{"id":2799,"date":"2023-09-14T13:29:59","date_gmt":"2023-09-14T05:29:59","guid":{"rendered":"http:\/\/www.pgeneral.com\/?p=2799"},"modified":"2023-09-14T13:30:06","modified_gmt":"2023-09-14T05:30:06","slug":"baldaki%cc%87-di%cc%87yastaz-sayisinin-uv-vis-spektrofotometresi%cc%87-i%cc%87le-beli%cc%87rlenmesi%cc%87-i%cc%87ci%cc%87n-yontem","status":"publish","type":"post","link":"https:\/\/www.pgeneral.com\/tr\/applications\/food-and-drugs\/method-for-the-determination-of-diastase-number-in-honey-by-uv-vis-spectrophotometer","title":{"rendered":"UV-Vis spektrofotometre ile balda diyastaz say\u0131s\u0131n\u0131n belirlenmesi y\u00f6ntemi"},"content":{"rendered":"

\u0130lke
\nNi\u015fasta \u00e7\u00f6zeltisini bal \u00f6rnek \u00e7\u00f6zeltisine koyduktan sonra, baz\u0131 ni\u015fasta balda diyastaz ile hidrolizdir.<\/p>\n

Reaktifler ve Malzeme
\nSpesifik bildirimi olanlar hari\u00e7, AR derecesi reaktifleri kullan\u0131l\u0131r;<\/p>\n

Reaktif:
\n1. iyot.
\n2. Potasyum iyod\u00fcr.
\n3. Sodyum asetat (CH3Coona \u00b7 3H2O).
\n4. Buzlu asetat asidi
\n5. Sodyum klor\u00fcr.
\n6. \u00c7\u00f6z\u00fcn\u00fcr ni\u015fasta.
\n7. \u0130yot stok \u00e7\u00f6zeltisi: 30ml ~ 40ml su ile kar\u0131\u015ft\u0131r\u0131lan 8.8g iyot ve 22G potasyum iyod\u00fcr tart\u0131n, 1000 ml'ye kadar seyreltin.
\n8. \u0130yot \u00e7\u00f6zeltisi: 20g potasyum iyot tart\u0131n, 5ml iyot stok \u00e7\u00f6zeltisi ekleyin, su ile 500ml'ye seyreltin (bu \u00e7\u00f6zelti sadece 2 g\u00fcn kullan\u0131labilir).
\n9. Asetat tampon \u00e7\u00f6zeltisi: pH 5,3 (1,59 mol\/L). 87 g Sodyum Asetat tart\u0131n ve 400 mL suda seyreltin. 10,5 mL buzlu asetat asit ekleyin ve 500 mL'ye seyreltin. Gerekirse, pH de\u011ferini 5,3'e ayarlamak i\u00e7in sodyum asetat veya asetat asit kullan\u0131n.
\n10. Sodyum klor\u00fcr \u00e7\u00f6zeltisi (0,5 mol\/L): 14,5 g NaCl'yi tart\u0131n, 500 mL suda \u00e7\u00f6z\u00fcn.
\n11. Ni\u015fasta \u00c7\u00f6zelti: 2G'yi 90ml suda \u00e7\u00f6z\u00fcn\u00fcr ni\u015fastay\u0131 \u00e7\u00f6z\u00fcn.<\/p>\n

Te\u00e7hizat:
\n1. UV-Vis spektrofotometre.
\n2. Sabit s\u0131cakl\u0131k h\u00fccre tutucusu<\/p>\n

\u00d6rnek haz\u0131rlama ve koruma
\n\u00d6rnek haz\u0131rlama:
\nNumuneyi iyice kar\u0131\u015ft\u0131r\u0131n, ard\u0131ndan test numunesi olarak 0.5kg bir numune \u015fi\u015fesine aktar\u0131n.
\nNot: Kristal g\u00f6r\u00fcn\u00fcp g\u00f6r\u00fcnm\u00fcyor olsun, t\u00fcm numune \u0131s\u0131t\u0131lamaz.<\/p>\n

\u00d6rnek Koruma:
\nNumune oda s\u0131cakl\u0131\u011f\u0131 alt\u0131nda korunabilir.<\/p>\n

Deney prosed\u00fcr\u00fc
\nStandart Ni\u015fasta \u00c7\u00f6z\u00fcm Testi
\n5.0 ml ni\u015fasta \u00e7\u00f6zeltisi (reaktif 11) ve 10.0 ml su \u00f6l\u00e7\u00fcn, 40 \u2103 su banyosuna 15 dakika ayr\u0131 ayr\u0131 yerle\u015ftirin.<\/p>\n

\u00d6rnek Testi:
\n1. Spektrofotometre ko\u015fullar\u0131:
\nA) Dalga boyu: 660Nm
\nb) Referans: Su
\n2. Numune \u00f6n i\u015flem: 5G (\u00b1 0.01g) haz\u0131rlanan numune \u00f6l\u00e7\u00fcn ve 20ml'lik bir behere aktar\u0131n.
\n3. 5ml ni\u015fasta \u00e7\u00f6zeltisi, 10ml numune \u00e7\u00f6zeltisi (numune testi 2) ve 10ml iyot \u00e7\u00f6zeltisini \u00f6l\u00e7\u00fcn, 40 \u2103 su banyosuna 15 dakika ayr\u0131 ayr\u0131 yerle\u015ftirin.
\n4. 5 dakika sonra 1.0 ml kar\u0131\u015f\u0131k \u00e7\u00f6zeltiyi 10 mL \u0131s\u0131t\u0131lm\u0131\u015f iyot \u00e7\u00f6zeltisine aktar\u0131n ve kaydedilen miktarda su (standart ni\u015fasta \u00e7\u00f6zeltisi a\u015famas\u0131nda kaydedildi) ile seyreltin ve tekrar iyice kar\u0131\u015ft\u0131r\u0131n.
\n5. Absorbans 0.235'ten b\u00fcy\u00fckse, absorbans 0.235'ten az olana kadar 4. ad\u0131m\u0131n tekrarlanmas\u0131 gerekir.
\n6. \u00d6l\u00e7\u00fcm s\u0131ras\u0131nda kar\u0131\u015f\u0131k \u00e7\u00f6zelti, iyot \u00e7\u00f6zeltisi ve su 40 su banyosunda tutulmal\u0131d\u0131r.<\/p>\n

Hesaplama
\nBir logaritmik koordinat ka\u011f\u0131d\u0131nda, Absorbans (%) olarak Y eksenini ve zaman (min) olarak X ekseni ad\u0131n\u0131.<\/p>\n

\"\"<\/p>\n

Burada x \u00e7\u00f6zeltideki diyastaz say\u0131s\u0131d\u0131r (ml\/(g \u00b7 h)), t kar\u015f\u0131l\u0131k gelen s\u00fcre (min).<\/p>\n

Sonu\u00e7lar
\nBu y\u00f6ntem kullan\u0131larak, deneyin tekrarlanabilirli\u011fi ve tekrarlanabilirli\u011fi \u2265'tir.<\/p>","protected":false},"excerpt":{"rendered":"

Principle After putting starch solution into honey sample solution, some starch is hydrolysis by diastase in honey. The starch residue appears as bluish violet color after mixing with iodine. As the reaction process, the color disappears finally. By using UV-Vis Spectrophotometer at 660nm wavelength, the time required to reach the specific absorbance can be measured. […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[73],"tags":[],"class_list":["post-2799","post","type-post","status-publish","format-standard","hentry","category-food-and-drugs"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/posts\/2799","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/comments?post=2799"}],"version-history":[{"count":0,"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/posts\/2799\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/media?parent=2799"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/categories?post=2799"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.pgeneral.com\/tr\/wp-json\/wp\/v2\/tags?post=2799"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}