{"id":3708,"date":"2025-07-10T14:49:45","date_gmt":"2025-07-10T06:49:45","guid":{"rendered":"https:\/\/www.pgeneral.com\/?p=3708"},"modified":"2025-07-10T14:49:45","modified_gmt":"2025-07-10T06:49:45","slug":"determination-of-lead-in-baijiu-graphite-furnace-atomic-absorption-spectrometry","status":"publish","type":"post","link":"https:\/\/www.pgeneral.com\/tr\/applications\/determination-of-lead-in-baijiu-graphite-furnace-atomic-absorption-spectrometry","title":{"rendered":"Baijiu'daki Kur\u015fun Belirlenmesi (Grafit F\u0131r\u0131n\u0131 Atom Emme Spektrometrisi)"},"content":{"rendered":"
    \n
  1. Y\u00f6ntem Genel Bak\u0131\u015f<\/li>\n<\/ol>\n

    Sindirimden sonra \u00f6rnek bir grafit f\u0131r\u0131n\u0131nda atomla\u015ft\u0131r\u0131l\u0131r ve emici 283,3 nm'de \u00f6l\u00e7\u00fcl\u00fcr. Belirli bir konsantrasyon aral\u0131\u011f\u0131nda, kur\u015fun emici i\u00e7eri\u011fine orant\u0131l\u0131d\u0131r ve standart bir seriyle kar\u015f\u0131la\u015ft\u0131r\u0131larak miktarl\u0131 analiz yap\u0131l\u0131r.<\/p>\n

      \n
    1. 2. Aletler ve Reagentler<\/li>\n<\/ol>\n

      2.1 Aletler ve Ekipmanlar<\/p>\n

      2.1.1 Tespit Aletleri<\/p>\n

      Grafit F\u0131r\u0131n Atom Emme Spektrofotometri (Kur\u015fun Bo\u015f Katod Lambas\u0131)<\/p>\n

      So\u011futma Sirk\u00fclasyon Su Sistemi (Pompa nominal ak\u0131\u015f\u0131: 3,5 L \/ dakika)<\/p>\n

      Argon Gaz\u0131 (% 99,99 safl\u0131k)<\/p>\n

      2.1.2 \u00d6n Tedavi Ekipmanlar\u0131<\/p>\n

      Mikropipetler 1 her 100\u03bcL ~ 1000\u03bcL, 1000\u03bcL ~ 5000\u03bcL, 5 ~ 50\u03bcL<\/p>\n

      Hacimli \u015fi\u015feler Birka\u00e7 10 mL, 100 mL<\/p>\n

      Ayarlanabilir Elektrikli S\u0131cak Plaka Nominal s\u0131cakl\u0131k: Oda s\u0131cakl\u0131\u011f\u0131 ~ 300 \u2103<\/p>\n

      Analitik Denge Hassasiyeti: 1 mg ve 0.1 mg<\/p>\n

      G\u00f6zl\u00fckler Birka\u00e7<\/p>\n

      Erlenmeyer \u015ei\u015feleri 150ml<\/p>\n

      2.2 Reagentler<\/p>\n

      2.2.1 Reagentler<\/p>\n

      Nitrik Asit (MOS s\u0131n\u0131f\u0131)<\/p>\n

        \n
      1. 3. Operasyon Prosed\u00fcrleri<\/li>\n<\/ol>\n

        3.1 \u00d6rnek Tedavi<\/p>\n

        3.1.1 Test \u00c7\u00f6z\u00fcm\u00fcn\u00fcn Haz\u0131rlanmas\u0131<\/p>\n

        \u00d6rne\u011fin 1 ~ 2 g'ini (0,001 g'e kadar do\u011fru) bir Erlenmeyer \u015fi\u015fesine do\u011fru bir \u015fekilde a\u011f\u0131rlay\u0131n, elektrikli bir s\u0131cak plaka \u00fczerinde kurulu\u011fa kadar buharla\u015f\u0131n, 10 mL nitrik asit ekleyin ve s\u0131cak plaka \u00fczerinde sindirin (referans ko\u015fullar\u0131: 80 \u00b0 C \/ 1 saat, 120 \u00b0 C \/ 1 saat, 140 \u00b0 C \/ 2 saat, 160 \u00b0 C \/ 2 saat, 180 \u00b0 C'ye kadar \u0131s\u0131t\u0131n). \u00c7\u00f6z\u00fcm kahverengi ise, daha fazla nitrik asit ekleyin ve beyaz duman yay\u0131lana kadar sindirin. \u00c7\u00f6z\u00fcm renksiz, \u015feffaf veya hafif sar\u0131 olmal\u0131d\u0131r. So\u011futuktan sonra, su ile hacimli bir \u015fi\u015fede 10 mL'ye seyreltin ve ayn\u0131 anda bir reaktif bo\u015flu\u011fu haz\u0131rlay\u0131n.<\/p>\n

        3.1.2 Standart \u00c7\u00f6z\u00fcmlerin Haz\u0131rlanmas\u0131<\/p>\n

        1) Kur\u015fun Standart Ara \u00c7\u00f6z\u00fcm\u00fcn\u00fcn Haz\u0131rlanmas\u0131 (1.0 \u03bcg \/ mL):<\/p>\n

        0,1 mL kur\u015fun ulusal standart \u00e7\u00f6z\u00fcm\u00fcn\u00fc 100 mL hacimli bir kolba do\u011fru bir \u015fekilde pipetleyin ve 1,0 \u03bcg\/mL'de kur\u015fun standart ara \u00e7\u00f6z\u00fcm\u00fcn\u00fc elde etmek i\u00e7in nitrik asit \u00e7\u00f6z\u00fcm\u00fc (99) ile i\u015farete kadar seyretin.<\/p>\n

          \n
        • Kur\u015fun Standart Serisinin Haz\u0131rlanmas\u0131:<\/li>\n<\/ul>\n

          0,0, 0,5, 1,0, 2,0, 3,0 mL kur\u015fun standart ara \u00e7\u00f6z\u00fcm\u00fcn\u00fc (1,0 \u03bcg\/mL) s\u0131ras\u0131yla 100 mL hacimli \u015fi\u015felere pipet edin ve 0,0, 5,0, 10,0, 20,0, 30,0 ng\/mL kur\u015fun standart serisi elde etmek i\u00e7in i\u015farete nitrik asit (1 99) ile seyreltin.<\/p>\n

          3.2 \u00d6rnek Testi<\/p>\n

          1) Test Ko\u015fullar\u0131<\/p>\n

          Grafit f\u0131r\u0131n atomik emim spektrofotometri i\u00e7in referans ko\u015fullar\u0131:<\/p>\n

          Dalga boyu 283.3 nm<\/p>\n

          Spektral Bant Geni\u015fli\u011fi 0.4 nm<\/p>\n

          Element Lamba Ak\u0131m\u0131 2.0 mA<\/p>\n

          Arka plan d\u00fczeltme y\u00f6ntemi deuterium lamba<\/p>\n

          Enjeksiyon hacmi 15 \u03bcL<\/p>\n

            \n
          • \u00d6rnek testi<\/li>\n<\/ul>\n

            Standart \u00e7\u00f6z\u00fcmlerin, bo\u015f test \u00e7\u00f6z\u00fcm\u00fcn\u00fcn ve test \u00e7\u00f6z\u00fcm\u00fcn\u00fcn emilimini s\u0131ras\u0131yla \u00f6l\u00e7\u00fcn. S\u0131f\u0131r standart \u00e7\u00f6z\u00fcm\u00fcn emilimini her standart \u00e7\u00f6z\u00fcm\u00fcn emiliminden \u00e7\u0131kar\u0131n, k\u00fctle konsantrasyonu absisa olarak ve ilgili emilimi ordinat olarak bir \u00e7al\u0131\u015fma e\u011frisi \u00e7izin. Test \u00e7\u00f6z\u00fcm\u00fcndeki kur\u015fun k\u00fctle konsantrasyonunu \u00f6l\u00e7\u00fclen emici oran\u0131na dayanarak \u00e7al\u0131\u015fma e\u011frisinden belirleyin.<\/p>\n

            3.3 Sonu\u00e7 Hesaplamas\u0131<\/p>\n

            \"\"<\/p>\n

            Nerede:<\/p>\n

            X: \u00d6rnekteki kur\u015fun i\u00e7eri\u011fi, kilogram ba\u015f\u0131na miligram (mg\/kg) olarak;<\/p>\n

            \u03c1: \u00d6rnek \u00e7\u00f6z\u00fcm\u00fcndeki kur\u015fun k\u00fctle konsantrasyonu, mililitre ba\u015f\u0131na nanogramlarda (ng\/mL);<\/p>\n

            \u03c10: Bo\u015f \u00e7\u00f6z\u00fcmdeki kur\u015fun k\u00fctle konsantrasyonu, mililitre ba\u015f\u0131na nanogram (ng\/mL);<\/p>\n

            V: Sabit hacmdan sonra \u00f6rnek sindirim \u00e7\u00f6z\u00fcm\u00fcn\u00fcn hacmi, mililitrelerde (mL);<\/p>\n

            m: \u00d6rnek a\u011f\u0131rl\u0131\u011f\u0131, gram (g);<\/p>\n

            1000: D\u00f6n\u00fc\u015f\u00fcm katsay\u0131s\u0131.<\/p>","protected":false},"excerpt":{"rendered":"

            Method Overview After digestion, the sample is atomized in a graphite furnace, and the absorbance is measured at 283.3 nm. Within a certain concentration range, the absorbance of lead is proportional to its content, and quantitative analysis is performed by comparison with a standard series. 2. Instruments and Reagents 2.1 Instruments and Equipment 2.1.1 Detection […]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[36,73],"tags":[],"class_list":["post-3708","post","type-post","status-publish","format-standard","hentry","category-applications","category-food-and-drugs"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/posts\/3708","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/comments?post=3708"}],"version-history":[{"count":0,"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/posts\/3708\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/media?parent=3708"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/categories?post=3708"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/tags?post=3708"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}