\uc2e4\ud5d8 \ubaa9\ud45c
\n\uc2e4\ud5d8\uc801\uc73c\ub85c \ud06c\ub85c\ub9c8\ud1a0 \uadf8\ub798\ud53c \ubd84\uc11d\uc758 \uae30\ubcf8 \uc6d0\ub9ac\uc640 \uae30\uae30\uc758 \uae30\ubcf8 \uc791\ub3d9\uc744 \ubc30\uc6b0\uace0 \ub370\uc774\ud130 \ubd84\uc11d \ud504\ub85c\uc138\uc2a4\ub97c \uc774\ud574\ud558\uc2ed\uc2dc\uc624.
\n\ud06c\ub85c\ub9c8\ud1a0 \uadf8\ub798\ud53c \uc815\ub7c9\uc801 \ubc29\ubc95\uc5d0 \ub300\ud574 \ubc30\uc6b0\uace0 \ub2e4\ub978 \ubc29\ubc95\uc758 \uc7a5\uc810\uacfc \ub2e8\uc810\uc744 \ube44\uad50\ud558\uc2ed\uc2dc\uc624.<\/p>\n
HPLC \uc18c\uac1c
\n\uace0\uc18d : HPLC\ub294 \uace0\uc555 \uc8fc\uc785 \uc7a5\ube44\ub97c \uc0ac\uc6a9\ud558\uace0 \uc720\ub7c9\uc774 \ud06c\uac8c \uc99d\uac00\ud558\uba70 \ubd84\uc11d \uc18d\ub3c4\ub294 \ub9e4\uc6b0 \ube60\ub974\uba70 \uc77c\ubc18\uc801\uc73c\ub85c \uba87 \ubd84 \ubc16\uc5d0 \uac78\ub9ac\uc9c0 \uc54a\uc2b5\ub2c8\ub2e4.
\n\ud6a8\uc728 : \ud544\ub7ec \uc785\uc790\ub294 \ub9e4\uc6b0 \ubbf8\uc138\ud558\uace0 \uaddc\uce59\uc801\uc774\uba70 \uace0\uc815 \uc0c1\uc758 \ucf54\ud305\uc740 \uade0\uc77c\ud558\uace0 \uc9c8\ub7c9 \uc804\ub2ec \uc800\ud56d\uc740 \uc791\uae30 \ub54c\ubb38\uc5d0 \uceec\ub7fc \ud6a8\uc728\uc774 \ub9e4\uc6b0 \ub192\uc2b5\ub2c8\ub2e4.
\n\ub192\uc740 \uac10\ub3c4 : \uac80\ucd9c\uae30\ub294 \ub9e4\uc6b0 \ubbfc\uac10\ud569\ub2c8\ub2e4 : UV-10-9G, \ud615\uad11 \uac80\ucd9c\uae30 -10-11g.<\/p>\n
GC\uc640\uc758 \ube44\uad50 HPLC<\/p>\n
\uac1d\uccb4 \ubc0f \ubd84\uc11d \ubc94\uc704 : GC \ubd84\uc11d\uc740 \uac00\uc2a4 \ubc0f \ub0ae\uc740 \ubcf4\uc77c\ub9c1 \uc548\uc815 \ud654\ud569\ubb3c\ub85c \uc81c\ud55c\ub418\uba70, \uc774\ub294 \ucd1d \uc720\uae30\ubb3c\uc758 20%\uc5d0 \ubd88\uacfc\ud569\ub2c8\ub2e4.<\/p>\n
\uc774\ub3d9 \ub2e8\uacc4 \uc120\ud0dd : GC\ub294 \ubaa8\ubc14\uc77c \uc0c1\uc73c\ub85c \uc81c\ud55c\ub41c \uc120\ud0dd\uc801\uc778 \uc885\ub958\uc758 \"\ube44\ud65c\uc131\"\uac00\uc2a4\ub97c \uc0ac\uc6a9\ud558\uba70 \uad6c\uc131 \uc694\uc18c\uc5d0 \uc791\uc740 \uc601\ud5a5\uc744 \ubbf8\uce69\ub2c8\ub2e4.<\/p>\n
\uc791\ub3d9 \uc628\ub3c4 : GC\uc5d0\ub294 \uace0\uc628\uc774 \ud544\uc694\ud569\ub2c8\ub2e4.
\n\uc751\uc6a9 \ud504\ub85c\uadf8\ub7a8 \ud544\ub4dc
\n\ub192\uc740 \uac10\ub3c4, \uc815\ub7c9\uc801 \uc815\ud655\ub3c4 \ubc0f \ube44 \ud718\ubc1c\uc131 \ubc0f \uc5f4\uc801\uc73c\ub85c \ubd88\uc548\uc815\ud55c \uc131\ubd84\uc758 \ubd84\uc11d\uc5d0 \uc801\ud569\ud558\uae30 \ub54c\ubb38\uc5d0 HPLC \ubd84\ub9ac \ubd84\uc11d\uc740 \uc0b0\uc5c5 \ubc0f \uacfc\ud559\uc801 \uc5f0\uad6c, \ud2b9\ud788 \uc0dd\ubb3c\ud559 \ubc0f \uc758\ud559\uc5d0\uc11c \ub9e4\uc6b0 \ub2e4\uc591\ud569\ub2c8\ub2e4.<\/p>\n
HPLC\ub294 \ud38c\ud504 \uc2dc\uc2a4\ud15c, \uc8fc\uc785 \uc2dc\uc2a4\ud15c, \ubd84\ub9ac \uc2dc\uc2a4\ud15c, \ud0d0\uc9c0\uae30 \ubc0f \ub370\uc774\ud130 \ucc98\ub9ac \uc2dc\uc2a4\ud15c\uc73c\ub85c \uad6c\uc131\ub429\ub2c8\ub2e4.<\/p>\n
\uace0\uc555 \uc8fc\uc785 \uc2dc\uc2a4\ud15c
\n1) \uc774\ub3d9\uc131 \ubcd1 : 1-2L \uc720\ub9ac \ubcd1 1-2L \uc720\ub9ac \ubcd1 (NI \ud569\uae08), \uae30\uacf5\uc740 \ub300\ub7b5 2 \u03bcm\uc774\uba70 \ubbf8\ub9bd\uc790\uac00 \ud38c\ud551\ub418\ub294 \uac83\uc744 \ubc29\uc9c0\ud569\ub2c8\ub2e4.
\n2) Degasser : \ucd08\uc74c\ud30c \ud0c8\uae30 \ub610\ub294 \uc9c4\uacf5 \uac00\uc5f4 Degassing.<\/p>\n
3) \uace0\uc555 \ud38c\ud504 : \uc8fc\uc785 \ud38c\ud504\uc758 \uc694\uad6c \uc0ac\ud56d : \uc6b0\uc218\ud55c \ubc00\ubd09, \uc720\uc785\uc774\uc5c6\ub294 \uc548\uc815\uc801\uc778 \ud750\ub984, \ub113\uc740 \uc870\uc808 \uac00\ub2a5\ud55c \ubc94\uc704 \ubc0f \ubd80\uc2dd \uc800\ud56d.<\/p>\n
\uc2e4\ud5d8<\/p>\n
\uc2e4\ud5d8 \uc870\uac74
\n1) \uba54\ud0c4\uc62c\uc758 \ubaa8\ubc14\uc77c \ub2e8\uacc4 : \ubb3c = 90:10, 80:20 \ubc0f 70:30\uc758 \uc0c1\uc774\ud55c \ube44\uc728\ub85c 10\u03bcl \ud63c\ud569 \ud45c\uc900 \uc6a9\uc561 (\uba54\ud2f8 \ud30c\ub77c\ubca4, \ud504\ub85c\ud544 \ud30c\ub77c\ubca4 \ubc0f \ubd80\ud2f8 \ud30c\ub77c\ubca4)\uc744 \uc8fc\uc785\ud558\uc5ec \ub2e4\uc591\ud55c \uad6c\uc131 \uc694\uc18c\uc640 1.5\ubcf4\ub2e4 \ud070 \uc218\uc815 \uc2dc\uac04\uacfc \ucd5c\uc0c1\uc758 \ubcf4\uc720 \uc2dc\uac04\uc744 \uc120\ud0dd\ud558\uae30 \uc704\ud574 \ub2e4\uc591\ud55c \uad6c\uc131 \uc694\uc18c \uac04\uc758 \ud574\uc0c1\ub3c4\ub97c \ud14c\uc2a4\ud2b8\ud558\uae30 \uc704\ud574 \uba54\ud0c4\uc62c\uc758 \uc0c1\uc774\ud55c \ube44\uc728 : \ubb3c = 90:10, 80:20 \ubc0f 70:30\uc744 \uc8fc\uc785\ud55c\ub2e4.
\n\ucc38\uace0 : \uc774\ub3d9 \ub2e8\uacc4\ub97c \ubcc0\uacbd\ud560 \ub54c\ub9c8\ub2e4 \ud06c\ub85c\ub9c8\ud1a0 \uadf8\ub798\ud53c \uc2dc\uc2a4\ud15c\uc758 \uade0\ud615\uc744 \uc7a1\uae30 \uc704\ud574 \uc57d 5 \ubd84 \ub3d9\uc548 \uae30\ub2e4\ub824\uc57c\ud569\ub2c8\ub2e4 (\uc2e0\ud638 \ubc0f \uc555\ub825 \ub77c\uc778\uc774 \ubaa8\ub450 \uade0\ud615\uc744 \uc774\ub8f9\ub2c8\ub2e4).
\n2) 0.8, 1.0 \ubc0f 1.5 ml\uc758 \ub2e4\ub978 \uc774\ub3d9 \uc0c1 \uc720\ub7c9\uc5d0\uc11c 10 \u03bcl \ud63c\ud569 \ud45c\uc900 \uc6a9\uc561 (\uba54\ud2f8 \ud30c\ub77c\ubca4, \ud504\ub85c\ud544 \ud30c\ub77c\ubca4 \ubc0f \ubd80\ud2f8 \ud30c\ub77c\ubca4)\uc744 \uc8fc\uc785\ud558\uc5ec \ucd5c\uc801\uc758 \uc774\ub3d9 \uc0c1 \ud750\ub984 \uc18d\ub3c4\ub97c \uc120\ud0dd\ud55c \ucd5c\uc801\uc758 \uc774\ub3d9\uc131 \uc704\uc0c1 \ube44\uc728 \uc8fc\uc785 \ubd84\uc11d.<\/p>\n
\uc9c8\uc801 \ubd84\uc11d
\n\ucd5c\uc801\uc758 \uc2e4\ud5d8 \uc870\uac74 \ud558\uc5d0\uc11c, \uac01\uac01\uc758 \uba54\ud2f8 \ud30c\ub77c\ubca4, \ud504\ub85c\ud544 \ud30c\ub77c\ubca4 \ubc0f \ubd80\ud2f8 \ud30c\ub77c\ubca4 \uac01\uac01 10 \u03bcl\ub97c \uc8fc\uc785\ud558\uace0 \uac01 \ud53c\ud06c\uc758 \uccb4\ub958 \uc2dc\uac04\uc774 \uae30\ub85d\ub41c\ub2e4.<\/p>\n
\uc815\ub7c9 \ubd84\uc11d
\n\uad50\uc815 \uace1\uc120
\n\ucd5c\uc801\uc758 \uc2e4\ud5d8 \uc870\uac74 \ud558\uc5d0\uc11c, \ud63c\ud569 \ud45c\uc900 \uc0d8\ud50c\uc758 2, 5, 8, 10, 15 \ubc0f 20 \u03bcl\ub97c \uc8fc\uc0ac \ubc0f \ubd84\uc11d\ud558\uace0, \ud45c\uc900 \uace1\uc120\uc740 \uc8fc\uc785 \ubd80\ud53c \ubc0f \ud53c\ud06c \uba74\uc801\uc5d0 \uc758\ud574 \ud655\ub9bd\ub41c\ub2e4.
\n\uc774 \uace1\uc120\uc758 \uc0c1\uad00 \uacc4\uc218\ub294 0.99\ubcf4\ub2e4 \ud06c\uae30\uac00 \ud544\uc694\ud569\ub2c8\ub2e4.<\/p>\n
\uc54c \uc218\uc5c6\ub294 \uc0d8\ud50c \ubd84\uc11d
\n10 \u03bcl \ubbf8\uc9c0\uc758 \uc0d8\ud50c\uc744 \ud14c\uc2a4\ud2b8\ud558\uc5ec \uad50\uc815 \uace1\uc120\uc5d0 \uc758\ud55c \uc815\ub7c9 \ubd84\uc11d\uc744 \ud14c\uc2a4\ud2b8\ud569\ub2c8\ub2e4.<\/p>\n
\uc9c8\ubb38
\n\uc2e4\ud5d8 \ub370\uc774\ud130\uc5d0 \uae30\ucd08\ud558\uc5ec, \uac00\uc2a4 \ud06c\ub85c\ub9c8\ud1a0 \uadf8\ub798\ud53c\uc640 \uc561\uccb4 \ud06c\ub85c\ub9c8\ud1a0 \uadf8\ub798\ud53c \uc0ac\uc774\uc758 \ubd84\uc11d \ubc29\ubc95\uc758 \ucc28\uc774\ub97c \ub17c\uc758\ud558\uc2ed\uc2dc\uc624.
\nHPLC \ubd84\uc11d\uc758 \uc801\uc6a9 \uac12\uc744 \ud0d0\uc0c9\ud558\uc2ed\uc2dc\uc624.
\nUV \uac80\ucd9c\uae30\uac00 \ubaa8\ub4e0 \uc720\uae30 \ud654\ud569\ubb3c\uc744 \uac80\ucd9c\ud558\ub294 \ub370 \uc801\ud569\ud55c \uc9c0 \uc5ec\ubd80?<\/p>","protected":false},"excerpt":{"rendered":"
Experiment Objectives By experiments, learn the basic principles of chromatographic analysis and the basic operation of the instrument, and understand the process of data analysis. Learn about chromatographic quantitative methods and compare the advantages and disadvantages of different methods. HPLC Introduction High speed: HPLC uses high-pressure infusion equipment, the flow rate is greatly increased, and […]<\/p>\n","protected":false},"author":1,"featured_media":3315,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[76],"tags":[],"class_list":["post-2720","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-education"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.pgeneral.com\/ko\/wp-json\/wp\/v2\/posts\/2720","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.pgeneral.com\/ko\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.pgeneral.com\/ko\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/ko\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/ko\/wp-json\/wp\/v2\/comments?post=2720"}],"version-history":[{"count":0,"href":"https:\/\/www.pgeneral.com\/ko\/wp-json\/wp\/v2\/posts\/2720\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/ko\/wp-json\/wp\/v2\/media\/3315"}],"wp:attachment":[{"href":"https:\/\/www.pgeneral.com\/ko\/wp-json\/wp\/v2\/media?parent=2720"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.pgeneral.com\/ko\/wp-json\/wp\/v2\/categories?post=2720"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.pgeneral.com\/ko\/wp-json\/wp\/v2\/tags?post=2720"}],"curies":[{"name":"WP","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}