{"id":3510,"date":"2025-05-01T11:50:18","date_gmt":"2025-05-01T03:50:18","guid":{"rendered":"https:\/\/www.pgeneral.com\/?p=3510"},"modified":"2025-04-27T11:25:39","modified_gmt":"2025-04-27T03:25:39","slug":"what-is-the-difference-between-size-exclusion-chromatography-and-hplc","status":"publish","type":"post","link":"https:\/\/www.pgeneral.com\/vi\/news\/what-is-the-difference-between-size-exclusion-chromatography-and-hplc","title":{"rendered":"S\u1ef1 kh\u00e1c bi\u1ec7t gi\u1eefa s\u1eafc k\u00fd lo\u1ea1i tr\u1eeb k\u00edch th\u01b0\u1edbc v\u00e0 HPLC l\u00e0 g\u00ec?"},"content":{"rendered":"

\"S\u1eafc<\/p>\n

Khi ph\u00e2n t\u00edch c\u00e1c ph\u00e2n t\u1eed trong ph\u00f2ng th\u00ed nghi\u1ec7m, vi\u1ec7c ch\u1ecdn ph\u01b0\u01a1ng ph\u00e1p s\u1eafc k\u00fd th\u00edch h\u1ee3p l\u00e0 r\u1ea5t quan tr\u1ecdng \u0111\u1ec3 c\u00f3 \u0111\u01b0\u1ee3c k\u1ebft qu\u1ea3 \u0111\u00e1ng tin c\u1eady.<\/p>\n

Hi\u1ec3u v\u1ec1 ph\u01b0\u01a1ng ph\u00e1p s\u1eafc k\u00fd c\u01a1 b\u1ea3n<\/h2>\n

Tr\u01b0\u1edbc khi kh\u00e1m ph\u00e1 c\u00e1c chi ti\u1ebft c\u1ee5 th\u1ec3 c\u1ee7a SEC v\u00e0 HPLC, n\u00f3 r\u1ea5t h\u1eefu \u00edch \u0111\u1ec3 hi\u1ec3u c\u00e1c kh\u00e1i ni\u1ec7m c\u1ed1t l\u00f5i c\u1ee7a s\u1eafc k\u00fd.<\/p>\n

X\u00e1c \u0111\u1ecbnh s\u1eafc k\u00fd lo\u1ea1i tr\u1eeb k\u00edch th\u01b0\u1edbc (SEC)<\/h3>\n

Sec, c\u00f2n \u0111\u01b0\u1ee3c g\u1ecdi l\u00e0 l\u1ecdc gel ho\u1eb7c s\u1eafc k\u00fd th\u1ea9m th\u1ea5u gel, l\u00e0 m\u1ed9t ph\u01b0\u01a1ng ph\u00e1p b\u1ea1n s\u1eed d\u1ee5ng \u0111\u1ec3 t\u00e1ch c\u00e1c ph\u00e2n t\u1eed theo k\u00edch th\u01b0\u1edbc.<\/p>\n

X\u00e1c \u0111\u1ecbnh s\u1eafc k\u00fd l\u1ecfng hi\u1ec7u su\u1ea5t cao (HPLC)<\/h3>\n

HPLC l\u00e0 m\u1ed9t k\u1ef9 thu\u1eadt s\u1eafc k\u00fd linh ho\u1ea1t m\u00e0 b\u1ea1n \u00e1p d\u1ee5ng cho c\u00e1c h\u1ee3p ch\u1ea5t ri\u00eang bi\u1ec7t d\u1ef1a tr\u00ean c\u00e1c thu\u1ed9c t\u00ednh nh\u01b0 ph\u00e2n c\u1ef1c ho\u1eb7c \u0111i\u1ec7n t\u00edch.<\/p>\n

C\u01a1 ch\u1ebf v\u00e0 nguy\u00ean t\u1eafc c\u1ed1t l\u00f5i<\/h2>\n

\u0110\u1ec3 n\u1eafm b\u1eaft m\u1ee9c \u0111\u1ed9 kh\u00e1c nhau c\u1ee7a SEC v\u00e0 HPLC, b\u1ea1n ph\u1ea3i ki\u1ec3m tra c\u00e1c c\u01a1 ch\u1ebf c\u01a1 b\u1ea3n c\u1ee7a ch\u00fang.<\/p>\n

C\u00e1ch SEC t\u00e1ch c\u00e1c ph\u00e2n t\u1eed theo k\u00edch th\u01b0\u1edbc<\/h3>\n

Trong Sec, b\u1ea1n s\u1eed d\u1ee5ng m\u1ed9t giai \u0111o\u1ea1n \u0111\u1ee9ng y\u00ean x\u1ed1p.<\/p>\n

C\u00e1ch HPLC \u0111\u1ea1t \u0111\u01b0\u1ee3c s\u1ef1 t\u00e1ch bi\u1ec7t<\/h3>\n

HPLC ph\u00e2n t\u00e1ch c\u00e1c ph\u00e2n t\u1eed d\u1ef1a tr\u00ean c\u00e1c t\u01b0\u01a1ng t\u00e1c h\u00f3a h\u1ecdc v\u1edbi pha t\u0129nh.<\/p>\n

S\u1ef1 kh\u00e1c bi\u1ec7t ch\u00ednh trong ph\u01b0\u01a1ng ph\u00e1p lu\u1eadn<\/h2>\n

C\u00e1c ph\u01b0\u01a1ng ph\u00e1p c\u1ee7a SEC v\u00e0 HPLC x\u00e1c \u0111\u1ecbnh \u0111\u1ed9 ch\u00ednh x\u00e1c v\u00e0 s\u1ef1 ph\u00f9 h\u1ee3p c\u1ee7a ch\u00fang \u0111\u1ed1i v\u1edbi c\u00e1c nhi\u1ec7m v\u1ee5.<\/p>\n

Giai \u0111o\u1ea1n \u0111\u1ee9ng y\u00ean v\u00e0 giai \u0111o\u1ea1n di \u0111\u1ed9ng trong SEC<\/h3>\n

Trong Sec, b\u1ea1n th\u01b0\u1eddng s\u1eed d\u1ee5ng m\u1ed9t giai \u0111o\u1ea1n \u0111\u1ee9ng y\u00ean c\u1ee7a c\u00e1c h\u1ea1t x\u1ed1p, nh\u01b0 agarose ho\u1eb7c silica.<\/p>\n

Giai \u0111o\u1ea1n \u0111\u1ee9ng y\u00ean v\u00e0 giai \u0111o\u1ea1n di \u0111\u1ed9ng trong HPLC<\/h3>\n

HPLC cung c\u1ea5p c\u00e1c giai \u0111o\u1ea1n c\u1ed1 \u0111\u1ecbnh kh\u00e1c nhau.<\/p>\n

C\u00e1c \u1ee9ng d\u1ee5ng v\u00e0 ph\u00e2n t\u00edch ch\u00ednh x\u00e1c<\/h2>\n

C\u00e1c \u1ee9ng d\u1ee5ng c\u1ee7a SEC v\u00e0 HPLC l\u00e0m n\u1ed5i b\u1eadt \u0111i\u1ec3m m\u1ea1nh c\u1ee7a ch\u00fang trong ph\u00e2n t\u00edch ch\u00ednh x\u00e1c.<\/p>\n

Vai tr\u00f2 c\u1ee7a SEC trong ph\u00e2n t\u00edch ph\u00e2n t\u1eed sinh h\u1ecdc<\/h3>\n

SEC l\u00e0 m\u1ed9t ph\u01b0\u01a1ng ph\u00e1p \u01b0a th\u00edch \u0111\u1ec3 ph\u00e2n t\u00edch c\u00e1c ph\u00e2n t\u1eed sinh h\u1ecdc l\u1edbn.<\/p>\n

T\u00ednh linh ho\u1ea1t c\u1ee7a HPLC trong c\u00e1c l\u0129nh v\u1ef1c kh\u00e1c nhau<\/h3>\n

HPLC v\u01b0\u1ee3t tr\u1ed9i trong c\u00e1c \u1ee9ng d\u1ee5ng t\u1eeb ph\u00e1t tri\u1ec3n thu\u1ed1c \u0111\u1ebfn an to\u00e0n th\u1ef1c ph\u1ea9m.<\/p>\n

C\u00e1c y\u1ebfu t\u1ed1 hi\u1ec7u su\u1ea5t<\/h2>\n

\u0110\u1ed9 ch\u00ednh x\u00e1c trong s\u1eafc k\u00fd ph\u1ee5 thu\u1ed9c v\u00e0o c\u00e1c y\u1ebfu t\u1ed1 nh\u01b0 \u0111\u1ed9 ph\u00e2n gi\u1ea3i v\u00e0 \u0111\u1ed9 nh\u1ea1y.<\/p>\n

\u0110\u1ed9 ph\u00e2n gi\u1ea3i v\u00e0 \u0111\u1ed9 nh\u1ea1y trong Sec<\/h3>\n

SEC cung c\u1ea5p \u0111\u1ed9 ph\u00e2n gi\u1ea3i v\u1eefng ch\u1eafc \u0111\u1ec3 t\u00e1ch c\u00e1c ph\u00e2n t\u1eed theo k\u00edch th\u01b0\u1edbc.<\/p>\n

\u0110\u1ed9 ph\u00e2n gi\u1ea3i v\u00e0 \u0111\u1ed9 nh\u1ea1y trong HPLC<\/h3>\n

HPLC th\u01b0\u1eddng cung c\u1ea5p \u0111\u1ed9 ph\u00e2n gi\u1ea3i v\u00e0 \u0111\u1ed9 nh\u1ea1y l\u1edbn h\u01a1n.<\/p>\n

C\u00e2n nh\u1eafc th\u1ef1c t\u1ebf<\/h2>\n

Khi trang b\u1ecb ph\u00f2ng th\u00ed nghi\u1ec7m c\u1ee7a b\u1ea1n, c\u00e1c y\u1ebfu t\u1ed1 th\u1ef1c t\u1ebf nh\u01b0 thi\u1ebft b\u1ecb v\u00e0 b\u1ea3o tr\u00ec \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn s\u1ef1 l\u1ef1a ch\u1ecdn k\u1ef9 thu\u1eadt c\u1ee7a b\u1ea1n.<\/p>\n

Thi\u1ebft b\u1ecb v\u00e0 thi\u1ebft l\u1eadp cho Sec<\/h3>\n

\u0110\u1ed1i v\u1edbi Sec, b\u1ea1n c\u1ea7n m\u1ed9t h\u1ec7 th\u1ed1ng v\u1edbi m\u1ed9t c\u1ed9t h\u1ea1t x\u1ed1p.<\/p>\n

Thi\u1ebft b\u1ecb v\u00e0 thi\u1ebft l\u1eadp cho HPLC<\/h3>\n

H\u1ec7 th\u1ed1ng HPLC ph\u1ee9c t\u1ea1p h\u01a1n.<\/p>\n

\u01afu \u0111i\u1ec3m v\u00e0 h\u1ea1n ch\u1ebf<\/h2>\n

M\u1ed7i k\u1ef9 thu\u1eadt c\u00f3 \u0111i\u1ec3m m\u1ea1nh v\u00e0 \u0111i\u1ec3m y\u1ebfu.<\/p>\n

\u0110i\u1ec3m m\u1ea1nh c\u1ee7a SEC trong ph\u00e2n t\u00edch ch\u00ednh x\u00e1c<\/h3>\n

SEC v\u01b0\u1ee3t tr\u1ed9i trong vi\u1ec7c ph\u00e2n t\u00edch c\u00e1c ph\u00e2n t\u1eed l\u1edbn m\u00e0 kh\u00f4ng c\u00f3 \u0111i\u1ec1u ki\u1ec7n kh\u1eafc nghi\u1ec7t.<\/p>\n

\u0110i\u1ec3m m\u1ea1nh c\u1ee7a HPLC trong ph\u00e2n t\u00edch ch\u00ednh x\u00e1c<\/h3>\n

S\u1ee9c m\u1ea1nh c\u1ee7a HPLC l\u00e0 s\u1ef1 linh ho\u1ea1t v\u00e0 \u0111\u1ed9 nh\u1ea1y cao c\u1ee7a n\u00f3.<\/p>\n

H\u1ea1n ch\u1ebf c\u1ee7a Sec<\/h3>\n

SEC t\u1eadp trung v\u00e0o ph\u00e2n t\u00e1ch d\u1ef1a tr\u00ean k\u00edch th\u01b0\u1edbc.<\/p>\n

H\u1ea1n ch\u1ebf c\u1ee7a HPLC<\/h3>\n

HPLC c\u00f3 th\u1ec3 l\u00e0 th\u00e1ch th\u1ee9c \u0111\u1ec3 t\u1ed1i \u01b0u h\u00f3a.<\/p>\n

Ch\u1ecdn \u0111\u00fang k\u1ef9 thu\u1eadt<\/h2>\n

L\u1ef1a ch\u1ecdn gi\u1eefa SEC v\u00e0 HPLC ph\u1ee5 thu\u1ed9c v\u00e0o c\u00e1c m\u1ee5c ti\u00eau ph\u00e2n t\u00edch c\u1ee7a b\u1ea1n.<\/p>\n

Khi n\u00e0o n\u00ean s\u1eed d\u1ee5ng Sec tr\u00ean HPLC<\/h3>\n

Ch\u1ecdn SEC khi l\u00e0m vi\u1ec7c v\u1edbi c\u00e1c ph\u00e2n t\u1eed sinh h\u1ecdc l\u1edbn.<\/p>\n

Khi HPLC v\u01b0\u1ee3t tr\u1ed9i h\u01a1n Sec<\/h3>\n

Ch\u1ecdn HPLC \u0111\u1ec3 ph\u00e2n t\u00edch c\u00e1c ph\u00e2n t\u1eed nh\u1ecf ho\u1eb7c \u0111\u1ecbnh l\u01b0\u1ee3ng t\u1ea1p ch\u1ea5t.<\/p>\n

PERSEE: M\u1ed9t nh\u00e0 cung c\u1ea5p \u0111\u00e1ng tin c\u1eady cho c\u00e1c gi\u1ea3i ph\u00e1p s\u1eafc k\u00fd lo\u1ea1i tr\u1eeb k\u00edch th\u01b0\u1edbc<\/h2>\n

\"PERSEE\"<\/p>\n

Khi thi\u1ebft l\u1eadp ph\u00f2ng th\u00ed nghi\u1ec7m c\u1ee7a b\u1ea1n cho Gi\u00e2y,<\/u><\/a>\u00a0Ch\u1ecdn m\u1ed9t nh\u00e0 cung c\u1ea5p \u0111\u00e1ng tin c\u1eady l\u00e0 r\u1ea5t quan tr\u1ecdng. PERSEE<\/u><\/a>\u00a0l\u00e0 m\u1ed9t nh\u00e0 cung c\u1ea5p \u0111\u00e1ng tin c\u1eady c\u1ee7a D\u1ee5ng c\u1ee5 ph\u00e2n t\u00edch<\/u><\/a>. H\u1ec7 th\u1ed1ng m\u1ea1nh m\u1ebd<\/u><\/a>.<\/p>\n

C\u00e2u h\u1ecfi th\u01b0\u1eddng g\u1eb7p v\u1ec1 s\u1eafc k\u00fd lo\u1ea1i tr\u1eeb k\u00edch th\u01b0\u1edbc v\u00e0 HPLC<\/h2>\n

Nh\u1eefng lo\u1ea1i m\u1eabu ph\u00f9 h\u1ee3p nh\u1ea5t \u0111\u1ec3 s\u1eafc k\u00fd lo\u1ea1i tr\u1eeb k\u00edch th\u01b0\u1edbc?<\/h3>\n

B\u1ea1n n\u00ean s\u1eed d\u1ee5ng SEC cho c\u00e1c ph\u00e2n t\u1eed sinh h\u1ecdc l\u1edbn.<\/p>\n

HPLC c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 ph\u00e2n t\u00edch ph\u00e2n t\u1eed sinh h\u1ecdc nh\u01b0 Sec kh\u00f4ng?<\/h3>\n

C\u00f3, b\u1ea1n c\u00f3 th\u1ec3 s\u1eed d\u1ee5ng HPLC \u0111\u1ec3 ph\u00e2n t\u00edch ph\u00e2n t\u1eed sinh h\u1ecdc.<\/p>\n

L\u00e0m c\u00e1ch n\u00e0o \u0111\u1ec3 ch\u1ecdn gi\u1eefa SEC v\u00e0 HPLC cho ph\u00f2ng th\u00ed nghi\u1ec7m c\u1ee7a t\u00f4i?<\/h3>\n

H\u00e3y xem x\u00e9t lo\u1ea1i m\u1eabu c\u1ee7a b\u1ea1n v\u00e0 c\u00e1c m\u1ee5c ti\u00eau ph\u00e2n t\u00edch.<\/p>\n

 <\/p>","protected":false},"excerpt":{"rendered":"

When analyzing molecules in a lab, selecting the appropriate chromatography method is crucial for obtaining reliable results. Size exclusion chromatography (SEC) and high-performance liquid chromatography (HPLC) are two commonly used techniques. Each has distinct advantages. Knowing their differences helps you choose the method that suits your analytical objectives. This blog examines how SEC and HPLC […]<\/p>\n","protected":false},"author":1,"featured_media":3511,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[38,37],"tags":[],"class_list":["post-3510","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-industry-information","category-news"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts\/3510","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/comments?post=3510"}],"version-history":[{"count":0,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts\/3510\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/media\/3511"}],"wp:attachment":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/media?parent=3510"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/categories?post=3510"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/tags?post=3510"}],"curies":[{"name":"WP","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}