{"id":3504,"date":"2025-04-25T11:50:19","date_gmt":"2025-04-25T03:50:19","guid":{"rendered":"https:\/\/www.pgeneral.com\/?p=3504"},"modified":"2025-04-24T14:31:42","modified_gmt":"2025-04-24T06:31:42","slug":"what-are-the-factors-affecting-size-exclusion-chromatography","status":"publish","type":"post","link":"https:\/\/www.pgeneral.com\/vi\/news\/what-are-the-factors-affecting-size-exclusion-chromatography","title":{"rendered":"C\u00e1c y\u1ebfu t\u1ed1 \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn s\u1eafc k\u00fd lo\u1ea1i tr\u1eeb k\u00edch th\u01b0\u1edbc l\u00e0 g\u00ec?"},"content":{"rendered":"

\"S\u1eafc<\/p>\n

S\u1eafc k\u00fd lo\u1ea1i tr\u1eeb k\u00edch th\u01b0\u1edbc (SEC) l\u00e0 m\u1ed9t k\u1ef9 thu\u1eadt ph\u00e2n t\u00edch ph\u1ed5 bi\u1ebfn ph\u00e2n t\u00edch c\u00e1c ph\u00e2n t\u1eed theo k\u00edch th\u01b0\u1edbc, v\u00e0 n\u00f3 l\u00e0 ti\u1ec7n \u00edch l\u1edbn trong khoa h\u1ecdc polymer v\u00e0 h\u00f3a sinh.<\/p>\n

Gi\u1edbi thi\u1ec7u v\u1ec1 s\u1eafc k\u00fd lo\u1ea1i tr\u1eeb k\u00edch th\u01b0\u1edbc<\/h2>\n

Sec, ho\u1eb7c th\u1ea9m th\u1ea5u gel ho\u1eb7c s\u1eafc k\u00fd l\u1ecdc gel, cho ph\u00e9p b\u1ea1n ph\u00e2n \u0111o\u1ea1n c\u00e1c ph\u00e2n t\u1eed theo k\u00edch th\u01b0\u1edbc khi ch\u00fang \u0111i qua m\u1ed9t pha \u0111\u1ee9ng y\u00ean.<\/p>\n

Nguy\u00ean t\u1eafc s\u1eafc k\u00fd lo\u1ea1i tr\u1eeb k\u00edch th\u01b0\u1edbc c\u01a1 b\u1ea3n<\/h2>\n

Tr\u01b0\u1edbc khi b\u1ea1n \u0111\u1ea1t \u0111\u01b0\u1ee3c nh\u1eefng \u1ea3nh h\u01b0\u1edfng tr\u00ean Sec, b\u1ea1n c\u1ea7n bi\u1ebft c\u00e1c nguy\u00ean t\u1eafc c\u01a1 b\u1ea3n c\u1ee7a n\u00f3.<\/p>\n

C\u01a1 ch\u1ebf t\u00e1ch<\/h3>\n

Trong Sec, s\u1ef1 ph\u00e2n t\u00e1ch x\u1ea3y ra khi m\u1eabu c\u1ee7a b\u1ea1n di chuy\u1ec3n qua m\u1ed9t c\u1ed9t \u0111\u01b0\u1ee3c \u0111\u00f3ng g\u00f3i v\u1edbi c\u00e1c h\u1ea1t x\u1ed1p.<\/p>\n

Vai tr\u00f2 c\u1ee7a ph\u01b0\u01a1ng ti\u1ec7n x\u1ed1p<\/h3>\n

C\u00e1c ph\u01b0\u01a1ng ti\u1ec7n x\u1ed1p, th\u01b0\u1eddng l\u00e0 h\u1ea1t ho\u1eb7c gel, l\u00e0 tr\u00e1i tim c\u1ee7a Sec.<\/p>\n

C\u00e1c th\u00e0nh ph\u1ea7n ch\u00ednh c\u1ee7a h\u1ec7 th\u1ed1ng s\u1eafc k\u00fd lo\u1ea1i tr\u1eeb k\u00edch th\u01b0\u1edbc<\/h2>\n

M\u1ed9t h\u1ec7 th\u1ed1ng SEC \u0111\u01b0\u1ee3c t\u1ea1o th\u00e0nh t\u1eeb m\u1ed9t s\u1ed1 th\u00e0nh ph\u1ea7n quan tr\u1ecdng l\u00e0m vi\u1ec7c c\u00f9ng nhau \u0111\u1ec3 \u0111\u1ea1t \u0111\u01b0\u1ee3c s\u1ef1 ph\u00e2n t\u00e1ch hi\u1ec7u qu\u1ea3.<\/p>\n

Thi\u1ebft k\u1ebf c\u1ed9t<\/h3>\n

C\u1ed9t, \u0111\u01b0\u1ee3c \u0111\u00f3ng g\u00f3i v\u1edbi ph\u01b0\u01a1ng ti\u1ec7n x\u1ed1p, l\u00e0 n\u01a1i x\u1ea3y ra s\u1ef1 t\u00e1ch bi\u1ec7t.<\/p>\n

Giai \u0111o\u1ea1n di \u0111\u1ed9ng<\/h3>\n

Pha di \u0111\u1ed9ng, b\u1ed9 \u0111\u1ec7m ho\u1eb7c dung m\u00f4i ch\u1ea5t l\u1ecfng, mang m\u1eabu c\u1ee7a b\u1ea1n th\u00f4ng qua c\u1ed9t.<\/p>\n

H\u1ec7 th\u1ed1ng m\u00e1y d\u00f2<\/h3>\n

M\u00e1y d\u00f2 theo d\u00f5i c\u00e1c h\u1ee3p ch\u1ea5t r\u1eeda gi\u1ea3i, t\u1ea1o d\u1eef li\u1ec7u \u0111\u1ec3 ph\u00e2n t\u00edch.<\/p>\n

C\u00e1c y\u1ebfu t\u1ed1 \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn hi\u1ec7u qu\u1ea3 ph\u00e2n t\u00e1ch<\/h2>\n

M\u1ed9t s\u1ed1 bi\u1ebfn \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn c\u00e1ch SEC th\u1ef1c hi\u1ec7n t\u1ed1t.<\/p>\n

\u0110\u1eb7c \u0111i\u1ec3m c\u1ed9t<\/h3>\n

Thi\u1ebft k\u1ebf c\u1ed9t \u0111\u00f3ng m\u1ed9t vai tr\u00f2 quan tr\u1ecdng trong hi\u1ec7u su\u1ea5t c\u1ee7a SEC.<\/p>\n

Ph\u00e2n ph\u1ed1i k\u00edch th\u01b0\u1edbc l\u1ed7 ch\u00e2n l\u00f4ng<\/h4>\n

K\u00edch th\u01b0\u1edbc l\u1ed7 ch\u00e2n l\u00f4ng c\u1ee7a ph\u01b0\u01a1ng ti\u1ec7n truy\u1ec1n th\u00f4ng c\u1ed9t x\u00e1c \u0111\u1ecbnh c\u00e1c ph\u00e2n t\u1eed n\u00e0o c\u00f3 th\u1ec3 x\u00e2m nh\u1eadp v\u00e0 lo\u1ea1i n\u00e0o \u0111\u01b0\u1ee3c lo\u1ea1i tr\u1eeb.<\/p>\n

K\u00edch th\u01b0\u1edbc h\u1ea1t v\u00e0 t\u00ednh \u0111\u1ed3ng nh\u1ea5t<\/h4>\n

C\u00e1c h\u1ea1t nh\u1ecf h\u01a1n, \u0111\u1ed3ng nh\u1ea5t trong c\u1ed9t c\u1ea3i thi\u1ec7n \u0111\u1ed9 ph\u00e2n gi\u1ea3i b\u1eb1ng c\u00e1ch gi\u1ea3m khu\u1ebfch t\u00e1n.<\/p>\n

Thu\u1ed9c t\u00ednh pha di \u0111\u1ed9ng<\/h3>\n

C\u00e1c \u0111\u1eb7c \u0111i\u1ec3m c\u1ee7a giai \u0111o\u1ea1n di \u0111\u1ed9ng \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn c\u00e1ch m\u1eabu c\u1ee7a b\u1ea1n ho\u1ea1t \u0111\u1ed9ng trong qu\u00e1 tr\u00ecnh t\u00e1ch.<\/p>\n

\u0110\u1ed9 nh\u1edbt dung m\u00f4i<\/h4>\n

\u0110\u1ed9 nh\u1edbt c\u1ee7a pha di \u0111\u1ed9ng \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn \u0111\u1ed9ng l\u1ef1c d\u00f2ng ch\u1ea3y v\u00e0 t\u1ed1c \u0111\u1ed9 khu\u1ebfch t\u00e1n.<\/p>\n

S\u1ee9c m\u1ea1nh ion<\/h4>\n

S\u1ee9c m\u1ea1nh ion c\u1ee7a pha di \u0111\u1ed9ng c\u00f3 th\u1ec3 \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn c\u00e1c t\u01b0\u01a1ng t\u00e1c ph\u00e2n t\u1eed.<\/p>\n

Thu\u1ed9c t\u00ednh m\u1eabu<\/h3>\n

C\u00e1c thu\u1ed9c t\u00ednh m\u1eabu c\u1ee7a b\u1ea1n t\u00e1c \u0111\u1ed9ng tr\u1ef1c ti\u1ebfp \u0111\u1ebfn k\u1ebft qu\u1ea3 c\u1ee7a SEC.<\/p>\n

K\u00edch th\u01b0\u1edbc v\u00e0 h\u00ecnh d\u1ea1ng ph\u00e2n t\u1eed<\/h4>\n

K\u00edch th\u01b0\u1edbc v\u00e0 h\u00ecnh d\u1ea1ng c\u1ee7a c\u00e1c ph\u00e2n t\u1eed m\u1eabu c\u1ee7a b\u1ea1n x\u00e1c \u0111\u1ecbnh h\u00e0nh vi r\u1eeda gi\u1ea3i c\u1ee7a ch\u00fang.<\/p>\n

N\u1ed3ng \u0111\u1ed9 m\u1eabu<\/h4>\n

N\u1ed3ng \u0111\u1ed9 m\u1eabu cao c\u00f3 th\u1ec3 d\u1eabn \u0111\u1ebfn qu\u00e1 t\u1ea3i c\u1ed9t, gi\u1ea3m \u0111\u1ed9 ph\u00e2n gi\u1ea3i.<\/p>\n

Tham s\u1ed1 ho\u1ea1t \u0111\u1ed9ng<\/h3>\n

C\u00e1ch b\u1ea1n ch\u1ea1y h\u1ec7 th\u1ed1ng SEC \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn hi\u1ec7u su\u1ea5t c\u1ee7a n\u00f3.<\/p>\n

T\u1ed1c \u0111\u1ed9 d\u00f2ng ch\u1ea3y<\/h4>\n

T\u1ed1c \u0111\u1ed9 d\u00f2ng ch\u1ea3y c\u1ee7a pha di \u0111\u1ed9ng \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn th\u1eddi gian ph\u00e2n t\u00e1ch v\u00e0 \u0111\u1ed9 ph\u00e2n gi\u1ea3i.<\/p>\n

Ki\u1ec3m so\u00e1t nhi\u1ec7t \u0111\u1ed9<\/h4>\n

Nhi\u1ec7t \u0111\u1ed9 \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn \u0111\u1ed9 nh\u1edbt c\u1ee7a pha di \u0111\u1ed9ng v\u00e0 \u0111\u1ed9 \u1ed5n \u0111\u1ecbnh m\u1eabu.<\/p>\n

T\u00e1c \u0111\u1ed9ng c\u1ee7a b\u1ea3o tr\u00ec h\u1ec7 th\u1ed1ng<\/h2>\n

Vi\u1ec7c duy tr\u00ec th\u01b0\u1eddng xuy\u00ean h\u1ec7 th\u1ed1ng SEC c\u1ee7a b\u1ea1n l\u00e0 \u0111i\u1ec1u c\u1ea7n thi\u1ebft cho hi\u1ec7u su\u1ea5t \u0111\u00e1ng tin c\u1eady.<\/p>\n

L\u01b0u tr\u1eef v\u00e0 l\u00e0m s\u1ea1ch c\u1ed9t<\/h3>\n

L\u01b0u tr\u1eef v\u00e0 l\u00e0m s\u1ea1ch c\u1ed9t th\u00edch h\u1ee3p ng\u0103n ng\u1eeba \u00f4 nhi\u1ec5m v\u00e0 suy tho\u00e1i.<\/p>\n

Hi\u1ec7u chu\u1ea9n m\u00e1y d\u00f2<\/h3>\n

M\u00e1y d\u00f2 y\u00eau c\u1ea7u hi\u1ec7u chu\u1ea9n \u0111\u1ecbnh k\u1ef3 \u0111\u1ec3 duy tr\u00ec \u0111\u1ed9 ch\u00ednh x\u00e1c.<\/p>\n

C\u00e1c \u1ee9ng d\u1ee5ng c\u1ee7a s\u1eafc k\u00fd lo\u1ea1i tr\u1eeb k\u00edch th\u01b0\u1edbc<\/h2>\n

T\u00ednh linh ho\u1ea1t c\u1ee7a SEC l\u00e0m cho n\u00f3 c\u00f3 gi\u00e1 tr\u1ecb tr\u00ean c\u00e1c l\u0129nh v\u1ef1c kh\u00e1c nhau.<\/p>\n

Ph\u00e2n t\u00edch ph\u00e2n t\u1eed sinh h\u1ecdc<\/h3>\n

Trong h\u00f3a sinh, b\u1ea1n s\u1eed d\u1ee5ng SEC \u0111\u1ec3 tinh ch\u1ebf v\u00e0 m\u00f4 t\u1ea3 c\u00e1c protein, axit nucleic v\u00e0 c\u00e1c ph\u00e2n t\u1eed sinh h\u1ecdc kh\u00e1c, h\u1ed7 tr\u1ee3 nghi\u00ean c\u1ee9u v\u00e0 ph\u00e1t tri\u1ec3n \u0111i\u1ec1u tr\u1ecb.<\/p>\n

\u0110\u1eb7c t\u00ednh polymer<\/h3>\n

SEC gi\u00fap b\u1ea1n ph\u00e2n t\u00edch c\u00e1c polyme b\u1eb1ng c\u00e1ch x\u00e1c \u0111\u1ecbnh ph\u00e2n ph\u1ed1i tr\u1ecdng l\u01b0\u1ee3ng ph\u00e2n t\u1eed c\u1ee7a ch\u00fang, h\u1ed7 tr\u1ee3 ph\u00e1t tri\u1ec3n v\u1eadt li\u1ec7u v\u00e0 ki\u1ec3m so\u00e1t ch\u1ea5t l\u01b0\u1ee3ng.<\/p>\n

Nghi\u00ean c\u1ee9u m\u00f4i tr\u01b0\u1eddng<\/h3>\n

B\u1ea1n s\u1eed d\u1ee5ng SEC \u0111\u1ec3 nghi\u00ean c\u1ee9u c\u00e1c m\u1eabu m\u00f4i tr\u01b0\u1eddng ph\u1ee9c t\u1ea1p, ch\u1eb3ng h\u1ea1n nh\u01b0 c\u00e1c ch\u1ea5t humic, \u0111\u1ec3 hi\u1ec3u th\u00e0nh ph\u1ea7n v\u00e0 h\u00e0nh vi c\u1ee7a ch\u00fang trong c\u00e1c h\u1ec7 sinh th\u00e1i.<\/p>\n

Nh\u1eefng ti\u1ebfn b\u1ed9 trong c\u00f4ng ngh\u1ec7 s\u1eafc k\u00fd lo\u1ea1i tr\u1eeb k\u00edch th\u01b0\u1edbc<\/h2>\n

SEC ti\u1ebfp t\u1ee5c ph\u00e1t tri\u1ec3n, v\u1edbi nh\u1eefng \u0111\u1ed5i m\u1edbi c\u1ea3i thi\u1ec7n hi\u1ec7u qu\u1ea3 v\u00e0 kh\u1ea3 n\u0103ng s\u1eed d\u1ee5ng c\u1ee7a n\u00f3.<\/p>\n

C\u1ed9t \u0111\u1ed9 ph\u00e2n gi\u1ea3i cao<\/h3>\n

C\u00e1c c\u1ed9t m\u1edbi h\u01a1n v\u1edbi c\u00e1c h\u1ea1t m\u1ecbn h\u01a1n v\u00e0 c\u1ea5u tr\u00fac l\u1ed7 r\u1ed7ng \u0111\u01b0\u1ee3c t\u1ed1i \u01b0u h\u00f3a t\u0103ng c\u01b0\u1eddng \u0111\u1ed9 ph\u00e2n gi\u1ea3i.<\/p>\n

H\u1ec7 th\u1ed1ng t\u1ef1 \u0111\u1ed9ng<\/h3>\n

T\u1ef1 \u0111\u1ed9ng h\u00f3a trong h\u1ec7 th\u1ed1ng SEC h\u1ee3p l\u00fd h\u00f3a quy tr\u00ecnh c\u00f4ng vi\u1ec7c v\u00e0 gi\u1ea3m l\u1ed7i th\u1ee7 c\u00f4ng.<\/p>\n

PERSEE: Nh\u00e0 cung c\u1ea5p s\u1eafc k\u00fd lo\u1ea1i tr\u1eeb k\u00edch th\u01b0\u1edbc \u0111\u00e1ng tin c\u1eady<\/h2>\n

\"PERSEE\"<\/p>\n

Khi trang b\u1ecb ph\u00f2ng th\u00ed nghi\u1ec7m c\u1ee7a b\u1ea1n cho Sec, b\u1ea1n c\u1ea7n c\u00e1c c\u00f4ng c\u1ee5 v\u00e0 h\u1ed7 tr\u1ee3 \u0111\u00e1ng tin c\u1eady<\/u><\/a>. PERSEE<\/u><\/a>\u00a0General Compass Co., Ltd., m\u1ed9t doanh nghi\u1ec7p c\u00f4ng ngh\u1ec7 cao h\u00e0ng \u0111\u1ea7u, chuy\u00ean v\u1ec1 c\u00e1c c\u00f4ng c\u1ee5 ph\u00e2n t\u00edch, bao g\u1ed3m c\u00e1c gi\u1ea3i ph\u00e1p s\u1eafc k\u00fd ti\u00ean ti\u1ebfn. H\u1ec7 th\u1ed1ng SEC ch\u1ea5t l\u01b0\u1ee3ng cao<\/u><\/a>\u00a0\u0111\u01b0\u1ee3c thi\u1ebft k\u1ebf cho \u0111\u1ed9 ch\u00ednh x\u00e1c v\u00e0 \u0111\u1ed9 tin c\u1eady. m\u1ed9t \u0111\u1ed1i t\u00e1c \u0111\u00e1ng tin c\u1eady<\/u><\/a>\u00a0cho nhu c\u1ea7u ph\u00f2ng th\u00ed nghi\u1ec7m c\u1ee7a b\u1ea1n.<\/p>\n

C\u00e2u h\u1ecfi th\u01b0\u1eddng g\u1eb7p<\/h2>\n

L\u00e0m th\u1ebf n\u00e0o \u0111\u1ec3 b\u1ea1n ch\u1ecdn c\u1ed9t ph\u00f9 h\u1ee3p \u0111\u1ec3 s\u1eafc k\u00fd lo\u1ea1i tr\u1eeb k\u00edch th\u01b0\u1edbc?<\/h3>\n

B\u1ea1n ch\u1ecdn m\u1ed9t c\u1ed9t d\u1ef1a tr\u00ean ph\u1ea1m vi tr\u1ecdng l\u01b0\u1ee3ng ph\u00e2n t\u1eed m\u1eabu c\u1ee7a b\u1ea1n v\u00e0 \u0111\u1ed9 ph\u00e2n gi\u1ea3i mong mu\u1ed1n.<\/p>\n

T\u1ea1i sao pha di \u0111\u1ed9ng quan tr\u1ecdng trong Sec?<\/h3>\n

Pha di \u0111\u1ed9ng \u1ed5n \u0111\u1ecbnh m\u1eabu c\u1ee7a b\u1ea1n v\u00e0 \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn s\u1ef1 t\u01b0\u01a1ng t\u00e1c c\u1ee7a n\u00f3 v\u1edbi giai \u0111o\u1ea1n \u0111\u1ee9ng y\u00ean.<\/p>\n

\u0110i\u1ec1u g\u00ec x\u1ea3y ra n\u1ebfu b\u1ea1n s\u1eed d\u1ee5ng n\u1ed3ng \u0111\u1ed9 m\u1eabu qu\u00e1 cao trong Sec?<\/h3>\n

N\u1ed3ng \u0111\u1ed9 m\u1eabu cao c\u00f3 th\u1ec3 l\u00e0m qu\u00e1 t\u1ea3i c\u1ed9t, d\u1eabn \u0111\u1ebfn c\u00e1c \u0111\u1ec9nh r\u1ed9ng h\u01a1n v\u00e0 gi\u1ea3m \u0111\u1ed9 ph\u00e2n gi\u1ea3i.<\/p>\n

 <\/p>","protected":false},"excerpt":{"rendered":"

Size Exclusion Chromatography (SEC) is a common analytical technique that analyzes molecules by size, and it is of great utility in polymer science and biochemistry. If you are researching how to optimize your SEC experiments, you need to be familiar with variables that influence its performance. This tutorial guides you through the principles, the equipment, […]<\/p>\n","protected":false},"author":1,"featured_media":3505,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[38,37],"tags":[],"class_list":["post-3504","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-industry-information","category-news"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts\/3504","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/comments?post=3504"}],"version-history":[{"count":0,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts\/3504\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/media\/3505"}],"wp:attachment":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/media?parent=3504"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/categories?post=3504"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/tags?post=3504"}],"curies":[{"name":"WP","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}