{"id":3805,"date":"2025-08-01T11:09:02","date_gmt":"2025-08-01T03:09:02","guid":{"rendered":"https:\/\/www.pgeneral.com\/?p=3805"},"modified":"2025-08-01T11:20:01","modified_gmt":"2025-08-01T03:20:01","slug":"determination-of-copper-in-graphite-powder-flame-atomic-absorption-spectrometry","status":"publish","type":"post","link":"https:\/\/www.pgeneral.com\/pt\/applications\/determination-of-copper-in-graphite-powder-flame-atomic-absorption-spectrometry\/","title":{"rendered":"Determina\u00e7\u00e3o de cobre em p\u00f3 de grafito – Espectrometria de Absor\u00e7\u00e3o At\u00f4mica de Flame"},"content":{"rendered":"

1 Method Overview<\/h2>\n
After the sample is digested with aqua regia, the determination is carried out using an air-acetylene flame at a wavelength of 324.7 nm with the instrument.<\/div>\n
\n

2 Instruments and Reagents<\/h2>\n

2.1 Instruments and Equipment<\/h3>\n

2.1.1 Detection Instruments<\/h4>\n
\n
\n\n\n\n\n\n\n\n
Serial Number<\/th>\nName<\/th>\nQuantity<\/th>\nTechnical Requirements<\/th>\nAccessories<\/th>\n<\/tr>\n<\/thead>\n
1<\/td>\nFlame Atomic Absorption Spectrophotometer<\/td>\n1 set<\/td>\n<\/td>\nCopper hollow cathode lamp<\/td>\n<\/tr>\n
2<\/td>\nAcetylene<\/td>\n1 cylinder<\/td>\nPurity \u2265 99.99%<\/td>\n<\/td>\n<\/tr>\n
3<\/td>\nAir Compressor<\/td>\n1 set<\/td>\nRated exhaust pressure: 0.3 MPa<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n

2.1.2 Pretreatment Equipment<\/h4>\n
\n
\n\n\n\n\n\n\n\n\n\n\n
Serial Number<\/th>\nName<\/th>\nQuantity<\/th>\nTechnical Requirements<\/th>\nAccessories<\/th>\n<\/tr>\n<\/thead>\n
1<\/td>\nTemperature-controlled Hot Plate<\/td>\n1 set<\/td>\nRated temperature: room temperature ~ 300\u2103<\/td>\n<\/td>\n<\/tr>\n
2<\/td>\nElectronic Balance<\/td>\n1 set<\/td>\nSensitivity 0.1 mg<\/td>\n<\/td>\n<\/tr>\n
3<\/td>\nUltrapure Water Device<\/td>\n1 set<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
4<\/td>\nMicropipette<\/td>\n1 each<\/td>\n20\u03bcL ~ 200\u03bcL; 100\u03bcL ~ 1000\u03bcL<\/td>\n<\/td>\n<\/tr>\n
5<\/td>\nVolumetric Flask<\/td>\nSeveral<\/td>\n100 mL<\/td>\n<\/td>\n<\/tr>\n
6<\/td>\nTest Tube<\/td>\nSeveral<\/td>\n25 mL<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n

2.2 Reagents<\/h3>\n

2.2.1 Reagents<\/h4>\n
\n
\n\n\n\n\n\n\n
Serial Number<\/th>\nName<\/th>\nTechnical Requirements<\/th>\nRemarks<\/th>\n<\/tr>\n<\/thead>\n
1<\/td>\nNitric Acid<\/td>\nMOS grade<\/td>\n<\/td>\n<\/tr>\n
2<\/td>\nHydrochloric Acid<\/td>\nGuaranteed reagent<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n

2.2.2 Prepared Reagents<\/h4>\n
\n
\n\n\n\n\n\n
Serial Number<\/th>\nName<\/th>\nPreparation Method<\/th>\nRemarks<\/th>\n<\/tr>\n<\/thead>\n
1<\/td>\n(1+99) Nitric Acid Solution<\/td>\nMeasure 10 mL of nitric acid, slowly add to 990 mL of deionized water, and mix well<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n

2.3 Standards<\/h3>\n

2.3.1 Stock Solutions<\/h4>\n
\n
\n\n\n\n\n\n
Serial Number<\/th>\nNumber<\/th>\nName<\/th>\nTechnical Requirements<\/th>\nRemarks<\/th>\n<\/tr>\n<\/thead>\n
1<\/td>\nGBW08615<\/td>\nCopper Single Element Solution Standard Substance<\/td>\n1000\u03bcg\/mL<\/td>\nNational Institute of Metrology, China<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n

3 Operation Process<\/h2>\n

3.1 Sample Processing<\/h3>\n

3.1.1 Preparation of Test Solution<\/h4>\n
Weigh 0.25 g of the sample, accurate to 0.00001 g, into a digestion tank. Add 3 mL of concentrated nitric acid and 9 mL of concentrated hydrochloric acid, cover and tighten the digestion tank, and put it into a microwave digestion instrument for digestion. Prepare a blank in the same way. After cooling, filter and dilute to volume in a 25 mL volumetric flask, and put it on the machine for testing.<\/div>\n

 <\/p>\n

Microwave Heating Program<\/div>\n

 <\/p>\n

\n
\n\n\n\n\n\n\n\n\n\n
Step<\/th>\nTemperature\/\u2103<\/th>\nHolding Time\/min<\/th>\n<\/tr>\n<\/thead>\n
1<\/td>\n100<\/td>\n3<\/td>\n<\/tr>\n
2<\/td>\n120<\/td>\n3<\/td>\n<\/tr>\n
3<\/td>\n140<\/td>\n3<\/td>\n<\/tr>\n
4<\/td>\n160<\/td>\n3<\/td>\n<\/tr>\n
5<\/td>\n180<\/td>\n30<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n

3.1.2 Preparation of Standard Solution<\/h4>\n
    \n
  1. Preparation of copper standard series: Accurately pipette 0.0, 0.025, 0.05, 0.1, and 0.2 mL of copper standard solution (1000\u03bcg\/mL) into 100 mL volumetric flasks respectively, dilute to the mark with (1+99) nitric acid, and mix well. The mass concentrations of this standard series solution are 0.0, 0.25, 0.5, 1.0, and 2.0 \u03bcg\/mL respectively.<\/li>\n<\/ol>\n

    3.2 Sample Testing<\/h3>\n
      \n
    1. Test Conditions Reference Conditions for Flame Atomic Absorption Spectrophotometer Detection<\/li>\n<\/ol>\n

       <\/p>\n

      \n
      \n\n\n\n\n\n\n\n\n\n\n\n
      Element<\/th>\nCopper<\/th>\n<\/tr>\n<\/thead>\n
      Wavelength (nm)<\/td>\n324.7<\/td>\n<\/tr>\n
      Spectral Bandwidth (nm)<\/td>\n0.4<\/td>\n<\/tr>\n
      Element Lamp Current (mA)<\/td>\n3.0<\/td>\n<\/tr>\n
      Fuel Gas Height (mm)<\/td>\n6<\/td>\n<\/tr>\n
      Fuel Gas Flow Rate (mL\/min)<\/td>\n1400<\/td>\n<\/tr>\n
      Air Compressor Pressure (MPa)<\/td>\n0.22<\/td>\n<\/tr>\n
      Background Correction Method<\/td>\nNone<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n

       <\/p>\n

        \n
      1. Sample Testing Adjust the instrument to the best working state, ignite the flame, adjust the fuel gas flow rate, test the standard and sample solutions in sequence, read the data, input the sample weight, and check and record the results.<\/li>\n<\/ol>\n

        3.3 Result Calculation<\/h3>\n
        \"\"<\/div>\n

         <\/p>\n

        Where:<\/div>\n
        X — Copper content in the sample, unit is milligram per kilogram (mg\/kg);<\/div>\n
        \u03c1 — Mass concentration of copper in the sample solution to be tested, microgram per milliliter (\u03bcg\/mL);<\/div>\n
        \u03c1\u2080– Mass concentration of copper in the blank solution, microgram per milliliter (\u03bcg\/mL);<\/div>\n
        f — Dilution factor of the sample digestion solution;<\/div>\n
        V — Total volume of the sample digestion solution, unit is milliliter (mL);<\/div>\n
        m — Mass of the sample, unit is gram (g);<\/div>\n<\/div>\n