{"id":4001,"date":"2025-10-10T17:02:37","date_gmt":"2025-10-10T09:02:37","guid":{"rendered":"https:\/\/www.pgeneral.com\/?p=4001"},"modified":"2025-10-10T17:02:37","modified_gmt":"2025-10-10T09:02:37","slug":"determination-of-iron-in-zinc-alloy-flame-atomic-absorption-spectrometry","status":"publish","type":"post","link":"https:\/\/www.pgeneral.com\/tr\/applications\/determination-of-iron-in-zinc-alloy-flame-atomic-absorption-spectrometry\/","title":{"rendered":"\u00c7inko Ala\u015f\u0131m\u0131nda Demirin Belirlenmesi (Alev Atom Emme Spektrometrisi)"},"content":{"rendered":"

1. Method Overview<\/h2>\n
The sample is digested with hydrochloric acid, hydrogen peroxide, and nitric acid solutions. In a dilute hydrochloric acid medium, the absorbance of iron is measured using an air-acetylene flame at a wavelength of 248.3 nm with an atomic absorption spectrometer.<\/div>\n
\n

2. Instruments and Reagents<\/h2>\n

2.1 Instruments and Equipment<\/h3>\n

2.1.1 Testing Instruments<\/h4>\n
\n
\n\n\n\n\n\n\n\n
Serial No.<\/th>\nName<\/th>\nQuantity<\/th>\nTechnical Requirements<\/th>\nAccessories<\/th>\n<\/tr>\n<\/thead>\n
1<\/td>\nFlame Atomic Absorption Spectrophotometer<\/td>\n1 set<\/td>\n–<\/td>\nIron Hollow Cathode Lamp<\/td>\n<\/tr>\n
2<\/td>\nAir Compressor<\/td>\n1 set<\/td>\nRated discharge pressure: 0.3 MPa<\/td>\n–<\/td>\n<\/tr>\n
3<\/td>\nAcetylene Gas<\/td>\n1 cylinder<\/td>\nPurity \u2265 99.99%<\/td>\n–<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n

2.1.2 Sample Pretreatment Equipment<\/h4>\n
\n
\n\n\n\n\n\n\n\n\n\n\n\n
Serial No.<\/th>\nName<\/th>\nQuantity<\/th>\nTechnical Requirements<\/th>\nAccessories<\/th>\n<\/tr>\n<\/thead>\n
1<\/td>\nElectronic Balance<\/td>\n1 set<\/td>\nSensitivity: 0.1 mg<\/td>\n–<\/td>\n<\/tr>\n
2<\/td>\nBeaker<\/td>\nSeveral<\/td>\nVolume: 100 mL<\/td>\n–<\/td>\n<\/tr>\n
3<\/td>\nGlass Watch Glass<\/td>\nSeveral<\/td>\nDiameter: 70 mm<\/td>\n–<\/td>\n<\/tr>\n
4<\/td>\nAdjustable Hot Plate<\/td>\n1 set<\/td>\nRated temperature range: Room temperature ~ 300 \u2103<\/td>\n–<\/td>\n<\/tr>\n
5<\/td>\nMicropipette<\/td>\n1 each<\/td>\nRanges: 20 \u03bcL ~ 200 \u03bcL, 100 \u03bcL ~ 1000 \u03bcL, 1000 \u03bcL ~ 5000 \u03bcL<\/td>\n–<\/td>\n<\/tr>\n
6<\/td>\nVolumetric Flask<\/td>\nSeveral<\/td>\nVolume: 100 mL<\/td>\n–<\/td>\n<\/tr>\n
7<\/td>\nColorimetric Tube<\/td>\nSeveral<\/td>\nVolumes: 25 mL, 50 mL<\/td>\n–<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n

2.2 Reagents<\/h3>\n

2.2.1 Raw Reagents<\/h4>\n
\n
\n\n\n\n\n\n\n\n
Serial No.<\/th>\nName<\/th>\nTechnical Requirements<\/th>\nRemarks<\/th>\n<\/tr>\n<\/thead>\n
1<\/td>\nNitric Acid<\/td>\nMOS Grade<\/td>\n–<\/td>\n<\/tr>\n
2<\/td>\nHydrochloric Acid<\/td>\nGuaranteed Reagent (GR)<\/td>\n–<\/td>\n<\/tr>\n
3<\/td>\nHydrogen Peroxide<\/td>\nGuaranteed Reagent (GR)<\/td>\n–<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n

2.2.2 Prepared Reagents<\/h4>\n
\n
\n\n\n\n\n\n\n
Serial No.<\/th>\nName<\/th>\nPreparation Method<\/th>\nRemarks<\/th>\n<\/tr>\n<\/thead>\n
1<\/td>\nHydrochloric Acid Solution (1+5)<\/td>\nMeasure 20 mL of nitric acid and mix it uniformly with 100 mL of water.<\/td>\nNote: There may be a typo in the original document (nitric acid mentioned for hydrochloric acid solution preparation); the preparation method follows the original text.<\/em><\/td>\n<\/tr>\n
2<\/td>\nHydrochloric Acid Solution (1+1)<\/td>\nMeasure 50 mL of hydrochloric acid and mix it uniformly with 50 mL of water.<\/td>\n–<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n

2.3 Reference Standards<\/h3>\n

2.3.1 Stock Solution<\/h4>\n
Note: The original document lacks specific information (serial number, No., name, technical requirements, remarks) for the stock solution. Please refer to the actual standard substance used in the experiment (typically an iron single-element standard solution with a concentration of 1000 \u03bcg\/mL, provided by a qualified institution such as the National Institute of Metrology, China).<\/em><\/div>\n

3. Operational Procedures<\/h2>\n

3.1 Sample Preparation<\/h3>\n

3.1.1 Preparation of Test Solution<\/h4>\n
    \n
  1. \n
    Sample Pretreatment<\/p>\n
    <\/div>\n

    Weigh 0.5 g of the sample (accurate to 0.0001 g) and place it in a 100 mL beaker. Add 5 mL of hydrochloric acid solution (1+1), add 1 mL of hydrogen peroxide dropwise, cover with a watch glass, and heat on a hot plate at 150 \u2103 until the reaction is complete. Remove and cool, then add 0.5 mL of nitric acid, heat again at 180 \u2103 for 30 minutes. Remove and cool, transfer the solution to a 50 mL colorimetric tube with deionized water, dilute to the marked volume with deionized water, and mix well. Perform a blank test following the same procedure as the sample.<\/p><\/div>\n<\/li>\n

  2. \n
    Preparation of Sample Test Solution<\/p>\n
    <\/div>\n

    Accurately pipette 5 mL of the original sample solution into a 25 mL colorimetric tube, add 2.5 mL of hydrochloric acid solution (1+5), dilute to the marked volume with deionized water, and mix well. Prepare a blank test solution simultaneously; except for not adding the sample, the type and amount of reagents added to the blank test solution are the same as those of the test solution.<\/p><\/div>\n<\/li>\n<\/ol>\n

    3.1.2 Preparation of Standard Solutions<\/h4>\n
    Preparation of Iron Standard Series<\/p>\n
    <\/div>\n

    Pipette 0 \u03bcL, 25 \u03bcL, 50 \u03bcL, 100 \u03bcL, 150 \u03bcL, and 200 \u03bcL of the iron standard solution (1000 \u03bcg\/mL) into separate 100 mL volumetric flasks respectively. Add 10 mL of hydrochloric acid solution (1+5) to each flask, dilute to the marked volume with deionized water, and mix well. The concentrations of this standard series are 0 \u03bcg\/mL, 0.25 \u03bcg\/mL, 0.50 \u03bcg\/mL, 1.00 \u03bcg\/mL, 1.50 \u03bcg\/mL, and 2.00 \u03bcg\/mL.<\/p><\/div>\n

    3.2 Sample Testing<\/h3>\n

    1) Testing Conditions<\/h4>\n
    Reference Conditions for Flame Atomic Absorption Spectrophotometer<\/strong><\/div>\n
    <\/div>\n
    \n
    \n\n\n\n\n\n\n\n\n\n\n
    Parameter<\/th>\nSpecification<\/th>\n<\/tr>\n<\/thead>\n
    Wavelength<\/td>\n248.3 nm<\/td>\n<\/tr>\n
    Spectral Bandwidth<\/td>\n0.2 nm<\/td>\n<\/tr>\n
    Element Lamp Current<\/td>\n4.0 mA<\/td>\n<\/tr>\n
    Burner Height<\/td>\n8 mm<\/td>\n<\/tr>\n
    Fuel Flow Rate<\/td>\n1700 mL\/min<\/td>\n<\/tr>\n
    Air Compressor Pressure<\/td>\n0.22 MPa<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n

    2) Sample Testing<\/h4>\n
    Introduce the iron standard series solutions into the flame atomizer in ascending order of concentration, and measure their absorbance. Plot a standard curve with the mass concentration of iron in the standard series as the abscissa and the corresponding absorbance as the ordinate.<\/div>\n
    <\/div>\n
    Under the same experimental conditions as those for determining the standard solutions, introduce the blank solution and the sample test solution into the atomizer respectively, measure their corresponding absorbance, and perform quantitative determination by comparison with the standard series.<\/div>\n<\/div>\n