{"id":4879,"date":"2026-08-13T13:51:10","date_gmt":"2026-08-13T05:51:10","guid":{"rendered":"https:\/\/www.pgeneral.com\/?p=4879"},"modified":"2026-08-13T13:51:10","modified_gmt":"2026-08-13T05:51:10","slug":"%e3%80%90application-case-of-l820-liquid-chromatograph%e3%80%91-protocol-in-pharmacopoeia-of-the-peoples-republic-of-china-2025-edition-determination-of-ginsenoside-rg1-ginsen","status":"publish","type":"post","link":"https:\/\/www.pgeneral.com\/pt\/applications\/%e3%80%90application-case-of-l820-liquid-chromatograph%e3%80%91-protocol-in-pharmacopoeia-of-the-peoples-republic-of-china-2025-edition-determination-of-ginsenoside-rg1-ginsen","title":{"rendered":"# \u3010Caso de Aplica\u00e7\u00e3o do Cromat\u00f3grafo L\u00edquido L820\u3011 Protocolo da Farmacopeia da Rep\u00fablica Popular da China (Edi\u00e7\u00e3o de 2025) \u2014 Determina\u00e7\u00e3o de Ginsenos\u00eddeo Rg1, Ginsenos\u00eddeo Rb1 e Notoginsenos\u00eddeo R1 em Notoginseng Radix et Rhizoma"},"content":{"rendered":"

Protocolo na Farmacopeia da Rep\u00fablica Popular da China (Edi\u00e7\u00e3o de 2025) \u2014 Determina\u00e7\u00e3o de Ginsenos\u00eddeo Rg1, Ginsenos\u00eddeo Rb1 e Notoginsenos\u00eddeo R1 em Notoginseng Radix et Rhizoma<\/p>\n

A an\u00e1lise de Notoginseng Radix et Rhizoma \u00e9 um item revisado no Farmacopeia da Rep\u00fablica Popular da China (Edi\u00e7\u00e3o de 2025)<\/em>, em que o procedimento de elui\u00e7\u00e3o \u00e9 ajustado de um gradiente de dois segmentos para um gradiente de quatro segmentos. De acordo com as condi\u00e7\u00f5es cromatogr\u00e1ficas especificadas na Farmacopeia Chinesa de 2025, foi estabelecido um m\u00e9todo para a determina\u00e7\u00e3o de ginsenos\u00eddeo Rg1, ginsenos\u00eddeo Rb1 e notoginsenos\u00eddeo R1 em Notoginseng Radix et Rhizoma, utilizando uma coluna cromatogr\u00e1fica C18.<\/p>\n

Os resultados dos testes de repetibilidade na solu\u00e7\u00e3o padr\u00e3o de refer\u00eancia s\u00e3o os seguintes: o desvio padr\u00e3o relativo (RSD) do tempo de reten\u00e7\u00e3o para ginsenos\u00eddeo Rg1, ginsenos\u00eddeo Rb1 e notoginsenos\u00eddeo R1 \u00e9 \u2264 0,155%; o RSD da \u00e1rea do pico \u00e9 \u2264 0,099%; os fatores de cauda variam de 0,882 a 1,047; o n\u00famero de pratos te\u00f3ricos do notoginsenos\u00eddeo R1 atinge 7867, o que atende aos requisitos do padr\u00e3o de refer\u00eancia. Farmacopeia da Rep\u00fablica Popular da China (Edi\u00e7\u00e3o de 2025)<\/em>Este m\u00e9todo pode servir de refer\u00eancia para a an\u00e1lise quantitativa dos ginsenos\u00eddeos Rg1, Rb1 e R1 notoginsenos\u00eddeos em Notoginseng Radix et Rhizoma.<\/p>\n

I. Condi\u00e7\u00f5es do instrumento<\/h2>\n

\u2022 Cromat\u00f3grafo L\u00edquido de Alta Efici\u00eancia Persee L820<\/p>\n

    \n
  • Bomba bin\u00e1ria de gradiente de alta press\u00e3o L820-P22<\/li>\n
  • Amostrador autom\u00e1tico L820-A11<\/li>\n
  • Forno de coluna L820-C11<\/li>\n
  • Detector UV-Vis L820-D10<\/li>\n
  • Esta\u00e7\u00e3o de trabalho padr\u00e3o para cromatografia LCWin V5.0<\/li>\n
  • Bandeja de solvente L820-C304100001<\/li>\n<\/ul>","protected":false},"excerpt":{"rendered":"

    Protocol in Pharmacopoeia of the People\u2019s Republic of China (2025 Edition) \u2014 Determination of Ginsenoside Rg1, Ginsenoside Rb1 and Notoginsenoside R1 in Notoginseng Radix et Rhizoma The assay of Notoginseng Radix et Rhizoma is a revised item in the Pharmacopoeia of the People\u2019s Republic of China (2025 Edition), in which the elution procedure is adjusted […]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[36,77],"tags":[],"class_list":["post-4879","post","type-post","status-publish","format-standard","hentry","category-applications","category-bio-tech-and-life-sciences"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.pgeneral.com\/pt\/wp-json\/wp\/v2\/posts\/4879","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.pgeneral.com\/pt\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.pgeneral.com\/pt\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/pt\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/pt\/wp-json\/wp\/v2\/comments?post=4879"}],"version-history":[{"count":1,"href":"https:\/\/www.pgeneral.com\/pt\/wp-json\/wp\/v2\/posts\/4879\/revisions"}],"predecessor-version":[{"id":4881,"href":"https:\/\/www.pgeneral.com\/pt\/wp-json\/wp\/v2\/posts\/4879\/revisions\/4881"}],"wp:attachment":[{"href":"https:\/\/www.pgeneral.com\/pt\/wp-json\/wp\/v2\/media?parent=4879"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.pgeneral.com\/pt\/wp-json\/wp\/v2\/categories?post=4879"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.pgeneral.com\/pt\/wp-json\/wp\/v2\/tags?post=4879"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}