{"id":3995,"date":"2025-10-10T16:51:03","date_gmt":"2025-10-10T08:51:03","guid":{"rendered":"https:\/\/www.pgeneral.com\/?p=3995"},"modified":"2025-10-10T16:51:03","modified_gmt":"2025-10-10T08:51:03","slug":"determination-of-lead-in-olive-oil-graphite-furnace-atomic-absorption-spectrometry-2","status":"publish","type":"post","link":"https:\/\/www.pgeneral.com\/tr\/applications\/determination-of-lead-in-olive-oil-graphite-furnace-atomic-absorption-spectrometry-2","title":{"rendered":"Zeytinya\u011f\u0131ndaki Kur\u015fun Belirlenmesi (Grafit F\u0131r\u0131n\u0131 Atom Emme Spektrometrisi)"},"content":{"rendered":"

1. Y\u00f6ntem Genel Bak\u0131\u015f<\/h2>\n
\u00d6rnek mikrodalga ile sindirildikten sonra, atomik emim spektrofotometrinin grafit f\u0131r\u0131n\u0131na enjekte edilir. Elektrotermal atomizasyondan sonra, rezonans hatt\u0131n\u0131 283.3 nm'de emir. Belirli bir konsantrasyon aral\u0131\u011f\u0131nda, emici, kur\u015fun i\u00e7eri\u011fine orant\u0131l\u0131d\u0131r. Niyetsel belirleme standart bir seriyle kar\u015f\u0131la\u015ft\u0131r\u0131larak ger\u00e7ekle\u015ftirilir.<\/div>\n
\n

2. Aletler ve Reagentler<\/h2>\n

2.1 Aletler ve Ekipmanlar<\/h3>\n

2.1.1 Test Aletleri<\/h4>\n
\n
\n\n\n\n\n\n\n\n
Seri numaras\u0131.<\/th>\n\u0130sim<\/th>\nMiktar<\/th>\nTeknik Gereksinimler<\/th>\nAksesuarlar<\/th>\n<\/tr>\n<\/thead>\n
1<\/td>\nGrafit F\u0131r\u0131n Atom Emme Spektrofotometri<\/td>\n1 set<\/td>\n–<\/td>\nKur\u015fun Bo\u015f Katod Lambas\u0131<\/td>\n<\/tr>\n
2<\/td>\nArgon Gaz\u0131<\/td>\n1 silindir<\/td>\nSafl\u0131k \u2265% 99,99<\/td>\n–<\/td>\n<\/tr>\n
3<\/td>\nSirk\u00fclasyon So\u011futma Su Sistemi<\/td>\n1 set<\/td>\n–<\/td>\n–<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n

2.1.2 \u00d6rnek \u00d6n Tedavi Ekipmanlar\u0131<\/h4>\n
\n
\n\n\n\n\n\n\n\n\n\n\n
Seri numaras\u0131.<\/th>\n\u0130sim<\/th>\nMiktar<\/th>\nTeknik Gereksinimler<\/th>\nAksesuarlar<\/th>\n<\/tr>\n<\/thead>\n
1<\/td>\nMikrodalga Sindirim Sistemi<\/td>\n1 set<\/td>\nPlatin direnci ve k\u0131z\u0131l\u00f6tesi s\u0131cakl\u0131k \u00f6l\u00e7\u00fcm\u00fc ile donat\u0131lm\u0131\u015ft\u0131r; bas\u0131n\u00e7 \u00f6l\u00e7\u00fcm do\u011frulu\u011fu: \u00b1 0.1 \u2103<\/td>\n–<\/td>\n<\/tr>\n
2<\/td>\nS\u0131cakl\u0131k Kontrollu S\u0131cak Plaka<\/td>\n1 set<\/td>\n–<\/td>\n–<\/td>\n<\/tr>\n
3<\/td>\nElektronik Denge<\/td>\n1 set<\/td>\nHassasiyet: 0.1 mg<\/td>\n–<\/td>\n<\/tr>\n
4<\/td>\nUltrasaf Su Sistemi<\/td>\n1 set<\/td>\n–<\/td>\n–<\/td>\n<\/tr>\n
5<\/td>\nhacimli \u015fi\u015fe<\/td>\nBirka\u00e7<\/td>\nHacim: 100 ml<\/td>\n–<\/td>\n<\/tr>\n
6<\/td>\nTest T\u00fcp\u00fc<\/td>\nBirka\u00e7<\/td>\nHacim: 10 ml<\/td>\n–<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n

2.2 Reagentler<\/h3>\n

2.2.1 Ham Reagentler<\/h4>\n
\n
\n\n\n\n\n\n\n\n\n
Seri numaras\u0131.<\/th>\n\u0130sim<\/th>\nTeknik Gereksinimler<\/th>\nNotlar<\/th>\n<\/tr>\n<\/thead>\n
1<\/td>\nDiammoniyum Hidrojen Fosfat<\/td>\nAnalitik Reagent (AR)<\/td>\n–<\/td>\n<\/tr>\n
2<\/td>\nHidroklorik Asit<\/td>\nGarantili Reagent (GR)<\/td>\n–<\/td>\n<\/tr>\n
3<\/td>\nNitrik Asit<\/td>\nMOS S\u0131n\u0131f\u0131<\/td>\n–<\/td>\n<\/tr>\n
4<\/td>\nPaladyum Nitrat<\/td>\nAnalitik Reagent (AR)<\/td>\n–<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n

2.2 Haz\u0131rlanan Reagentler<\/h4>\n
\n
\n\n\n\n\n\n\n\n
Seri numaras\u0131.<\/th>\n\u0130sim<\/th>\nHaz\u0131rlama Y\u00f6ntemi<\/th>\nNotlar<\/th>\n<\/tr>\n<\/thead>\n
1<\/td>\n(1 99) Nitrik Asit \u00c7\u00f6z\u00fcm\u00fc<\/td>\n10 mL nitrik asit \u00f6l\u00e7\u00fcn, yava\u015f yava\u015f 990 mL suya ekleyin ve iyi kar\u0131\u015ft\u0131r\u0131n.<\/td>\n–<\/td>\n<\/tr>\n
2<\/td>\n(5 95) Nitrik Asit \u00c7\u00f6z\u00fcm\u00fc<\/td>\n5 mL nitrik asit \u00f6l\u00e7\u00fcn, yava\u015f yava\u015f 95 mL suya ekleyin ve iyi kar\u0131\u015ft\u0131r\u0131n.<\/td>\n–<\/td>\n<\/tr>\n
3<\/td>\nDiammoniyum Hidrojen Fosfat – Paladyum Nitrat \u00c7\u00f6z\u00fcm\u00fc<\/td>\n0,02 g paladyum nitrat a\u011f\u0131rl\u0131k, az miktarda (19) nitrik asit \u00e7\u00f6z\u00fcm\u00fc ile \u00e7\u00f6z\u00fcn. Daha sonra 2 g diyamonyum hidrojen fosfat ekleyin ve \u00e7\u00f6z\u00fcnd\u00fckten sonra (5 95) nitrik asit \u00e7\u00f6z\u00fcm\u00fc ile 100 mL'ye seyreltin. \u0130yi kar\u0131\u015ft\u0131r\u0131n.<\/td>\n–<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n

2.3 Referans Standartlar\u0131<\/h3>\n

2.3.1 Stok \u00c7\u00f6z\u00fcm\u00fc<\/h4>\n
\n
\n\n\n\n\n\n
Seri numaras\u0131.<\/th>\n- Hay\u0131r. - Hay\u0131r.<\/th>\n\u0130sim<\/th>\nTeknik Gereksinimler<\/th>\nNotlar<\/th>\n<\/tr>\n<\/thead>\n
1<\/td>\nGBW 08619'\u0131<\/td>\nKur\u015fun Tek Elemanl\u0131 Standart Madde \u00c7\u00f6z\u00fcm\u00fc<\/td>\nKonsantrasyon: 1000 \u03bcg \/ mL<\/td>\nUlusal Metroloji Enstit\u00fcs\u00fc, \u00c7in<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n

3. Operasyonal Prosed\u00fcrler<\/h2>\n

3.1 \u00d6rnek Haz\u0131rlama<\/h3>\n

3.1.1 Test \u00c7\u00f6z\u00fcm\u00fcn\u00fcn Haz\u0131rlanmas\u0131<\/h4>\n
Kat\u0131 \u00f6rne\u011fin 0,2 g'ini (0,001 g'e kadar do\u011fru) mikrodalga sindirim tank\u0131na do\u011fru bir \u015fekilde a\u011f\u0131rlay\u0131n, 5 mL nitrik asit ekleyin ve \u00f6rne\u011fi mikrodalga sindirim i\u015flemi ad\u0131mlar\u0131na g\u00f6re sindirin. So\u011futuktan sonra sindirim tank\u0131n\u0131 \u00e7\u0131kar\u0131n ve asiti, hacim yakla\u015f\u0131k 1 mL olana kadar 140 \u2103 ~ 160 \u2103 s\u0131cak bir plakada buharla\u015ft\u0131r\u0131n. Sindirim tank\u0131 so\u011fulduktan sonra, sindirim \u00e7\u00f6z\u00fcm\u00fcn\u00fc 25 mL hacimli bir kolba deiyonla\u015ft\u0131r\u0131lm\u0131\u015f su ile aktar\u0131n. Sindirim tank\u0131n\u0131 2 ~ 3 kez k\u00fc\u00e7\u00fck miktarda su ile y\u0131kay\u0131n, y\u0131kama \u00e7\u00f6z\u00fcmlerini hacimli kolba birle\u015ftirin ve i\u015faretlenmi\u015f hacime su ile seyredin. Kullan\u0131m i\u00e7in iyi kar\u0131\u015ft\u0131r\u0131n; Bu \u00f6rnek test \u00e7\u00f6z\u00fcm\u00fcd\u00fcr. Ayn\u0131 anda bir reaktif bo\u015f testi yap\u0131n.<\/div>\n
<\/div>\n
Mikrodalga Is\u0131tma Program\u0131<\/strong><\/div>\n
<\/div>\n
\n
\n\n\n\n\n\n\n\n\n\n
Ad\u0131m<\/th>\nS\u0131cakl\u0131k (\u2103)<\/th>\nTutma S\u00fcresi (min)<\/th>\n<\/tr>\n<\/thead>\n
1<\/td>\n100<\/td>\n3<\/td>\n<\/tr>\n
2<\/td>\n120<\/td>\n3<\/td>\n<\/tr>\n
3<\/td>\n140<\/td>\n3<\/td>\n<\/tr>\n
4<\/td>\n160<\/td>\n3<\/td>\n<\/tr>\n
5<\/td>\n180<\/td>\n30<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n

3.1.2 Standart \u00c7\u00f6z\u00fcmlerin Haz\u0131rlanmas\u0131<\/h4>\n
    \n
  1. \n
    Kur\u015fun Standart Ara \u00c7\u00f6z\u00fcm\u00fcn\u00fcn Haz\u0131rlanmas\u0131<\/p>\n
    <\/div>\n

    Kur\u015fun standart ara \u00e7\u00f6z\u00fcm\u00fc (1,0 \u03bcg\/mL): 0,1 mL kur\u015fun standart \u00e7\u00f6z\u00fcm\u00fcn\u00fc (1000 \u03bcg\/mL) 100 mL hacimli bir kolba do\u011fru bir \u015fekilde pipet edin, i\u015faretlenmi\u015f hacime (1 99) nitrik asit \u00e7\u00f6z\u00fcm\u00fc ile seyreltin ve iyi kar\u0131\u015ft\u0131r\u0131n.<\/p><\/div>\n<\/li>\n

  2. \n
    Kur\u015fun Standart Serisinin Haz\u0131rlanmas\u0131<\/p>\n
    <\/div>\n

    0,0, 0,1, 0,2, 0,3 ve 0,4 mL kur\u015fun standart ara \u00e7\u00f6z\u00fcm\u00fcn\u00fc (1,0 \u03bcg\/mL) s\u0131ras\u0131yla ayr\u0131 10 mL hacimli \u015fi\u015felere do\u011fru bir \u015fekilde pipetleyin. 0,0, 10,0, 20,0, 30,0 ve 40,0 ng\/mL konsantrasyonlar\u0131nda kur\u015fun standart serisi \u00e7\u00f6z\u00fcmleri elde etmek i\u00e7in (99) nitrik asit \u00e7\u00f6z\u00fcm\u00fc ile i\u015faretlenmi\u015f hacime seyreltin.<\/p><\/div>\n<\/li>\n<\/ol>\n

    3.2 \u00d6rnek Testi<\/h3>\n

    1) Test Ko\u015fullar\u0131<\/h4>\n
    Grafit F\u0131r\u0131n Atom Emme Spektrofotometri i\u00e7in Referans Ko\u015fullar\u0131<\/strong><\/div>\n
    <\/div>\n
    \n
    \n\n\n\n\n\n\n\n\n
    Element<\/th>\nKur\u015fun<\/th>\n<\/tr>\n<\/thead>\n
    Dalga Uzunlu\u011fu (nm)<\/td>\n283.3<\/td>\n<\/tr>\n
    Spektral Bant Geni\u015fli\u011fi (nm)<\/td>\n0.4<\/td>\n<\/tr>\n
    Element Lamba Ak\u0131m\u0131 (mA)<\/td>\n2<\/td>\n<\/tr>\n
    Arka plan d\u00fczeltme y\u00f6ntemi<\/td>\nLamba<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n
    <\/div>\n
    Grafit F\u0131r\u0131n Is\u0131tma Program\u0131<\/strong><\/div>\n
    <\/div>\n
    \n
    \n\n\n\n\n\n\n\n\n
    Ad\u0131m<\/th>\nProsed\u00fcr<\/th>\nS\u0131cakl\u0131k (\u2103)<\/th>\nIs\u0131tma S\u00fcresi (s)<\/th>\nTutma S\u00fcresi (s)<\/th>\n<\/tr>\n<\/thead>\n
    1<\/td>\nKurutma<\/td>\n120<\/td>\n10<\/td>\n10<\/td>\n<\/tr>\n
    2<\/td>\nAshing'in<\/td>\n650<\/td>\n10<\/td>\n15<\/td>\n<\/tr>\n
    3<\/td>\nAtomizasyon<\/td>\n1900<\/td>\n0<\/td>\n2<\/td>\n<\/tr>\n
    4<\/td>\nTemizlik<\/td>\n2000<\/td>\n1<\/td>\n2<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n

    2) \u00d6rnek Test<\/h4>\n
    Her kur\u015fun standart serisi \u00e7\u00f6z\u00fcm\u00fcn\u00fcn 10 \u03bcL ve diammoniyum hidrojen fosfat\u0131n 5 \u03bcL enjekte edin – paladyum nitrat \u00e7\u00f6z\u00fcm\u00fc grafit f\u0131r\u0131n\u0131na ayn\u0131 anda k\u00fctle konsantrasyonunun y\u00fckselen s\u0131ras\u0131nda. Atomizasyondan sonra, emici \u00f6l\u00e7\u00fcn. Absisa olarak k\u00fctle konsantrasyonu ve ordinat olarak emici olan standart bir e\u011fri \u00e7izin. \u00d6rnek a\u011f\u0131rl\u0131\u011f\u0131n\u0131 girin, sonra sonu\u00e7lar\u0131 okuyun ve kaydedin.<\/div>\n<\/div>","protected":false},"excerpt":{"rendered":"

    1. Method Overview After the sample is digested by microwave, it is injected into the graphite furnace of an atomic absorption spectrophotometer. After electrothermal atomization, it absorbs the resonance line at 283.3 nm. Within a certain concentration range, the absorbance is proportional to the lead content. Quantitative determination is performed by comparison with a standard […]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[36,73],"tags":[],"class_list":["post-3995","post","type-post","status-publish","format-standard","hentry","category-applications","category-food-and-drugs"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/posts\/3995","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/comments?post=3995"}],"version-history":[{"count":0,"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/posts\/3995\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/media?parent=3995"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/categories?post=3995"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/tags?post=3995"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}