{"id":3729,"date":"2025-07-15T14:28:11","date_gmt":"2025-07-15T06:28:11","guid":{"rendered":"https:\/\/www.pgeneral.com\/?p=3729"},"modified":"2025-07-15T14:28:11","modified_gmt":"2025-07-15T06:28:11","slug":"determination-of-palladium-in-oseltamivir-phosphate-by-graphite-furnace-atomic-absorption-spectrometry","status":"publish","type":"post","link":"https:\/\/www.pgeneral.com\/tr\/applications\/determination-of-palladium-in-oseltamivir-phosphate-by-graphite-furnace-atomic-absorption-spectrometry","title":{"rendered":"Grafit F\u0131r\u0131n\u0131 Atom Emme Spektrometrisi ile Oseltamivir Fosfat\u0131nda Paldiyumun Belirlenmesi"},"content":{"rendered":"
    \n
  1. Y\u00f6ntem Genel Bak\u0131\u015f<\/li>\n<\/ol>\n

    \u00d6rnek% 2 nitrik asitte \u00e7\u00f6z\u00fcld\u00fckten sonra, atomik emim spektrofotometrinin grafit f\u0131r\u0131n\u0131na enjekte edilir. Elektrotermal atomizasyondan sonra, rezonans hatt\u0131n\u0131 247.6nm'de emir. Belirli bir konsantrasyon aral\u0131\u011f\u0131nda, emici, paladyum i\u00e7eri\u011fine orant\u0131l\u0131d\u0131r ve standart serilerle kar\u015f\u0131la\u015ft\u0131r\u0131larak miqdarl\u0131k yap\u0131l\u0131r.<\/p>\n

      \n
    1. 2. Aletler ve Reagentler<\/li>\n<\/ol>\n

      2.1 Aletler ve Ekipmanlar<\/p>\n

      2.1.1 Tespit Aletleri<\/p>\n

      Grafit f\u0131r\u0131n atomik emim spektrofotometri (Palladium bo\u015f katot lambas\u0131)<\/p>\n

      Argon gaz\u0131 1 silindir Safl\u0131k \u2265% 99,99<\/p>\n

      So\u011futma dola\u015f\u0131m su sistemi (Nominal su pompas\u0131 ak\u0131\u015f\u0131: 3.5L \/ dakika)<\/p>\n

      2.1.2 \u00d6n Tedavi Ekipmanlar\u0131<\/p>\n

      Elektronik denge<\/p>\n

      Ultrasaf su sistemi<\/p>\n

      Mikropipet<\/p>\n

      Hacimli \u015fi\u015fe<\/p>\n

      Test t\u00fcp\u00fc Birka\u00e7<\/p>\n

      2.2 Reagentler<\/p>\n

      2.2.1 Reagentler<\/p>\n

      Nitrik asit<\/p>\n

      2.2 Haz\u0131rlanan Reagentler<\/p>\n

      (2 98) Nitrik asit \u00e7\u00f6z\u00fcm\u00fc 20mL nitrik asit \u00f6l\u00e7\u00fcn, yava\u015f yava\u015f 980mL suya ekleyin ve iyi kar\u0131\u015ft\u0131r\u0131n.<\/p>\n

        \n
      1. 3. Operasyon S\u00fcreci<\/li>\n<\/ol>\n

        3.1 \u00d6rnek Tedavi<\/p>\n

        3.1.1 Test \u00c7\u00f6z\u00fcm\u00fcn\u00fcn Haz\u0131rlanmas\u0131<\/p>\n

        \u00d6rne\u011fin 0,04 g'ini 10mL test t\u00fcb\u00fcne do\u011fru bir \u015fekilde a\u011f\u0131rlay\u0131n, i\u015farete (2 98) nitrik asit \u00e7\u00f6z\u00fcm\u00fc ile seyreltin, iyi kar\u0131\u015ft\u0131r\u0131n ve \u00f6rnek test \u00e7\u00f6z\u00fcm\u00fc olarak bir yana koyun. Ayn\u0131 anda bir reaktif bo\u015f \u00e7\u00f6z\u00fcm\u00fc haz\u0131rlay\u0131n.<\/p>\n

        3.1.2 Standart \u00c7\u00f6z\u00fcmlerin Haz\u0131rlanmas\u0131<\/p>\n

        1) Paladyum standart ara \u00e7\u00f6z\u00fcm\u00fcn\u00fcn haz\u0131rlanmas\u0131:<\/p>\n

        Paladyum standart \u00e7\u00f6z\u00fcm\u00fc (1.0\u03bcg\/mL): 0.1mL paladyum standart \u00e7\u00f6z\u00fcm\u00fcn\u00fc (1000\u03bcg\/mL) 100 mL hacimli bir kolba do\u011fru bir \u015fekilde pipet edin, (2 98) nitrik asit \u00e7\u00f6z\u00fcm\u00fc ile i\u015farete seyreltin ve iyi sallay\u0131n.<\/p>\n

          \n
        • Paladyum standart serisinin haz\u0131rlanmas\u0131:<\/li>\n<\/ul>\n

          0,0, 0,1, 0,2, 0,3 ve 0,4 mL paladyum standart \u00e7\u00f6z\u00fcm\u00fcn\u00fc (1,0 \u03bcg\/mL) s\u0131ras\u0131yla 10mL hacimli \u015fi\u015felere do\u011fru bir \u015fekilde pipetleyin, 0,0, 10,0, 20,0, 30,0, 40,0 ng\/mL konsantrasyonlar\u0131nda paladyum standart serisi \u00e7\u00f6z\u00fcmleri elde etmek i\u00e7in (2 98) nitrik asit \u00e7\u00f6z\u00fcm\u00fcn\u00fc ile i\u015farete seyretin.<\/p>\n

          3.2 \u00d6rnek Testi<\/p>\n

          1) Test Ko\u015fullar\u0131<\/p>\n

          Grafit f\u0131r\u0131n atomik emim spektrofotometri tespiti i\u00e7in referans ko\u015fullar\u0131<\/p>\n

          Eleman\u0131 Palladium<\/p>\n

          Dalga boyu (nm) 247.6<\/p>\n

          Spektral bant geni\u015fli\u011fi (nm) 0.2<\/p>\n

          Element lamba ak\u0131m\u0131 (mA) 6<\/p>\n

          Arka plan d\u00fczeltme y\u00f6ntemi Deuterium lambas\u0131<\/p>\n

          2) \u00d6rnek Test
          \nKitle konsantrasyonunu artt\u0131rmak i\u00e7in 15 \u03bcL paladyum standart serisi \u00e7\u00f6z\u00fcmlerini grafit f\u0131r\u0131n\u0131na enjekte edin. Atomizasyondan sonra, emici \u00f6l\u00e7\u00fcn. Absisa olarak k\u00fctle konsantrasyonu ve ordinat olarak emici olan standart bir e\u011fri \u00e7izin. Verileri okuyun, \u00f6rnek a\u011f\u0131rl\u0131\u011f\u0131n\u0131 girin ve sonu\u00e7lar\u0131 kontrol edin ve kaydedin.<\/p>\n

            \n
          1. Sonu\u00e7 hesaplamas\u0131<\/li>\n<\/ol>\n

            \"\"<\/p>\n

            Nerede:<\/p>\n

            X — Numunedeki paladyum i\u00e7eri\u011fi (mg\/kg);<\/p>\n

            \u03c1– \u00d6rnek test \u00e7\u00f6z\u00fcm\u00fcnde paladyum k\u00fctle konsantrasyonu (\u03bcg \/ L);<\/p>\n

            \u03c10\u00a0— Bo\u015f \u00e7\u00f6z\u00fcmdeki paladyum k\u00fctle konsantrasyonu (\u03bcg \/ L);<\/p>\n

            V — Sabit hacmdan sonra \u00f6rnek sindirim \u00e7\u00f6z\u00fcm\u00fcn\u00fcn hacmi (mL);<\/p>\n

            m — \u00d6rne\u011fin k\u00fctlesi (g);<\/p>\n

            1000 — D\u00f6n\u00fc\u015f\u00fcm fakt\u00f6r\u00fc.<\/p>","protected":false},"excerpt":{"rendered":"

            Method Overview After the sample is dissolved in 2% nitric acid, it is injected into the graphite furnace of an atomic absorption spectrophotometer. After electrothermal atomization, it absorbs the resonance line at 247.6nm. Within a certain concentration range, the absorbance is proportional to the palladium content, and quantification is performed by comparison with the standard […]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[36,77],"tags":[],"class_list":["post-3729","post","type-post","status-publish","format-standard","hentry","category-applications","category-bio-tech-and-life-sciences"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/posts\/3729","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/comments?post=3729"}],"version-history":[{"count":0,"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/posts\/3729\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/media?parent=3729"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/categories?post=3729"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/tags?post=3729"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}