![\"\u30b5\u30a4\u30ba\u9664\u5916\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\"](\"http:\/\/www.pgeneral.com\/wp-content\/uploads\/2025\/04\/1-size-exclusion-chromatography.webp\")
<\/p>\n<\/p>\n
\u30e9\u30dc\u3067\u5206\u5b50\u3092\u5206\u6790\u3059\u308b\u5834\u5408\u3001\u4fe1\u983c\u3067\u304d\u308b\u7d50\u679c\u3092\u5f97\u308b\u306b\u306f\u3001\u9069\u5207\u306a\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u65b9\u6cd5\u3092\u9078\u629e\u3059\u308b\u3053\u3068\u304c\u91cd\u8981\u3067\u3059\u3002<\/p>\n
SEC\u304a\u3088\u3073HPLC\u306e\u8a73\u7d30\u3092\u8abf\u67fb\u3059\u308b\u524d\u306b\u3001\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u306e\u30b3\u30a2\u30b3\u30f3\u30bb\u30d7\u30c8\u3092\u7406\u89e3\u3059\u308b\u3053\u3068\u306f\u4fbf\u5229\u3067\u3059\u3002<\/p>\n
Gel\u308d\u904e\u307e\u305f\u306f\u30b2\u30eb\u900f\u904e\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u3068\u3057\u3066\u3082\u77e5\u3089\u308c\u308bSEC\u306f\u3001\u30b5\u30a4\u30ba\u3054\u3068\u306b\u5206\u5b50\u3092\u5206\u96e2\u3059\u308b\u305f\u3081\u306b\u4f7f\u7528\u3059\u308b\u65b9\u6cd5\u3067\u3059\u3002<\/p>\n
HPLC\u306f\u3001\u6975\u6027\u3084\u96fb\u8377\u306a\u3069\u306e\u7279\u6027\u306b\u57fa\u3065\u3044\u3066\u5225\u3005\u306e\u5316\u5408\u7269\u306b\u9069\u7528\u3059\u308b\u67d4\u8edf\u306a\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u6280\u8853\u3067\u3059\u3002<\/p>\n
SEC\u3068HPLC\u304c\u3069\u306e\u3088\u3046\u306b\u7570\u306a\u308b\u304b\u3092\u628a\u63e1\u3059\u308b\u306b\u306f\u3001\u305d\u308c\u3089\u306e\u57fa\u672c\u7684\u306a\u30e1\u30ab\u30cb\u30ba\u30e0\u3092\u8abf\u3079\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n
SEC\u3067\u306f\u3001\u591a\u5b54\u8cea\u306e\u56fa\u5b9a\u76f8\u3092\u4f7f\u7528\u3057\u307e\u3059\u3002<\/p>\n
HPLC\u306f\u3001\u5316\u5b66\u7684\u76f8\u4e92\u4f5c\u7528\u3068\u5b9a\u5e38\u671f\u3068\u306e\u5206\u5b50\u3092\u5206\u96e2\u3057\u307e\u3059\u3002<\/p>\n
SEC\u3068HPLC\u306e\u65b9\u6cd5\u8ad6\u306f\u3001\u30bf\u30b9\u30af\u306e\u7cbe\u5ea6\u3068\u9069\u5408\u6027\u3092\u5b9a\u7fa9\u3057\u307e\u3059\u3002<\/p>\n
Sec\u3067\u306f\u3001\u901a\u5e38\u3001\u30a2\u30ac\u30ed\u30fc\u30b9\u3084\u30b7\u30ea\u30ab\u306a\u3069\u306e\u591a\u5b54\u8cea\u30d3\u30fc\u30ba\u306e\u56fa\u5b9a\u76f8\u3092\u4f7f\u7528\u3057\u307e\u3059\u3002<\/p>\n
HPLC\u306f\u3055\u307e\u3056\u307e\u306a\u5b9a\u5e38\u6bb5\u968e\u3092\u63d0\u4f9b\u3057\u307e\u3059\u3002<\/p>\n
SEC\u3068HPLC\u306e\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306f\u3001\u7cbe\u5bc6\u5206\u6790\u306b\u304a\u3051\u308b\u305d\u306e\u5f37\u307f\u3092\u5f37\u8abf\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n
SEC\u306f\u3001\u5927\u304d\u306a\u751f\u4f53\u5206\u5b50\u3092\u5206\u6790\u3059\u308b\u305f\u3081\u306e\u597d\u307e\u3057\u3044\u65b9\u6cd5\u3067\u3059\u3002<\/p>\n
HPLC\u306f\u3001\u85ac\u7269\u958b\u767a\u304b\u3089\u98df\u54c1\u306e\u5b89\u5168\u6027\u307e\u3067\u306e\u7528\u9014\u306b\u512a\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n
\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u306e\u7cbe\u5ea6\u306f\u3001\u89e3\u50cf\u5ea6\u3084\u611f\u5ea6\u306a\u3069\u306e\u8981\u56e0\u306b\u4f9d\u5b58\u3057\u307e\u3059\u3002<\/p>\n
SEC\u306f\u3001\u30b5\u30a4\u30ba\u3054\u3068\u306b\u5206\u5b50\u3092\u5206\u96e2\u3059\u308b\u305f\u3081\u306e\u56fa\u4f53\u89e3\u50cf\u5ea6\u3092\u63d0\u4f9b\u3057\u307e\u3059\u3002<\/p>\n
HPLC\u306f\u3001\u591a\u304f\u306e\u5834\u5408\u3001\u3088\u308a\u5927\u304d\u306a\u89e3\u50cf\u5ea6\u3068\u611f\u5ea6\u3092\u63d0\u4f9b\u3057\u307e\u3059\u3002<\/p>\n
\u30e9\u30dc\u3092\u88c5\u5099\u3059\u308b\u3068\u304d\u3001\u6a5f\u5668\u3084\u30e1\u30f3\u30c6\u30ca\u30f3\u30b9\u306a\u3069\u306e\u5b9f\u7528\u7684\u306a\u8981\u56e0\u304c\u3042\u306a\u305f\u306e\u9078\u629e\u306e\u30c6\u30af\u30cb\u30c3\u30af\u306b\u5f71\u97ff\u3057\u307e\u3059\u3002<\/p>\n
SEC\u306e\u5834\u5408\u3001\u591a\u5b54\u8cea\u30d3\u30fc\u30ba\u306e\u5217\u3092\u5099\u3048\u305f\u30b7\u30b9\u30c6\u30e0\u304c\u5fc5\u8981\u3067\u3059\u3002<\/p>\n
HPLC\u30b7\u30b9\u30c6\u30e0\u306f\u3088\u308a\u8907\u96d1\u3067\u3059\u3002<\/p>\n
\u5404\u624b\u6cd5\u306b\u306f\u9577\u6240\u3068\u77ed\u6240\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n
SEC\u306f\u3001\u904e\u9177\u306a\u6761\u4ef6\u306a\u3057\u3067\u5927\u304d\u306a\u5206\u5b50\u3092\u5206\u6790\u3059\u308b\u3053\u3068\u306b\u512a\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n
HPLC\u306e\u5f37\u3055\u306f\u3001\u305d\u306e\u67d4\u8edf\u6027\u3068\u9ad8\u611f\u5ea6\u3067\u3059\u3002<\/p>\n
SEC\u306f\u30b5\u30a4\u30ba\u30d9\u30fc\u30b9\u306e\u5206\u96e2\u306b\u7126\u70b9\u3092\u5f53\u3066\u3066\u3044\u307e\u3059\u3002<\/p>\n
HPLC\u306f\u6700\u9069\u5316\u3059\u308b\u306e\u304c\u96e3\u3057\u3044\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n
SEC\u3068HPLC\u3092\u9078\u629e\u3059\u308b\u3053\u3068\u306f\u3001\u5206\u6790\u76ee\u6a19\u306b\u3088\u3063\u3066\u7570\u306a\u308a\u307e\u3059\u3002<\/p>\n
\u5927\u304d\u306a\u751f\u4f53\u5206\u5b50\u3092\u4f7f\u7528\u3059\u308b\u3068\u304d\u306bSEC\u3092\u9078\u629e\u3057\u307e\u3059\u3002<\/p>\n
\u5c0f\u5206\u5b50\u3092\u5206\u6790\u3057\u305f\u308a\u3001\u4e0d\u7d14\u7269\u3092\u5b9a\u91cf\u5316\u3059\u308b\u305f\u3081\u306bHPLC\u3092\u9078\u629e\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n
![\"\u5fcd\u8010\"](\"http:\/\/www.pgeneral.com\/wp-content\/uploads\/2025\/04\/PERSEE-1.webp\")
<\/p>\n
\u30e9\u30dc\u3092\u30bb\u30c3\u30c8\u30a2\u30c3\u30d7\u3059\u308b\u3068\u304d \u79d2\u3001<\/u><\/a>\u00a0\u4fe1\u983c\u3067\u304d\u308b\u30b5\u30d7\u30e9\u30a4\u30e4\u30fc\u3092\u9078\u629e\u3059\u308b\u3053\u3068\u304c\u4e0d\u53ef\u6b20\u3067\u3059\u3002 \u5fcd\u8010<\/u><\/a>\u00a0\u306e\u4fe1\u983c\u3067\u304d\u308b\u30d7\u30ed\u30d0\u30a4\u30c0\u30fc\u3067\u3059 \u5206\u6790\u6a5f\u5668<\/u><\/a>\u3002 \u9811\u4e08\u306a\u30b7\u30b9\u30c6\u30e0<\/u><\/a>\u3002<\/p>\n SEC\u3092\u5927\u304d\u306a\u751f\u4f53\u5206\u5b50\u306b\u4f7f\u7528\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n \u306f\u3044\u3001\u751f\u4f53\u5206\u5b50\u5206\u6790\u306b\u306fHPLC\u3092\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002<\/p>\n \u30b5\u30f3\u30d7\u30eb\u306e\u7a2e\u985e\u3068\u5206\u6790\u76ee\u6a19\u3092\u8003\u616e\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n <\/p>","protected":false},"excerpt":{"rendered":" When analyzing molecules in a lab, selecting the appropriate chromatography method is crucial for obtaining reliable results. Size exclusion chromatography (SEC) and high-performance liquid chromatography (HPLC) are two commonly used techniques. Each has distinct advantages. Knowing their differences helps you choose the method that suits your analytical objectives. This blog examines how SEC and HPLC vary in precision analysis. It explores their mechanisms, applications, and practical aspects. Understanding Chromatography Basics Before exploring SEC and HPLC specifics, it\u2019s useful to understand chromatography\u2019s core concepts. These methods separate molecules based on their interactions with a stationary phase and a mobile phase. Let\u2019s define each technique to prepare for their comparison. Defining Size […]<\/p>\n","protected":false},"author":1,"featured_media":3511,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[38,37],"tags":[],"class_list":["post-3510","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-industry-information","category-news"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.pgeneral.com\/ja\/wp-json\/wp\/v2\/posts\/3510","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.pgeneral.com\/ja\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.pgeneral.com\/ja\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/ja\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/ja\/wp-json\/wp\/v2\/comments?post=3510"}],"version-history":[{"count":0,"href":"https:\/\/www.pgeneral.com\/ja\/wp-json\/wp\/v2\/posts\/3510\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/ja\/wp-json\/wp\/v2\/media\/3511"}],"wp:attachment":[{"href":"https:\/\/www.pgeneral.com\/ja\/wp-json\/wp\/v2\/media?parent=3510"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.pgeneral.com\/ja\/wp-json\/wp\/v2\/categories?post=3510"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.pgeneral.com\/ja\/wp-json\/wp\/v2\/tags?post=3510"}],"curies":[{"name":"WP","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}\u30b5\u30a4\u30ba\u306e\u9664\u5916\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u3068HPLC\u306b\u95a2\u3059\u308bFAQ<\/h2>\n
\u30b5\u30a4\u30ba\u306e\u9664\u5916\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u306b\u6700\u9069\u306a\u30b5\u30f3\u30d7\u30eb\u306e\u7a2e\u985e\u306f\u4f55\u3067\u3059\u304b\uff1f<\/h3>\n
HPLC\u306f\u3001SEC\u306e\u3088\u3046\u306a\u751f\u4f53\u5206\u5b50\u5206\u6790\u306b\u4f7f\u7528\u3067\u304d\u307e\u3059\u304b\uff1f<\/h3>\n
\u30e9\u30dc\u306eSEC\u3068HPLC\u3092\u9078\u629e\u3059\u308b\u306b\u306f\u3069\u3046\u3059\u308c\u3070\u3088\u3044\u3067\u3059\u304b\uff1f<\/h3>\n