{"id":2799,"date":"2023-09-14T13:29:59","date_gmt":"2023-09-14T05:29:59","guid":{"rendered":"http:\/\/www.pgeneral.com\/?p=2799"},"modified":"2023-09-14T13:30:06","modified_gmt":"2023-09-14T05:30:06","slug":"method-for-the-determination-of-diastase-number-in-honey-by-uv-vis-spectrophotometer","status":"publish","type":"post","link":"https:\/\/www.pgeneral.com\/vi\/applications\/food-and-drugs\/method-for-the-determination-of-diastase-number-in-honey-by-uv-vis-spectrophotometer","title":{"rendered":"Ph\u01b0\u01a1ng ph\u00e1p x\u00e1c \u0111\u1ecbnh s\u1ed1 l\u01b0\u1ee3ng diastase trong m\u1eadt ong b\u1eb1ng m\u00e1y quang ph\u1ed5 UV-Vis"},"content":{"rendered":"

Nguy\u00ean t\u1eafc
\nSau khi \u0111\u1eb7t dung d\u1ecbch tinh b\u1ed9t v\u00e0o dung d\u1ecbch m\u1eabu m\u1eadt ong, m\u1ed9t s\u1ed1 tinh b\u1ed9t l\u00e0 th\u1ee7y ph\u00e2n b\u1eb1ng diastase trong m\u1eadt ong.<\/p>\n

Thu\u1ed1c th\u1eed v\u00e0 v\u1eadt li\u1ec7u
\nNgo\u1ea1i tr\u1eeb nh\u1eefng ng\u01b0\u1eddi c\u00f3 th\u00f4ng b\u00e1o c\u1ee5 th\u1ec3, thu\u1ed1c th\u1eed \u0111\u1ed9 AR \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng;<\/p>\n

Thu\u1ed1c th\u1eed:
\n1. I\u1ed1t.
\n2. Kali iodide.
\n3. Natri acetate (CH3coona \u00b7 3H2O).
\n4. Axit acetate \u0111\u00e1
\n5. Natri clorua.
\n6. Tinh b\u1ed9t h\u00f2a tan.
\n7. Gi\u1ea3i ph\u00e1p ch\u1ee9ng kho\u00e1n i\u1ed1t: n\u1eb7ng 8,8g i\u1ed1t v\u00e0 22g kali iodide, tr\u1ed9n v\u1edbi 30ml ~ 40ml n\u01b0\u1edbc, pha lo\u00e3ng \u0111\u1ebfn 1000ml.
\n8. Dung d\u1ecbch i\u1ed1t: C\u00e2n 20g kali i\u1ed1t, th\u00eam dung d\u1ecbch i\u1ed1t 5ml, pha lo\u00e3ng \u0111\u1ebfn 500ml b\u1eb1ng n\u01b0\u1edbc (dung d\u1ecbch n\u00e0y ch\u1ec9 c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng trong 2 ng\u00e0y).
\n9. Dung d\u1ecbch \u0111\u1ec7m axetat: pH 5,3 (1,59 mol\/L). C\u00e2n 87g Natri axetat v\u00e0 pha lo\u00e3ng trong 400mL n\u01b0\u1edbc. Th\u00eam 10,5mL axit axetat \u0111\u00e1 v\u00e0 pha lo\u00e3ng \u0111\u1ebfn 500mL. N\u1ebfu c\u1ea7n, s\u1eed d\u1ee5ng natri axetat ho\u1eb7c axit axetat \u0111\u1ec3 \u0111i\u1ec1u ch\u1ec9nh gi\u00e1 tr\u1ecb pH \u0111\u1ebfn 5,3.
\n10. Dung d\u1ecbch natri clorua (0,5 mol\/L): c\u00e2n 14,5g NaCl, h\u00f2a tan trong 500mL n\u01b0\u1edbc.
\n11. Dung d\u1ecbch tinh b\u1ed9t: H\u00f2a tan tinh b\u1ed9t h\u00f2a tan 2G trong n\u01b0\u1edbc 90ml.<\/p>\n

Thi\u1ebft b\u1ecb:
\n1. M\u00e1y quang ph\u1ed5 UV-Vis.
\n2. Gi\u00e1 \u0111\u1ee1 t\u1ebf b\u00e0o nhi\u1ec7t \u0111\u1ed9 kh\u00f4ng \u0111\u1ed5i<\/p>\n

Chu\u1ea9n b\u1ecb v\u00e0 b\u1ea3o qu\u1ea3n m\u1eabu
\nChu\u1ea9n b\u1ecb m\u1eabu:
\nKhu\u1ea5y t\u1ed1t m\u1eabu, sau \u0111\u00f3 chuy\u1ec3n 0,5kg v\u00e0o chai m\u1eabu d\u01b0\u1edbi d\u1ea1ng m\u1eabu th\u1eed nghi\u1ec7m.
\nL\u01afU \u00dd: Cho d\u00f9 tinh th\u1ec3 xu\u1ea5t hi\u1ec7n hay kh\u00f4ng, t\u1ea5t c\u1ea3 c\u00e1c m\u1eabu kh\u00f4ng th\u1ec3 \u0111\u01b0\u1ee3c l\u00e0m n\u00f3ng.<\/p>\n

B\u1ea3o qu\u1ea3n m\u1eabu:
\nM\u1eabu c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c b\u1ea3o qu\u1ea3n d\u01b0\u1edbi nhi\u1ec7t \u0111\u1ed9 ph\u00f2ng.<\/p>\n

Th\u1ee7 t\u1ee5c th\u1eed nghi\u1ec7m
\nKi\u1ec3m tra ti\u00eau chu\u1ea9n c\u1ee7a dung d\u1ecbch tinh b\u1ed9t
\n\u0110o 5,0 ml dung d\u1ecbch tinh b\u1ed9t (thu\u1ed1c th\u1eed 11) v\u00e0 10,0 ml n\u01b0\u1edbc, \u0111\u1eb7t trong 40 \u2103 n\u01b0\u1edbc t\u1eafm ri\u00eang trong 15 ph\u00fat.<\/p>\n

Ki\u1ec3m tra m\u1eabu:
\n1. \u0110i\u1ec1u ki\u1ec7n quang ph\u1ed5 k\u1ebf:
\nA) B\u01b0\u1edbc s\u00f3ng: 660nm
\nb) Tham kh\u1ea3o: N\u01b0\u1edbc
\n2. M\u1eabu ti\u1ec1n x\u1eed l\u00fd: \u0110o 5g (\u00b1 0,01g) m\u1eabu \u0111\u00e3 chu\u1ea9n b\u1ecb v\u00e0 chuy\u1ec3n v\u00e0o c\u1ed1c 20ml.
\n3. \u0110o dung d\u1ecbch tinh b\u1ed9t 5ml, dung d\u1ecbch m\u1eabu 10ml (th\u1eed nghi\u1ec7m m\u1eabu 2) v\u00e0 dung d\u1ecbch i\u1ed1t 10ml, \u0111\u1eb7t trong b\u1ec3 n\u01b0\u1edbc 40 \u2103 ri\u00eang trong 15 ph\u00fat.
\n4. Sau 5 ph\u00fat, chuy\u1ec3n dung d\u1ecbch h\u1ed7n h\u1ee3p 1.0ml th\u00e0nh dung d\u1ecbch i\u1ed1t n\u00f3ng 10ml v\u00e0 pha lo\u00e3ng v\u1edbi l\u01b0\u1ee3ng n\u01b0\u1edbc \u0111\u01b0\u1ee3c ghi l\u1ea1i (\u0111\u01b0\u1ee3c ghi l\u1ea1i trong th\u1eed nghi\u1ec7m ti\u00eau chu\u1ea9n c\u1ee7a b\u01b0\u1edbc dung d\u1ecbch tinh b\u1ed9t) v\u00e0 tr\u1ed9n l\u1ea1i.
\n5. N\u1ebfu \u0111\u1ed9 h\u1ea5p th\u1ee5 l\u1edbn h\u01a1n 0,235, b\u01b0\u1edbc th\u1ee9 4 c\u1ea7n \u0111\u01b0\u1ee3c l\u1eb7p l\u1ea1i cho \u0111\u1ebfn khi \u0111\u1ed9 h\u1ea5p th\u1ee5 nh\u1ecf h\u01a1n 0,235.
\n6. Trong qu\u00e1 tr\u00ecnh \u0111o, dung d\u1ecbch h\u1ed7n h\u1ee3p, dung d\u1ecbch i\u1ed1t v\u00e0 n\u01b0\u1edbc c\u1ea7n \u0111\u01b0\u1ee3c duy tr\u00ec \u1edf 40 \u2103 n\u01b0\u1edbc.<\/p>\n

T\u00ednh to\u00e1n
\nTr\u00ean gi\u1ea5y t\u1ecda \u0111\u1ed9 logarit, \u0111\u1eb7t t\u00ean tr\u1ee5c y l\u00e0 \u0111\u1ed9 h\u1ea5p th\u1ee5 (%) v\u00e0 tr\u1ee5c x theo th\u1eddi gian (ph\u00fat).<\/p>\n

\"\"<\/p>\n

Trong \u0111\u00f3 x l\u00e0 s\u1ed1 diastase trong dung d\u1ecbch (ml\/(g \u00b7 h)), t l\u00e0 th\u1eddi gian t\u01b0\u01a1ng \u1ee9ng (min).<\/p>\n

K\u1ebft qu\u1ea3
\nB\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng ph\u01b0\u01a1ng ph\u00e1p n\u00e0y, \u0111\u1ed9 l\u1eb7p l\u1ea1i v\u00e0 \u0111\u1ed9 t\u00e1i l\u1eadp c\u1ee7a th\u00ed nghi\u1ec7m l\u00e0 \u226595%.<\/p>","protected":false},"excerpt":{"rendered":"

Principle After putting starch solution into honey sample solution, some starch is hydrolysis by diastase in honey. The starch residue appears as bluish violet color after mixing with iodine. As the reaction process, the color disappears finally. By using UV-Vis Spectrophotometer at 660nm wavelength, the time required to reach the specific absorbance can be measured. […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[73],"tags":[],"class_list":["post-2799","post","type-post","status-publish","format-standard","hentry","category-food-and-drugs"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts\/2799","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/comments?post=2799"}],"version-history":[{"count":0,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/posts\/2799\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/media?parent=2799"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/categories?post=2799"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.pgeneral.com\/vi\/wp-json\/wp\/v2\/tags?post=2799"}],"curies":[{"name":"WP","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}