{"id":2799,"date":"2023-09-14T13:29:59","date_gmt":"2023-09-14T05:29:59","guid":{"rendered":"http:\/\/www.pgeneral.com\/?p=2799"},"modified":"2023-09-14T13:30:06","modified_gmt":"2023-09-14T05:30:06","slug":"uv-vis-%eb%b6%84%ea%b4%91%ea%b4%91%eb%8f%84%ea%b3%84%eb%a1%9c-%ea%bf%80%ec%9d%98-%eb%94%94%ec%95%84%ec%8a%a4%ed%83%80%ec%a0%9c-%ec%88%98%eb%a5%bc-%ec%b8%a1%ec%a0%95%ed%95%98%eb%8a%94-%eb%b0%a9","status":"publish","type":"post","link":"https:\/\/www.pgeneral.com\/ko\/applications\/food-and-drugs\/method-for-the-determination-of-diastase-number-in-honey-by-uv-vis-spectrophotometer","title":{"rendered":"UV-Vis \ubd84\uad11 \uad11\ub3c4\uacc4\uc5d0 \uc758\ud55c \uafc0\uc5d0\uc11c\uc758 \ub514\uc544\uc2a4\ud2b8 \ud0c0\uc81c \uc218\uc758 \uacb0\uc815 \ubc29\ubc95"},"content":{"rendered":"

\uc6d0\uce59
\n\uc804\ubd84 \uc6a9\uc561\uc744 \uafc0 \uc0d8\ud50c \uc6a9\uc561\uc5d0 \ub123\uc740 \ud6c4, \uc77c\ubd80 \uc804\ubd84\uc740 \uafc0\uc5d0\uc11c \ub514\uc544\uc2a4\ud2b8 \ud0c0\uc81c\uc5d0 \uc758\ud55c \uac00\uc218 \ubd84\ud574\uc785\ub2c8\ub2e4.<\/p>\n

\uc2dc\uc57d \ubc0f \uc7ac\ub8cc
\n\ud2b9\uc815 \uc54c\ub9bc\uc744\ubc1b\uc740 \uac83\uc744 \uc81c\uc678\ud558\uace0 AR\ub3c4 \uc2dc\uc57d\uc774 \uc0ac\uc6a9\ub429\ub2c8\ub2e4.<\/p>\n

\uc2dc\uc57d:
\n1. \uc694\uc624\ub4dc.
\n2. \uc694\uc624\ub4dc\ud654 \uce7c\ub968.
\n3. \ub098\ud2b8\ub968 \uc544\uc138\ud14c\uc774\ud2b8 (CH3coona \u00b7 3H2O).
\n4. \uc544\uc774\uc2a4 \uc544\uc138\ud14c\uc774\ud2b8 \uc0b0
\n5. \uc5fc\ud654\ub098\ud2b8\ub968.
\n6. \uac00\uc6a9\uc131 \uc804\ubd84.
\n\uc694\uc624\ub4dc \uc2a4\ud1a1 \uc6a9\uc561 : 8.8g \uc694\uc624\ub4dc\uc640 22g\uc758 \uc694\uc624\ub4dc\ud654 \uce7c\ub968\uc758 \ubb34\uac8c\ub85c 30ml ~ 40ml \ubb3c\uacfc \ud63c\ud569\ub418\uc5b4 1000ml\ub85c \ud76c\uc11d\ud569\ub2c8\ub2e4.
\n8. \uc694\uc624\ub4dc \uc6a9\uc561 : 20g\uc758 \uce7c\ub968 \uc694\uc624\ub4dc \uccb4\uc911, 5ml \uc694\uc624\ub4dc \uc2a4\ud1a1 \uc6a9\uc561\uc744 \ucca8\uac00\ud558\uace0 \ubb3c\uc5d0 \uc758\ud574 500ml\uc5d0 \ud76c\uc11d\ub429\ub2c8\ub2e4 (\uc774 \uc6a9\uc561\uc740 2 \uc77c \ub3d9\uc548 \ub9cc \uc0ac\uc6a9\ud560 \uc218 \uc788\uc74c).
\n9. \uc544\uc138\ud2b8\uc0b0 \uc644\ucda9\uc561: pH 5.3 (1.59 mol\/L). \uc544\uc138\ud2b8\uc0b0\ub098\ud2b8\ub968 87g\uc744 \ub2ec\uc544 \ubb3c 400mL\uc5d0 \ud76c\uc11d\ud55c\ub2e4. \uc5bc\uc74c \uc544\uc138\ud2b8\uc0b0 10.5mL\ub97c \ub123\uace0 500mL\ub85c \ud76c\uc11d\ud55c\ub2e4. \ud544\uc694\ud55c \uacbd\uc6b0 \uc544\uc138\ud2b8\uc0b0\ub098\ud2b8\ub968\uc774\ub098 \uc544\uc138\ud2b8\uc0b0\uc744 \uc0ac\uc6a9\ud558\uc5ec pH\ub97c 5.3\uc73c\ub85c \uc870\uc808\ud55c\ub2e4.
\n10. \uc5fc\ud654\ub098\ud2b8\ub968 \uc6a9\uc561(0.5 mol\/L): NaCl 14.5g\uc744 \ub2ec\uc544 \ubb3c 500mL\uc5d0 \ub179\uc785\ub2c8\ub2e4.
\n\uc804\ubd84 \uc6a9\uc561 : 90ml \ubb3c\uc5d0 2G \uac00\uc6a9\uc131 \uc804\ubd84\uc744 \ub179\uc785\ub2c8\ub2e4.<\/p>\n

\uc7a5\ube44:
\n1. UV-Vis \ubd84\uad11 \uad11\ub3c4\uacc4.
\n2. \uc77c\uc815\ud55c \uc628\ub3c4 \uc138\ud3ec \ud640\ub354<\/p>\n

\uc0d8\ud50c \uc900\ube44 \ubc0f \ubcf4\uc874
\n\uc0d8\ud50c \uc900\ube44 :
\n\uc0d8\ud50c\uc744 \uc798 \uc800\uc5b4 \uc900 \ub2e4\uc74c 0.5kg\uc744 \ud14c\uc2a4\ud2b8 \uc0d8\ud50c\ub85c \uc0d8\ud50c \ubcd1\uc73c\ub85c \uc62e\uae41\ub2c8\ub2e4.
\n\ucc38\uace0 : \uacb0\uc815\uc774 \ub098\ud0c0\ub098\ub4e0 \uc544\ub2c8\ub4e0 \ubaa8\ub4e0 \uc0d8\ud50c\uc744 \uac00\uc5f4 \ud560 \uc218\ub294 \uc5c6\uc2b5\ub2c8\ub2e4.<\/p>\n

\uc0d8\ud50c \ubcf4\uc874 :
\n\uc0d8\ud50c\uc740 \uc2e4\uc628\uc5d0\uc11c \ubcf4\uc874 \ub420 \uc218 \uc788\uc2b5\ub2c8\ub2e4.<\/p>\n

\uc2e4\ud5d8 \uc808\ucc28
\n\uc804\ubd84 \uc194\ub8e8\uc158\uc758 \ud45c\uc900 \ud14c\uc2a4\ud2b8
\n5.0 ml\uc758 \uc804\ubd84 \uc6a9\uc561 (\uc2dc\uc57d 11) \ubc0f 10.0 ml\uc758 \ubb3c\uc744 \uce21\uc815\ud558\uace0, 40 \u2103 \uc218\uc870\uc5d0 15 \ubd84 \ub3d9\uc548 \uac1c\ubcc4\uc801\uc73c\ub85c \ubc30\uce58\ud569\ub2c8\ub2e4.<\/p>\n

\uc0d8\ud50c \ud14c\uc2a4\ud2b8 :
\n1. \ubd84\uad11 \uad11\ub3c4\uacc4 \uc870\uac74 :
\na) \ud30c\uc7a5 : 660nm
\nb) \ucc38\uc870 : \ubb3c
\n2. \uc0d8\ud50c \uc804\ucc98\ub9ac : 5G (\u00b1 0.01g) \uc900\ube44\ub41c \uc0d8\ud50c\uc744 \uce21\uc815\ud558\uace0 20ml \ube44\uc774\ucee4\ub85c \uc62e\uae41\ub2c8\ub2e4.
\n3. 5ML \uc804\ubd84 \uc6a9\uc561, 10ml \uc0d8\ud50c \uc6a9\uc561 (\uc0d8\ud50c \ud14c\uc2a4\ud2b8 2) \ubc0f 10ml \uc694\uc624\ub4dc \uc6a9\uc561\uc744 \uce21\uc815\ud558\uace0, 40 \u2103 \uc218\uc870\uc5d0 15 \ubd84 \ub3d9\uc548 \uac1c\ubcc4\uc801\uc73c\ub85c \ubc30\uce58\ud569\ub2c8\ub2e4.
\n4. 5 \ubd84 \ud6c4, 1.0ml \ud63c\ud569 \uc6a9\uc561\uc744 10ml \uac00\uc5f4 \ub41c \uc694\uc624\ub4dc \uc6a9\uc561\uc73c\ub85c \uc62e\uae30\uace0 \uae30\ub85d \ub41c \uc591\uc758 \ubb3c (\uc804\ubd84 \uc6a9\uc561 \ub2e8\uacc4\uc758 \ud45c\uc900 \uc2dc\ud5d8\uc5d0\uc11c \uae30\ub85d)\uc73c\ub85c \ud76c\uc11d\ud558\uace0 \ub2e4\uc2dc \uc798 \uc11e\ub294\ub2e4.
\n5. \ud761\uad11\ub3c4\uac00 0.235\ubcf4\ub2e4 \ud070 \uacbd\uc6b0 \ud761\uad11\ub3c4\uac00 0.235\ubcf4\ub2e4 \uc791\uc744 \ub54c\uae4c\uc9c0 4 \ub2e8\uacc4\ub97c \ubc18\ubcf5\ud574\uc57c\ud569\ub2c8\ub2e4.
\n6. \uce21\uc815 \uc911\uc5d0 \ud63c\ud569 \uc6a9\uc561, \uc694\uc624\ub4dc \uc6a9\uc561 \ubc0f \ubb3c\uc744 40 \u2103 \uc218\uc870\uc5d0\uc11c \uc720\uc9c0\ud574\uc57c\ud569\ub2c8\ub2e4.<\/p>\n

\uacc4\uc0b0
\n\ub85c\uadf8 \uc88c\ud45c \uc6a9\uc9c0\uc5d0\uc11c y \ucd95 \uc774\ub984 \ud761\uad11\ub3c4 (%), x \ucd95\uc73c\ub85c \uc2dc\uac04 (\ucd5c\uc18c)\uc73c\ub85c \uc774\ub984\uc744 \uc9c0\uc815\ud558\uc2ed\uc2dc\uc624.<\/p>\n

\"\"<\/p>\n

\uc5ec\uae30\uc11c x\ub294 \uc6a9\uc561 (ml\/(g \u00b7 h))\uc758 \ub514\uc544\uc2a4\ud2b8 \ub77c\uc81c \uc218\uc785\ub2c8\ub2e4. t\ub294 \ud574\ub2f9 \uc2dc\uac04 (\ucd5c\uc18c)\uc785\ub2c8\ub2e4.<\/p>\n

\uacb0\uacfc
\n\uc774 \ubc29\ubc95\uc744 \uc0ac\uc6a9\ud568\uc73c\ub85c\uc368 \uc2e4\ud5d8\uc758 \ubc18\ubcf5\uc131 \ubc0f \uc7ac\ud604\uc131\uc740 \u226595%\uc785\ub2c8\ub2e4.<\/p>","protected":false},"excerpt":{"rendered":"

Principle After putting starch solution into honey sample solution, some starch is hydrolysis by diastase in honey. The starch residue appears as bluish violet color after mixing with iodine. As the reaction process, the color disappears finally. By using UV-Vis Spectrophotometer at 660nm wavelength, the time required to reach the specific absorbance can be measured. […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[73],"tags":[],"class_list":["post-2799","post","type-post","status-publish","format-standard","hentry","category-food-and-drugs"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.pgeneral.com\/ko\/wp-json\/wp\/v2\/posts\/2799","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.pgeneral.com\/ko\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.pgeneral.com\/ko\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/ko\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/ko\/wp-json\/wp\/v2\/comments?post=2799"}],"version-history":[{"count":0,"href":"https:\/\/www.pgeneral.com\/ko\/wp-json\/wp\/v2\/posts\/2799\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.pgeneral.com\/ko\/wp-json\/wp\/v2\/media?parent=2799"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.pgeneral.com\/ko\/wp-json\/wp\/v2\/categories?post=2799"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.pgeneral.com\/ko\/wp-json\/wp\/v2\/tags?post=2799"}],"curies":[{"name":"WP","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}