{"id":3888,"date":"2025-09-04T10:08:05","date_gmt":"2025-09-04T02:08:05","guid":{"rendered":"https:\/\/www.pgeneral.com\/?p=3888"},"modified":"2025-09-04T10:08:05","modified_gmt":"2025-09-04T02:08:05","slug":"determination-of-potassium-in-sweet-potato-products-flame-atomic-absorption-spectrometry","status":"publish","type":"post","link":"https:\/\/www.pgeneral.com\/tr\/applications\/determination-of-potassium-in-sweet-potato-products-flame-atomic-absorption-spectrometry","title":{"rendered":"Tatl\u0131 Patates \u00dcr\u00fcnlerinde Potasyumun Belirlenmesi (Alev Atom Emme Spektrometrisi)"},"content":{"rendered":"

1. Y\u00f6ntem Genel Bak\u0131\u015f<\/h2>\n
\u00d6rnek sindirildikten sonra, atomik emi\u015f spektrometerinin alevi taraf\u0131ndan atomla\u015ft\u0131r\u0131l\u0131r ve emici 766,5 nm dalga boyunda \u00f6l\u00e7\u00fcl\u00fcr. Belirli bir konsantrasyon aral\u0131\u011f\u0131nda, emici de\u011fer potasyum i\u00e7eri\u011fine orant\u0131l\u0131d\u0131r ve miqdarl\u0131k standart bir seriyle kar\u015f\u0131la\u015ft\u0131r\u0131larak ger\u00e7ekle\u015ftirilir.<\/div>\n
\n

2. Aletler ve Reagentler<\/h2>\n

2.1 Aletler ve Ekipmanlar<\/h3>\n

2.1.1 Test Aletleri<\/h4>\n
\n
\n\n\n\n\n\n\n\n
Seri numaras\u0131.<\/th>\n\u0130sim<\/th>\nMiktar<\/th>\nTeknik Gereksinimler<\/th>\nAksesuarlar<\/th>\n<\/tr>\n<\/thead>\n
1<\/td>\nAlev Atom Emme Spektrofotometri<\/td>\n1 set<\/td>\n–<\/td>\nPotasyum Bo\u015f Katod Lambas\u0131<\/td>\n<\/tr>\n
2<\/td>\nHava Kompres\u00f6r\u00fc<\/td>\n1 set<\/td>\nNominal bo\u015faltma bas\u0131nc\u0131: 0.3 MPa<\/td>\n–<\/td>\n<\/tr>\n
3<\/td>\nAsetilen Gaz\u0131<\/td>\n1 silindir<\/td>\nSafl\u0131k \u2265% 99,99<\/td>\n–<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n

2.1.2 \u00d6rnek \u00d6n Tedavi Ekipmanlar\u0131<\/h4>\n
\n
\n\n\n\n\n\n\n\n\n\n\n
Seri numaras\u0131.<\/th>\n\u0130sim<\/th>\nMiktar<\/th>\nTeknik Gereksinimler<\/th>\nAksesuarlar<\/th>\n<\/tr>\n<\/thead>\n
1<\/td>\nMikropipet<\/td>\nHer biri 1<\/td>\nAral\u0131klar\u0131: 100 \u00b5L ~ 1000 \u00b5L, 1000 \u00b5L ~ 5000 \u00b5L, 5 ~ 50 \u00b5L<\/td>\n–<\/td>\n<\/tr>\n
2<\/td>\nKolorimetrik T\u00fcp<\/td>\nBirka\u00e7<\/td>\nHacimler: 10 mL, 25 mL<\/td>\n–<\/td>\n<\/tr>\n
3<\/td>\nAyarlanabilir S\u0131cak Plaka<\/td>\n1 set<\/td>\nNominal s\u0131cakl\u0131k: Oda s\u0131cakl\u0131\u011f\u0131 ~ 300 \u2103<\/td>\n–<\/td>\n<\/tr>\n
4<\/td>\nhacimli \u015fi\u015fe<\/td>\nBirka\u00e7<\/td>\nHacimler: 100 mL, 1000 mL<\/td>\n–<\/td>\n<\/tr>\n
5<\/td>\nAnalitik Denge<\/td>\n1 set<\/td>\nHassasiyet: 0.1 mg<\/td>\n–<\/td>\n<\/tr>\n
6<\/td>\nErlenmeyer \u015fi\u015fesi<\/td>\nBirka\u00e7<\/td>\nHacim: 150 ml<\/td>\n–<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

2.2 Reagentler<\/h3>\n

2.2.1 Ham Reagentler<\/h4>\n
\n
\n\n\n\n\n\n
Seri numaras\u0131.<\/th>\n\u0130sim<\/th>\nTeknik Gereksinimler<\/th>\nNotlar<\/th>\n<\/tr>\n<\/thead>\n
1<\/td>\nNitrik Asit<\/td>\nMOS S\u0131n\u0131f\u0131<\/td>\n–<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n

2.2 Haz\u0131rlanan Reagentler<\/h4>\n
\n
\n\n\n\n\n\n
Seri numaras\u0131.<\/th>\n\u0130sim<\/th>\nHaz\u0131rlama Y\u00f6ntemi<\/th>\nNotlar<\/th>\n<\/tr>\n<\/thead>\n
1<\/td>\nNitrik Asit \u00c7\u00f6z\u00fcm\u00fc (5 95)<\/td>\n50 ml nitrik asit \u00f6l\u00e7\u00fcn ve 950 ml su ile ayn\u0131 \u015fekilde kar\u0131\u015ft\u0131r\u0131n.<\/td>\n–<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n

2.3 Referans Standartlar\u0131<\/h3>\n

2.3.1 Stok \u00c7\u00f6z\u00fcm\u00fc<\/h4>\n
\n
\n\n\n\n\n\n
Seri numaras\u0131.<\/th>\n- Hay\u0131r. - Hay\u0131r.<\/th>\n\u0130sim<\/th>\nTeknik Gereksinimler<\/th>\nNotlar<\/th>\n<\/tr>\n<\/thead>\n
1<\/td>\nGBW (E) 080125<\/td>\nPotasyum Tek Elemanl\u0131 Standart \u00c7\u00f6z\u00fcm<\/td>\nKonsantrasyon: 1000 \u03bcg \/ mL<\/td>\nUlusal Metroloji Enstit\u00fcs\u00fc, \u00c7in<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n

3. Operasyon Prosed\u00fcr\u00fc<\/h2>\n

3.1 \u00d6rnek Haz\u0131rlama<\/h3>\n

3.1.1 Test \u00c7\u00f6z\u00fcm\u00fcn\u00fcn Haz\u0131rlanmas\u0131<\/h4>\n
Numunenin 1 ~ 2 g'ini (hassasiyeti 0,001 g) bir Erlenmeyer kolubuna do\u011fru bir \u015fekilde a\u011f\u0131rlay\u0131n, 10 mL nitrik asit ekleyin ve s\u0131cak bir levha \u00fczerinde sindirin (referans ko\u015fullar\u0131: 80 \u2103 \/ 1 saat, 120 \u2103 \/ 1 saat, 140 \u2103 \/ 2 saat, 160 \u2103 \/ 2 saat, sonra s\u0131cakl\u0131\u011f\u0131 180 \u2103 'ye y\u00fckseltin). \u00c7\u00f6z\u00fcm kahverengi olursa, daha fazla nitrik asit ekleyin ve beyaz dumanlar yay\u0131lana kadar sindirime devam edin. \u00c7\u00f6z\u00fcm renksiz ve \u015feffaf veya hafif\u00e7e sar\u0131 hale geldi\u011finde, so\u011futulun ve su ile 10 mL bir kolorimetrik t\u00fcpte i\u015farete kadar seyreltin. Ayn\u0131 anda bir reaktif bo\u015f haz\u0131rlay\u0131n.<\/div>\n

3.1.2 Standart \u00c7\u00f6z\u00fcmlerin Haz\u0131rlanmas\u0131<\/h4>\n
    \n
  1. \n
    Potasyum Standart Ara \u00c7\u00f6z\u00fcm\u00fcn\u00fcn Haz\u0131rlanmas\u0131 (10 \u03bcg \/ mL):
    1 mL potasyum ulusal standart \u00e7\u00f6z\u00fcm\u00fcn\u00fc 100 mL hacimli bir kolba do\u011fru bir \u015fekilde pipetleyin, i\u015farete nitrik asit \u00e7\u00f6z\u00fcm\u00fcn\u00fc (5 99) ekleyin ve standart ara \u00e7\u00f6z\u00fcm (10 \u03bcg\/mL) elde edilir.<\/div>\n<\/li>\n
  2. \n
    Potasyum Standart Serisinin Haz\u0131rlanmas\u0131:
    0,0, 1,0, 2,0, 3,0 ve 4,0 mL potasyum standart ara \u00e7\u00f6z\u00fcm\u00fcn\u00fc (10 \u03bcg\/mL) s\u0131ras\u0131yla ayr\u0131 100 mL hacimli \u015fi\u015felere do\u011fru bir \u015fekilde pipetleyin. 0,0, 0,1, 0,2, 0,3 ve 0,4 \u03bcg\/mL konsantrasyonlar\u0131 olan potasyum standart serisini elde etmek i\u00e7in i\u015farete nitrik asit \u00e7\u00f6z\u00fcm\u00fc (5 95) ekleyin.<\/div>\n<\/li>\n<\/ol>\n

    3.2 \u00d6rnek Testi<\/h3>\n
      \n
    1. \n
      Test Ko\u015fullar\u0131 Alev atomik emim spektrofotometri i\u00e7in referans test ko\u015fullar\u0131: | Parametre | \u00d6zellikler | | Dalga Boyu | 766.5 nm | | Spektral Bant Geni\u015fli\u011fi | 0.2 nm | | Element Lamba Ak\u0131m\u0131 | 2.0 mA | | Yan\u0131c\u0131 Y\u00fcksekli\u011fi | 5 mm | | Yak\u0131t Gaz\u0131 Ak\u0131\u015f H\u0131z\u0131 | 1500 mL \/ dakika | | Hava Kompres\u00f6r\u00fc Bas\u0131nc\u0131 | 0.22 MPa |<\/div>\n<\/li>\n
    2. \n
      \u00d6rnek Testi Potasyum standart seri \u00e7\u00f6z\u00fcmlerini alev atomizat\u00f6r\u00fcne y\u00fckselen konsantrasyon s\u0131ras\u0131nda tan\u0131t\u0131n ve emici de\u011ferleri \u00f6l\u00e7\u00fcn. Standart seri \u00e7\u00f6z\u00fcmlerindeki potasyum k\u00fctle konsantrasyonu absisa olarak ve ilgili emici de\u011ferleri ordinat olarak standart bir e\u011fri \u00e7izin.<\/div>\n<\/li>\n<\/ol>\n

       <\/p>\n

      Standart \u00e7\u00f6z\u00fcmleri belirlemek i\u00e7in yap\u0131lanlarla ayn\u0131 deneysel ko\u015fullar alt\u0131nda, s\u0131ras\u0131yla bo\u015f \u00e7\u00f6z\u00fcm ve \u00f6rnek test \u00e7\u00f6z\u00fcm\u00fcn\u00fc atomizat\u00f6re girin, kar\u015f\u0131l\u0131k gelen emici de\u011ferlerini \u00f6l\u00e7\u00fcn ve standart serilerle kar\u015f\u0131la\u015ft\u0131r\u0131larak miqdarl\u0131k ger\u00e7ekle\u015ftirin.<\/div>\n

      3.3 Sonu\u00e7 Hesaplamas\u0131<\/h3>\n
      Numunedeki potasyum i\u00e7eri\u011fi a\u015fa\u011f\u0131daki form\u00fcl\u00fc kullanarak hesaplan\u0131r:<\/div>\n

      \"\"<\/p>\n

      Nerede:<\/div>\n
        \n
      • X<\/span>\u00a0= Numunedeki potasyum i\u00e7eri\u011fi, birim: kilogram ba\u015f\u0131na miligram (mg\/kg);<\/li>\n
      • (rho)<\/span>\u00a0= \u00d6rnek test \u00e7\u00f6z\u00fcm\u00fcnde potasyum k\u00fctle konsantrasyonu, birim: mililitre ba\u015f\u0131na mikrogram (\u03bcg\/mL);<\/li>\n
      • (rho_{0})<\/span>\u00a0= Bo\u015f \u00e7\u00f6z\u00fcmdeki potasyum k\u00fctle konsantrasyonu, birim: mililitre ba\u015f\u0131na mikrogram (\u03bcg\/mL);<\/li>\n
      • V'nin<\/span>\u00a0= Sabit hacmdan sonra sindirilen \u00f6rnek \u00e7\u00f6z\u00fcm\u00fcn\u00fcn hacmi, birim: mililitre (mL);<\/li>\n
      • F<\/span>\u00a0= sindirilmi\u015f \u00f6rnek \u00e7\u00f6z\u00fcm\u00fcn\u00fcn seyreltme fakt\u00f6r\u00fc;<\/li>\n
      • M<\/span>\u00a0= \u00d6rne\u011fin k\u00fctlesi, birim: gram (g).<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>","protected":false},"excerpt":{"rendered":"

        1. Method Overview After the sample is digested, it is atomized by the flame of an atomic absorption spectrometer, and the absorbance is measured at a wavelength of 766.5 nm. Within a certain concentration range, the absorbance value is proportional to the potassium content, and quantification is performed by comparison with a standard series. 2. […]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[36,73],"tags":[],"class_list":["post-3888","post","type-post","status-publish","format-standard","hentry","category-applications","category-food-and-drugs"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/posts\/3888","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/comments?post=3888"}],"version-history":[{"count":0,"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/posts\/3888\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/media?parent=3888"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/categories?post=3888"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/tags?post=3888"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}