{"id":3997,"date":"2025-10-10T16:54:03","date_gmt":"2025-10-10T08:54:03","guid":{"rendered":"https:\/\/www.pgeneral.com\/?p=3997"},"modified":"2025-10-10T16:54:03","modified_gmt":"2025-10-10T08:54:03","slug":"determination-of-calcium-in-milk-powder-flame-atomic-absorption-spectrometry","status":"publish","type":"post","link":"https:\/\/www.pgeneral.com\/tr\/applications\/determination-of-calcium-in-milk-powder-flame-atomic-absorption-spectrometry","title":{"rendered":"S\u00fct Tozu'ndaki Kalsiyumun Belirlenmesi (Alev Atom Emme Spektrometrisi)"},"content":{"rendered":"

1. Y\u00f6ntem Genel Bak\u0131\u015f<\/h2>\n
\u00d6rnek sindirildikten sonra, serbest b\u0131rak\u0131c\u0131 madde olarak bir lantan \u00e7\u00f6z\u00fcm\u00fc eklenir. \u00d6rnek daha sonra atomik emim spektrometrinde bir alev taraf\u0131ndan atomla\u015ft\u0131r\u0131l\u0131r ve emici 422,7 nm dalga boyunda \u00f6l\u00e7\u00fcl\u00fcr. Belirli bir konsantrasyon aral\u0131\u011f\u0131nda, emici kalsiyum i\u00e7eri\u011fine orant\u0131l\u0131d\u0131r. Niyetsel belirleme standart bir seriyle kar\u015f\u0131la\u015ft\u0131r\u0131larak ger\u00e7ekle\u015ftirilir.<\/div>\n
\n

2. Aletler ve Reagentler<\/h2>\n

2.1 Aletler ve Ekipmanlar<\/h3>\n

2.1.1 Test Aletleri<\/h4>\n
\n
\n\n\n\n\n\n\n\n
Seri numaras\u0131.<\/th>\n\u0130sim<\/th>\nMiktar<\/th>\nTeknik Gereksinimler<\/th>\nAksesuarlar<\/th>\n<\/tr>\n<\/thead>\n
1<\/td>\nAlev Atom Emme Spektrofotometri<\/td>\n1 set<\/td>\n–<\/td>\nKalsiyum Bo\u015f Katod Lambas\u0131<\/td>\n<\/tr>\n
2<\/td>\nHava Kompres\u00f6r\u00fc<\/td>\n1 set<\/td>\nNominal bo\u015faltma bas\u0131nc\u0131: 0.3 MPa<\/td>\n–<\/td>\n<\/tr>\n
3<\/td>\nAsetilen Gaz\u0131<\/td>\n1 silindir<\/td>\nSafl\u0131k \u2265% 99,99<\/td>\n–<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n

2.1.2 \u00d6rnek \u00d6n Tedavi Ekipmanlar\u0131<\/h4>\n
\n
\n\n\n\n\n\n\n\n\n\n\n\n\n
Seri numaras\u0131.<\/th>\n\u0130sim<\/th>\nMiktar<\/th>\nTeknik Gereksinimler<\/th>\nAksesuarlar<\/th>\n<\/tr>\n<\/thead>\n
1<\/td>\nElektronik Denge<\/td>\n1 set<\/td>\nHassasiyet: 0.1 mg<\/td>\n–<\/td>\n<\/tr>\n
2<\/td>\nKuvars Crucible<\/td>\nBirka\u00e7<\/td>\nHacim: 100 ml<\/td>\n–<\/td>\n<\/tr>\n
3<\/td>\nAyarlanabilir Elektrikli F\u0131r\u0131n<\/td>\n1 set<\/td>\nNominal g\u00fc\u00e7: 2000 W<\/td>\n–<\/td>\n<\/tr>\n
4<\/td>\nMuffle F\u0131r\u0131n\u0131<\/td>\n1 set<\/td>\nNominal s\u0131cakl\u0131k: 1000 \u2103<\/td>\n–<\/td>\n<\/tr>\n
5<\/td>\nAyarlanabilir S\u0131cak Plaka<\/td>\n1 set<\/td>\nNominal s\u0131cakl\u0131k aral\u0131\u011f\u0131: Oda s\u0131cakl\u0131\u011f\u0131 ~ 300 \u2103<\/td>\n–<\/td>\n<\/tr>\n
6<\/td>\nMikropipet<\/td>\nHer biri 1<\/td>\nAral\u0131klar\u0131: 100 \u03bcL ~ 1000 \u03bcL, 1000 \u03bcL ~ 5000 \u03bcL<\/td>\n–<\/td>\n<\/tr>\n
7<\/td>\nhacimli \u015fi\u015fe<\/td>\nBirka\u00e7<\/td>\nHacimler: 100 mL, 1000 mL<\/td>\n–<\/td>\n<\/tr>\n
8<\/td>\nKolorimetrik T\u00fcp<\/td>\nBirka\u00e7<\/td>\nHacim: 50 ml<\/td>\n–<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n

2.2 Reagentler<\/h3>\n

2.2.1 Ham Reagentler<\/h4>\n
\n
\n\n\n\n\n\n\n\n
Seri numaras\u0131.<\/th>\n\u0130sim<\/th>\nTeknik Gereksinimler<\/th>\nNotlar<\/th>\n<\/tr>\n<\/thead>\n
1<\/td>\nNitrik Asit<\/td>\nMOS S\u0131n\u0131f\u0131<\/td>\n–<\/td>\n<\/tr>\n
2<\/td>\nHidroklorik Asit<\/td>\nGarantili Reagent (GR)<\/td>\n–<\/td>\n<\/tr>\n
3<\/td>\nLantan Oksit<\/td>\nGarantili Reagent (GR)<\/td>\n–<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n

2.2 Haz\u0131rlanan Reagentler<\/h4>\n
\n
\n\n\n\n\n\n\n\n
Seri numaras\u0131.<\/th>\n\u0130sim<\/th>\nHaz\u0131rlama Y\u00f6ntemi<\/th>\nNotlar<\/th>\n<\/tr>\n<\/thead>\n
1<\/td>\nNitrik Asit \u00c7\u00f6z\u00fcm\u00fc (5 95)<\/td>\n50 ml nitrik asit \u00f6l\u00e7\u00fcn ve 950 ml su ile ayn\u0131 \u015fekilde kar\u0131\u015ft\u0131r\u0131n.<\/td>\n–<\/td>\n<\/tr>\n
2<\/td>\nNitrik Asit \u00c7\u00f6z\u00fcm\u00fc (1 1)<\/td>\n50 ml nitrik asit \u00f6l\u00e7\u00fcn ve 50 ml su ile ayn\u0131 \u015fekilde kar\u0131\u015ft\u0131r\u0131n.<\/td>\n–<\/td>\n<\/tr>\n
3<\/td>\nHidroklorik Asit \u00c7\u00f6z\u00fcm\u00fc (1 1)<\/td>\n50 mL hidroklorik asit \u00f6l\u00e7\u00fcn ve 50 mL suyla ayn\u0131 \u015fekilde kar\u0131\u015ft\u0131r\u0131n.<\/td>\n–<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n

2.3 Referans Standartlar\u0131<\/h3>\n

2.3.1 Stok \u00c7\u00f6z\u00fcm\u00fc<\/h4>\n
\n
\n\n\n\n\n\n
Seri numaras\u0131.<\/th>\n- Hay\u0131r. - Hay\u0131r.<\/th>\n\u0130sim<\/th>\nTeknik Gereksinimler<\/th>\nNotlar<\/th>\n<\/tr>\n<\/thead>\n
1<\/td>\nGBW (E) 080261<\/td>\nKalsiyum Tek Elemanl\u0131 Standart Madde \u00c7\u00f6z\u00fcm\u00fc<\/td>\nKonsantrasyon: 1000 \u03bcg \/ mL<\/td>\nUlusal Metroloji Enstit\u00fcs\u00fc, \u00c7in<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n

3. Operasyonal Prosed\u00fcrler<\/h2>\n

3.1 \u00d6rnek Haz\u0131rlama<\/h3>\n

3.1.1 Test \u00c7\u00f6z\u00fcm\u00fcn\u00fcn Haz\u0131rlanmas\u0131<\/h4>\n
    \n
  1. \n
    \u00d6rnek \u00d6n muamele<\/p>\n
    <\/div>\n

    \u00d6rne\u011fin 2 g'ini (0,0001 g'e kadar do\u011fru) 100 mL kuvars ti\u00e7e\u011fine do\u011fru bir \u015fekilde a\u011f\u0131rlay\u0131n. \u00c7ama\u011f\u0131 ayarlanabilir bir elektrikli f\u0131r\u0131na yerle\u015ftirin, duman olu\u015fana kadar karbonize etmek i\u00e7in hafif\u00e7e \u0131s\u0131t\u0131n. \u00c7ama\u011f\u0131 \u00e7\u0131kar\u0131n ve bir muffle f\u0131r\u0131n\u0131na aktar\u0131n, sonra 550 \u2103 da 3 ~ 4 saat boyunca k\u00fcl. So\u011futuktan sonra \u00e7akmay\u0131 \u00e7\u0131kar\u0131n. \u00d6rnek tamamen k\u00fcl de\u011filse, birka\u00e7 damla nitrik asit ekleyin, ayarlanabilir bir s\u0131cak plakaya yerle\u015ftirin, dikkatlice kurulu\u011fa buharla\u015fmak i\u00e7in 150 \u2103 da \u0131s\u0131t\u0131n ve sonra \u00f6rnek beyaz k\u00fcle d\u00f6nene kadar 1 ~ 2 saat daha fazla k\u00fcl yapmak i\u00e7in 550 \u2103 da muffle f\u0131r\u0131n\u0131na geri aktar\u0131n. So\u011futuk ve ti\u00e7e\u011fi \u00e7\u0131kar\u0131n, 2 mL nitrik asit \u00e7\u00f6z\u00fcm\u00fc (1 1) ekleyin, ayarlanabilir s\u0131cak plakaya yerle\u015ftirin, \u00f6rne\u011fi \u00e7\u00f6zmek i\u00e7in 150 \u2103de \u0131s\u0131t\u0131n. So\u011futuktan sonra, \u00e7\u00f6z\u00fcm\u00fc 50 mL kolorimetrik t\u00fcpte su ile i\u015faretlenmi\u015f hacime seyreltin. Ayn\u0131 anda bir reaktif bo\u015f testi yap\u0131n.<\/p><\/div>\n<\/li>\n

  2. \n
    \u00d6rnek Test \u00c7\u00f6z\u00fcm\u00fcn\u00fcn Haz\u0131rlanmas\u0131<\/p>\n
    <\/div>\n

    1 mL sindirilmi\u015f \u00e7\u00f6z\u00fcm\u00fc 100 mL hacimli bir kolba pipet edin, 5 mL lantan \u00e7\u00f6z\u00fcm\u00fc (20 g \/ L) ekleyin, i\u015faretlenmi\u015f hacime nitrik asit \u00e7\u00f6z\u00fcm\u00fc (5 95) ile seyreltin ve iyi kar\u0131\u015ft\u0131r\u0131n. Ayn\u0131 \u015fekilde bir reaktif bo\u015flu\u011fu haz\u0131rlay\u0131n. \u00d6rnek eklenmemesi hari\u00e7, bo\u015f test \u00e7\u00f6z\u00fcm\u00fcne eklenen reagentlerin t\u00fcr\u00fc ve miktar\u0131 test \u00e7\u00f6z\u00fcm\u00fcn\u00fcn t\u00fcr\u00fc ve miktar\u0131yla ayn\u0131d\u0131r.<\/p><\/div>\n<\/li>\n<\/ol>\n

    3.1.2 Standart \u00c7\u00f6z\u00fcmlerin Haz\u0131rlanmas\u0131<\/h4>\n
    Kalsiyum Standart Serisinin Haz\u0131rlanmas\u0131<\/p>\n
    <\/div>\n

    0 mL, 0,10 mL, 0,20 mL, 0,30 mL ve 0,40 mL kalsiyum standart \u00e7\u00f6z\u00fcm\u00fcn\u00fc (1000 \u03bcg\/mL) s\u0131ras\u0131yla ayr\u0131 100 mL hacimli \u015fi\u015felere pipet edin. Her kolba 5 mL lantan \u00e7\u00f6z\u00fcm\u00fc (20 g\/L) ekleyin, i\u015faretlenmi\u015f hacme nitrik asit \u00e7\u00f6z\u00fcm\u00fc (5 95) ile seyreltin ve iyi kar\u0131\u015ft\u0131r\u0131n. Bu kalsiyum standart serisinin konsantrasyonlar\u0131 0 \u03bcg\/mL, 1.00 \u03bcg\/mL, 2.00 \u03bcg\/mL, 3.00 \u03bcg\/mL ve 4.00 \u03bcg\/mL'dir.<\/p><\/div>\n

    3.2 \u00d6rnek Testi<\/h3>\n

    1) Test Ko\u015fullar\u0131<\/h4>\n
    Alev Atomik Emme Spektrofotometri i\u00e7in Referans Ko\u015fullar\u0131<\/strong><\/div>\n
    <\/div>\n
    \n
    \n\n\n\n\n\n\n\n\n\n\n
    Parametre<\/th>\n\u00d6zellikler<\/th>\n<\/tr>\n<\/thead>\n
    Dalga Uzunlu\u011fu<\/td>\n422,7 nm'lik<\/td>\n<\/tr>\n
    Spektral Bant Geni\u015fli\u011fi<\/td>\n0,4 nm'lik<\/td>\n<\/tr>\n
    Element Lamba Ak\u0131m\u0131<\/td>\n3.0 mA'l\u0131k<\/td>\n<\/tr>\n
    Yan\u0131c\u0131 Y\u00fcksekli\u011fi<\/td>\n10 mm'lik<\/td>\n<\/tr>\n
    Yak\u0131t Ak\u0131\u015f H\u0131z\u0131<\/td>\n1600 ml \/ dakika<\/td>\n<\/tr>\n
    Hava Kompres\u00f6r\u00fc Bas\u0131nc\u0131<\/td>\n0,22 MPa'n\u0131n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n

    2) \u00d6rnek Test<\/h4>\n
    Kalsiyum standart serisi \u00e7\u00f6z\u00fcmlerini alev atomizat\u00f6r\u00fcne y\u00fckselen konsantrasyon s\u0131ras\u0131nda girin ve emirlerini \u00f6l\u00e7\u00fcn. Standart serideki kalsiyum k\u00fctle konsantrasyonu absisa olarak ve ilgili emici ordinat olarak standart bir e\u011fri \u00e7izin.<\/div>\n
    <\/div>\n
    Standart \u00e7\u00f6z\u00fcmleri belirlemek i\u00e7in yap\u0131lanlarla ayn\u0131 deneysel ko\u015fullar alt\u0131nda, s\u0131ras\u0131yla bo\u015f \u00e7\u00f6z\u00fcm ve \u00f6rnek test \u00e7\u00f6z\u00fcm\u00fcn\u00fc atomizat\u00f6re girin, ilgili emicilerini \u00f6l\u00e7\u00fcn ve standart serilerle kar\u015f\u0131la\u015ft\u0131r\u0131ld\u0131\u011f\u0131nda miktarsal belirleme ger\u00e7ekle\u015ftirin.<\/div>\n<\/div>","protected":false},"excerpt":{"rendered":"

    1. Method Overview After the sample is digested, a lanthanum solution is added as a releasing agent. The sample is then atomized by a flame in an atomic absorption spectrometer, and the absorbance is measured at a wavelength of 422.7 nm. Within a certain concentration range, the absorbance is proportional to the calcium content. Quantitative […]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[36,73],"tags":[],"class_list":["post-3997","post","type-post","status-publish","format-standard","hentry","category-applications","category-food-and-drugs"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/posts\/3997","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/comments?post=3997"}],"version-history":[{"count":0,"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/posts\/3997\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/media?parent=3997"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/categories?post=3997"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/tags?post=3997"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}