{"id":3693,"date":"2025-07-03T11:50:49","date_gmt":"2025-07-03T03:50:49","guid":{"rendered":"https:\/\/www.pgeneral.com\/?p=3693"},"modified":"2025-07-03T16:42:50","modified_gmt":"2025-07-03T08:42:50","slug":"mastering-the-use-of-a-uv-vis-spectrometer-for-concentration-analysis","status":"publish","type":"post","link":"https:\/\/www.pgeneral.com\/vi\/news\/mastering-the-use-of-a-uv-vis-spectrometer-for-concentration-analysis\/","title":{"rendered":"N\u1eafm v\u1eefng vi\u1ec7c s\u1eed d\u1ee5ng UV Vis Spectrometer \u0111\u1ec3 ph\u00e2n t\u00edch n\u1ed3ng \u0111\u1ed9"},"content":{"rendered":"
<\/p>\n
Ph\u1ed5 \u0111o si\u00eau t\u00edm (UV Vis) l\u00e0 m\u1ed9t c\u00f4ng c\u1ee5 quan tr\u1ecdng. N\u00f3 gi\u00fap \u0111o l\u01b0\u1ee3ng v\u1eadt li\u1ec7u trong m\u1ed9t m\u1eabu b\u1eb1ng c\u00e1ch ki\u1ec3m tra \u00e1nh s\u00e1ng \u0111\u01b0\u1ee3c ng\u00e2m nh\u01b0 th\u1ebf n\u00e0o. M\u1ecdi ng\u01b0\u1eddi s\u1eed d\u1ee5ng n\u00f3 trong nhi\u1ec1u l\u0129nh v\u1ef1c, nh\u01b0 th\u1eed nghi\u1ec7m n\u01b0\u1edbc ho\u1eb7c ch\u1ebf t\u1ea1o thu\u1ed1c. N\u00f3 ch\u00ednh x\u00e1c, \u0111\u00e1ng tin c\u1eady v\u00e0 linh ho\u1ea1t. H\u01b0\u1edbng d\u1eabn n\u00e0y gi\u1ea3i th\u00edch c\u00e1ch s\u1eed d\u1ee5ng m\u00e1y \u0111o ph\u1ed5 UV Vis \u0111\u1ec3 \u0111o l\u01b0\u1ee3ng, t\u1eadp trung v\u00e0o c\u00e1c m\u00f4 h\u00ecnh T6U v\u00e0 T7 tuy\u1ec7t v\u1eddi c\u1ee7a Persee. B\u1eb1ng c\u00e1ch h\u1ecdc nh\u1eefng \u0111i\u1ec1u c\u01a1 b\u1ea3n, ph\u01b0\u01a1ng ph\u00e1p v\u00e0 c\u00e1ch s\u1eed d\u1ee5ng, b\u1ea1n c\u00f3 th\u1ec3 l\u00e0m cho c\u00e1c b\u00e0i ki\u1ec3m tra c\u1ee7a m\u00ecnh t\u1ed1t h\u01a1n v\u00e0 c\u00f3 \u0111\u01b0\u1ee3c k\u1ebft qu\u1ea3 th\u1ef1c s\u1ef1.<\/p>\n
UV Vis spectrometry s\u1eed d\u1ee5ng \u00e1nh s\u00e1ng \u0111\u1ec3 t\u00ecm hi\u1ec3u v\u1ec1 trang \u0111i\u1ec3m c\u1ee7a m\u1eabu. N\u00f3 ki\u1ec3m tra \u00e1nh s\u00e1ng ho\u1ea1t \u0111\u1ed9ng nh\u01b0 th\u1ebf n\u00e0o khi n\u00f3 ch\u1ea1m v\u00e0o v\u1eadt ch\u1ea5t. Ph\u1ea7n n\u00e0y bao g\u1ed3m c\u00e1c quy t\u1eafc ch\u00ednh, c\u00e1c m\u1ea3nh v\u00e0 nh\u1eefng \u0111i\u1ec1u b\u1ea1n \u0111o l\u01b0\u1eddng \u0111\u1ec3 hi\u1ec3u ph\u01b0\u01a1ng ph\u00e1p n\u00e0y.<\/p>\n
UV Vis quang ph\u1ed5 ki\u1ec3m tra bao nhi\u00eau \u00e1nh s\u00e1ng m\u1ed9t m\u1eabu h\u1ea5p th\u1ee5 ho\u1eb7c \u0111\u1ec3 qua. N\u00f3 ho\u1ea1t \u0111\u1ed9ng trong ph\u1ea1m vi \u00e1nh s\u00e1ng si\u00eau t\u00edm (190-400 nm) v\u00e0 c\u00f3 th\u1ec3 nh\u00ecn th\u1ea5y (400-800 nm). Khi \u00e1nh s\u00e1ng \u0111i qua m\u1ed9t m\u1eabu, c\u00e1c bit trong m\u1eabu l\u1ea5y s\u00f3ng \u00e1nh s\u00e1ng nh\u1ea5t \u0111\u1ecbnh. \u0110i\u1ec1u n\u00e0y l\u00e0m cho c\u00e1c electron nh\u1ea3y \u0111\u1ebfn c\u00e1c \u0111i\u1ec3m n\u0103ng l\u01b0\u1ee3ng cao h\u01a1n. Ch\u00fang t\u00f4i \u0111o l\u01b0\u1eddng s\u1ef1 h\u1ea5p th\u1ee5 n\u00e0y \u0111\u1ec3 t\u00ecm ra bao nhi\u00eau bit nh\u1ea5t \u0111\u1ecbnh - \u0111\u01b0\u1ee3c g\u1ecdi l\u00e0 chromophores - trong m\u1eabu. Ph\u01b0\u01a1ng ph\u00e1p n\u00e0y nhanh ch\u00f3ng v\u00e0 d\u1ec5 d\u00e0ng, ho\u00e0n h\u1ea3o cho c\u00e1c b\u00e0i ki\u1ec3m tra h\u00e0ng ng\u00e0y.<\/p>\n
M\u00e1y quang ph\u1ed5 UV Vis c\u00f3 m\u1ed9t v\u00e0i b\u1ed9 ph\u1eadn quan tr\u1ecdng l\u00e0m vi\u1ec7c c\u00f9ng nhau \u0111\u1ec3 cung c\u1ea5p th\u00f4ng tin ch\u00ednh x\u00e1c:<\/p>\n
\u0110\u1ed9 truy\u1ec1n (T) cho th\u1ea5y c\u00f3 bao nhi\u00eau \u00e1nh s\u00e1ng \u0111i qua m\u1ed9t m\u1eabu so v\u1edbi \u00e1nh s\u00e1ng b\u1eaft \u0111\u1ea7u. N\u00f3 \u0111\u01b0\u1ee3c hi\u1ec3n th\u1ecb nh\u01b0 m\u1ed9t ph\u1ea7n tr\u0103m (%T). H\u1ea5p th\u1ee5 (A) l\u00e0 ng\u01b0\u1ee3c l\u1ea1i, lo\u1ea1i. N\u00f3 \u0111\u01b0\u1ee3c t\u00ecm ra b\u1eb1ng A = -log(T). H\u1ea5p th\u1ee5 li\u00ean k\u1ebft tr\u1ef1c ti\u1ebfp v\u1edbi bao nhi\u00eau v\u1eadt li\u1ec7u c\u00f3 trong m\u1eabu. V\u00ec v\u1eady, \u0111\u00f3 l\u00e0 \u0111i\u1ec1u ch\u00ednh \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 \u0111o l\u01b0\u1eddng s\u1ed1 l\u01b0\u1ee3ng. Bi\u1ebft c\u00e1c thu\u1eadt ng\u1eef n\u00e0y gi\u00fap b\u1ea1n \u0111\u1ecdc \u0111\u00fang k\u1ebft qu\u1ea3 quang ph\u1ed5.<\/p>\n
B\u00e2y gi\u1edd b\u1ea1n \u0111\u00e3 bi\u1ebft nh\u1eefng \u0111i\u1ec1u c\u01a1 b\u1ea3n, h\u00e3y xem c\u00e1ch s\u1eed d\u1ee5ng \u0111i\u1ec1u n\u00e0y \u0111\u1ec3 \u0111o l\u01b0\u1ee3ng.<\/p>\n
\u0110\u1ec3 \u0111o l\u01b0\u1ee3ng t\u1ed1t, b\u1ea1n c\u1ea7n h\u1ecdc m\u1ed9t s\u1ed1 c\u00e1ch nh\u1ea5t \u0111\u1ecbnh. Nh\u1eefng \u0111i\u1ec1u n\u00e0y bao g\u1ed3m s\u1eed d\u1ee5ng Lu\u1eadt Lambert-Beer, thi\u1ebft l\u1eadp \u0111\u00fang, v\u00e0 chu\u1ea9n b\u1ecb m\u1eabu c\u1ea9n th\u1eadn. Ph\u1ea7n n\u00e0y gi\u1ea3i th\u00edch c\u00e1c b\u01b0\u1edbc n\u00e0y \u0111\u1ec3 c\u00f3 k\u1ebft qu\u1ea3 v\u1eefng ch\u1eafc.<\/p>\n
Lu\u1eadt Lambert-Beer (A = \u03b5lc) r\u1ea5t quan tr\u1ecdng trong vi\u1ec7c \u0111o l\u01b0\u1eddng v\u1eadt li\u1ec7u. \u0110\u00e2y l\u00e0 c\u00e1ch n\u00f3 \u0111i:<\/p>\n
Lu\u1eadt n\u00e0y cho ch\u00fang ta bi\u1ebft kh\u1ea3 n\u0103ng h\u1ea5p th\u1ee5 t\u0103ng l\u00ean v\u1edbi nhi\u1ec1u v\u1eadt li\u1ec7u h\u01a1n ho\u1eb7c m\u1ed9t con \u0111\u01b0\u1eddng d\u00e0i h\u01a1n. N\u1ebfu b\u1ea1n bi\u1ebft \u03b5\u03b5 v\u00e0 l, b\u1ea1n c\u00f3 th\u1ec3 t\u00ecm th\u1ea5y c t\u1eeb \u0111\u1ed9 h\u1ea5p th\u1ee5. \u0110i\u1ec1u n\u00e0y cho ph\u00e9p b\u1ea1n \u0111o l\u01b0\u1ee3ng ch\u00ednh x\u00e1c.<\/p>\n
\u0110i\u1ec1u chu\u1ea9n \u0111\u1ea3m b\u1ea3o k\u1ebft qu\u1ea3 c\u1ee7a b\u1ea1n l\u00e0 \u0111\u00fang. N\u00f3 c\u00f3 ngh\u0129a l\u00e0 ki\u1ec3m tra m\u00e1y v\u1edbi c\u00e1c m\u1eabu \u0111\u00e3 bi\u1ebft. B\u1ea1n c\u1ea7n t\u1ea1o ra m\u1ed9t bi\u1ec3u \u0111\u1ed3 hi\u1ec7u chu\u1ea9n v\u00e0 s\u1eed d\u1ee5ng c\u00e1c gi\u1ea3i ph\u00e1p ti\u00eau chu\u1ea9n.<\/p>\n
M\u1ed9t bi\u1ec3u \u0111\u1ed3 hi\u1ec7u chu\u1ea9n cho th\u1ea5y \u0111\u1ed9 h\u1ea5p th\u1ee5 so v\u1edbi c\u00e1c l\u01b0\u1ee3ng \u0111\u00e3 bi\u1ebft. \u0110\u00e2y l\u00e0 c\u00e1ch l\u00e0m \u0111i\u1ec1u \u0111\u00f3:<\/p>\n
C\u00e1c gi\u1ea3i ph\u00e1p ti\u00eau chu\u1ea9n ph\u1ea3i s\u1ea1ch s\u1ebd v\u00e0 \u1ed5n \u0111\u1ecbnh. Ch\u00fang c\u1ea7n ph\u00f9 h\u1ee3p v\u1edbi h\u1ed7n h\u1ee3p c\u1ee7a m\u1eabu \u0111\u1ec3 tr\u00e1nh l\u1ed7i. Ki\u1ec3m tra ch\u00fang th\u01b0\u1eddng xuy\u00ean \u0111\u1ec3 gi\u1eef \u0111\u1ed3 h\u1ecda c\u1ee7a b\u1ea1n \u0111\u00fang, v\u00ec m\u00e1y c\u00f3 th\u1ec3 tr\u00f4i ho\u1eb7c c\u00e0i \u0111\u1eb7t th\u1eed nghi\u1ec7m thay \u0111\u1ed5i.<\/p>\n
S\u1eb5n s\u00e0ng m\u1eabu \u0111\u00fang l\u00e0 ch\u00eca kh\u00f3a cho k\u1ebft qu\u1ea3 t\u1ed1t. D\u01b0\u1edbi \u0111\u00e2y l\u00e0 m\u1ed9t s\u1ed1 l\u1eddi khuy\u00ean:<\/p>\n
Ph\u1ed5 \u0111o UV Vis gi\u00fap t\u00ecm th\u1ea5y l\u01b0\u1ee3ng trong nhi\u1ec1u khu v\u1ef1c. Ph\u1ea7n n\u00e0y cho th\u1ea5y c\u00e1ch n\u00f3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng trong ki\u1ec3m tra m\u00f4i tr\u01b0\u1eddng, thu\u1ed1c, th\u1ef1c ph\u1ea9m v\u00e0 n\u00f4ng nghi\u1ec7p.<\/p>\n
M\u00e1y quang ph\u1ed5 UV Vis ki\u1ec3m tra nh\u1eefng th\u1ee9 nh\u01b0 nitrat ho\u1eb7c c\u00e1c m\u1ea3nh h\u1eefu c\u01a1 trong n\u01b0\u1edbc th\u1ea3i. \u0110i\u1ec1u n\u00e0y gi\u00fap tu\u00e2n th\u1ee7 c\u00e1c quy t\u1eafc xanh. V\u00ed d\u1ee5: Persee T6U<\/u><\/a>\u00a0l\u00e0 si\u00eau nh\u1ea1y c\u1ea3m. N\u00f3 ph\u00e1t hi\u1ec7n m\u1ed9t l\u01b0\u1ee3ng nh\u1ecf c\u00e1c ch\u1ea5t x\u1ea5u, gi\u00fap v\u1edbi m\u1ee5c ti\u00eau kh\u00f4ng x\u1ea3 ch\u1ea5t l\u1ecfng (ZLD).<\/p>\n Trong y h\u1ecdc, m\u00e1y quang ph\u1ed5 UV Vis ki\u1ec3m tra DNA, RNA ho\u1eb7c protein \u1edf 260 nm ho\u1eb7c 280 nm. N\u00f3 c\u0169ng ki\u1ec3m tra xem ma t\u00fay v\u1eabn t\u1ed1t hay kh\u00f4ng. N\u00f3 l\u00e0 ch\u00eca kh\u00f3a cho ki\u1ec3m tra ch\u1ea5t l\u01b0\u1ee3ng v\u00e0 quy t\u1eafc. Persee c\u1ee7a T7<\/u><\/a>\u00a0c\u00f3 t\u00ednh n\u0103ng t\u1ef1 \u0111\u1ed9ng cho c\u00e1c th\u1eed nghi\u1ec7m nhanh ch\u00f3ng v\u00e0 l\u1edbn.<\/p>\n M\u00e1y quang ph\u1ed5 UV Vis gi\u1eef cho th\u1ef1c ph\u1ea9m an to\u00e0n b\u1eb1ng c\u00e1ch ki\u1ec3m tra ch\u1ea5t ph\u1ee5 gia ho\u1eb7c ch\u1ea5t x\u1ea5u. N\u00f3 \u0111o c\u00e1c th\u1ee9 nh\u01b0 phenol trong r\u01b0\u1ee3u vang ho\u1eb7c caffeine trong \u0111\u1ed3 u\u1ed1ng. C\u00e1c c\u00f4ng c\u1ee5 c\u1ee7a Persee cung c\u1ea5p k\u1ebft qu\u1ea3 nhanh ch\u00f3ng cho ki\u1ec3m tra ch\u1ea5t l\u01b0\u1ee3ng.<\/p>\n Trong n\u00f4ng nghi\u1ec7p, UV Vis ki\u1ec3m tra ch\u1ea5t dinh d\u01b0\u1ee1ng \u0111\u1ea5t ho\u1eb7c c\u00e1c m\u1ea3nh thu\u1ed1c tr\u1eeb s\u00e2u. N\u00f3 gi\u00fap n\u00f4ng d\u00e2n s\u1eed d\u1ee5ng ph\u00e2n b\u00f3n t\u1ed1t h\u01a1n. C\u00e1c m\u00e1y quang ph\u1ed5 m\u1ea1nh m\u1ebd c\u1ee7a Persee ho\u1ea1t \u0111\u1ed9ng t\u1ed1t trong c\u00e1c l\u0129nh v\u1ef1c.<\/p>\n Nh\u1eefng c\u00e1ch s\u1eed d\u1ee5ng n\u00e0y cho th\u1ea5y quang ph\u1ed5 UV Vis linh ho\u1ea1t nh\u01b0 th\u1ebf n\u00e0o. Nh\u01b0ng \u0111\u1ec3 gi\u1eef cho n\u00f3 ho\u1ea1t \u0111\u1ed9ng t\u1ed1t, b\u1ea1n c\u1ea7n ch\u0103m s\u00f3c v\u00e0 c\u00e1c c\u00f4ng c\u1ee5 ph\u00f9 h\u1ee3p.<\/p>\n \u0110\u1ec3 gi\u1eef cho m\u00e1y quang ph\u1ed5 UV Vis ho\u1ea1t \u0111\u1ed9ng tr\u01a1n tru, b\u1ea1n c\u1ea7n ch\u0103m s\u00f3c th\u01b0\u1eddng xuy\u00ean v\u00e0 c\u00e1c c\u00f4ng c\u1ee5 t\u1ed1t. Ph\u1ea7n n\u00e0y cung c\u1ea5p c\u00e1c l\u1eddi khuy\u00ean v\u00e0 l\u1eddi khuy\u00ean ti\u1ec7n \u00edch.<\/p>\n Ch\u0103m s\u00f3c th\u01b0\u1eddng xuy\u00ean gi\u1eef cho m\u00e1y c\u1ee7a b\u1ea1n \u1ed5n \u0111\u1ecbnh. H\u00e3y th\u1eed c\u00e1c b\u01b0\u1edbc n\u00e0y:<\/p>\n S\u1ed5 tay T6U v\u00e0 T7 c\u1ee7a Persee c\u00f3 k\u1ebf ho\u1ea1ch ch\u0103m s\u00f3c r\u00f5 r\u00e0ng \u0111\u1ec3 l\u00e0m cho n\u00f3 d\u1ec5 d\u00e0ng.<\/p>\n C\u00e1c c\u00f4ng c\u1ee5 t\u1ed1t l\u00e0m cho c\u00e1c b\u00e0i ki\u1ec3m tra t\u1ed1t h\u01a1n v\u00e0 d\u1ec5 d\u00e0ng h\u01a1n.<\/p>\n Cuvettes c\u1ea7n ph\u00f9 h\u1ee3p v\u1edbi m\u1eabu v\u00e0 ph\u1ea1m vi \u00e1nh s\u00e1ng. Quartz l\u00e0m vi\u1ec7c cho \u00e1nh s\u00e1ng UV. Th\u1ee7y tinh l\u00e0 t\u1ed1t cho \u00e1nh s\u00e1ng nh\u00ecn th\u1ea5y. Persee c\u00f3 r\u1ea5t nhi\u1ec1u cuvettes cho c\u00e1c c\u00f4ng vi\u1ec7c kh\u00e1c nhau.<\/p>\n \u0110\u00e8n deuterium cung c\u1ea5p \u00e1nh s\u00e1ng UV \u1ed5n \u0111\u1ecbnh. \u0110\u00e8n vonfram bao ph\u1ee7 \u00e1nh s\u00e1ng c\u00f3 th\u1ec3 nh\u00ecn th\u1ea5y. \u0110\u00e8n T6U v\u00e0 T7 c\u1ee7a Persee k\u00e9o d\u00e0i l\u00e2u, ti\u1ebft ki\u1ec7m ti\u1ec1n cho vi\u1ec7c trao \u0111\u1ed5i.<\/p>\n Gi\u1eef m\u00e1y quang ph\u1ed5 c\u1ee7a b\u1ea1n \u1edf h\u00ecnh d\u1ea1ng h\u00e0ng \u0111\u1ea7u mang l\u1ea1i k\u1ebft qu\u1ea3 tuy\u1ec7t v\u1eddi. B\u00e2y gi\u1edd, h\u00e3y n\u00f3i v\u1ec1 Persee, m\u1ed9t c\u00f4ng ty b\u1ea1n c\u00f3 th\u1ec3 tin t\u01b0\u1edfng.<\/p>\n
<\/p>\n\u1ee8ng d\u1ee5ng D\u01b0\u1ee3c ph\u1ea9m v\u00e0 Khoa h\u1ecdc S\u1ed1ng<\/h3>\n
<\/p>\nKi\u1ec3m so\u00e1t ch\u1ea5t l\u01b0\u1ee3ng ng\u00e0nh c\u00f4ng nghi\u1ec7p th\u1ef1c ph\u1ea9m v\u00e0 \u0111\u1ed3 u\u1ed1ng<\/h3>\n
Th\u1eed nghi\u1ec7m n\u00f4ng nghi\u1ec7p v\u00e0 ph\u00e2n t\u00edch \u0111\u1ea5t<\/h3>\n
T\u1ed1i \u01b0u h\u00f3a hi\u1ec7u su\u1ea5t c\u1ee7a UV Vis Spectrometer<\/h2>\n
M\u1eb9o b\u1ea3o tr\u00ec v\u00e0 kh\u1eafc ph\u1ee5c s\u1ef1 c\u1ed1<\/h3>\n
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Ch\u1ecdn Ph\u1ee5 ki\u1ec7n Ph\u00f9 h\u1ee3p<\/h3>\n
Cuvettes v\u00e0 ng\u01b0\u1eddi gi\u1eef m\u1eabu<\/h4>\n
Ngu\u1ed3n \u00e1nh s\u00e1ng: \u0110\u00e8n Deuterium v\u00e0 Tungsten<\/h4>\n
Persee: M\u1ed9t nh\u00e0 cung c\u1ea5p \u0111\u00e1ng tin c\u1eady c\u1ee7a m\u00e1y quang ph\u1ed5 UV Vis ch\u1ea5t l\u01b0\u1ee3ng cao<\/h2>\n