{"id":2784,"date":"2023-09-14T12:23:24","date_gmt":"2023-09-14T04:23:24","guid":{"rendered":"http:\/\/www.pgeneral.com\/?p=2784"},"modified":"2026-01-22T11:47:44","modified_gmt":"2026-01-22T03:47:44","slug":"gcms%e3%81%ab%e3%82%88%e3%82%8b%e9%a3%9f%e5%93%81%e4%b8%ad%e3%81%ae%e6%ae%8b%e7%95%99%e3%82%af%e3%83%ad%e3%83%ab%e3%83%94%e3%83%aa%e3%83%9b%e3%82%b9%e3%81%ae%e5%ae%9a%e9%87%8f","status":"publish","type":"post","link":"https:\/\/www.pgeneral.com\/ja\/%e3%82%a2%e3%83%97%e3%83%aa%e3%82%b1%e3%83%bc%e3%82%b7%e3%83%a7%e3%83%b3\/%e9%a3%9f%e5%93%81%e3%81%a8%e5%8c%bb%e8%96%ac%e5%93%81\/gcms%e3%81%ab%e3%82%88%e3%82%8b%e9%a3%9f%e5%93%81%e4%b8%ad%e3%81%ae%e6%ae%8b%e7%95%99%e3%82%af%e3%83%ad%e3%83%ab%e3%83%94%e3%83%aa%e3%83%9b%e3%82%b9%e3%81%ae%e5%ae%9a%e9%87%8f\/","title":{"rendered":"GCMS\u306b\u3088\u308b\u98df\u54c1\u4e2d\u306e\u30af\u30ed\u30eb\u30d4\u30ea\u30db\u30b9\u6b8b\u57fa\u306e\u6c7a\u5b9a"},"content":{"rendered":"<p>1\u30b9\u30b3\u30fc\u30d7<br \/>\n\u3053\u306e\u6a19\u6e96\u306f\u3001\u30ac\u30b9\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc - \u708e\u6e2c\u5149\u691c\u51fa\u5668\uff08GC -FPD\uff09\u304a\u3088\u3073\u30ac\u30b9\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc - \u8cea\u91cf\u5206\u6790\uff08GC -MS\uff09\u306b\u3088\u308b\u98df\u54c1\u4e2d\u306e\u30af\u30ed\u30eb\u30d4\u30ea\u30db\u30b9\u6b8b\u57fa\u3092\u30c6\u30b9\u30c8\u3059\u308b\u65b9\u6cd5\u3092\u6307\u5b9a\u3057\u307e\u3059\u3002<br \/>\n\u3053\u306e\u57fa\u6e96\u306f\u3001\u30c8\u30a6\u30e2\u30ed\u30b3\u30b7\u3001\u8336\u8272\u306e\u30e9\u30a4\u30b9\u3001\u30cd\u30ae\u3001\u307b\u3046\u308c\u3093\u8349\u3001\u5510\u8f9b\u5b50\u3001\u30aa\u30ec\u30f3\u30b8\u3001\u30ea\u30f3\u30b4\u3001\u677e\u3001\u30cc\u30c3\u30c8\u30ab\u30fc\u30cd\u30eb\u3001\u8336\u3001\u8702\u871c\u3001\u9b5a\u3001\u8702\u871c\u3001\u8c5a\u8089\u3001\u9d8f\u306e\u814e\u81d3\u3001\u9d8f\u306e\u809d\u81d3\u306b\u304a\u3051\u308b\u30af\u30ed\u30eb\u30d4\u30ea\u30db\u30b9\u306e\u6b8b\u7559\u7269\u542b\u6709\u91cf\u306e\u6c7a\u5b9a\u3068\u78ba\u8a8d\u306b\u9069\u7528\u3067\u304d\u307e\u3059\u3002<\/p>\n<p>2\u3064\u306e\u539f\u5247<\/p>\n<p>\u30aa\u30ec\u30f3\u30b8\u307e\u305f\u306f\u4ed6\u306e\u690d\u7269\u30b5\u30f3\u30d7\u30eb\u306f\u3001\u9162\u9178\u30a8\u30c1\u30eb\u3067\u62bd\u51fa\u3055\u308c\u307e\u3059\u3002<\/p>\n<p>3\u3064\u306e\u8a66\u85ac\u3068\u6750\u6599<\/p>\n<p>\u4f7f\u7528\u3055\u308c\u308b\u3059\u3079\u3066\u306e\u8a66\u85ac\u306f\u3001\u7279\u306b\u6307\u5b9a\u304c\u306a\u3044\u9650\u308a\u3001\u5206\u6790\u7684\u306b\u7d14\u7c8b\u3067\u306a\u3051\u308c\u3070\u306a\u308a\u307e\u305b\u3093\u3002<br \/>\n3.1\u30a2\u30bb\u30c8\u30f3\uff1a\u6b8b\u7559\u30b0\u30ec\u30fc\u30c9\u3002<\/p>\n<p>3.2\u30b8\u30af\u30ed\u30ed\u30e1\u30bf\u30f3\uff1a\u6b8b\u57fa\u30b0\u30ec\u30fc\u30c9\u3002<\/p>\n<p>3.3\u30b7\u30af\u30ed\u30d8\u30ad\u30b5\u30f3\uff1a\u6b8b\u7559\u30b0\u30ec\u30fc\u30c9<br \/>\n3.4\u9162\u9178\u30a8\u30c1\u30eb\uff1a\u6b8b\u57fa\u30b0\u30ec\u30fc\u30c9\u3002<\/p>\n<p>3.5 N-\u30d8\u30ad\u30b5\u30f3\uff1a\u6b8b\u7559\u30b0\u30ec\u30fc\u30c9\u3002<\/p>\n<p>3.6\u5869\u5316\u30ca\u30c8\u30ea\u30a6\u30e0\u6c34\u6eb6\u6db2\u3002<\/p>\n<p>3.7\u7121\u6c34\u786b\u9178\u30ca\u30c8\u30ea\u30a6\u30e0\uff1a650\u00b0C\u30674\u6642\u9593\u767a\u706b\u3057\u3001\u3057\u3063\u304b\u308a\u3068\u5bc6\u9589\u3055\u308c\u305f\u5bb9\u5668\u306b\u4fdd\u5b58\u3057\u307e\u3059\u3002<br \/>\n3.8\u5869\u5316\u30ca\u30c8\u30ea\u30a6\u30e0\u6c34\u6eb6\u6db2\uff085\uff05\uff09\uff1a\u5869\u5316\u30ca\u30c8\u30ea\u30a6\u30e05.0g\u306e\u8a08\u91cf\u3002<br \/>\n3.9 \u9162\u9178\u30a8\u30c1\u30eb-n-\u30d8\u30ad\u30b5\u30f3(1+1V\/V) 100 mL \u9162\u9178\u30a8\u30c1\u30eb\u3092250 mL\u4e09\u89d2\u30d5\u30e9\u30b9\u30b3\u306b\u5165\u308c\u3001n-\u30d8\u30ad\u30b5\u30f3100 mL\u3092\u52a0\u3048\u3066\u6df7\u305c\u307e\u3059\u3002<br \/>\n3.10\u9162\u9178\u30a8\u30c1\u30eb - \u30b7\u30af\u30ed\u30d8\u30ad\u30b5\u30f3\uff081+1V\/V): \u30dc\u30ea\u30e5\u30fc\u30e0100 ml\u306e\u9162\u9178\u30a8\u30c1\u30eb\u30a8\u30c1\u30eb\u30a8\u30c1\u30eb\u30a8\u30c1\u30eb\u30d5\u30e9\u30b9\u30b3\u306b\u306a\u308a\u3001100 mL\u306e\u30b7\u30af\u30ed\u30d8\u30ad\u30b5\u30f3\u3092\u52a0\u3048\u3066\u6df7\u305c\u307e\u3059\u3002<br \/>\n3.11\u30af\u30ed\u30eb\u30d4\u30ea\u30db\u30b9\u6a19\u6e96\uff08C9 H11C13 NO3 PS\u3001CAS No. 2921-88-2\uff09\u7d14\u5ea6&gt; 98\uff05\u3002<\/p>\n<p>3.12\u6a19\u6e96\u30b9\u30c8\u30c3\u30af\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\uff1a\u4e00\u5b9a\u91cf\u306e\u30af\u30ed\u30eb\u30d4\u30ea\u30db\u30b9\u6a19\u6e96\u3092\u6b63\u78ba\u306b\u8a08\u91cf\u3057\u3001\u5c11\u91cf\u306e\u9162\u9178\u30a8\u30c1\u30eb\u3067\u89e3\u6c7a\u3057\u307e\u3059\u3002<br \/>\n3\u300213\u6a19\u6e96\u4f5c\u696d\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\uff1a\u6a19\u6e96\u4f5c\u696d\u6eb6\u6db2\u3092\u4f5c\u308b\u305f\u3081\u306b\u3001\u9162\u9178\u30a8\u30c1\u30eb\u3067\u6a19\u6e96\u30b9\u30c8\u30c3\u30af\u6eb6\u6db2\u3092\u5fc5\u8981\u306a\u6fc3\u5ea6\u306b\u5e0c\u91c8\u3057\u307e\u3059\u3002<br \/>\n3.14 Florisil Spe\u30c1\u30e5\u30fc\u30d6\uff1aFlorisil\u3001500 mg\u30016ml\u3001\u307e\u305f\u306f\u540c\u7b49\u3002<\/p>\n<p>3\u300215\u30a2\u30af\u30c6\u30a3\u30d6\u30ab\u30fc\u30dc\u30f3SPE\u30c1\u30e5\u30fc\u30d6\uff1aenvi -carb.250 mg\u30016 ml\u3001\u307e\u305f\u306f\u540c\u7b49\u3002<br \/>\n3.16\u819c\u30d5\u30a3\u30eb\u30bf\u30fc\uff1a0\u300245um\u3002<\/p>\n<p>3\u300217\u30b0\u30e9\u30d5\u70ad\u7d20\uff1a60\u30e1\u30c3\u30b7\u30e5\u301c80\u30e1\u30c3\u30b7\u30e5<\/p>\n<p>4\u3064\u306e\u88c5\u7f6e\u3068\u6a5f\u5668<\/p>\n<p>4\u30021GC\uff1aFPD\u3092\u88c5\u5099\u3057\u305f\u30ac\u30b9\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u3001526 nm\u3002<br \/>\n4.2 GC -MS\uff1aElectro -Impact Source\uff08EL\uff09\u3092\u88c5\u5099\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>4. 3\u9060\u5fc3\u5206\u96e2\u6a5f\uff1a4000r\/min\u3002<\/p>\n<p>4.4 GPC\uff1aIsocratic\u30dd\u30f3\u30d7\u3068\u5206\u6570\u30b3\u30ec\u30af\u30bf\u30fc\u3092\u88c5\u5099\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>4\u30025\u30db\u30e2\u30b8\u30ca\u30a4\u30b6\u30fc\u3002<\/p>\n<p>4\u30026\u56de\u8ee2\u771f\u7a7a\u84b8\u767a\u5668\u3002<\/p>\n<p>4\u30027Stoppered Erlenmeyer Flask\uff1a250 ml<\/p>\n<p>4.8\u5206\u96e2\u6f0f\u6597\uff1a250 ml<\/p>\n<p>4.9\u6fc3\u7e2e\u30dc\u30c8\u30eb\uff1a50 ml\u304a\u3088\u3073250 ml<\/p>\n<p>4\u300210\u96fb\u5b50\u30d0\u30e9\u30f3\u30b9\uff1a0\u3002000 1 g\u306b\u6b63\u78ba\u3002<\/p>\n<p>4\u300211\u30d7\u30e9\u30b9\u30c1\u30c3\u30af\u9060\u5fc3\u6027\u30c1\u30e5\u30fc\u30d6\uff1a50 mL<\/p>\n<p>&nbsp;<\/p>\n<p>5\u30c6\u30b9\u30c8\u30b5\u30f3\u30d7\u30eb\u306e\u6e96\u5099\u3068\u4fdd\u5b58<br \/>\n5.1\u30c6\u30b9\u30c8\u30b5\u30f3\u30d7\u30eb\u306e\u6e96\u5099<\/p>\n<p>5.1.1\u30c8\u30a6\u30e2\u30ed\u30b3\u30b7\u3001\u8336\u8272\u306e\u30e9\u30a4\u30b9\u3001\u8336\u3001\u677e\u306e\u30ca\u30c3\u30c4\u30ab\u30fc\u30cd\u30eb\u3001\u30d4\u30fc\u30ca\u30c3\u30c4\u3001\u8702\u871c<\/p>\n<p>\u7d04500 g\u306e\u4ee3\u8868\u7684\u306a\u30b5\u30f3\u30d7\u30eb\u3092\u670d\u7528\u3057\u307e\u3059\u3002<\/p>\n<p>5.1.2\u30aa\u30ec\u30f3\u30b8\u3001\u30ea\u30f3\u30b4\u3001\u307b\u3046\u308c\u3093\u8349\u3001\u30cd\u30ae<\/p>\n<p>\u7d04500 g\u306e\u4ee3\u8868\u7684\u306a\u30b5\u30f3\u30d7\u30eb\u3092\u670d\u7528\u3057\u307e\u3059\u3002<br \/>\n5.1.3\u8c5a\u3002<\/p>\n<p>\u7d041 kg\u306e\u4ee3\u8868\u7684\u306a\u30b5\u30f3\u30d7\u30eb\u3092\u64ae\u5f71\u3057\u307e\u3059\u3002<br \/>\n5.1.4\u30c1\u30ea<\/p>\n<p>\u7d04500 g\u306e\u4ee3\u8868\u7684\u306a\u30b5\u30f3\u30d7\u30eb\u3092\u670d\u7528\u3057\u307e\u3059\u3002<br \/>\n5.2\u30c6\u30b9\u30c8\u30b5\u30f3\u30d7\u30eb\u306e\u4fdd\u5b58<\/p>\n<p>\u30b7\u30ea\u30a2\u30eb\u3001\u30ca\u30c3\u30c4\u3001\u304a\u8336\u306e\u30c6\u30b9\u30c8\u30b5\u30f3\u30d7\u30eb\u3002<br \/>\n6\u624b\u9806<\/p>\n<p>6.1\u62bd\u51fa<\/p>\n<p>6.1.1\u30aa\u30ec\u30f3\u30b8\u3001\u30ea\u30f3\u30b4\u3001\u307b\u3046\u308c\u3093\u8349\u3001\u30cd\u30ae\u3001\u30c8\u30a6\u30e2\u30ed\u30b3\u30b7\u3001\u8336\u8272\u306e\u30e9\u30a4\u30b9\u3001\u30c1\u30ea<\/p>\n<p>\u30c6\u30b9\u30c8\u30b5\u30f3\u30d7\u30eb\u306e\u91cd\u91cf\u306f50 mL\u9060\u5fc3\u30c1\u30e5\u30fc\u30d6\uff084\u300211\uff09\u306b10g\uff08\u6b63\u78ba\u306b0.01g\uff09\u3002<\/p>\n<p>6.1.2\u304a\u8336<\/p>\n<p>5g\uff08\u7cbe\u5ea60.01 g\uff09\u306e\u30c6\u30b9\u30c8\u30b5\u30f3\u30d7\u30eb\u306e50 mL\u306e\u30d7\u30e9\u30b9\u30c1\u30c3\u30af\u9060\u5fc3\u7ba1\u306b\u8a08\u91cf\u3057\u307e\u3059\u3002<br \/>\n6.1.3\u8c5a\u8089\u3001\u9d8f\u306e\u809d\u81d3\u3001\u9d8f\u306e\u814e\u81d3\u3001\u9b5a\u3001\u677e\u306e\u76ae\u8089\u3001\u30d4\u30fc\u30ca\u30c3\u30c4<\/p>\n<p>250 mL\u306e\u505c\u6b62\u3057\u305f\u30a8\u30eb\u30ec\u30f3\u30de\u30a4\u30e4\u30fc\u30d5\u30e9\u30b9\u30b3\u3078\u306e\u30c6\u30b9\u30c8\u30b5\u30f3\u30d7\u30eb\u306e\u30c6\u30b9\u30c8\u30b5\u30f3\u30d7\u30eb\u306b20 g\uff08\u7cbe\u5ea60.01g\uff09\uff08\u677e\u306e\u30cd\u30c3\u30c8\u30ab\u30fc\u30cd\u30eb\u3068\u30d4\u30fc\u30ca\u30c3\u30c4\u306e\u5834\u5408\u306f5g\uff09\u3002<br \/>\n\u62bd\u51fa\u7269\u30921\u3064\u306e250 ml\u306e\u5206\u96e2\u6f0f\u6597\u306b\u79fb\u3057\u307e\u3059\u3002<br \/>\n6.2\u30af\u30ea\u30fc\u30cb\u30f3\u30b0-UP<\/p>\n<p>6\u30022\u30021GPC\u30af\u30ea\u30fc\u30cb\u30f3\u30b0-Up<\/p>\n<p>6.2\u3002<\/p>\n<p>a\uff09GPC\u30ab\u30e9\u30e0\uff1a700 mm x 25 mm\uff08i\u3002d\u3002\uff09\u30d0\u30a4\u30aa\u30d3\u30fc\u30baS-x3\u307e\u305f\u306f\u540c\u7b49\u3002<\/p>\n<p>b\uff09\u79fb\u52d5\u76f8\uff1a\u30b7\u30af\u30ed\u30d8\u30ad\u30b5\u30f3 - \u9162\u9178\u30a8\u30c1\u30eb\uff081+1\uff09<\/p>\n<p>c\uff09\u6d41\u91cf\uff1a4\u30027ml\/min:<\/p>\n<p>d\uff09\u30b5\u30f3\u30d7\u30eb\u30eb\u30fc\u30d7\u3067\u306e\u6ce8\u5165\u91cf\uff1a10ml\uff1a<br \/>\ne\uff09\u4e8b\u524d\u30ea\u30f3\u30b7\u30f3\u30b0\u306e\u6642\u9593\uff1a10\u5206\uff1a<\/p>\n<p>f\uff09GPC\u30d0\u30e9\u30f3\u30b9\u6642\u9593\uff1a5\u5206\uff1a<\/p>\n<p>g\uff09\u6eb6\u51fa\u7269\u3092\u53ce\u96c6\u3059\u308b\u6642\u9593\uff1a22\u5206\u301c31\u5206\u3002<br \/>\na\uff09\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u30ab\u30e9\u30e0\uff1aHP 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