{"id":4226,"date":"2025-12-25T00:00:06","date_gmt":"2025-12-24T16:00:06","guid":{"rendered":"https:\/\/www.pgeneral.com\/?p=4226"},"modified":"2025-12-25T11:08:15","modified_gmt":"2025-12-25T03:08:15","slug":"mastering-the-spectrophotometer-stepwise-operation-of-uv-vis-systems","status":"publish","type":"post","link":"https:\/\/www.pgeneral.com\/vi\/news\/mastering-the-spectrophotometer-stepwise-operation-of-uv-vis-systems\/","title":{"rendered":"N\u1eafm v\u1eefng ho\u1ea1t \u0111\u1ed9ng theo b\u01b0\u1edbc c\u1ee7a m\u00e1y quang ph\u1ed5 c\u1ee7a h\u1ec7 th\u1ed1ng UV-VIS"},"content":{"rendered":"

 <\/p>\n

\"N\u1eafm<\/div>\n

Hi\u1ec3u ho\u1ea1t \u0111\u1ed9ng c\u1ee7a m\u1ed9t m\u00e1y quang ph\u1ed5 UV-VIS b\u1eaft \u0111\u1ea7u v\u1edbi vi\u1ec7c bi\u1ebft c\u00e1c \u00fd t\u01b0\u1edfng c\u01a1 b\u1ea3n c\u1ee7a n\u00f3. Ph\u01b0\u01a1ng ph\u00e1p n\u00e0y \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng r\u1ed9ng r\u00e3i trong ph\u00f2ng th\u00ed nghi\u1ec7m h\u00f3a h\u1ecdc v\u00e0 sinh h\u1ecdc. N\u00f3 ho\u1ea1t \u0111\u1ed9ng t\u1ed1t v\u00ec n\u00f3 cung c\u1ea5p c\u00e1c con s\u1ed1 \u0111\u00e1ng tin c\u1eady v\u00e0 ch\u00ednh x\u00e1c. M\u1ed7i ch\u1ea5t h\u00f3a h\u1ecdc l\u1ea5y v\u00e0o, \u0111\u1ec3 qua, ho\u1eb7c nh\u1ea3y tr\u1edf l\u1ea1i \u00e1nh s\u00e1ng, \u0111\u00f3 l\u00e0 m\u1ed9t h\u00ecnh th\u1ee9c c\u1ee7a s\u00f3ng \u0111i\u1ec7n t\u1eeb, tr\u00ean c\u00e1c ph\u1ea1m vi b\u01b0\u1edbc s\u00f3ng nh\u1ea5t \u0111\u1ecbnh. M\u00e1y quang ph\u1ed5 UV-VIS<\/strong><\/a> s\u1eed d\u1ee5ng th\u1ef1c t\u1ebf n\u00e0y \u0111\u1ec3 t\u00ecm ra l\u01b0\u1ee3ng ho\u1eb7c lo\u1ea1i v\u1eadt li\u1ec7u trong h\u1ed7n h\u1ee3p ch\u1ea5t l\u1ecfng.<\/p>\n

C\u01a1 s\u1edf l\u00fd thuy\u1ebft c\u1ee7a s\u1ef1 h\u1ea5p th\u1ee5 UV-VIS<\/strong><\/h3>\n

S\u1ef1 h\u1ea5p th\u1ee5 c\u1ee7a tia c\u1ef1c t\u00edm v\u00e0 \u00e1nh s\u00e1ng c\u00f3 th\u1ec3 nh\u00ecn th\u1ea5y b\u1edfi c\u00e1c ph\u00e2n t\u1eed \u0111\u1ebfn t\u1eeb s\u1ef1 thay \u0111\u1ed5i c\u1ee7a c\u00e1c electron. \u0110\u1eb7c bi\u1ec7t, c\u00e1c electron nh\u1ea3y t\u1eeb c\u00e1c \u0111i\u1ec3m n\u0103ng l\u01b0\u1ee3ng th\u1ea5p trong c\u00e1c ph\u00e2n t\u1eed sang c\u00e1c \u0111i\u1ec3m n\u0103ng l\u01b0\u1ee3ng cao h\u01a1n. C\u00e1c nh\u00f3m \u0111\u01b0\u1ee3c g\u1ecdi l\u00e0 chromophores, nh\u01b0 li\u00ean k\u1ebft k\u00e9p gi\u1eefa carbon ho\u1eb7c c\u1ea5u tr\u00fac v\u00f2ng trong c\u00e1c ch\u1ea5t th\u01a1m, g\u00e2y ra s\u1ef1 h\u1ea5p th\u1ee5 n\u00e0y. H\u1ecd c\u0169ng \u0111\u1ecbnh h\u00ecnh c\u00e1c \u0111\u1eb7c \u0111i\u1ec3m ch\u00ednh c\u1ee7a m\u1ed9t ph\u00e2n t\u1eed’ S m\u1eabu \u00e1nh s\u00e1ng.<\/p>\n

C\u00e1c tr\u00e1i phi\u1ebfu li\u00ean k\u1ebft l\u00e0m t\u0103ng b\u01b0\u1edbc s\u00f3ng ch\u00ednh c\u1ee7a s\u1ef1 h\u1ea5p th\u1ee5, \u0111\u01b0\u1ee3c g\u1ecdi l\u00e0 \u03bbmax. \u0110i\u1ec1u n\u00e0y x\u1ea3y ra b\u1edfi v\u00ec c\u00e1c tr\u1ea1ng th\u00e1i ph\u1ea5n kh\u00edch tr\u1edf n\u00ean \u1ed5n \u0111\u1ecbnh h\u01a1n. C\u00e1c nh\u00f3m b\u00ean kh\u00e1c nhau c\u00f3 \u00e1nh s\u00e1ng \u1edf c\u00e1c khu v\u1ef1c \u0111i\u1ec3n h\u00ecnh. \u0110i\u1ec1u n\u00e0y gi\u00fap ki\u1ec3m tra c\u01a1 b\u1ea3n v\u1ec1 nh\u1eefng g\u00ec c\u00f3 m\u1eb7t. B\u00ean d\u1ef1a tr\u00ean s\u1ed1 theo \u0111\u1ecbnh lu\u1eadt Beer-Lambert: A = \u03b5 \u00d7 c \u00d7 l. \u1ede \u0111\u00e2y: A vi\u1ebft t\u1eaft cho \u0111\u1ed9 h\u1ea5p th\u1ee5, \u03b5 c\u00f3 ngh\u0129a l\u00e0 \u0111\u1ed9 h\u1ea5p th\u1ee5 molar, c l\u00e0 n\u1ed3ng \u0111\u1ed9 (mol \/ L), l l\u00e0 chi\u1ec1u d\u00e0i \u0111\u01b0\u1eddng d\u1eabn (cm). Li\u00ean k\u1ebft n\u00e0y \u1edf th\u1eb3ng trong m\u1ed9t khu v\u1ef1c h\u1ea5p th\u1ee5 \u0111\u01b0\u1ee3c thi\u1ebft l\u1eadp, th\u01b0\u1eddng l\u00e0 0,2-1,0 AU. \u0110\u01b0\u1eddng th\u1eb3ng \u0111\u00f3 l\u00e0 ch\u00eca kh\u00f3a cho s\u1ed1 \u0111\u1ebfm ch\u00ednh x\u00e1c.<\/p>\n

C\u00e1c th\u00e0nh ph\u1ea7n d\u1ee5ng c\u1ee5 c\u1ee7a m\u00e1y quang ph\u1ed5 UV-VIS<\/strong><\/h3>\n

 <\/p>\n

\"TU600<\/div>\n

\u0110\u1ec3 c\u00f3 \u0111\u01b0\u1ee3c \u0111\u1ecdc ch\u00ednh x\u00e1c v\u00e0 \u1ed5n \u0111\u1ecbnh, m\u00e1y’ s build bao g\u1ed3m m\u1ed9t s\u1ed1 ph\u1ea7n ch\u00ednh:<\/p>\n

Ngu\u1ed3n \u00e1nh s\u00e1ng<\/strong>\u0110\u00e8n deuterium x\u1eed l\u00fd tia c\u1ef1c t\u00edm (190-400 nm), \u0111\u00e8n vonfram-halogen c\u00f3 th\u1ec3 nh\u00ecn th\u1ea5y (400-700 nm)<\/p>\n

M\u00e1y monochromators<\/strong>H\u1ecd s\u1eed d\u1ee5ng k\u00ednh k\u00ednh ho\u1eb7c l\u01b0\u1edbi x\u00fac x\u1ea1 \u0111\u1ec3 ch\u1ecdn b\u01b0\u1edbc s\u00f3ng nh\u1ea5t \u0111\u1ecbnh, b\u01b0\u1edbc s\u00f3ng \u0111\u01b0\u1ee3c ch\u1ecdn \u0111i qua khe tho\u00e1t \u0111\u1ebfn m\u1eabu.<\/p>\n

\u00c1nh s\u00e1ng t\u1eeb ngu\u1ed3n \u0111i qua m\u1ed9t khe l\u1ed1i v\u00e0o trong monochromator. C\u00e1ch n\u1ee9t n\u00e0y l\u00e0m cho ch\u00f9m c\u00f3 k\u00edch th\u01b0\u1edbc kh\u1ea3 thi. Ti\u1ebfp theo, n\u00f3 \u0111i qua m\u1ed9t l\u01b0\u1edbi x\u00fac x\u1ea1. \u1ede \u0111\u00f3, \u00e1nh s\u00e1ng \u0111\u01b0\u1ee3c chia th\u00e0nh c\u00e1c d\u1ea3i h\u1eb9p c\u1ee7a \u00e1nh s\u00e1ng \u0111\u01a1n m\u00e0u.<\/p>\n

Khung m\u1eabu<\/strong>H\u1ecd gi\u1eef cuvettes, th\u01b0\u1eddng \u0111\u01b0\u1ee3c l\u00e0m t\u1eeb th\u1ea1ch anh cho tia c\u1ef1c t\u00edm, th\u1ee7y tinh cho VIS, ho\u1eb7c nh\u1ef1a, chi\u1ec1u d\u00e0i \u0111\u01b0\u1eddng \u0111i, th\u01b0\u1eddng l\u00e0 1 cm, c\u1ea7n ph\u1ea3i gi\u1eef nh\u01b0 nhau cho so s\u00e1nh c\u00f4ng b\u1eb1ng.<\/p>\n

M\u00e1y d\u00f2<\/strong>Photodiode v\u00e0 \u1ed1ng photomultiplier bi\u1ebfn \u00e1nh s\u00e1ng qua th\u00e0nh t\u00edn hi\u1ec7u \u0111i\u1ec7n, ph\u1ea7n m\u1ec1m x\u1eed l\u00fd t\u00edn hi\u1ec7u thay \u0111\u1ed5i \u0111i\u1ec1u n\u00e0y th\u00e0nh s\u1ed1 h\u1ea5p th\u1ee5 ho\u1eb7c m\u00f4 h\u00ecnh \u00e1nh s\u00e1ng \u0111\u1ea7y \u0111\u1ee7.<\/p>\n

Y\u00eau c\u1ea7u tr\u01b0\u1edbc khi ho\u1ea1t \u0111\u1ed9ng v\u00e0 thi\u1ebft l\u1eadp h\u1ec7 th\u1ed1ng<\/strong><\/h2>\n

Tr\u01b0\u1edbc b\u1ea5t k\u1ef3 th\u1eed nghi\u1ec7m n\u00e0o, thi\u1ebft l\u1eadp t\u1ed1t v\u00e0 ki\u1ec3m tra \u0111\u1ea3m b\u1ea3o r\u1eb1ng quang ph\u1ed5 cung c\u1ea5p k\u1ebft qu\u1ea3 v\u1eefng ch\u1eafc. \u0110i\u1ec1u n\u00e0y bao g\u1ed3m c\u00e1c thi\u1ebft l\u1eadp c\u01a1 h\u1ecdc, d\u1ef1a tr\u00ean \u00e1nh s\u00e1ng v\u00e0 ch\u01b0\u01a1ng tr\u00ecnh.<\/p>\n

Ti\u00eau chu\u1ea9n d\u1ee5ng c\u1ee5 v\u00e0 s\u1eeda ch\u1eefa d\u00f2ng c\u01a1 b\u1ea3n<\/strong><\/h3>\n

Ki\u1ec3m tra r\u1ea5t quan tr\u1ecdng cho vi\u1ec7c \u0111\u1ecdc th\u1ef1c s\u1ef1:<\/p>\n

\u0110\u1ed9 ch\u00ednh x\u00e1c b\u01b0\u1edbc s\u00f3ng<\/strong>Ki\u1ec3m tra v\u1edbi c\u00e1c m\u1ee5c ki\u1ec3m tra \u0111\u01b0\u1ee3c ph\u00ea duy\u1ec7t nh\u01b0 b\u1ed9 l\u1ecdc oxyt holmium, gi\u1ed1ng nh\u01b0 c\u00e1c c\u00f4ng c\u1ee5 kh\u00e1c, ch\u00fang c\u1ea7n \u0111\u00e1nh gi\u00e1 v\u00e0 b\u1eb1ng ch\u1ee9ng \u1ed5n \u0111\u1ecbnh. \u0110\u1ed1i v\u1edbi m\u00e1y quang ph\u1ed5, c\u00e1c th\u1eed nghi\u1ec7m bao g\u1ed3m \u0111\u1ed9 ch\u00ednh x\u00e1c quang h\u1ecdc (\u0111\u1ed9 th\u1eb3ng h\u1ea5p th\u1ee5), \u0111\u1ed9 ch\u00ednh x\u00e1c b\u01b0\u1edbc s\u00f3ng, b\u0103ng th\u00f4ng v\u00e0 \u00e1nh s\u00e1ng l\u1ea1c.<\/p>\n

S\u1eeda ch\u1eefa c\u01a1 s\u1edf<\/strong>N\u00f3 kh\u1eafc ph\u1ee5c c\u00e1c v\u1ea5n \u0111\u1ec1 t\u1eeb \u00e1nh s\u00e1ng l\u1ea1c l\u00f9ng v\u00e0 \u00e2m thanh n\u1ec1n. B\u1ea1n l\u00e0m \u0111i\u1ec1u n\u00e0y b\u1eb1ng c\u00e1ch qu\u00e9t m\u1ed9t v\u00f9ng tr\u1ed1ng, \u0111\u00f3 l\u00e0 m\u1ed9t cuvette ch\u1ec9 v\u1edbi dung m\u00f4i, tr\u00ean b\u01b0\u1edbc s\u00f3ng.<\/p>\n

L\u1ef1a ch\u1ecdn v\u00e0 chu\u1ea9n b\u1ecb c\u00e1c c\u1ed1c v\u00e0 m\u1eabu<\/strong><\/h3>\n

V\u1eadt li\u1ec7u ph\u00f9 h\u1ee3p r\u1ea5t quan tr\u1ecdng:<\/p>\n\n\n\n\n<\/colgroup>\n\n\n\n\n\n
Khu v\u1ef1c quang ph\u1ed5<\/th>\nLo\u1ea1i Cuvette<\/th>\nGhi ch\u00fa<\/th>\n<\/tr>\n<\/thead>\n
UV (< 300 nm)<\/td>\nTh\u1ea1ch anh<\/td>\nS\u1ef1 minh b\u1ea1ch cao<\/td>\n<\/tr>\n
VIS (> 320 nm)<\/td>\nTh\u1ee7y tinh \/ nh\u1ef1a<\/td>\nNh\u1ef1a kh\u00f4ng ph\u00f9 h\u1ee3p v\u1edbi UV<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

H\u00e3y \u0111\u1ea3m b\u1ea3o c\u00e1c m\u1eabu r\u00f5 r\u00e0ng v\u00e0 \u0111\u1ed3ng \u0111\u1ec1u.<\/p>\n

Gi\u1eef n\u1ed3ng \u0111\u1ed9 \u1edf khu v\u1ef1c h\u1ea5p th\u1ee5 th\u1eb3ng (0,2-1,0 AU).<\/p>\n

C\u1ea5u h\u00ecnh ph\u1ea7n m\u1ec1m v\u00e0 thi\u1ebft l\u1eadp ph\u01b0\u01a1ng ph\u00e1p<\/strong><\/h3>\n

C\u00e1c m\u00e1y quang ph\u1ed5 m\u1edbi c\u00f3 c\u00e1c c\u00e1ch d\u1ef1a tr\u00ean ch\u01b0\u01a1ng tr\u00ecnh \u0111\u1ec3 x\u00e2y d\u1ef1ng c\u00e1c ph\u01b0\u01a1ng ph\u00e1p: thi\u1ebft l\u1eadp chi ti\u1ebft qu\u00e9t bao g\u1ed3m b\u01b0\u1edbc s\u00f3ng, t\u1ed1c \u0111\u1ed9 qu\u00e9t, chi\u1ec1u r\u1ed9ng khe, m\u00f4 h\u00ecnh l\u01b0u tr\u1eef \u0111\u1ec3 gi\u1eef cho c\u00e1c ph\u01b0\u01a1ng ph\u00e1p gi\u1ed1ng nhau trong c\u00e1c th\u1eed nghi\u1ec7m.<\/p>\n

V\u1eadn h\u00e0nh theo b\u01b0\u1edbc c\u1ee7a m\u00e1y quang ph\u1ed5 UV-VIS<\/strong><\/h2>\n

Sau khi thi\u1ebft l\u1eadp, m\u1ed9t d\u00f2ng ch\u1ea3y r\u00f5 r\u00e0ng t\u1eebng b\u01b0\u1edbc \u0111\u1ea3m b\u1ea3o d\u1eef li\u1ec7u \u0111\u1ebfn \u0111\u00fang. N\u00f3 \u0111i t\u1eeb vi\u1ec7c th\u00eam m\u1eabu cho k\u1ebft qu\u1ea3 \u0111\u1ecdc.<\/p>\n

Quy tr\u00ecnh b\u1eadt \u0111i\u1ec7n v\u00e0 s\u01b0\u1edfi \u1ea5m<\/strong><\/h3>\n

B\u1eaft \u0111\u1ea7u v\u1edbi kh\u1edfi \u0111\u1ed9ng m\u00e1y ch\u00ednh x\u00e1c: B\u1eadt c\u00f4ng c\u1ee5 v\u00e0 \u0111\u1ec3 \u0111\u00e8n \u1ea5m l\u00ean, kho\u1ea3ng 20-30 ph\u00fat. \u0110i\u1ec1u n\u00e0y cho s\u1ef1 \u1ed5n \u0111\u1ecbnh nhi\u1ec7t. Ki\u1ec3m tra t\u00edch h\u1ee3p x\u00e1c nh\u1eadn thi\u1ebft l\u1eadp, c\u01b0\u1eddng \u0111\u1ed9 \u0111\u00e8n v\u00e0 c\u00f4ng vi\u1ec7c d\u00f2.<\/p>\n

\u0110o l\u01b0\u1eddng tr\u1ed1ng v\u00e0 l\u00e0m 0 d\u1ee5ng c\u1ee5<\/strong><\/h3>\n

M\u1ed9t ch\u1ea5t l\u1ecfng tr\u1ed1ng, ch\u1ec9 c\u00f3 dung m\u00f4i, \u0111\u01b0\u1ee3c \u0111o tr\u01b0\u1edbc. N\u00f3 thi\u1ebft l\u1eadp \u0111i\u1ec3m b\u1eaft \u0111\u1ea7u h\u1ea5p th\u1ee5 \u1edf m\u1ed7i b\u01b0\u1edbc s\u00f3ng. Lu\u00f4n \u0111\u1eb7t cuvettes theo c\u00f9ng m\u1ed9t c\u00e1ch. \u0110i\u1ec1u n\u00e0y tr\u00e1nh c\u00e1c v\u1ea5n \u0111\u1ec1 u\u1ed1n cong \u00e1nh s\u00e1ng.<\/p>\n

Giao th\u1ee9c \u0111o m\u1eabu<\/strong><\/h3>\n

C\u00e1ch b\u1ea1n \u0111o l\u01b0\u1eddng ph\u1ee5 thu\u1ed9c v\u00e0o nh\u1eefng g\u00ec b\u1ea1n mu\u1ed1n t\u00ecm th\u1ea5y. C\u00e1c ch\u1ebf \u0111\u1ed9 kh\u00e1c nhau ph\u00f9 h\u1ee3p v\u1edbi nhu c\u1ea7u kh\u00e1c nhau.<\/p>\n

Ch\u1ebf \u0111\u1ed9 ph\u00e2n t\u00edch b\u01b0\u1edbc s\u00f3ng \u0111\u01a1n<\/strong><\/h4>\n

Ch\u1ebf \u0111\u1ed9 n\u00e0y ph\u00f9 h\u1ee3p v\u1edbi ki\u1ec3m tra d\u1ef1a tr\u00ean s\u1ed1 ch\u1ee7 y\u1ebfu: thi\u1ebft l\u1eadp c\u00f4ng c\u1ee5 \u0111\u1ec3 ph\u00e2n t\u00edch’ s \u03bbmax v\u00e0 \u0111\u1ecdc m\u1eabu’ S\u1ef1 h\u1ea5p th\u1ee5. Sau \u0111\u00f3, k\u1ebft h\u1ee3p n\u00f3 v\u1edbi m\u1ed9t \u0111\u01b0\u1eddng hi\u1ec7u chu\u1ea9n.<\/p>\n

Ch\u1ebf \u0111\u1ed9 qu\u00e9t ph\u1ed5 \u0111\u1ea7y \u0111\u1ee7<\/strong><\/h4>\n

\u0110i\u1ec1u n\u00e0y ho\u1ea1t \u0111\u1ed9ng t\u1ed1t nh\u1ea5t cho c\u00e1c nghi\u00ean c\u1ee9u c\u01a1 b\u1ea3n: ch\u1ee5p to\u00e0n b\u1ed9 m\u00f4 h\u00ecnh h\u1ea5p th\u1ee5, n\u00f3i r\u1eb1ng 200-800 nm, \u0111\u1ec9nh \u0111i\u1ec3m \u0111\u1ec3 x\u00e1c minh ch\u1ea5t ph\u00e2n t\u00edch l\u00e0 g\u00ec ho\u1eb7c t\u00ecm c\u00e1c b\u1ed9 ph\u1eadn kh\u00f4ng mong mu\u1ed1n.<\/p>\n

Ho\u1ea1t \u0111\u1ed9ng ch\u1ebf \u0111\u1ed9 Kinetics (n\u1ebfu \u00e1p d\u1ee5ng)<\/strong><\/h4>\n

\u0110\u1ec3 theo d\u00f5i ph\u1ea3n \u1ee9ng: kh\u00f3a b\u01b0\u1edbc s\u00f3ng \u1edf \u03bbmax, \u0111\u1ecdc \u0111\u1ed9 h\u1ea5p th\u1ee5 qua kho\u1ea3ng th\u1eddi gian. \u0110i\u1ec1u n\u00e0y cho th\u1ea5y t\u1ed1c \u0111\u1ed9 ph\u1ea3n \u1ee9ng.<\/p>\n

K\u1ef9 thu\u1eadt x\u1eed l\u00fd v\u00e0 gi\u1ea3i th\u00edch d\u1eef li\u1ec7u<\/strong><\/h2>\n

Sau khi \u0111\u1ecdc, ph\u00e2n t\u00edch r\u00fat ra c\u00e1c s\u1ef1 ki\u1ec7n h\u1eefu \u00edch t\u1eeb th\u00f4ng tin h\u1ea5p th\u1ee5.<\/p>\n

X\u00e2y d\u1ef1ng \u0111\u01b0\u1eddng cong hi\u1ec7u chu\u1ea9n cho ph\u00e2n t\u00edch \u0111\u1ecbnh l\u01b0\u1ee3ng<\/strong><\/h3>\n

C\u00e1c b\u01b0\u1edbc l\u00e0 t\u1ea1o ra ch\u1ea5t l\u1ecfng ti\u00eau chu\u1ea9n v\u1edbi s\u1ed1 l\u01b0\u1ee3ng \u0111\u00e3 bi\u1ebft, l\u1ea5y \u0111\u1ecdc \u0111\u1ed9 h\u1ea5p th\u1ee5 c\u1ee7a ch\u00fang, v\u1ebd bi\u1ec3u \u0111\u1ed3 \u0111\u1ed9 h\u1ea5p th\u1ee5 ch\u1ed1ng l\u1ea1i n\u1ed3ng \u0111\u1ed9 v\u00e0 s\u1eed d\u1ee5ng to\u00e1n h\u1ecdc \u0111\u01b0\u1eddng th\u1eb3ng \u0111\u1ec3 c\u00f3 \u0111\u01b0\u1ee3c c\u00f4ng th\u1ee9c hi\u1ec7u chu\u1ea9n.<\/p>\n

Ph\u00e2n t\u00edch quang ph\u1ed5 cho nh\u1eefng hi\u1ec3u bi\u1ebft ch\u1ea5t l\u01b0\u1ee3ng<\/strong><\/h3>\n

Nh\u00ecn v\u00e0o c\u00e1c h\u00ecnh th\u1ee9c, k\u00edch th\u01b0\u1edbc v\u00e0 \u0111i\u1ec3m \u0111\u1ec9nh: r\u00f5 r\u00e0ng, th\u1eadm ch\u00ed \u0111\u1ec9nh th\u01b0\u1eddng c\u00f3 ngh\u0129a l\u00e0 m\u1eabu tinh khi\u1ebft, th\u1eed c\u00e1c ph\u01b0\u01a1ng ph\u00e1p ph\u00e1i sinh \u0111\u1ec3 s\u1eafp x\u1ebfp c\u00e1c \u0111\u1ec9nh ch\u1ed3ng ch\u00e9o trong c\u00e1c thi\u1ebft l\u1eadp h\u1ed7n h\u1ee3p.<\/p>\n

Xu\u1ea5t kh\u1ea9u, ti\u1ebft ki\u1ec7m v\u00e0 b\u00e1o c\u00e1o k\u1ebft qu\u1ea3<\/strong><\/h3>\n

M\u00e1y’ cho ph\u00e9p b\u1ea1n g\u1eedi d\u1eef li\u1ec7u d\u01b0\u1edbi nhi\u1ec1u d\u1ea1ng CSV cho c\u00e1c ch\u01b0\u01a1ng tr\u00ecnh b\u1ea3ng, PDF cho b\u00e1o c\u00e1o, c\u00e1c bi\u1ec3u m\u1eabu \u0111\u1eb7c bi\u1ec7t cho ki\u1ec3m tra sau v\u00e0 li\u00ean k\u1ebft LIMS \u0111\u1ec3 theo d\u00f5i v\u00e0 \u0111\u00e1nh gi\u00e1.<\/p>\n

Giao th\u1ee9c b\u1ea3o tr\u00ec v\u00e0 H\u01b0\u1edbng d\u1eabn kh\u1eafc ph\u1ee5c s\u1ef1 c\u1ed1<\/strong><\/h2>\n

\u0110\u1ec3 l\u00e0m vi\u1ec7c t\u1ed1t l\u00e2u d\u00e0i, ch\u0103m s\u00f3c th\u01b0\u1eddng xuy\u00ean v\u00e0 s\u1eeda ch\u1eefa nhanh ch\u00f3ng gi\u00fap m\u1ecdi th\u1ee9 ch\u1ea1y t\u1ed1t.<\/p>\n

Th\u1ef1c h\u00e0nh b\u1ea3o tr\u00ec th\u01b0\u1eddng xuy\u00ean<\/strong><\/h3>\n

C\u00e1c c\u00f4ng vi\u1ec7c bao g\u1ed3m trao \u0111\u1ed5i \u0111\u00e8n deuterium \/ vonfram d\u1ef1a tr\u00ean th\u1eddi gian s\u1eed d\u1ee5ng, lau cuvettes, \u0111i\u1ec3m m\u1eabu v\u00e0 \u0111\u01b0\u1eddng d\u1eabn \u00e1nh s\u00e1ng th\u01b0\u1eddng xuy\u00ean, ki\u1ec3m tra, ki\u1ec3m so\u00e1t ch\u1ea5t l\u01b0\u1ee3ng, ch\u1ee9ng minh ph\u01b0\u01a1ng ph\u00e1p v\u00e0 thi\u1ebft l\u1eadp l\u00e0 c\u1ea7n thi\u1ebft. \u0110i\u1ec1u n\u00e0y \u0111\u1ebfn t\u1eeb c\u00e1c quy t\u1eafc ph\u00f2ng th\u00ed nghi\u1ec7m t\u1ed1t ho\u1eb7c c\u00e1c quy t\u1eafc \u0111\u01b0\u1ee3c thi\u1ebft l\u1eadp b\u1edfi lu\u1eadt ph\u00e1p.<\/p>\n

Gi\u1ea3i quy\u1ebft c\u00e1c v\u1ea5n \u0111\u1ec1 hi\u1ec7u su\u1ea5t ph\u1ed5 bi\u1ebfn<\/strong><\/h3>\n

L\u00fd do c\u00f3 th\u1ec3 l\u00e0 s\u1ef1 b\u1ea5t \u0111\u1ed3ng c\u1ee7a \u0111\u00e8n, c\u00e1c b\u1ed9 ph\u1eadn \u00e1nh s\u00e1ng b\u1ea9n v\u00e0 gi\u00e1n \u0111o\u1ea1n \u0111i\u1ec7n.<\/p>\n

\u0110\u1ecdc b\u01b0\u1edbc s\u00f3ng kh\u00f4ng ch\u00ednh x\u00e1c<\/strong><\/h4>\n

\u0110i\u1ec1u n\u00e0y th\u01b0\u1eddng xu\u1ea5t ph\u00e1t t\u1eeb monochromator ra kh\u1ecfi d\u00f2ng v\u00e0 ti\u00eau chu\u1ea9n ki\u1ec3m tra m\u00f2n<\/p>\n

Nh\u1ea5n m\u1ea1nh PERSEE<\/a> L\u00e0 m\u1ed9t nh\u00e0 s\u1ea3n xu\u1ea5t \u0111\u00e1ng tin c\u1eady trong thi\u1ebft b\u1ecb ph\u00e2n t\u00edch<\/strong><\/h2>\n

PERSEE \u0111\u00e3 x\u00e2y d\u1ef1ng m\u1ed9t t\u00ean m\u1ea1nh m\u1ebd nh\u01b0 m\u1ed9t nh\u00e0 s\u1ea3n xu\u1ea5t h\u00e0ng \u0111\u1ea7u tr\u00ean to\u00e0n th\u1ebf gi\u1edbi c\u1ee7a c\u00e1c c\u00f4ng c\u1ee5 quang ph\u1ed5 ch\u00ednh x\u00e1c. C\u00f4ng ty<\/strong><\/a> t\u1eadp trung v\u00e0o c\u00e1c \u00fd t\u01b0\u1edfng m\u1edbi cho th\u1ea5y c\u00e1ch ch\u00fang pha tr\u1ed9n c\u00f4ng ngh\u1ec7 nh\u1eb9 v\u1edbi c\u00e1c ch\u01b0\u01a1ng tr\u00ecnh ph\u00e2n t\u00edch th\u00f4ng minh.<\/p>\n

T\u1ed5ng quan v\u1ec1 chuy\u00ean m\u00f4n c\u1ee7a PERSEE trong c\u00e1c gi\u1ea3i ph\u00e1p quang ph\u1ed5<\/strong><\/h3>\n

V\u1edbi nhi\u1ec1u n\u0103m l\u00e0m c\u00f4ng c\u1ee5 ph\u00e2n t\u00edch, PERSEE cung c\u1ea5p m\u00e1y quang ph\u1ed5 c\u1ee9ng, d\u1ec5 s\u1eed d\u1ee5ng. Ch\u00fang ph\u00f9 h\u1ee3p v\u1edbi c\u00e1c nhi\u1ec7m v\u1ee5 h\u00e0ng ng\u00e0y v\u00e0 nhu c\u1ea7u nghi\u00ean c\u1ee9u s\u00e2u s\u1eafc.<\/p>\n

M\u00f4 h\u00ecnh n\u1ed5i b\u1eadt: PERSEE M7 v\u00e0 G5 Series<\/strong><\/h3>\n

\u0110i\u1ec1u n\u00e0y ph\u00f9 h\u1ee3p v\u1edbi c\u00e1c nhi\u1ec7m v\u1ee5 chi ti\u1ebft cao Thi\u1ebft l\u1eadp hai \u0111\u00e8n cung c\u1ea5p ph\u1ea1m vi \u00e1nh s\u00e1ng r\u1ed9ng r\u00e3i, phong c\u00e1ch hai ch\u00f9m c\u1eaft gi\u1ea3m s\u1ef1 thay \u0111\u1ed5i c\u01a1 b\u1ea3n v\u00e0 s\u1eeda ch\u1eefa nh\u1eefng thay \u0111\u1ed5i tr\u1ef1c ti\u1ebfp.<\/p>\n

D\u00f2ng G5GC<\/a> H\u1ec7 th\u1ed1ng UV-VIS<\/strong><\/h4>\n

\u0110\u01b0\u1ee3c thi\u1ebft k\u1ebf cho m\u00f4i tr\u01b0\u1eddng l\u01b0u l\u01b0\u1ee3ng truy c\u1eadp cao, h\u1ec7 th\u1ed1ng cung c\u1ea5p x\u1eed l\u00fd t\u1ef1 \u0111\u1ed9ng cho c\u00e1c m\u1eabu kh\u1ed1i l\u01b0\u1ee3ng l\u1edbn th\u00f4ng qua m\u1ed9t ch\u01b0\u01a1ng tr\u00ecnh m\u1ea1nh m\u1ebd v\u00e0 th\u00edch \u1ee9ng c\u00f3 t\u00ednh n\u0103ng ph\u01b0\u01a1ng ph\u00e1p c\u00f3 th\u1ec3 c\u1ea5u h\u00ecnh r\u1ed9ng r\u00e3i.<\/p>\n

Nh\u1eefng c\u00e2n nh\u1eafc ho\u1ea1t \u0111\u1ed9ng ch\u00ednh<\/strong><\/h2>\n

K\u1ebft qu\u1ea3 th\u1ef1c s\u1ef1 v\u00e0 l\u1eb7p \u0111i l\u1eb7p l\u1ea1i ph\u1ee5 thu\u1ed9c v\u00e0o c\u00e1c b\u01b0\u1edbc c\u1ea9n th\u1eadn: L\u00e0m c\u00e1c s\u1eeda ch\u1eefa c\u01a1 b\u1ea3n \u0111\u00fang, Ch\u1ecdn c\u00e1c cuvettes ph\u00f9 h\u1ee3p cho khu v\u1ef1c \u00e1nh s\u00e1ng, ki\u1ec3m tra ph\u01b0\u01a1ng ph\u00e1p b\u00e2y gi\u1edd v\u00e0 sau \u0111\u00f3 v\u1edbi c\u00e1c ti\u00eau chu\u1ea9n \u0111\u01b0\u1ee3c ph\u00ea duy\u1ec7t, Gi\u1eef d\u1eef li\u1ec7u t\u1ed1t v\u1ea5n \u0111\u1ec1 nh\u01b0 nhi\u1ec1u: l\u1eadp k\u1ebf ho\u1ea1ch thay \u0111\u1ed5i \u0111\u00e8n, l\u00e0m s\u1ea1ch c\u00e1c b\u1ed9 ph\u1eadn \u00e1nh s\u00e1ng th\u01b0\u1eddng xuy\u00ean, ch\u1ea1y th\u1eed nghi\u1ec7m h\u1ec7 th\u1ed1ng tr\u01b0\u1edbc khi<\/p>\n

C\u00e2u h\u1ecfi th\u01b0\u1eddng g\u1eb7p<\/strong><\/h2>\n

Q1: Ph\u1ea1m vi h\u1ea5p th\u1ee5 l\u00fd t\u01b0\u1edfng cho k\u1ebft qu\u1ea3 \u0111\u1ecbnh l\u01b0\u1ee3ng ch\u00ednh x\u00e1c l\u00e0 g\u00ec?
\n<\/strong>A1: Khu v\u1ef1c h\u1ea5p th\u1ee5 t\u1ed1t nh\u1ea5t l\u00e0 gi\u1eefa 0,2-1,0 AU. C\u00e1c gi\u00e1 tr\u1ecb v\u01b0\u1ee3t qu\u00e1 \u0111i\u1ec1u n\u00e0y c\u00f3 th\u1ec3 mang l\u1ea1i u\u1ed1n cong ho\u1eb7c t\u00edn hi\u1ec7u \u0111\u1ea7y \u0111\u1ee7.<\/p>\n

Q2: T\u00f4i c\u00f3 th\u1ec3 s\u1eed d\u1ee5ng c\u00e1c \u1ed1ng nh\u1ef1a \u0111\u1ec3 \u0111o trong khu v\u1ef1c UV kh\u00f4ng?
\n<\/strong>A2: C\u00e1c cuvette nh\u1ef1a th\u01b0\u1eddng kh\u00f4ng ho\u1ea1t \u0111\u1ed9ng d\u01b0\u1edbi 300 nm. H\u1ecd thi\u1ebfu \u0111\u1ed9 r\u00f5 r\u00e0ng UV t\u1ed1t. S\u1eed d\u1ee5ng cuvettes th\u1ea1ch anh cho c\u00f4ng vi\u1ec7c UV.<\/p>\n

Q3: T\u00f4i n\u00ean hi\u1ec7u chu\u1ea9n l\u1ea1i quang ph\u1ed5 c\u1ee7a m\u00ecnh bao l\u00e2u?
\n<\/strong>A3: Ki\u1ec3m tra l\u1ea1i h\u00e0ng th\u00e1ng trong khi s\u1eed d\u1ee5ng th\u01b0\u1eddng xuy\u00ean. H\u00e3y l\u00e0m \u0111i\u1ec1u \u0111\u00f3 nhi\u1ec1u h\u01a1n n\u1ebfu c\u00e1c b\u00e0i ki\u1ec3m tra ch\u00ednh \u0111ang \u0111\u1ebfn.<\/p>\n

 <\/p>","protected":false},"excerpt":{"rendered":"

  Understanding the operation of a UV-VIS spectrophotometer starts with knowing its basic ideas. This method finds wide use in chemistry and biology labs. It works well because it gives reliable and exact numbers. Every chemical substance takes in, lets through, or bounces back light, which is a form of electromagnetic waves, across certain wavelength […]<\/p>\n","protected":false},"author":1,"featured_media":4222,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[38,37],"tags":[],"class_list":["post-4226","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-industry-information","category-news"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts\/4226","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/comments?post=4226"}],"version-history":[{"count":1,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts\/4226\/revisions"}],"predecessor-version":[{"id":4227,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts\/4226\/revisions\/4227"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/media\/4222"}],"wp:attachment":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/media?parent=4226"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/categories?post=4226"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/tags?post=4226"}],"curies":[{"name":"WP","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}