7n m\u1ec1m th\u00f4ng minh. H\u1ed7n h\u1ee3p n\u00e0y m\u1edf r\u1ed9ng nh\u1eefng g\u00ec quang ph\u1ed5 kh\u1ed1i l\u01b0\u1ee3ng c\u00f3 th\u1ec3 l\u00e0m trong c\u00e1c khu v\u1ef1c c\u1ea7n k\u1ebft qu\u1ea3 ch\u1eafc ch\u1eafn \u1edf m\u1ee9c th\u1ea5p.<\/p>\n
C\u00e2u h\u1ecfi th\u01b0\u1eddng g\u1eb7p<\/strong><\/h2>\nQ1: Gi\u00e1 tr\u1ecb \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao trong ph\u1ed5 \u0111o kh\u1ed1i l\u01b0\u1ee3ng \u0111\u01b0\u1ee3c coi l\u00e0 g\u00ec?
\n<\/strong>A1: Th\u00f4ng th\u01b0\u1eddng, c\u00f4ng su\u1ea5t gi\u1ea3i quy\u1ebft l\u1edbn h\u01a1n 10.000 (m \/ \u0394m t\u1ea1i FWHM) \u0111\u01b0\u1ee3c coi l\u00e0 \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao; M\u1ed9t s\u1ed1 thi\u1ebft b\u1ecb v\u01b0\u1ee3t qu\u00e1 100.000 t\u00f9y thu\u1ed9c v\u00e0o lo\u1ea1i m\u00e1y ph\u00e2n t\u00edch.<\/p>\nQ2: Ph\u1ed5 \u0111o kh\u1ed1i l\u01b0\u1ee3ng \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng cho ph\u00e2n t\u00edch \u0111\u1ecbnh l\u01b0\u1ee3ng th\u01b0\u1eddng xuy\u00ean kh\u00f4ng?
\n<\/strong>A2: V\u00e2ng, HRMS cung c\u1ea5p t\u00ednh ch\u1ecdn l\u1ecdc tuy\u1ec7t v\u1eddi n\u00e2ng cao \u0111\u1ed9 ch\u00ednh x\u00e1c \u0111\u1ecbnh l\u01b0\u1ee3ng ngay c\u1ea3 trong ma tr\u1eadn ph\u1ee9c t\u1ea1p; Tuy nhi\u00ean, vi\u1ec7c ph\u00e1t tri\u1ec3n ph\u01b0\u01a1ng ph\u00e1p ph\u1ea3i t\u00ednh \u0111\u1ebfn c\u00e1c th\u00f4ng s\u1ed1 c\u1ee5 th\u1ec3 c\u1ee7a d\u1ee5ng c\u1ee5.<\/p>\nQ3: M\u1ed9t d\u1ee5ng c\u1ee5 \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao n\u00ean \u0111\u01b0\u1ee3c hi\u1ec7u chu\u1ea9n bao l\u00e2u?
\n<\/strong>A3: T\u1ea7n s\u1ed1 hi\u1ec7u chu\u1ea9n ph\u1ee5 thu\u1ed9c v\u00e0o c\u01b0\u1eddng \u0111\u1ed9 s\u1eed d\u1ee5ng nh\u01b0ng n\u00f3i chung n\u00ean \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n h\u00e0ng ng\u00e0y ho\u1eb7c tr\u01b0\u1edbc m\u1ed7i l\u00f4 ph\u00e2n t\u00edch \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o vi\u1ec7c g\u00e1n kh\u1ed1i l\u01b0\u1ee3ng ch\u00ednh x\u00e1c v\u00e0 hi\u1ec7u su\u1ea5t nh\u1ea5t qu\u00e1n.<\/p>","protected":false},"excerpt":{"rendered":" In high resolution mass spectrometry (HRMS), resolution means the device’s skill in telling apart ions with almost the same mass-to-charge (m\/z) values. This skill matters a lot for spotting compounds correctly, particularly in tricky mixtures. In math terms, resolution shows as R = m\/\u0394m. Here, “m” stands for the m\/z at the main peak, […]<\/p>\n","protected":false},"author":1,"featured_media":4261,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[38,37],"tags":[],"class_list":["post-4265","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-industry-information","category-news"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts\/4265","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/comments?post=4265"}],"version-history":[{"count":4,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts\/4265\/revisions"}],"predecessor-version":[{"id":4273,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts\/4265\/revisions\/4273"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/media\/4261"}],"wp:attachment":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/media?parent=4265"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/categories?post=4265"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/tags?post=4265"}],"curies":[{"name":"WP","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
<\/p>\n
<\/div>\nTrong c\u00e1c m\u00f4i tr\u01b0\u1eddng kh\u00f3 kh\u0103n nh\u01b0 c\u00e1c m\u1eabu m\u00f4i tr\u01b0\u1eddng ho\u1eb7c cu\u1ed9c s\u1ed1ng v\u1edbi nhi\u1ec1u h\u1ee3p ch\u1ea5t tr\u1ed9n v\u1edbi nhau, HRMS n\u1ed5i b\u1eadt \u0111\u1ec3 t\u00ecm nh\u1eefng \u0111i\u1ec1u ch\u01b0a bi\u1ebft m\u00e0 kh\u00f4ng ch\u1ec9 ph\u00f9 h\u1ee3p v\u1edbi danh s\u00e1ch. M\u1ed9t TOFMS chi ti\u1ebft c\u00f3 th\u1ec3 t\u1ef1 ph\u00e1t hi\u1ec7n c\u00e1c m\u1ee5c kh\u00f4ng bi\u1ebft, m\u00e0 kh\u00f4ng c\u1ea7n s\u1ef1 gi\u00fap \u0111\u1ee1 c\u1ee7a th\u01b0 vi\u1ec7n. N\u00f3 ph\u00f9 h\u1ee3p v\u1edbi c\u00e1c t\u00ecm ki\u1ebfm r\u1ed9ng r\u00e3i. C\u00e1c m\u1ea3nh kh\u1ed1i l\u01b0\u1ee3ng ch\u00ednh x\u00e1c v\u00e0 c\u00e1c d\u1ea5u hi\u1ec7u \u0111\u1ed3ng v\u1ecb th\u00eam v\u00e0o vi\u1ec7c t\u00ecm ra c\u1ea5u tr\u00fac. C\u00e1ch ti\u1ebfp c\u1eadn n\u00e0y t\u1ecfa s\u00e1ng b\u1edfi v\u00ec n\u00f3 x\u1eed l\u00fd s\u1ef1 ph\u1ee9c t\u1ea1p tr\u1ef1c ti\u1ebfp. V\u00ed d\u1ee5, trong c\u00e1c m\u1eabu \u0111\u00f4ng \u0111\u00fac, n\u00f3 k\u00e9o ra c\u00e1c chi ti\u1ebft m\u00e0 c\u00e1c c\u00f4ng c\u1ee5 \u0111\u01a1n gi\u1ea3n h\u01a1n b\u1ecf l\u1ee1. Do \u0111\u00f3, n\u00f3 h\u1ed7 tr\u1ee3 c\u00e1i nh\u00ecn s\u00e2u s\u1eafc h\u01a1n v\u1ec1 nh\u1eefng g\u00ec’ S hi\u1ec7n t\u1ea1i.<\/p>\n
HRMS c\u1ea3i thi\u1ec7n quy tr\u00ecnh l\u00e0m vi\u1ec7c \u0111\u1ecbnh l\u01b0\u1ee3ng nh\u01b0 th\u1ebf n\u00e0o?<\/strong><\/h3>\n\u0110\u1ec3 \u0111\u1ebfm c\u00e1c nhi\u1ec7m v\u1ee5 trong c\u00e1c l\u0129nh v\u1ef1c nh\u01b0 chuy\u1ec3n h\u00f3a ho\u1eb7c proteomics, HRMS gi\u00fap v\u1edbi s\u1ee9c m\u1ea1nh ch\u1ecdn h\u00e0ng \u0111\u1ea7u c\u1ee7a n\u00f3. N\u00f3 cho ph\u00e9p ng\u01b0\u1eddi lao \u0111\u1ed9ng ch\u1ec9 ra c\u00e1c m\u1ee5c ti\u00eau gi\u1eefa c\u00e1c c\u1ea5u tr\u00fac t\u01b0\u01a1ng t\u1ef1 ho\u1eb7c s\u1ef1 l\u1ed9n x\u1ed9n n\u1ec1n. SRM l\u00e0m cho vi\u1ec7c \u0111\u1ebfm nh\u1ea1y c\u1ea3m v\u00e0 \u1ed5n \u0111\u1ecbnh c\u00f3 th\u1ec3, ngay c\u1ea3 trong c\u00e1c m\u1eabu \u0111\u01b0\u1ee3c t\u1ea3i v\u1edbi c\u00e1c ph\u1ee5 ki\u1ec7n n\u01a1i m\u1ee5c ti\u00eau m \/ z ph\u00f9 h\u1ee3p v\u1edbi c\u00e1c ch\u1ea5t can thi\u1ec7p. Nh\u01b0 v\u1eady, HRMS ph\u1ee5c v\u1ee5 t\u1ed1t trong c\u00e1c th\u1eed nghi\u1ec7m cho d\u1ea5u v\u1ebft nh\u1ecf c\u1ee7a c\u00e1c v\u1eadt ch\u1ea5t kh\u00f4ng mong mu\u1ed1n ho\u1eb7c d\u01b0 th\u1eeba. N\u00f3 \u0111\u1ea3m b\u1ea3o c\u00e1c con s\u1ed1 \u0111\u00e1ng tin c\u1eady trong c\u00f4ng vi\u1ec7c \u0111\u01b0\u1ee3c quy \u0111\u1ecbnh.<\/p>\n
K\u1ef9 thu\u1eadt \u0111\u1ec3 t\u1ed1i \u01b0u h\u00f3a ho\u1eb7c c\u1ea3i thi\u1ec7n hi\u1ec7u su\u1ea5t \u0111\u1ed9 ph\u00e2n gi\u1ea3i MS<\/strong><\/h2>\n\u0110i\u1ec1u ch\u1ec9nh \u0111\u01b0\u1eddng d\u1eabn ion, v\u1edbi ki\u1ec3m so\u00e1t ch\u00ednh x\u00e1c c\u1ee7a \u0111i\u1ec7n \u00e1p tr\u00ean \u1ed1ng k\u00ednh \u0111i\u1ec1u khi\u1ec3n ion, c\u1eaft gi\u1ea3m s\u1ef1 ph\u00e2n t\u00e1n v\u00e0 s\u1eafc n\u00e9t tr\u1ecdng t\u00e2m. Ngo\u00e0i ra, c\u00e1c thi\u1ebft l\u1eadp trong m\u00e1y ph\u00e2n t\u00edch, nh\u01b0 \u0111\u01b0\u1eddng d\u00e0i h\u01a1n trong TOF-MS ho\u1eb7c c\u00e1c tr\u01b0\u1eddng m\u1ea1nh h\u01a1n trong FT-ICR, n\u00e2ng \u0111\u1ed9 ph\u00e2n gi\u1ea3i r\u00f5 r\u00e0ng. M\u00e1y ph\u00e2n t\u00edch t\u1ee9 c\u1ef1c v\u1edbi c\u00e1c b\u1ed9 ph\u1eadn l\u1ecdc ph\u00eda tr\u01b0\u1edbc c\u00f3 th\u1ec3 gi\u1ea3m s\u1ef1 t\u00edch t\u1ee5 b\u1ee5i b\u1ea9n. \u0110i\u1ec1u n\u00e0y t\u0103ng c\u01b0\u1eddng ch\u1ea1y \u1ed5n \u0111\u1ecbnh v\u00e0 \u00edt th\u1eddi gian d\u1eebng l\u1ea1i h\u01a1n. C\u00e1c t\u00ednh n\u0103ng n\u00e0y xu\u1ea5t hi\u1ec7n trong c\u00e1c c\u00f4ng c\u1ee5 nh\u01b0 M7 t\u1ee9 c\u1ef1c \u0111\u01a1n GC-MS<\/u><\/strong><\/a>. N\u00f3 c\u00f3 b\u1ed9 l\u1ecdc tr\u01b0\u1edbc b\u1ed1n c\u1ef1c k\u00e9o ra gi\u1eef cho ph\u1ea7n ch\u00ednh s\u1ea1ch s\u1ebd v\u00e0 b\u1ecf qua vi\u1ec7c l\u00e0m s\u1ea1ch th\u01b0\u1eddng xuy\u00ean.<\/p>\nL\u00e0m th\u1ebf n\u00e0o c\u00e1c c\u00f4ng c\u1ee5 ph\u1ea7n m\u1ec1m c\u00f3 th\u1ec3 c\u1ea3i thi\u1ec7n \u0111\u1ed9 ph\u00e2n gi\u1ea3i sau khi mua l\u1ea1i?<\/strong><\/h3>\nC\u00e1c ph\u00e2n chia \u0111\u1ec9nh d\u1ef1a tr\u00ean to\u00e1n h\u1ecdc gi\u1ea3i quy\u1ebft c\u00e1c \u0111\u1ec9nh pha tr\u1ed9n b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng c\u00e1c h\u00ecnh d\u1ea1ng v\u00e0 ph\u1ed5 bi\u1ebfn \u0111\u1ed3ng v\u1ecb d\u1ef1 ki\u1ebfn \u200b\u200btrong d\u1eef li\u1ec7u. C\u00e1c ph\u01b0\u01a1ng ph\u00e1p \u0111\u1ec3 c\u1eaft gi\u1ea3m ti\u1ebfng \u1ed3n, ch\u1eb3ng h\u1ea1n nh\u01b0 l\u00e0m m\u1ecbn k\u1ef9 thu\u1eadt s\u1ed1 v\u00e0 lo\u1ea1i b\u1ecf s\u1ef1 tr\u00f4i d\u1ea1t c\u01a1 b\u1ea3n, c\u0169ng l\u00e0m r\u00f5 c\u00e1c t\u00edn hi\u1ec7u. H\u1ecd t\u0103ng c\u01b0\u1eddng s\u1ef1 r\u00f5 r\u00e0ng gi\u00e1n ti\u1ebfp, h\u1ed7 tr\u1ee3 gi\u1ea3i quy\u1ebft. Th\u00f4ng th\u01b0\u1eddng, c\u00e1c c\u00f4ng c\u1ee5 n\u00e0y n\u1eb1m trong c\u00e1c b\u1ed9 ph\u1ea7n m\u1ec1m \u0111\u1ea7y \u0111\u1ee7 \u0111\u1ec3 gi\u00e1m s\u00e1t tr\u1ef1c ti\u1ebfp. V\u00ed d\u1ee5: G5 GC<\/u><\/strong><\/a> \u0111i k\u00e8m v\u1edbi ph\u1ea7n m\u1ec1m th\u00f4ng minh ch\u1ed1ng l\u1ea1i c\u00e1c v\u1ea5n \u0111\u1ec1 ki\u1ec3m so\u00e1t. N\u00f3 x\u1eed l\u00fd k\u1ebf ho\u1ea1ch nhi\u1ec7t \u0111\u1ed9, \u0111\u1ed3ng h\u1ed3 d\u00f2ng ch\u1ea3y v\u00e0 h\u1ed7 tr\u1ee3 m\u1ed9t s\u1ed1 m\u00e1y d\u00f2. Thi\u1ebft l\u1eadp n\u00e0y l\u00e0m cho vi\u1ec7c x\u1eed l\u00fd tr\u01a1n tru h\u01a1n v\u00e0 hi\u1ec7u qu\u1ea3 h\u01a1n.<\/p>\nPERSEE: M\u1ed9t \u0111\u1ed1i t\u00e1c \u0111\u00e1ng tin c\u1eady trong c\u00e1c gi\u1ea3i ph\u00e1p thi\u1ebft b\u1ecb ph\u00e2n t\u00edch<\/strong><\/h2>\nPERSEE<\/strong><\/a> B\u1eaft \u0111\u1ea7u v\u00e0o n\u0103m 1991 nh\u01b0 m\u1ed9t c\u00f4ng ty c\u00f4ng ngh\u1ec7 cao m\u1edbi<\/strong><\/a>N\u00f3 pha tr\u1ed9n nghi\u00ean c\u1ee9u, x\u00e2y d\u1ef1ng v\u00e0 b\u00e1n c\u00e1c c\u00f4ng c\u1ee5 khoa h\u1ecdc. V\u1edbi nhi\u1ec1u n\u0103m ki\u1ebfn th\u1ee9c trong c\u00e1c l\u0129nh v\u1ef1c nh\u01b0 quang ph\u1ed5 ph\u00e2n t\u1eed, nhi\u1ec5m s\u1eafc h\u1ecdc, quang ph\u1ed5 nguy\u00ean t\u1eed v\u00e0 h\u01a1n th\u1ebf n\u1eefa, n\u00f3 cung c\u1ea5p c\u00e2u tr\u1ea3 l\u1eddi \u0111\u1ea7y \u0111\u1ee7 cho c\u00e1c l\u0129nh v\u1ef1c nh\u01b0 d\u01b0\u1ee3c ph\u1ea9m, ki\u1ec3m tra m\u00f4i tr\u01b0\u1eddng, h\u00f3a d\u1ea7u, n\u00f4ng nghi\u1ec7p v\u00e0 gi\u1ea3ng d\u1ea1y.<\/p>\n\u0110i\u1ec1u l\u00e0m cho PERSEE \u0111\u1eb7c bi\u1ec7t l\u00e0 t\u1eadp trung v\u00e0o vi\u1ec7c t\u1ea1o ra c\u00e1c c\u00f4ng c\u1ee5 ch\u00ednh x\u00e1c v\u1edbi s\u1ef1 gi\u00fap \u0111\u1ee1 tr\u00ean to\u00e0n th\u1ebf gi\u1edbi. C\u00e1c \u00fd t\u01b0\u1edfng m\u1edbi c\u1ee7a n\u00f3, t\u1eeb m\u00e1y quang ph\u1ed5 UV-Vis \u0111\u1ebfn m\u00e1y nhi\u1ec5m s\u1eafc kh\u00ed chi ti\u1ebft, cho th\u1ea5y m\u1ed9t c\u00e1i nh\u00ecn th\u00f4ng minh. H\u1ecd gi\u1ea3i quy\u1ebft c\u00e1c v\u1ea5n \u0111\u1ec1 hi\u1ec7n t\u1ea1i trong ph\u00e2n t\u00edch v\u00e0 l\u1eadp k\u1ebf ho\u1ea1ch cho nh\u1eefng g\u00ec’ S ti\u1ebfp theo. M\u1ecdi ng\u01b0\u1eddi tr\u00ean to\u00e0n c\u1ea7u d\u1ef1a v\u00e0o c\u00e1c s\u1ea3n ph\u1ea9m PERSEE cho k\u1ebft qu\u1ea3 \u0111\u00fang \u0111\u1eafn, x\u00e2y d\u1ef1ng kh\u00f3 kh\u0103n v\u00e0 d\u1ec5 s\u1eed d\u1ee5ng.<\/p>\n
Nh\u1eadn x\u00e9t t\u00f3m t\u1eaft v\u1ec1 \u0111\u1ecbnh ngh\u0129a v\u00e0 \u00e1p d\u1ee5ng Ngh\u1ecb quy\u1ebft HRMS<\/strong><\/h2>\n\u0110\u1ed9 ph\u00e2n gi\u1ea3i l\u00e0 trung t\u00e2m c\u1ee7a c\u00f4ng ngh\u1ec7 HRMS. N\u00f3 mang l\u1ea1i \u0111\u1ed9 ch\u00ednh x\u00e1c h\u00e0ng \u0111\u1ea7u \u0111\u1ec3 ph\u00e1t hi\u1ec7n v\u00e0 \u0111\u1ebfm c\u00e1c nhi\u1ec7m v\u1ee5. C\u00e1c b\u1ed9 ph\u1eadn c\u1ee7a thi\u1ebft b\u1ecb, nh\u01b0 phong c\u00e1ch ph\u00e2n t\u00edch, thi\u1ebft l\u1eadp \u0111\u01b0\u1eddng d\u1eabn ion v\u00e0 b\u1ed9 b\u1eaft t\u00edn hi\u1ec7u, x\u00e2y d\u1ef1ng c\u01a1 s\u1edf cho vi\u1ec7c n\u00f3 gi\u1ea3i quy\u1ebft t\u1ed1t nh\u01b0 th\u1ebf n\u00e0o. C\u00e0i \u0111\u1eb7t trong khi ch\u1ea1y, ch\u1eb3ng h\u1ea1n nh\u01b0 t\u1ed1c \u0111\u1ed9 qu\u00e9t v\u00e0 \u0111i\u1ec1u ki\u1ec7n ngu\u1ed3n, c\u1ea7n ph\u00f9 h\u1ee3p \u0111\u1ec3 t\u1eadn d\u1ee5ng t\u1ed1i \u0111a. Cu\u1ed1i c\u00f9ng, \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao nh\u1ea5t \u0111\u1ebfn t\u1eeb vi\u1ec7c pha tr\u1ed9n ch\u0103m s\u00f3c ph\u1ea7n c\u1ee9ng v\u1edbi c\u00e1c b\u01b0\u1edbc ph\u1ea7n m\u1ec1m th\u00f4ng minh. H\u1ed7n h\u1ee3p n\u00e0y m\u1edf r\u1ed9ng nh\u1eefng g\u00ec quang ph\u1ed5 kh\u1ed1i l\u01b0\u1ee3ng c\u00f3 th\u1ec3 l\u00e0m trong c\u00e1c khu v\u1ef1c c\u1ea7n k\u1ebft qu\u1ea3 ch\u1eafc ch\u1eafn \u1edf m\u1ee9c th\u1ea5p.<\/p>\n
C\u00e2u h\u1ecfi th\u01b0\u1eddng g\u1eb7p<\/strong><\/h2>\nQ1: Gi\u00e1 tr\u1ecb \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao trong ph\u1ed5 \u0111o kh\u1ed1i l\u01b0\u1ee3ng \u0111\u01b0\u1ee3c coi l\u00e0 g\u00ec?
\n<\/strong>A1: Th\u00f4ng th\u01b0\u1eddng, c\u00f4ng su\u1ea5t gi\u1ea3i quy\u1ebft l\u1edbn h\u01a1n 10.000 (m \/ \u0394m t\u1ea1i FWHM) \u0111\u01b0\u1ee3c coi l\u00e0 \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao; M\u1ed9t s\u1ed1 thi\u1ebft b\u1ecb v\u01b0\u1ee3t qu\u00e1 100.000 t\u00f9y thu\u1ed9c v\u00e0o lo\u1ea1i m\u00e1y ph\u00e2n t\u00edch.<\/p>\nQ2: Ph\u1ed5 \u0111o kh\u1ed1i l\u01b0\u1ee3ng \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng cho ph\u00e2n t\u00edch \u0111\u1ecbnh l\u01b0\u1ee3ng th\u01b0\u1eddng xuy\u00ean kh\u00f4ng?
\n<\/strong>A2: V\u00e2ng, HRMS cung c\u1ea5p t\u00ednh ch\u1ecdn l\u1ecdc tuy\u1ec7t v\u1eddi n\u00e2ng cao \u0111\u1ed9 ch\u00ednh x\u00e1c \u0111\u1ecbnh l\u01b0\u1ee3ng ngay c\u1ea3 trong ma tr\u1eadn ph\u1ee9c t\u1ea1p; Tuy nhi\u00ean, vi\u1ec7c ph\u00e1t tri\u1ec3n ph\u01b0\u01a1ng ph\u00e1p ph\u1ea3i t\u00ednh \u0111\u1ebfn c\u00e1c th\u00f4ng s\u1ed1 c\u1ee5 th\u1ec3 c\u1ee7a d\u1ee5ng c\u1ee5.<\/p>\nQ3: M\u1ed9t d\u1ee5ng c\u1ee5 \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao n\u00ean \u0111\u01b0\u1ee3c hi\u1ec7u chu\u1ea9n bao l\u00e2u?
\n<\/strong>A3: T\u1ea7n s\u1ed1 hi\u1ec7u chu\u1ea9n ph\u1ee5 thu\u1ed9c v\u00e0o c\u01b0\u1eddng \u0111\u1ed9 s\u1eed d\u1ee5ng nh\u01b0ng n\u00f3i chung n\u00ean \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n h\u00e0ng ng\u00e0y ho\u1eb7c tr\u01b0\u1edbc m\u1ed7i l\u00f4 ph\u00e2n t\u00edch \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o vi\u1ec7c g\u00e1n kh\u1ed1i l\u01b0\u1ee3ng ch\u00ednh x\u00e1c v\u00e0 hi\u1ec7u su\u1ea5t nh\u1ea5t qu\u00e1n.<\/p>","protected":false},"excerpt":{"rendered":" In high resolution mass spectrometry (HRMS), resolution means the device’s skill in telling apart ions with almost the same mass-to-charge (m\/z) values. This skill matters a lot for spotting compounds correctly, particularly in tricky mixtures. In math terms, resolution shows as R = m\/\u0394m. Here, “m” stands for the m\/z at the main peak, […]<\/p>\n","protected":false},"author":1,"featured_media":4261,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[38,37],"tags":[],"class_list":["post-4265","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-industry-information","category-news"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts\/4265","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/comments?post=4265"}],"version-history":[{"count":4,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts\/4265\/revisions"}],"predecessor-version":[{"id":4273,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts\/4265\/revisions\/4273"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/media\/4261"}],"wp:attachment":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/media?parent=4265"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/categories?post=4265"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/tags?post=4265"}],"curies":[{"name":"WP","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
\u0110i\u1ec1u ch\u1ec9nh \u0111\u01b0\u1eddng d\u1eabn ion, v\u1edbi ki\u1ec3m so\u00e1t ch\u00ednh x\u00e1c c\u1ee7a \u0111i\u1ec7n \u00e1p tr\u00ean \u1ed1ng k\u00ednh \u0111i\u1ec1u khi\u1ec3n ion, c\u1eaft gi\u1ea3m s\u1ef1 ph\u00e2n t\u00e1n v\u00e0 s\u1eafc n\u00e9t tr\u1ecdng t\u00e2m. Ngo\u00e0i ra, c\u00e1c thi\u1ebft l\u1eadp trong m\u00e1y ph\u00e2n t\u00edch, nh\u01b0 \u0111\u01b0\u1eddng d\u00e0i h\u01a1n trong TOF-MS ho\u1eb7c c\u00e1c tr\u01b0\u1eddng m\u1ea1nh h\u01a1n trong FT-ICR, n\u00e2ng \u0111\u1ed9 ph\u00e2n gi\u1ea3i r\u00f5 r\u00e0ng. M\u00e1y ph\u00e2n t\u00edch t\u1ee9 c\u1ef1c v\u1edbi c\u00e1c b\u1ed9 ph\u1eadn l\u1ecdc ph\u00eda tr\u01b0\u1edbc c\u00f3 th\u1ec3 gi\u1ea3m s\u1ef1 t\u00edch t\u1ee5 b\u1ee5i b\u1ea9n. \u0110i\u1ec1u n\u00e0y t\u0103ng c\u01b0\u1eddng ch\u1ea1y \u1ed5n \u0111\u1ecbnh v\u00e0 \u00edt th\u1eddi gian d\u1eebng l\u1ea1i h\u01a1n. C\u00e1c t\u00ednh n\u0103ng n\u00e0y xu\u1ea5t hi\u1ec7n trong c\u00e1c c\u00f4ng c\u1ee5 nh\u01b0 M7 t\u1ee9 c\u1ef1c \u0111\u01a1n GC-MS<\/u><\/strong><\/a>. N\u00f3 c\u00f3 b\u1ed9 l\u1ecdc tr\u01b0\u1edbc b\u1ed1n c\u1ef1c k\u00e9o ra gi\u1eef cho ph\u1ea7n ch\u00ednh s\u1ea1ch s\u1ebd v\u00e0 b\u1ecf qua vi\u1ec7c l\u00e0m s\u1ea1ch th\u01b0\u1eddng xuy\u00ean.<\/p>\n C\u00e1c ph\u00e2n chia \u0111\u1ec9nh d\u1ef1a tr\u00ean to\u00e1n h\u1ecdc gi\u1ea3i quy\u1ebft c\u00e1c \u0111\u1ec9nh pha tr\u1ed9n b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng c\u00e1c h\u00ecnh d\u1ea1ng v\u00e0 ph\u1ed5 bi\u1ebfn \u0111\u1ed3ng v\u1ecb d\u1ef1 ki\u1ebfn \u200b\u200btrong d\u1eef li\u1ec7u. C\u00e1c ph\u01b0\u01a1ng ph\u00e1p \u0111\u1ec3 c\u1eaft gi\u1ea3m ti\u1ebfng \u1ed3n, ch\u1eb3ng h\u1ea1n nh\u01b0 l\u00e0m m\u1ecbn k\u1ef9 thu\u1eadt s\u1ed1 v\u00e0 lo\u1ea1i b\u1ecf s\u1ef1 tr\u00f4i d\u1ea1t c\u01a1 b\u1ea3n, c\u0169ng l\u00e0m r\u00f5 c\u00e1c t\u00edn hi\u1ec7u. H\u1ecd t\u0103ng c\u01b0\u1eddng s\u1ef1 r\u00f5 r\u00e0ng gi\u00e1n ti\u1ebfp, h\u1ed7 tr\u1ee3 gi\u1ea3i quy\u1ebft. Th\u00f4ng th\u01b0\u1eddng, c\u00e1c c\u00f4ng c\u1ee5 n\u00e0y n\u1eb1m trong c\u00e1c b\u1ed9 ph\u1ea7n m\u1ec1m \u0111\u1ea7y \u0111\u1ee7 \u0111\u1ec3 gi\u00e1m s\u00e1t tr\u1ef1c ti\u1ebfp. V\u00ed d\u1ee5: G5 GC<\/u><\/strong><\/a> \u0111i k\u00e8m v\u1edbi ph\u1ea7n m\u1ec1m th\u00f4ng minh ch\u1ed1ng l\u1ea1i c\u00e1c v\u1ea5n \u0111\u1ec1 ki\u1ec3m so\u00e1t. N\u00f3 x\u1eed l\u00fd k\u1ebf ho\u1ea1ch nhi\u1ec7t \u0111\u1ed9, \u0111\u1ed3ng h\u1ed3 d\u00f2ng ch\u1ea3y v\u00e0 h\u1ed7 tr\u1ee3 m\u1ed9t s\u1ed1 m\u00e1y d\u00f2. Thi\u1ebft l\u1eadp n\u00e0y l\u00e0m cho vi\u1ec7c x\u1eed l\u00fd tr\u01a1n tru h\u01a1n v\u00e0 hi\u1ec7u qu\u1ea3 h\u01a1n.<\/p>\n PERSEE<\/strong><\/a> B\u1eaft \u0111\u1ea7u v\u00e0o n\u0103m 1991 nh\u01b0 m\u1ed9t c\u00f4ng ty c\u00f4ng ngh\u1ec7 cao m\u1edbi<\/strong><\/a>N\u00f3 pha tr\u1ed9n nghi\u00ean c\u1ee9u, x\u00e2y d\u1ef1ng v\u00e0 b\u00e1n c\u00e1c c\u00f4ng c\u1ee5 khoa h\u1ecdc. V\u1edbi nhi\u1ec1u n\u0103m ki\u1ebfn th\u1ee9c trong c\u00e1c l\u0129nh v\u1ef1c nh\u01b0 quang ph\u1ed5 ph\u00e2n t\u1eed, nhi\u1ec5m s\u1eafc h\u1ecdc, quang ph\u1ed5 nguy\u00ean t\u1eed v\u00e0 h\u01a1n th\u1ebf n\u1eefa, n\u00f3 cung c\u1ea5p c\u00e2u tr\u1ea3 l\u1eddi \u0111\u1ea7y \u0111\u1ee7 cho c\u00e1c l\u0129nh v\u1ef1c nh\u01b0 d\u01b0\u1ee3c ph\u1ea9m, ki\u1ec3m tra m\u00f4i tr\u01b0\u1eddng, h\u00f3a d\u1ea7u, n\u00f4ng nghi\u1ec7p v\u00e0 gi\u1ea3ng d\u1ea1y.<\/p>\n \u0110i\u1ec1u l\u00e0m cho PERSEE \u0111\u1eb7c bi\u1ec7t l\u00e0 t\u1eadp trung v\u00e0o vi\u1ec7c t\u1ea1o ra c\u00e1c c\u00f4ng c\u1ee5 ch\u00ednh x\u00e1c v\u1edbi s\u1ef1 gi\u00fap \u0111\u1ee1 tr\u00ean to\u00e0n th\u1ebf gi\u1edbi. C\u00e1c \u00fd t\u01b0\u1edfng m\u1edbi c\u1ee7a n\u00f3, t\u1eeb m\u00e1y quang ph\u1ed5 UV-Vis \u0111\u1ebfn m\u00e1y nhi\u1ec5m s\u1eafc kh\u00ed chi ti\u1ebft, cho th\u1ea5y m\u1ed9t c\u00e1i nh\u00ecn th\u00f4ng minh. H\u1ecd gi\u1ea3i quy\u1ebft c\u00e1c v\u1ea5n \u0111\u1ec1 hi\u1ec7n t\u1ea1i trong ph\u00e2n t\u00edch v\u00e0 l\u1eadp k\u1ebf ho\u1ea1ch cho nh\u1eefng g\u00ec’ S ti\u1ebfp theo. M\u1ecdi ng\u01b0\u1eddi tr\u00ean to\u00e0n c\u1ea7u d\u1ef1a v\u00e0o c\u00e1c s\u1ea3n ph\u1ea9m PERSEE cho k\u1ebft qu\u1ea3 \u0111\u00fang \u0111\u1eafn, x\u00e2y d\u1ef1ng kh\u00f3 kh\u0103n v\u00e0 d\u1ec5 s\u1eed d\u1ee5ng.<\/p>\n \u0110\u1ed9 ph\u00e2n gi\u1ea3i l\u00e0 trung t\u00e2m c\u1ee7a c\u00f4ng ngh\u1ec7 HRMS. N\u00f3 mang l\u1ea1i \u0111\u1ed9 ch\u00ednh x\u00e1c h\u00e0ng \u0111\u1ea7u \u0111\u1ec3 ph\u00e1t hi\u1ec7n v\u00e0 \u0111\u1ebfm c\u00e1c nhi\u1ec7m v\u1ee5. C\u00e1c b\u1ed9 ph\u1eadn c\u1ee7a thi\u1ebft b\u1ecb, nh\u01b0 phong c\u00e1ch ph\u00e2n t\u00edch, thi\u1ebft l\u1eadp \u0111\u01b0\u1eddng d\u1eabn ion v\u00e0 b\u1ed9 b\u1eaft t\u00edn hi\u1ec7u, x\u00e2y d\u1ef1ng c\u01a1 s\u1edf cho vi\u1ec7c n\u00f3 gi\u1ea3i quy\u1ebft t\u1ed1t nh\u01b0 th\u1ebf n\u00e0o. C\u00e0i \u0111\u1eb7t trong khi ch\u1ea1y, ch\u1eb3ng h\u1ea1n nh\u01b0 t\u1ed1c \u0111\u1ed9 qu\u00e9t v\u00e0 \u0111i\u1ec1u ki\u1ec7n ngu\u1ed3n, c\u1ea7n ph\u00f9 h\u1ee3p \u0111\u1ec3 t\u1eadn d\u1ee5ng t\u1ed1i \u0111a. Cu\u1ed1i c\u00f9ng, \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao nh\u1ea5t \u0111\u1ebfn t\u1eeb vi\u1ec7c pha tr\u1ed9n ch\u0103m s\u00f3c ph\u1ea7n c\u1ee9ng v\u1edbi c\u00e1c b\u01b0\u1edbc ph\u1ea7n m\u1ec1m th\u00f4ng minh. H\u1ed7n h\u1ee3p n\u00e0y m\u1edf r\u1ed9ng nh\u1eefng g\u00ec quang ph\u1ed5 kh\u1ed1i l\u01b0\u1ee3ng c\u00f3 th\u1ec3 l\u00e0m trong c\u00e1c khu v\u1ef1c c\u1ea7n k\u1ebft qu\u1ea3 ch\u1eafc ch\u1eafn \u1edf m\u1ee9c th\u1ea5p.<\/p>\n Q1: Gi\u00e1 tr\u1ecb \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao trong ph\u1ed5 \u0111o kh\u1ed1i l\u01b0\u1ee3ng \u0111\u01b0\u1ee3c coi l\u00e0 g\u00ec? Q2: Ph\u1ed5 \u0111o kh\u1ed1i l\u01b0\u1ee3ng \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng cho ph\u00e2n t\u00edch \u0111\u1ecbnh l\u01b0\u1ee3ng th\u01b0\u1eddng xuy\u00ean kh\u00f4ng? Q3: M\u1ed9t d\u1ee5ng c\u1ee5 \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao n\u00ean \u0111\u01b0\u1ee3c hi\u1ec7u chu\u1ea9n bao l\u00e2u? In high resolution mass spectrometry (HRMS), resolution means the device’s skill in telling apart ions with almost the same mass-to-charge (m\/z) values. This skill matters a lot for spotting compounds correctly, particularly in tricky mixtures. In math terms, resolution shows as R = m\/\u0394m. Here, “m” stands for the m\/z at the main peak, […]<\/p>\n","protected":false},"author":1,"featured_media":4261,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[38,37],"tags":[],"class_list":["post-4265","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-industry-information","category-news"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts\/4265","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/comments?post=4265"}],"version-history":[{"count":4,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts\/4265\/revisions"}],"predecessor-version":[{"id":4273,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts\/4265\/revisions\/4273"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/media\/4261"}],"wp:attachment":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/media?parent=4265"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/categories?post=4265"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/tags?post=4265"}],"curies":[{"name":"WP","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}L\u00e0m th\u1ebf n\u00e0o c\u00e1c c\u00f4ng c\u1ee5 ph\u1ea7n m\u1ec1m c\u00f3 th\u1ec3 c\u1ea3i thi\u1ec7n \u0111\u1ed9 ph\u00e2n gi\u1ea3i sau khi mua l\u1ea1i?<\/strong><\/h3>\n
PERSEE: M\u1ed9t \u0111\u1ed1i t\u00e1c \u0111\u00e1ng tin c\u1eady trong c\u00e1c gi\u1ea3i ph\u00e1p thi\u1ebft b\u1ecb ph\u00e2n t\u00edch<\/strong><\/h2>\n
Nh\u1eadn x\u00e9t t\u00f3m t\u1eaft v\u1ec1 \u0111\u1ecbnh ngh\u0129a v\u00e0 \u00e1p d\u1ee5ng Ngh\u1ecb quy\u1ebft HRMS<\/strong><\/h2>\n
C\u00e2u h\u1ecfi th\u01b0\u1eddng g\u1eb7p<\/strong><\/h2>\n
\n<\/strong>A1: Th\u00f4ng th\u01b0\u1eddng, c\u00f4ng su\u1ea5t gi\u1ea3i quy\u1ebft l\u1edbn h\u01a1n 10.000 (m \/ \u0394m t\u1ea1i FWHM) \u0111\u01b0\u1ee3c coi l\u00e0 \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao; M\u1ed9t s\u1ed1 thi\u1ebft b\u1ecb v\u01b0\u1ee3t qu\u00e1 100.000 t\u00f9y thu\u1ed9c v\u00e0o lo\u1ea1i m\u00e1y ph\u00e2n t\u00edch.<\/p>\n
\n<\/strong>A2: V\u00e2ng, HRMS cung c\u1ea5p t\u00ednh ch\u1ecdn l\u1ecdc tuy\u1ec7t v\u1eddi n\u00e2ng cao \u0111\u1ed9 ch\u00ednh x\u00e1c \u0111\u1ecbnh l\u01b0\u1ee3ng ngay c\u1ea3 trong ma tr\u1eadn ph\u1ee9c t\u1ea1p; Tuy nhi\u00ean, vi\u1ec7c ph\u00e1t tri\u1ec3n ph\u01b0\u01a1ng ph\u00e1p ph\u1ea3i t\u00ednh \u0111\u1ebfn c\u00e1c th\u00f4ng s\u1ed1 c\u1ee5 th\u1ec3 c\u1ee7a d\u1ee5ng c\u1ee5.<\/p>\n
\n<\/strong>A3: T\u1ea7n s\u1ed1 hi\u1ec7u chu\u1ea9n ph\u1ee5 thu\u1ed9c v\u00e0o c\u01b0\u1eddng \u0111\u1ed9 s\u1eed d\u1ee5ng nh\u01b0ng n\u00f3i chung n\u00ean \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n h\u00e0ng ng\u00e0y ho\u1eb7c tr\u01b0\u1edbc m\u1ed7i l\u00f4 ph\u00e2n t\u00edch \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o vi\u1ec7c g\u00e1n kh\u1ed1i l\u01b0\u1ee3ng ch\u00ednh x\u00e1c v\u00e0 hi\u1ec7u su\u1ea5t nh\u1ea5t qu\u00e1n.<\/p>","protected":false},"excerpt":{"rendered":"