{"id":3729,"date":"2025-07-15T14:28:11","date_gmt":"2025-07-15T06:28:11","guid":{"rendered":"https:\/\/www.pgeneral.com\/?p=3729"},"modified":"2025-07-15T14:28:11","modified_gmt":"2025-07-15T06:28:11","slug":"determination-of-palladium-in-oseltamivir-phosphate-by-graphite-furnace-atomic-absorption-spectrometry","status":"publish","type":"post","link":"https:\/\/www.pgeneral.com\/ja\/applications\/determination-of-palladium-in-oseltamivir-phosphate-by-graphite-furnace-atomic-absorption-spectrometry\/","title":{"rendered":"\u30b0\u30e9\u30d5\u30a3\u30c3\u30c8\u7089\u539f\u5b50\u5438\u53ce\u5206\u5149\u8a08\u306b\u3088\u308b\u30aa\u30bb\u30eb\u30bf\u30df\u30d3\u30eb\u30ea\u30f3\u9178\u4e2d\u306e\u30d1\u30e9\u30c7\u30a3\u30a6\u30e0\u306e\u6c7a\u5b9a"},"content":{"rendered":"
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  1. Method Overview<\/li>\n<\/ol>\n

    After the sample is dissolved in 2% nitric acid, it is injected into the graphite furnace of an atomic absorption spectrophotometer. After electrothermal atomization, it absorbs the resonance line at 247.6nm. Within a certain concentration range, the absorbance is proportional to the palladium content, and quantification is performed by comparison with the standard series.<\/p>\n

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    1. 2. Instruments and Reagents<\/li>\n<\/ol>\n

      2.1 Instruments and Equipment<\/p>\n

      2.1.1 Detection Instruments<\/p>\n

      Graphite furnace atomic absorption spectrophotometer (Palladium\u00a0hollow cathode lamp)<\/p>\n

      Argon gas 1 cylinder Purity \u2265 99.99%<\/p>\n

      Cooling circulating water system \u00a0(Rated water pump flow: 3.5L\/min)<\/p>\n

      2.1.2 Pretreatment Equipment<\/p>\n

      Electronic balance<\/p>\n

      Ultrapure water system<\/p>\n

      Micropipette<\/p>\n

      Volumetric flask<\/p>\n

      Test tube Several<\/p>\n

      2.2 Reagents<\/p>\n

      2.2.1 Reagents<\/p>\n

      Nitric acid<\/p>\n

      2.2.2 Prepared Reagents<\/p>\n

      (2+98) Nitric acid solution Measure 20mL of nitric acid, slowly add it to 980mL of water, and mix well.<\/p>\n

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      1. 3. Operation Process<\/li>\n<\/ol>\n

        3.1 Sample Treatment<\/p>\n

        3.1.1 Preparation of Test Solution<\/p>\n

        Accurately weigh 0.04g of the sample into a 10mL test tube, dilute to the mark with (2+98) nitric acid solution, mix well, and set aside as the sample test solution. Prepare a reagent blank solution simultaneously.<\/p>\n

        3.1.2 Preparation of Standard Solutions<\/p>\n

        1) Preparation of palladium standard intermediate solution:<\/p>\n

        Palladium standard solution (1.0\u03bcg\/mL): Accurately pipette 0.1mL of the palladium standard solution (1000\u03bcg\/mL) into a 100mL volumetric flask, dilute to the mark with (2+98) nitric acid solution, and shake well.<\/p>\n

          \n
        • Preparation of palladium standard series:<\/li>\n<\/ul>\n

          Accurately pipette 0.0, 0.1, 0.2, 0.3, and 0.4 mL of the palladium standard solution (1.0 \u03bcg\/mL) into 10mL volumetric flasks respectively, dilute to the mark with (2+98) nitric acid solution to obtain palladium standard series solutions with concentrations of 0.0, 10.0, 20.0, 30.0, 40.0 ng\/mL.<\/p>\n

          3.2 Sample Testing<\/p>\n

          1) Testing Conditions<\/p>\n

          Reference conditions for graphite furnace atomic absorption spectrophotometer detection<\/p>\n

          Element Palladium<\/p>\n

          Wavelength (nm) 247.6<\/p>\n

          Spectral bandwidth (nm) 0.2<\/p>\n

          Element lamp current (mA) 6<\/p>\n

          Background correction method Deuterium lamp<\/p>\n

          2) Sample Testing
          \nInject 15\u03bcL of the palladium standard series solutions into the graphite furnace in order of increasing mass concentration. After atomization, measure the absorbance. Plot a standard curve with mass concentration as the abscissa and absorbance as the ordinate. Read the data, input the sample weight, and check and record the results.<\/p>\n

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          1. Result Calculation<\/li>\n<\/ol>\n

            \"\"<\/p>\n

            Where:<\/p>\n

            X\u00a0— Palladium content in the sample (mg\/kg);<\/p>\n

            \u03c1– Mass concentration of palladium in the sample test solution(\u03bcg\/L);<\/p>\n

            \u03c10\u00a0— Mass concentration of palladium in the blank solution(\u03bcg\/L);<\/p>\n

            V\u00a0— Volume of the sample digestion solution after constant volume(mL);<\/p>\n

            m\u00a0— Mass of the sample (g);<\/p>\n

            1000 — Conversion factor.
            \n