{"id":2799,"date":"2023-09-14T13:29:59","date_gmt":"2023-09-14T05:29:59","guid":{"rendered":"http:\/\/www.pgeneral.com\/?p=2799"},"modified":"2023-09-14T13:30:06","modified_gmt":"2023-09-14T05:30:06","slug":"%e7%b4%ab%e5%a4%96%e5%8f%af%e8%a6%96%e5%88%86%e5%85%89%e5%85%89%e5%ba%a6%e8%a8%88%e3%81%ab%e3%82%88%e3%82%8b%e8%9c%82%e8%9c%9c%e4%b8%ad%e3%81%ae%e3%82%b8%e3%82%a2%e3%82%b9%e3%82%bf%e3%83%bc%e3%82%bc","status":"publish","type":"post","link":"https:\/\/www.pgeneral.com\/ja\/applications\/food-and-drugs\/method-for-the-determination-of-diastase-number-in-honey-by-uv-vis-spectrophotometer","title":{"rendered":"UV-vis\u5206\u5149\u5149\u5ea6\u8a08\u306b\u3088\u308b\u8702\u871c\u4e2d\u306e\u30b8\u30a2\u30b9\u30bf\u30fc\u30bc\u6570\u306e\u6e2c\u5b9a\u65b9\u6cd5"},"content":{"rendered":"

\u539f\u7406
\n\u30c7\u30f3\u30d7\u30f3\u6eb6\u6db2\u3092\u8702\u871c\u306e\u30b5\u30f3\u30d7\u30eb\u6eb6\u6db2\u306b\u5165\u308c\u305f\u5f8c\u3001\u3044\u304f\u3064\u304b\u306e\u6fb1\u7c89\u306f\u8702\u871c\u4e2d\u306e\u30b8\u30a2\u30b9\u30bf\u30bc\u306b\u3088\u3063\u3066\u52a0\u6c34\u5206\u89e3\u3055\u308c\u307e\u3059\u3002<\/p>\n

\u8a66\u85ac\u3068\u6750\u6599
\n\u7279\u5b9a\u306e\u901a\u77e5\u304c\u3042\u308b\u4eba\u3092\u9664\u304d\u3001AR\u5ea6\u8a66\u85ac\u304c\u4f7f\u7528\u3055\u308c\u307e\u3059\u3002<\/p>\n

\u8a66\u85ac\uff1a
\n1\u3002\u30e8\u30a6\u7d20\u3002
\n2\u3002\u30e8\u30a6\u5316\u30ab\u30ea\u30a6\u30e0\u3002
\n3\u3002\u9162\u9178\u30ca\u30c8\u30ea\u30a6\u30e0\uff08CH3COONA\u30fb3H2O\uff09\u3002
\n4\u3002\u30a2\u30a4\u30b9\u30a2\u30bb\u30c6\u30fc\u30c8\u9178
\n5\u3002\u5869\u5316\u30ca\u30c8\u30ea\u30a6\u30e0\u3002
\n6\u3002\u53ef\u6eb6\u6027\u6fb1\u7c89\u3002
\n7\u3002\u30e8\u30a6\u7d20\u30b9\u30c8\u30c3\u30af\u6eb6\u6db2\uff1a\u4f53\u91cd8.8g\u306e\u30e8\u30a6\u7d20\u3068\u30e8\u30a6\u5316\u30ab\u30ea\u30a6\u30e022g\u300130ml\u301c40ml\u306e\u6c34\u3068\u6df7\u5408\u3057\u30011000ml\u306b\u5e0c\u91c8\u3057\u307e\u3059\u3002
\n8\u3002\u30e8\u30a6\u7d20\u6eb6\u6db2\uff1a\u4f53\u91cd20g\u306e\u30e8\u30a6\u7d20\u30ab\u30ea\u30a6\u30e0\u30015mL\u306e\u30e8\u30a6\u7d20\u30b9\u30c8\u30c3\u30af\u6eb6\u6db2\u3092\u52a0\u3048\u3001\u6c34\u3067500ml\u306b\u5e0c\u91c8\u3057\u307e\u3059\uff08\u3053\u306e\u6eb6\u6db2\u306f2\u65e5\u9593\u3057\u304b\u4f7f\u7528\u3067\u304d\u307e\u305b\u3093\uff09\u3002
\n9. \u9162\u9178\u7de9\u885d\u6db2\uff1apH 5.3\uff081.59 mol\/L\uff09\u3002\u9162\u9178\u30ca\u30c8\u30ea\u30a6\u30e087g\u3092\u91cf\u308a\u53d6\u308a\u3001\u6c34400mL\u3067\u5e0c\u91c8\u3059\u308b\u3002\u6c37\u51b7\u3057\u305f\u9162\u917810.5mL\u3092\u52a0\u3048\u3066500mL\u306b\u5e0c\u91c8\u3059\u308b\u3002\u5fc5\u8981\u306b\u5fdc\u3058\u3066\u3001\u9162\u9178\u30ca\u30c8\u30ea\u30a6\u30e0\u307e\u305f\u306f\u9162\u9178\u3092\u7528\u3044\u3066pH\u30925.3\u306b\u8abf\u6574\u3059\u308b\u3002
\n10.\u5869\u5316\u30ca\u30c8\u30ea\u30a6\u30e0\u6eb6\u6db2\uff080.5mol\/L\uff09\uff1aNaCl 14.5g\u3092\u91cf\u308a\u3001\u6c34500mL\u306b\u6eb6\u304b\u3057\u307e\u3059\u3002
\n11\u3002\u6fb1\u7c89\u6eb6\u6db2\uff1a90ml\u306e\u6c34\u306b2G\u53ef\u6eb6\u6027\u6fb1\u7c89\u3092\u6eb6\u89e3\u3057\u307e\u3059\u3002<\/p>\n

\u88c5\u7f6e\uff1a
\n1\u3002UV-VIS\u5206\u5149\u5149\u5ea6\u8a08\u3002
\n2\u3002\u4e00\u5b9a\u6e29\u5ea6\u30bb\u30eb\u30db\u30eb\u30c0\u30fc<\/p>\n

\u30b5\u30f3\u30d7\u30eb\u306e\u6e96\u5099\u3068\u4fdd\u5b58
\n\u30b5\u30f3\u30d7\u30eb\u306e\u6e96\u5099\uff1a
\n\u30b5\u30f3\u30d7\u30eb\u3092\u3088\u304f\u652a\u62cc\u3057\u3001\u30c6\u30b9\u30c8\u30b5\u30f3\u30d7\u30eb\u3068\u3057\u30660.5kg\u3092\u30b5\u30f3\u30d7\u30eb\u30dc\u30c8\u30eb\u306b\u79fb\u3057\u307e\u3059\u3002
\n\u6ce8\uff1a\u30af\u30ea\u30b9\u30bf\u30eb\u304c\u8868\u793a\u3055\u308c\u308b\u304b\u3069\u3046\u304b\u306b\u304b\u304b\u308f\u3089\u305a\u3001\u3059\u3079\u3066\u306e\u30b5\u30f3\u30d7\u30eb\u3092\u52a0\u71b1\u3059\u308b\u3053\u3068\u306f\u3067\u304d\u307e\u305b\u3093\u3002<\/p>\n

\u30b5\u30f3\u30d7\u30eb\u4fdd\u5b58\uff1a
\n\u30b5\u30f3\u30d7\u30eb\u306f\u5ba4\u6e29\u3067\u4fdd\u5b58\u3067\u304d\u307e\u3059\u3002<\/p>\n

\u5b9f\u9a13\u624b\u9806
\n\u6fb1\u7c89\u6eb6\u6db2\u306e\u6a19\u6e96\u30c6\u30b9\u30c8
\n5.0 mL\u306e\u6fb1\u7c89\u6eb6\u6db2\uff08\u8a66\u85ac11\uff09\u306810.0 mL\u306e\u6c34\u3092\u6e2c\u5b9a\u3057\u300140\u2103\u306e\u6c34\u6d74\u306b\u500b\u5225\u306b15\u5206\u9593\u914d\u7f6e\u3057\u307e\u3059\u3002<\/p>\n

\u30b5\u30f3\u30d7\u30eb\u30c6\u30b9\u30c8\uff1a
\n1\u3002\u5206\u5149\u5149\u5ea6\u8a08\u306e\u6761\u4ef6\uff1a
\na\uff09\u6ce2\u9577\uff1a660nm
\nb\uff09\u53c2\u7167\uff1a\u6c34
\n2\u3002\u30b5\u30f3\u30d7\u30eb\u524d\u51e6\u7406\uff1a5G\uff08\u00b10.01g\uff09\u8abf\u88fd\u3057\u305f\u30b5\u30f3\u30d7\u30eb\u3092\u6e2c\u5b9a\u3057\u300120ml\u30d3\u30fc\u30ab\u30fc\u306b\u79fb\u3057\u307e\u3059\u3002
\n3. 5ml\u306e\u6fb1\u7c89\u6eb6\u6db2\u300110ml\u30b5\u30f3\u30d7\u30eb\u6eb6\u6db2\uff08\u30b5\u30f3\u30d7\u30eb\u30c6\u30b9\u30c82\uff09\u3001\u304a\u3088\u307310ml\u306e\u30e8\u30a6\u7d20\u6eb6\u6db2\u3092\u6e2c\u5b9a\u3057\u300140\u500b\u306e\u6c34\u6d74\u306b\u500b\u5225\u306b15\u5206\u9593\u914d\u7f6e\u3057\u307e\u3059\u3002
\n4. 5\u5206\u5f8c\u30011.0ml\u306e\u6df7\u5408\u6eb6\u6db2\u309210ml\u52a0\u71b1\u30e8\u30a6\u7d20\u6eb6\u6db2\u306b\u79fb\u3057\u3001\u8a18\u9332\u3055\u308c\u305f\u91cf\u306e\u6c34\uff08\u6fb1\u7c89\u6eb6\u6db2\u30b9\u30c6\u30c3\u30d7\u306e\u6a19\u6e96\u30c6\u30b9\u30c8\u3067\u8a18\u9332\uff09\u3067\u5e0c\u91c8\u3057\u3001\u518d\u3073\u3088\u304f\u6df7\u5408\u3057\u307e\u3059\u3002
\n5.\u5438\u5149\u5ea6\u304c0.235\u3092\u8d85\u3048\u308b\u5834\u5408\u3001\u5438\u5149\u5ea6\u304c0.235\u672a\u6e80\u306b\u306a\u308b\u307e\u3067\u30014\u756a\u76ee\u306e\u30b9\u30c6\u30c3\u30d7\u3092\u7e70\u308a\u8fd4\u3059\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002
\n6.\u6e2c\u5b9a\u4e2d\u3001\u6df7\u5408\u6eb6\u6db2\u3001\u30e8\u30a6\u7d20\u6eb6\u6db2\u3001\u304a\u3088\u3073\u6c34\u309240\u306e\u6c34\u6d74\u3067\u7dad\u6301\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n

\u8a08\u7b97
\n\u5bfe\u6570\u5ea7\u6a19\u8ad6\u6587\u3067\u306f\u3001y\u8ef8\u3092\u5438\u5149\u5ea6\uff08\uff05\uff09\u3068\u547c\u3073\u3001x\u8ef8\u306f\u6642\u9593\uff08min\uff09\u3068\u3057\u3066\u540d\u524d\u3092\u4ed8\u3051\u307e\u3059\u3002<\/p>\n

\"\"<\/p>\n

\u3053\u3053\u3067\u3001x\u306f\u6eb6\u6db2\u4e2d\u306e\u30b8\u30a2\u30b9\u30bf\u30fc\u30bc\u6570\uff08ml\/\uff08g\u30fbh\uff09\uff09\u3001t\u306f\u5bfe\u5fdc\u3059\u308b\u6642\u9593\uff08min\uff09\u3067\u3059\u3002<\/p>\n

\u7d50\u679c
\n\u3053\u306e\u65b9\u6cd5\u3092\u4f7f\u7528\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u5b9f\u9a13\u306e\u518d\u73fe\u6027\u3068\u518d\u73fe\u6027\u306f95\uff05\u4ee5\u4e0a\u3067\u3059\u3002<\/p>","protected":false},"excerpt":{"rendered":"

Principle After putting starch solution into honey sample solution, some starch is hydrolysis by diastase in honey. The starch residue appears as bluish violet color after mixing with iodine. As the reaction process, the color disappears finally. By using UV-Vis Spectrophotometer at 660nm wavelength, the time required to reach the specific absorbance can be measured. Therefore, the volume (ml) that 1% starch sample is hydrolyzed by 1g of honey in 1h can be calculated. Reagents and Material Excepts those with specific notification, AR degree reagents are used; water is ultrapure water. Reagent: 1. Iodine. 2. Potassium Iodide. 3. Sodium Acetate (CH3COONa \u00b73H2O). 4. Iced Acetate Acid 5. Sodium Chloride. 6. […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[73],"tags":[],"class_list":["post-2799","post","type-post","status-publish","format-standard","hentry","category-food-and-drugs"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.pgeneral.com\/ja\/wp-json\/wp\/v2\/posts\/2799","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.pgeneral.com\/ja\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.pgeneral.com\/ja\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/ja\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/ja\/wp-json\/wp\/v2\/comments?post=2799"}],"version-history":[{"count":0,"href":"https:\/\/www.pgeneral.com\/ja\/wp-json\/wp\/v2\/posts\/2799\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.pgeneral.com\/ja\/wp-json\/wp\/v2\/media?parent=2799"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.pgeneral.com\/ja\/wp-json\/wp\/v2\/categories?post=2799"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.pgeneral.com\/ja\/wp-json\/wp\/v2\/tags?post=2799"}],"curies":[{"name":"WP","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}