{"id":4249,"date":"2026-01-06T00:00:19","date_gmt":"2026-01-05T16:00:19","guid":{"rendered":"https:\/\/www.pgeneral.com\/?p=4249"},"modified":"2026-01-08T12:42:29","modified_gmt":"2026-01-08T04:42:29","slug":"how-size-exclusion-chromatography-enhances-analytical-accuracy","status":"publish","type":"post","link":"https:\/\/www.pgeneral.com\/vi\/news\/how-size-exclusion-chromatography-enhances-analytical-accuracy\/","title":{"rendered":"C\u00e1ch nhi\u1ec5m s\u1eafc lo\u1ea1i tr\u1eeb k\u00edch th\u01b0\u1edbc c\u1ea3i thi\u1ec7n \u0111\u1ed9 ch\u00ednh x\u00e1c ph\u00e2n t\u00edch"},"content":{"rendered":"

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\"C\u00e1ch<\/div>\n

Nh\u00e3n s\u1eafc h\u1ecdc lo\u1ea1i tr\u1eeb k\u00edch th\u01b0\u1edbc (SEC), c\u00f2n \u0111\u01b0\u1ee3c g\u1ecdi l\u00e0 nh\u00e3n s\u1eafc h\u1ecdc th\u00e2m nh\u1eadp gel (GPC), \u0111\u00f3ng vai tr\u00f2 l\u00e0 m\u1ed9t ph\u01b0\u01a1ng ph\u00e1p ph\u00e2n t\u00edch ch\u00ednh \u0111\u1ec3 ph\u00e2n lo\u1ea1i c\u00e1c ph\u00e2n t\u1eed l\u1edbn tinh khi\u1ebft theo k\u00edch th\u01b0\u1edbc th\u1ee7y l\u1ef1c c\u1ee7a ch\u00fang. \u00dd t\u01b0\u1edfng ch\u00ednh d\u1ef1a v\u00e0o s\u1ef1 kh\u00e1c bi\u1ec7t v\u1eadt l\u00fd, kh\u00f4ng ph\u1ea3i h\u00f3a h\u1ecdc: c\u00e1c h\u1ea1t \u0111\u01b0\u1ee3c t\u00e1ch ra th\u00f4ng qua s\u1ef1 lo\u1ea1i tr\u1eeb kh\u00e1c nhau t\u1eeb thi\u1ebft l\u1eadp x\u1ed1p c\u1ee7a giai \u0111o\u1ea1n t\u0129nh. C\u00e1c h\u1ea1t l\u1edbn h\u01a1n xu\u1ea5t hi\u1ec7n tr\u01b0\u1edbc v\u00ec ch\u00fang kh\u00f4ng th\u1ec3 v\u00e0o l\u1ed7 ch\u00e2n l\u00f4ng trong giai \u0111o\u1ea1n t\u0129nh. Trong khi \u0111\u00f3, c\u00e1c h\u1ea1t nh\u1ecf h\u01a1n tr\u01b0\u1ee3t v\u00e0o c\u00e1c l\u1ed7 ch\u00e2n l\u00f4ng \u0111\u00f3 v\u00e0 di chuy\u1ec3n ch\u1eadm h\u01a1n d\u1ecdc theo c\u1ed9t. \u0110i\u1ec1u n\u00e0y d\u1eabn \u0111\u1ebfn th\u1eddi gian d\u00e0i h\u01a1n tr\u01b0\u1edbc khi ch\u00fang tho\u00e1t kh\u1ecfi.<\/p>\n

C\u00e1ch ti\u1ebfp c\u1eadn n\u00e0y mang l\u1ea1i l\u1ee3i \u00edch \u0111\u1eb7c bi\u1ec7t cho SEC khi nghi\u00ean c\u1ee9u c\u00e1c polymer, protein, kh\u00e1ng th\u1ec3 v\u00e0 h\u1ea1t nano. N\u00f3 l\u00e0m nh\u01b0 v\u1eady m\u00e0 kh\u00f4ng thay \u0111\u1ed5i h\u00ecnh th\u1ee9c t\u1ef1 nhi\u00ean c\u1ee7a ch\u00fang ho\u1eb7c c\u1ea7n b\u1ea5t k\u1ef3 li\u00ean k\u1ebft h\u00f3a h\u1ecdc n\u00e0o. Nh\u01b0 Held v\u00e0 Kilz l\u01b0u \u00fd, \"Nh\u00e3n s\u1eafc h\u1ecdc lo\u1ea1i tr\u1eeb k\u00edch th\u01b0\u1edbc l\u00e0 k\u1ef9 thu\u1eadt \u0111\u1eb7c tr\u01b0ng quan tr\u1ecdng nh\u1ea5t cho c\u00e1c ph\u00e2n t\u1eed v\u0129 \u0111\u1ea1i.\"<\/p>\n

Nh\u1eefng th\u00f4ng s\u1ed1 n\u00e0o l\u00e0 quan tr\u1ecdng cho \u0111\u1ed9 ch\u00ednh x\u00e1c ph\u00e2n t\u00edch trong SEC?<\/strong><\/h2>\n

B\u1ea1n c\u1ea7n ch\u1ecdn giai \u0111o\u1ea1n di \u0111\u1ed9ng m\u1ed9t c\u00e1ch c\u1ea9n th\u1eadn \u0111\u1ec3 gi\u1eef m\u1eabu trong h\u00ecnh d\u1ea1ng t\u1ed1t. Trang \u0111i\u1ec3m c\u1ee7a b\u1ed9 \u0111\u1ec7m ph\u1ea3i h\u1ed7 tr\u1ee3 m\u1eabu’ s \u1ed5n \u0111\u1ecbnh v\u00e0 d\u1eebng b\u1ea5t k\u1ef3 r\u00e0ng bu\u1ed9c kh\u00f4ng mong mu\u1ed1n. V\u00ed d\u1ee5, protein ph\u1ea3n \u1ee9ng m\u1ea1nh m\u1ebd v\u1edbi s\u1ee9c m\u1ea1nh c\u1ee7a ion v\u00e0 m\u1ee9c pH. Nh\u1eefng y\u1ebfu t\u1ed1 n\u00e0y tr\u1ef1c ti\u1ebfp \u0111\u1ecbnh h\u00ecnh h\u00ecnh d\u1ea1ng c\u1ee7a ch\u00fang v\u00e0 c\u00e1ch ch\u00fang d\u00ednh d\u00e1n xung quanh trong c\u1ed9t. Th\u00eam m\u1ed9t s\u1ed1 ch\u1ea5t ph\u1ee5 gia h\u1eefu c\u01a1 c\u00f3 th\u1ec3 l\u00e0m gi\u1ea3m t\u00e1c d\u1ee5ng ch\u1ed1ng th\u1ee7y d\u00ednh. \u0110i\u1ec1u n\u00e0y \u0111\u1eb7c bi\u1ec7t h\u1eefu \u00edch khi l\u00e0m vi\u1ec7c v\u1edbi c\u00e1c polymer sinh h\u1ecdc.<\/p>\n

T\u1ea1i sao t\u1ef7 l\u1ec7 d\u00f2ng ch\u1ea3y ph\u1ea3i \u0111\u01b0\u1ee3c t\u1ed1i \u01b0u h\u00f3a cho \u0111\u1ed9 ph\u00e2n gi\u1ea3i t\u1ed1t nh\u1ea5t?<\/strong><\/h3>\n

T\u1ed1c \u0111\u1ed9 d\u00f2ng ch\u1ea3y \u0111\u00f3ng m\u1ed9t vai tr\u00f2 quan tr\u1ecdng trong c\u1ea3 \u0111\u1ed9 s\u1eafc n\u00e9t c\u1ee7a s\u1ef1 t\u00e1ch bi\u1ec7t v\u00e0 t\u1ed1c \u0111\u1ed9 c\u1ee7a qu\u00e1 tr\u00ecnh. C\u00e1c d\u00f2ng ch\u1ea3y nhanh h\u01a1n l\u00e0m gi\u1ea3m th\u1eddi gian c\u1ea7n thi\u1ebft cho ph\u00e2n t\u00edch, nh\u01b0ng ch\u00fang c\u00f3 th\u1ec3 l\u00e0m t\u1ed5n h\u1ea1i \u0111\u1ebfn \u0111\u1ed9 r\u00f5 r\u00e0ng c\u1ee7a s\u1ef1 t\u00e1ch. \u0110i\u1ec1u n\u00e0y x\u1ea3y ra ch\u1ee7 y\u1ebfu \u0111\u1ed1i v\u1edbi c\u00e1c h\u1ea1t g\u1ea7n gi\u1edbi h\u1ea1n lo\u1ea1i tr\u1eeb. M\u1eb7t kh\u00e1c, d\u00f2ng ch\u1ea3y ch\u1eadm h\u01a1n l\u00e0m t\u0103ng ch\u1ea5t l\u01b0\u1ee3ng t\u00e1ch nh\u01b0ng k\u00e9o d\u00e0i t\u1ed5ng th\u1eddi gian. V\u00ec v\u1eady, b\u1ea1n ph\u1ea3i t\u00ecm m\u1ed9t trung t\u00e2m t\u1ed1t. B\u1eb1ng c\u00e1ch n\u00e0y, b\u1ea1n c\u00f3 \u0111\u01b0\u1ee3c k\u1ebft qu\u1ea3 ph\u00e2n t\u00edch m\u1ea1nh m\u1ebd m\u00e0 kh\u00f4ng m\u1ea5t hi\u1ec7u qu\u1ea3.<\/p>\n

\u0110i\u1ec1u chu\u1ea9n l\u00e0 quan tr\u1ecdng nh\u01b0 th\u1ebf n\u00e0o trong \u01b0\u1edbc t\u00ednh tr\u1ecdng l\u01b0\u1ee3ng ph\u00e2n t\u1eed?<\/strong><\/h3>\n

B\u1ea3n th\u00e2n SEC kh\u00f4ng cung c\u1ea5p tr\u1ecdng l\u01b0\u1ee3ng ph\u00e2n t\u1eed ch\u00ednh x\u00e1c. Thay v\u00e0o \u0111\u00f3, n\u00f3 \u0111o\u00e1n ch\u00fang b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng \u0111\u01b0\u1eddng cong \u0111\u01b0\u1ee3c l\u00e0m t\u1eeb c\u00e1c m\u1eabu ti\u00eau chu\u1ea9n. B\u1eb1ng c\u00e1ch hi\u1ec7u chu\u1ea9n v\u1edbi c\u00e1c ti\u00eau chu\u1ea9n \u0111\u01b0\u1ee3c bi\u1ebft \u0111\u1ebfn, b\u1ea1n c\u00f3 th\u1ec3 li\u00ean k\u1ebft kh\u1ed1i l\u01b0\u1ee3ng elution v\u1edbi tr\u1ecdng l\u01b0\u1ee3ng ph\u00e2n t\u1eed. \u0110i\u1ec1u n\u00e0y \u0111\u00fang \u0111\u1eb7c bi\u1ec7t khi \u00e1p d\u1ee5ng c\u00e1c ph\u01b0\u01a1ng ph\u00e1p hi\u1ec7u chu\u1ea9n r\u1ed9ng r\u00e3i, ch\u1eb3ng h\u1ea1n nh\u01b0 ph\u01b0\u01a1ng tr\u00ecnh Grubisic.<\/p>\n

L\u00e0m th\u1ebf n\u00e0o SEC c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c \u00e1p d\u1ee5ng \u0111\u1ec3 n\u00e2ng cao \u0111\u1ed9 ch\u00ednh x\u00e1c ph\u00e2n t\u00edch tr\u00ean c\u00e1c lo\u1ea1i m\u1eabu kh\u00e1c nhau?<\/strong><\/h2>\n

Trong s\u1ef1 ph\u00e1t tri\u1ec3n c\u1ee7a d\u01b0\u1ee3c ph\u1ea9m sinh h\u1ecdc, ph\u00e1t hi\u1ec7n c\u00e1c c\u1ee5m protein n\u1ed5i b\u1eadt nh\u01b0 m\u1ed9t ki\u1ec3m tra ch\u1ea5t l\u01b0\u1ee3ng quan tr\u1ecdng. SEC ch\u1ecdn c\u00e1c t\u1ed5ng h\u1ee3p tr\u1ecdng l\u01b0\u1ee3ng ph\u00e2n t\u1eed cao quan tr\u1ecdng r\u1ea5t nhi\u1ec1u trong vi\u1ec7c ki\u1ec3m so\u00e1t ch\u1ea5t l\u01b0\u1ee3ng d\u01b0\u1ee3c ph\u1ea9m sinh h\u1ecdc. N\u00f3 ph\u00e1 v\u1ee1 c\u00e1c \u0111\u01a1n ch\u1ea5t t\u1eeb c\u00e1c c\u1eb7p ho\u1eb7c c\u00e1c nh\u00f3m l\u1edbn h\u01a1n, gi\u00fap \u0111\u00e1nh gi\u00e1 protein \u1ed5n \u0111\u1ecbnh nh\u01b0 th\u1ebf n\u00e0o d\u01b0\u1edbi c\u00e1c thi\u1ebft l\u1eadp kh\u00e1c nhau. Th\u00eam v\u00e0o \u0111\u00f3, th\u00f4ng qua \u0111o l\u01b0\u1eddng c\u1ea9n th\u1eadn, n\u00f3 ph\u00e2n bi\u1ec7t c\u00e1c h\u00ecnh th\u1ee9c \u0111\u01a1n l\u1ebb ch\u00ednh t\u1eeb c\u00e1c bit b\u1ecb ph\u00e1 v\u1ee1.<\/p>\n

L\u1ee3i \u00edch g\u00ec m\u00e0 SEC cung c\u1ea5p cho c\u00e1c h\u1ea1t nano v\u00e0 k\u1ebft h\u1ee3p sinh h\u1ecdc?<\/strong><\/h3>\n

C\u00e1c h\u1ea1t nano v\u00e0 k\u1ebft h\u1ee3p sinh h\u1ecdc mang l\u1ea1i c\u00e1c v\u1ea5n \u0111\u1ec1 kh\u00f3 kh\u0103n v\u00ec s\u1ef1 trang \u0111i\u1ec3m \u0111a d\u1ea1ng c\u1ee7a ch\u00fang. SEC t\u1ecfa s\u00e1ng trong khu v\u1ef1c n\u00e0y v\u00ec n\u00f3 s\u1eafp x\u1ebfp ch\u00fang theo b\u00e1n k\u00ednh th\u1ee7y l\u1ef1c h\u1ecdc. \u0110\u1eb7c bi\u1ec7t, n\u00f3 chia c\u00e1c h\u1ea1t nano theo b\u00e1n k\u00ednh th\u1ee7y l\u1ef1c h\u1ecdc c\u1ee7a ch\u00fang, gi\u00fap ki\u1ec3m tra t\u00ednh \u0111\u1ed3ng \u0111\u1ec1u k\u00edch th\u01b0\u1edbc. \u0110\u1ed1i v\u1edbi nh\u1eefng th\u1ee9 nh\u01b0 k\u1ebft h\u1ee3p kh\u00e1ng th\u1ec3-thu\u1ed1c ho\u1eb7c protein li\u00ean k\u1ebft v\u1edbi PEG, n\u00f3 c\u00f3 th\u1ec3 t\u00e1ch bi\u1ec7t c\u00e1c phi\u00ean b\u1ea3n li\u00ean k\u1ebft t\u1eeb nh\u1eefng phi\u00ean b\u1ea3n kh\u00f4ng li\u00ean k\u1ebft.<\/p>\n

Y\u1ebfu t\u1ed1 c\u00f4ng c\u1ee5 n\u00e0o n\u00ean \u0111\u01b0\u1ee3c xem x\u00e9t trong thi\u1ebft l\u1eadp SEC ch\u00ednh x\u00e1c cao?<\/strong><\/h2>\n

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\"K\u00edch<\/div>\n

L\u1ef1a ch\u1ecdn c\u1ed9t ph\u1ee5 thu\u1ed9c v\u00e0o ph\u1ea1m vi tr\u1ecdng l\u01b0\u1ee3ng ph\u00e2n t\u1eed d\u1ef1 ki\u1ebfn v\u00e0 n\u00f3 ph\u00f9 h\u1ee3p h\u00f3a h\u1ecdc nh\u01b0 th\u1ebf n\u00e0o. C\u00e1c c\u1ed9t ph\u1ea3i ph\u00f9 h\u1ee3p v\u1edbi ph\u1ea1m vi tr\u1ecdng l\u01b0\u1ee3ng ph\u00e2n t\u1eed d\u1ef1 ki\u1ebfn \u200b\u200b\u0111\u1ec3 \u0111\u1ea1t \u0111\u01b0\u1ee3c \u0111\u1ed9 r\u00f5 r\u00e0ng t\u1ed1t nh\u1ea5t c\u00f3 th\u1ec3. Ngo\u00e0i ra, c\u00e1c thi\u1ebft l\u1eadp d\u00e0nh cho c\u00e1c h\u1ec7 th\u1ed1ng d\u1ef1a tr\u00ean n\u01b0\u1edbc ho\u1ea1t \u0111\u1ed9ng t\u1ed1t nh\u1ea5t cho protein v\u00e0 polysaccharides. Ng\u01b0\u1ee3c l\u1ea1i, c\u00e1c c\u1ed9t x\u1eed l\u00fd dung m\u00f4i h\u1eefu c\u01a1 ph\u00f9 h\u1ee3p v\u1edbi c\u00e1c polymer nh\u00e2n t\u1ea1o nh\u01b0 PMMA ho\u1eb7c polystyrene. Chi\u1ec1u d\u00e0i v\u00e0 chi\u1ec1u r\u1ed9ng c\u1ee7a c\u1ed9t c\u0169ng \u0111\u00f3ng vai tr\u00f2 v\u00e0o \u0111\u1ed9 s\u1eafc n\u00e9t c\u1ee7a s\u1ef1 t\u00e1ch v\u00e0 bao nhi\u00eau m\u1eabu n\u00f3 c\u00f3 th\u1ec3 x\u1eed l\u00fd c\u00f9ng m\u1ed9t l\u00fac.<\/p>\n

M\u00e1y d\u00f2 n\u00e0o th\u00eam gi\u00e1 tr\u1ecb cho gi\u1ea3i th\u00edch c\u1ee7a SEC?<\/strong><\/h3>\n

SEC th\u01b0\u1eddng h\u1ee3p t\u00e1c v\u1edbi c\u00e1c m\u00e1y d\u00f2 kh\u00e1c nhau \u0111\u1ec3 l\u00e0m cho k\u1ebft qu\u1ea3 d\u1ec5 hi\u1ec3u h\u01a1n:<\/p>\n

Ch\u1ec9 s\u1ed1 kh\u00fac x\u1ea1 (RI): \u0110i\u1ec1u n\u00e0y cung c\u1ea5p ph\u00e1t hi\u1ec7n r\u1ed9ng r\u00e3i cho c\u00e1c polymer, carbohydrate v\u00e0 protein thi\u1ebfu c\u00e1c b\u1ed9 ph\u1eadn h\u1ea5p th\u1ee5 m\u00e0u s\u1eafc.<\/p>\n

UV \/ Vis: N\u00f3 cho ph\u00e9p ph\u00e1t hi\u1ec7n m\u1ee5c ti\u00eau b\u1eb1ng c\u00e1ch c\u00e1c m\u1eabu h\u1ea5p th\u1ee5 \u00e1nh s\u00e1ng.<\/p>\n

MALS: \u0110i\u1ec1u n\u00e0y cung c\u1ea5p tr\u1ecdng l\u01b0\u1ee3ng ph\u00e2n t\u1eed th\u1ef1c s\u1ef1 m\u00e0 kh\u00f4ng c\u1ea7n c\u00e1c m\u1eabu ti\u00eau chu\u1ea9n \u0111\u1ec3 hi\u1ec7u chu\u1ea9n.<\/p>\n

Ch\u1ea1y ch\u00fang c\u00f9ng nhau cho ph\u00e9p b\u1ea1n thu th\u1eadp d\u1eef li\u1ec7u c\u00f9ng m\u1ed9t l\u00fac. K\u1ebft qu\u1ea3 l\u00e0, n\u00f3 li\u00ean k\u1ebft m\u1ee9c \u0111\u1ed9 n\u1ed3ng \u0111\u1ed9 v\u1edbi c\u00e1c \u0111\u1eb7c \u0111i\u1ec3m c\u1ea5u tr\u00fac, cho ph\u00e9p ph\u00e1 v\u1ee1 \u0111\u1ea7y \u0111\u1ee7 t\u1eeb ch\u1ec9 m\u1ed9t ch\u1ea1y th\u1eed nghi\u1ec7m. C\u00e1ch ti\u1ebfp c\u1eadn k\u1ebft h\u1ee3p n\u00e0y t\u0103ng c\u01b0\u1eddng \u0111\u00e1ng k\u1ec3 \u0111\u1ed9 tin c\u1eady c\u1ee7a d\u1eef li\u1ec7u b\u1ea1n nh\u1eadn \u0111\u01b0\u1ee3c.<\/p>\n

Ai c\u00f3 th\u1ec3 cung c\u1ea5p c\u00e1c gi\u1ea3i ph\u00e1p thi\u1ebft b\u1ecb SEC \u0111\u00e1ng tin c\u1eady v\u00e0 s\u00e1ng t\u1ea1o?<\/strong><\/h2>\n

N\u1ebfu b\u1ea1n mu\u1ed1n \u0111\u01b0a SEC v\u00e0o ph\u00f2ng th\u00ed nghi\u1ec7m c\u1ee7a b\u1ea1n v\u1edbi \u0111\u1ed9 ch\u00ednh x\u00e1c v\u00e0 linh ho\u1ea1t h\u00e0ng \u0111\u1ea7u, PERSEE<\/strong><\/a> cung c\u1ea5p m\u1ed9t l\u1ef1a ch\u1ecdn xu\u1ea5t s\u1eafc c\u1ee7a c\u00e1c c\u00f4ng c\u1ee5 nhi\u1ec5m s\u1eafc \u0111\u01b0\u1ee3c thi\u1ebft k\u1ebf cho c\u1ea3 ph\u00f2ng th\u00ed nghi\u1ec7m nghi\u00ean c\u1ee9u v\u00e0 c\u00e1c thi\u1ebft l\u1eadp c\u00f4ng nghi\u1ec7p. \u0110\u01b0\u1ee3c th\u00e0nh l\u1eadp nh\u01b0 m\u1ed9t c\u00f4ng ty c\u00f4ng ngh\u1ec7 cao t\u1eadp trung v\u00e0o c\u00e1c c\u00f4ng c\u1ee5 khoa h\u1ecdc v\u00e0o n\u0103m 1991, PERSEE \u0111\u00e3 gi\u1eef ch\u00e2n th\u00e0nh v\u1edbi vi\u1ec7c th\u00fac \u0111\u1ea9y ph\u00eda tr\u01b0\u1edbc trong c\u00e1c l\u0129nh v\u1ef1c nh\u01b0 HPLC, GC, FTIR<\/strong><\/a>UV \/ Vis quang ph\u1ed5, v\u00e0 quang ph\u1ed5 nguy\u00ean t\u1eed<\/strong><\/a>S\u1ef1 t\u1eadp trung m\u1ea1nh m\u1ebd c\u1ee7a h\u1ecd v\u00e0o nghi\u00ean c\u1ee9u cho th\u1ea5y r\u00f5 r\u00e0ng h\u01a1n 30% nh\u00e2n vi\u00ean l\u00e0m vi\u1ec7c trong R & amp; D, lu\u00f4n c\u1ea3i thi\u1ec7n s\u1ea3n ph\u1ea9m \u0111\u1ec3 \u0111\u00e1p \u1ee9ng nh\u1eefng g\u00ec ng\u01b0\u1eddi d\u00f9ng c\u1ea7n khi m\u1ecdi th\u1ee9 thay \u0111\u1ed5i theo th\u1eddi gian.<\/p>\n

Trong lineup c\u1ee7a h\u1ecd, c\u00e1c H\u1ec7 th\u1ed1ng nhi\u1ec5m s\u1eafc ch\u1ea5t l\u1ecfng hi\u1ec7u su\u1ea5t cao L600<\/u><\/strong><\/a> th\u1ef1c s\u1ef1 n\u1ed5i b\u1eadt. C\u1ea5u tr\u00fac v\u1eefng ch\u1eafc c\u1ee7a n\u00f3 l\u00e0m cho n\u00f3 ho\u00e0n h\u1ea3o cho c\u00e1c nhi\u1ec7m v\u1ee5 SEC chi ti\u1ebft. H\u1ec7 th\u1ed1ng \u0111\u1ea3m b\u1ea3o ki\u1ec3m so\u00e1t ch\u00ednh x\u00e1c d\u00f2ng ch\u1ea3y, \u0111i\u1ec1u quan tr\u1ecdng \u0111\u1ec3 c\u00f3 \u0111\u01b0\u1ee3c k\u1ebft qu\u1ea3 l\u1eb7p l\u1ea1i trong s\u1ef1 t\u00e1ch d\u1ef1a tr\u00ean k\u00edch th\u01b0\u1edbc ph\u00e2n t\u1eed. Ngo\u00e0i ra, PERSEE h\u1ed7 tr\u1ee3 c\u00e1c nh\u00e0 khoa h\u1ecdc tr\u00ean to\u00e0n th\u1ebf gi\u1edbi v\u1edbi s\u1ef1 gi\u00fap \u0111\u1ee1 th\u1ef1c t\u1ebf trong vi\u1ec7c thi\u1ebft l\u1eadp, \u0111\u00e0o t\u1ea1o v\u00e0 b\u1ea3o tr\u00ec. H\u1ecd l\u00e0m \u0111i\u1ec1u n\u00e0y th\u00f4ng qua m\u1ed9t m\u1ea1ng l\u01b0\u1edbi v\u1eefng ch\u1eafc c\u1ee7a c\u00e1c nh\u00e0 ph\u00e2n ph\u1ed1i tr\u00ean to\u00e0n c\u1ea7u, l\u00e0m cho h\u1ed7 tr\u1ee3 d\u1ec5 d\u00e0ng truy c\u1eadp b\u1ea5t k\u1ec3 b\u1ea1n \u0111ang \u1edf \u0111\u00e2u.<\/p>\n

SEC \u0111\u00f3ng g\u00f3p nh\u01b0 th\u1ebf n\u00e0o cho \u0111\u1ed9 ch\u00ednh x\u00e1c ph\u00e2n t\u00edch tr\u00ean kh\u1eafp c\u00e1c ng\u00e0nh c\u00f4ng nghi\u1ec7p?<\/strong><\/h2>\n

SEC’ K\u1ef9 n\u0103ng k\u00e9o ra c\u00e1c ph\u00e2n t\u1eed ch\u1ec9 b\u1eb1ng k\u00edch th\u01b0\u1edbc c\u1ee7a ch\u00fang, t\u1ea5t c\u1ea3 trong khi duy tr\u00ec c\u00e1c \u0111i\u1ec1u ki\u1ec7n b\u1ea3n \u0111\u1ecba, bi\u1ebfn n\u00f3 th\u00e0nh m\u1ed9t c\u00f4ng c\u1ee5 c\u1ea7n thi\u1ebft \u0111\u1ec3 ki\u1ec3m tra \u0111\u1ed9 tinh khi\u1ebft. N\u00f3 ph\u00e1t hi\u1ec7n m\u1ed9t c\u00e1ch \u0111\u00e1ng tin c\u1eady s\u1ef1 kh\u00e1c bi\u1ec7t gi\u1eefa c\u00e1c lo\u1ea1i ph\u00e2n t\u1eed kh\u00e1c nhau, l\u00e0m t\u0103ng s\u1ee9c m\u1ea1nh c\u1ee7a ki\u1ec3m tra \u0111\u1ed9 tinh khi\u1ebft theo nhi\u1ec1u c\u00e1ch. SEC cho th\u1ea5y s\u1ef1 h\u1eefu \u00edch r\u1ed9ng r\u00e3i c\u1ee7a n\u00f3 t\u1eeb \u0111\u1eb7c tr\u01b0ng c\u00e1c polymer trong khoa h\u1ecdc v\u1eadt li\u1ec7u, \u0111\u1ec3 t\u00ecm c\u00e1c ch\u1ea5t t\u1ed5ng h\u1ee3p trong d\u01b0\u1ee3c ph\u1ea9m sinh h\u1ecdc v\u00e0 \u0111\u1ecbnh k\u00edch th\u01b0\u1edbc c\u00e1c h\u1ea1t nano trong y h\u1ecdc nano. N\u00f3 ph\u00f9 h\u1ee3p tr\u01a1n tru v\u1edbi nhi\u1ec1u l\u0129nh v\u1ef1c, bao g\u1ed3m d\u01b0\u1ee3c ph\u1ea9m, khoa h\u1ecdc v\u1eadt li\u1ec7u v\u00e0 c\u00f4ng ngh\u1ec7 sinh h\u1ecdc. Trong c\u00e1c l\u0129nh v\u1ef1c n\u00e0y, n\u00f3 x\u1eed l\u00fd c\u00e1c nhu c\u1ea7u \u0111a d\u1ea1ng v\u1edbi hi\u1ec7u su\u1ea5t nh\u1ea5t qu\u00e1n, th\u00edch \u1ee9ng v\u1edbi c\u00e1c th\u00e1ch th\u1ee9c c\u1ee5 th\u1ec3 trong m\u1ed7i l\u0129nh v\u1ef1c trong khi duy tr\u00ec ti\u00eau chu\u1ea9n cao v\u1ec1 \u0111\u1ed9 ch\u00ednh x\u00e1c v\u00e0 \u0111\u1ed9 tin c\u1eady. Li\u00ean k\u1ebft SEC v\u1edbi c\u00e1c c\u00f4ng c\u1ee5 nh\u01b0 MALS ho\u1eb7c UV c\u00f3 kh\u1ea3 n\u0103ng \u0111o l\u01b0\u1ee3ng l\u00ean m\u1ed9t m\u1ee9c cao h\u01a1n. Vi\u1ec7c k\u1ebft n\u1ed1i n\u00e0y t\u0103ng c\u01b0\u1eddng \u0111\u1ed9 tin c\u1eady c\u1ee7a c\u00e1c con s\u1ed1 b\u1ea1n nh\u1eadn \u0111\u01b0\u1ee3c.<\/p>\n

C\u00e2u h\u1ecfi th\u01b0\u1eddng g\u1eb7p<\/strong><\/h2>\n

Q1: L\u00e0m th\u1ebf n\u00e0o m\u00e0 chromatography lo\u1ea1i tr\u1eeb k\u00edch th\u01b0\u1edbc kh\u00e1c v\u1edbi chromatography kh\u00e1c<\/strong> k\u1ef9 thu\u1eadt?
\nA1: Kh\u00f4ng gi\u1ed1ng nh\u01b0 trao \u0111\u1ed5i ion ho\u1eb7c nhi\u1ec5m s\u1eafc h\u1ecdc pha ng\u01b0\u1ee3c d\u1ef1a v\u00e0o c\u00e1c t\u01b0\u01a1ng t\u00e1c h\u00f3a h\u1ecdc, SEC t\u00e1ch c\u00e1c ch\u1ea5t ph\u00e2n t\u00edch ch\u1ec9 d\u1ef1a tr\u00ean k\u00edch th\u01b0\u1edbc th\u1ee7y l\u1ef1c h\u1ecdc c\u1ee7a ch\u00fang b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng ma tr\u1eadn x\u1ed1p.<\/p>\n

Q2: Nh\u1eefng th\u00e1ch th\u1ee9c ph\u1ed5 bi\u1ebfn trong vi\u1ec7c \u0111\u1ea1t \u0111\u01b0\u1ee3c k\u1ebft qu\u1ea3 ch\u00ednh x\u00e1c b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng SEC l\u00e0 g\u00ec?
\n<\/strong>A2: Th\u00e1ch th\u1ee9c bao g\u1ed3m c\u00e1c t\u01b0\u01a1ng t\u00e1c kh\u00f4ng c\u1ee5 th\u1ec3 v\u1edbi giai \u0111o\u1ea1n t\u0129nh, ti\u00eau chu\u1ea9n hi\u1ec7u chu\u1ea9n kh\u00f4ng ph\u00f9 h\u1ee3p cho c\u00e1c m\u1eabu ph\u1ee9c t\u1ea1p v\u00e0 t\u1ef7 l\u1ec7 d\u00f2ng ch\u1ea3y kh\u00f4ng t\u1ed1i \u01b0u ho\u1eb7c \u0111i\u1ec1u ki\u1ec7n giai \u0111o\u1ea1n di \u0111\u1ed9ng.<\/p>\n

Q3: C\u00f3 th\u1ec3 s\u1eed d\u1ee5ng nhi\u1ec5m s\u1eafc lo\u1ea1i tr\u1eeb k\u00edch th\u01b0\u1edbc cho c\u1ea3 n\u01b0\u1edbc v\u00e0 h\u1eefu c\u01a1<\/strong> h\u1ec7 th\u1ed1ng?
\nA3: C\u00f3, t\u00f9y thu\u1ed9c v\u00e0o v\u1eadt li\u1ec7u giai \u0111o\u1ea1n t\u0129nh \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng; C\u00e1c c\u1ed9t t\u01b0\u01a1ng th\u00edch n\u01b0\u1edbc ph\u00f9 h\u1ee3p v\u1edbi protein v\u00e0 polysaccharides trong khi c\u00e1c c\u1ed9t t\u01b0\u01a1ng th\u00edch h\u1eefu c\u01a1 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng cho c\u00e1c polymer t\u1ed5ng h\u1ee3p nh\u01b0 polystyrene ho\u1eb7c PMMA.<\/p>","protected":false},"excerpt":{"rendered":"

  Size exclusion chromatography (SEC), also known as gel permeation chromatography (GPC), serves as a key analytical method for sorting macromolecules purely by their hydrodynamic size. The main idea relies on physical differences, not chemical ones: particles get separated through varying exclusion from the porous setup of the stationary phase. Bigger particles come out first […]<\/p>\n","protected":false},"author":1,"featured_media":4244,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[38,37],"tags":[],"class_list":["post-4249","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-industry-information","category-news"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts\/4249","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/comments?post=4249"}],"version-history":[{"count":1,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts\/4249\/revisions"}],"predecessor-version":[{"id":4250,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts\/4249\/revisions\/4250"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/media\/4244"}],"wp:attachment":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/media?parent=4249"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/categories?post=4249"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/tags?post=4249"}],"curies":[{"name":"WP","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}