\u5b9f\u9a13\u76ee\u6a19
\n\u5b9f\u9a13\u306b\u3088\u308a\u3001\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u5206\u6790\u306e\u57fa\u672c\u539f\u5247\u3068\u6a5f\u5668\u306e\u57fa\u672c\u7684\u306a\u64cd\u4f5c\u3092\u5b66\u3073\u3001\u30c7\u30fc\u30bf\u5206\u6790\u306e\u30d7\u30ed\u30bb\u30b9\u3092\u7406\u89e3\u3057\u307e\u3059\u3002
\n\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u306e\u5b9a\u91cf\u7684\u65b9\u6cd5\u306b\u3064\u3044\u3066\u5b66\u3073\u3001\u3055\u307e\u3056\u307e\u306a\u65b9\u6cd5\u306e\u9577\u6240\u3068\u77ed\u6240\u3092\u6bd4\u8f03\u3057\u307e\u3059\u3002<\/p>\n
HPLC\u306f\u3058\u3081\u306b
\n\u9ad8\u901f\uff1aHPLC\u306f\u9ad8\u5727\u6ce8\u5165\u88c5\u7f6e\u3092\u4f7f\u7528\u3057\u3001\u6d41\u91cf\u306f\u5927\u5e45\u306b\u5897\u52a0\u3057\u3001\u5206\u6790\u901f\u5ea6\u306f\u975e\u5e38\u306b\u9ad8\u901f\u3067\u3001\u4e00\u822c\u7684\u306b\u6570\u5206\u3057\u304b\u3042\u308a\u307e\u305b\u3093\u3002
\n\u52b9\u7387\u7684\uff1a\u30d5\u30a3\u30e9\u30fc\u7c92\u5b50\u306f\u975e\u5e38\u306b\u7d30\u304b\u304f\u898f\u5247\u7684\u3067\u3001\u5b9a\u5e38\u76f8\u306e\u30b3\u30fc\u30c6\u30a3\u30f3\u30b0\u306f\u5747\u4e00\u3067\u3042\u308a\u3001\u7269\u8cea\u79fb\u52d5\u62b5\u6297\u306f\u5c0f\u3055\u304f\u3001\u30ab\u30e9\u30e0\u306e\u52b9\u7387\u306f\u975e\u5e38\u306b\u9ad8\u304f\u306a\u3063\u3066\u3044\u307e\u3059\u3002
\n\u9ad8\u611f\u5ea6\uff1a\u691c\u51fa\u5668\u306f\u975e\u5e38\u306b\u654f\u611f\u3067\u3059\uff1aUV-10-9g\u3001\u86cd\u5149\u691c\u51fa\u5668-10-11g\u3002<\/p>\n
GC\u3068\u306e\u6bd4\u8f03HPLC<\/p>\n
\u5206\u6790\u306e\u30aa\u30d6\u30b8\u30a7\u30af\u30c8\u3068\u7bc4\u56f2\uff1aGC\u5206\u6790\u306f\u3001\u30ac\u30b9\u3068\u4f4e\u30dc\u30fc\u30ea\u30f3\u30b0\u306e\u5b89\u5b9a\u3057\u305f\u5316\u5408\u7269\u306b\u9650\u5b9a\u3055\u308c\u3066\u304a\u308a\u3001\u3053\u308c\u306f\u5168\u6709\u6a5f\u7269\u306e20\uff05\u306e\u307f\u3092\u5360\u3081\u3066\u3044\u307e\u3059\u3002<\/p>\n
\u79fb\u52d5\u76f8\u306e\u9078\u629e\uff1aGC\u306f\u3001\u79fb\u52d5\u76f8\u3068\u3057\u3066\u9650\u5b9a\u7684\u306a\u30aa\u30d7\u30b7\u30e7\u30f3\u306e\u7a2e\u985e\u306e\u300c\u4e0d\u6d3b\u6027\u300d\u30ac\u30b9\u3092\u4f7f\u7528\u3057\u3001\u30b3\u30f3\u30dd\u30fc\u30cd\u30f3\u30c8\u306b\u308f\u305a\u304b\u306a\u5f71\u97ff\u3092\u53ca\u307c\u3057\u307e\u3059\u3002<\/p>\n
\u52d5\u4f5c\u6e29\u5ea6\uff1aGC\u306b\u306f\u9ad8\u6e29\u304c\u5fc5\u8981\u3067\u3059\u3002
\n\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u30d5\u30a3\u30fc\u30eb\u30c9
\n\u9ad8\u611f\u5ea6\u3001\u5b9a\u91cf\u7684\u7cbe\u5ea6\u3001\u304a\u3088\u3073\u975e\u63ee\u767a\u6027\u304a\u3088\u3073\u71b1\u7684\u306b\u4e0d\u5b89\u5b9a\u306a\u6210\u5206\u306e\u5206\u6790\u306b\u9069\u3057\u3066\u3044\u308b\u305f\u3081\u3001HPLC\u5206\u96e2\u5206\u6790\u306f\u3001\u7523\u696d\u304a\u3088\u3073\u79d1\u5b66\u7684\u7814\u7a76\u3001\u7279\u306b\u751f\u7269\u5b66\u3068\u533b\u5b66\u306b\u304a\u3044\u3066\u975e\u5e38\u306b\u7528\u9014\u304c\u5e83\u3044\u3067\u3059\u3002<\/p>\n
HPLC\u306f\u3001\u30dd\u30f3\u30d7\u30b7\u30b9\u30c6\u30e0\u3001\u6ce8\u5165\u30b7\u30b9\u30c6\u30e0\u3001\u5206\u96e2\u30b7\u30b9\u30c6\u30e0\u3001\u691c\u51fa\u5668\u3001\u304a\u3088\u3073\u30c7\u30fc\u30bf\u51e6\u7406\u30b7\u30b9\u30c6\u30e0\u3067\u69cb\u6210\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n
\u9ad8\u5727\u6ce8\u5165\u30b7\u30b9\u30c6\u30e0
\n1\uff09\u79fb\u52d5\u76f8\u30dc\u30c8\u30eb\uff1a\u6eb6\u5a92\u30d5\u30a3\u30eb\u30bf\u30fc\uff08Ni\u5408\u91d1\uff09\u3092\u5099\u3048\u305f1-2L\u30ac\u30e9\u30b9\u30dc\u30c8\u30eb\u3001\u305d\u306e\u5b54\u306f\u7d042\u03bcm\u3067\u3042\u308a\u3001\u5fae\u7c92\u5b50\u304c\u6c72\u307f\u4e0a\u3052\u3089\u308c\u306a\u3044\u3088\u3046\u306b\u3057\u307e\u3059\u3002
\n2\uff09\u8131\u30ac\u30c3\u30b5\u30fc\uff1a\u8d85\u97f3\u6ce2\u306e\u8131\u30ac\u30b9\u307e\u305f\u306f\u771f\u7a7a\u52a0\u71b1\u306e\u8131\u30ac\u30b9\u3002<\/p>\n
3\uff09\u9ad8\u5727\u30dd\u30f3\u30d7\uff1a\u6ce8\u5165\u30dd\u30f3\u30d7\u306e\u8981\u4ef6\uff1a\u826f\u597d\u306a\u30b7\u30fc\u30ea\u30f3\u30b0\u3001\u6d41\u5165\u306e\u306a\u3044\u5b89\u5b9a\u3057\u305f\u6d41\u308c\u3001\u5e45\u5e83\u3044\u8abf\u6574\u53ef\u80fd\u306a\u7bc4\u56f2\u3001\u8010\u98df\u6027\u3002<\/p>\n
\u5b9f\u9a13<\/p>\n
\u5b9f\u9a13\u6761\u4ef6
\n1\uff09\u30e1\u30bf\u30ce\u30fc\u30eb\u306e\u3055\u307e\u3056\u307e\u306a\u6bd4\u7387\u306e\u643a\u5e2f\u30d5\u30a7\u30fc\u30ba\u306710\u03bcL\u6df7\u5408\u6a19\u6e96\u6eb6\u6db2\uff08\u30e1\u30c1\u30eb\u30d1\u30e9\u30d9\u30f3\u3001\u30d7\u30ed\u30d4\u30eb\u30d1\u30e9\u30d9\u30f3\u3001\u304a\u3088\u3073\u30d6\u30c1\u30eb\u30d1\u30e9\u30d9\u30f3\uff09\u3092\u6ce8\u5165\u3057\u307e\u3059\u3002
\n\u6ce8\uff1a\u79fb\u52d5\u76f8\u3092\u5909\u66f4\u3059\u308b\u305f\u3073\u306b\u3001\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u30b7\u30b9\u30c6\u30e0\u306e\u30d0\u30e9\u30f3\u30b9\u3092\u3068\u308b\u305f\u3081\u306b\u7d045\u5206\u9593\u5f85\u3064\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\uff08\u4fe1\u53f7\u30e9\u30a4\u30f3\u3068\u5727\u529b\u30e9\u30a4\u30f3\u306e\u4e21\u65b9\u304c\u30d0\u30e9\u30f3\u30b9\u304c\u53d6\u308c\u3066\u3044\u307e\u3059\uff09\u3002
\n2\uff090.8\u30011.0\u3001\u304a\u3088\u30731.5 mL {{url_placeholder_0}}\u306e\u7570\u306a\u308b\u79fb\u52d5\u76f8\u6d41\u91cf\u306b10\u03bcl\u306e\u6df7\u5408\u6a19\u6e96\u6eb6\u6db2\uff08\u30e1\u30c1\u30eb\u30d1\u30e9\u30d9\u30f3\u3001\u30d7\u30ed\u30d4\u30eb\u30d1\u30e9\u30d9\u30f3\u3001\u304a\u3088\u3073\u30d6\u30c1\u30eb\u30d1\u30e9\u30d9\u30f3\uff09\u3092\u6ce8\u5165\u3057\u3001\u6700\u9069\u306a\u79fb\u52d5\u76f8\u5674\u5c04\u6ce8\u5c04\u5206\u6790\u3067\u3001\u540c\u3058\u539f\u5247\u304c\u6700\u826f\u306e\u79fb\u52d5\u76f8\u6d41\u91cf\u3092\u9078\u629e\u3057\u307e\u3057\u305f\u3002<\/p>\n
\u5b9a\u6027\u5206\u6790
\n\u6700\u9069\u306a\u5b9f\u9a13\u6761\u4ef6\u4e0b\u3067\u306f\u3001\u30e1\u30c1\u30eb\u30d1\u30e9\u30d9\u30f3\u3001\u30d7\u30ed\u30d4\u30eb\u30d1\u30e9\u30d9\u30f3\u3001\u30d6\u30c1\u30eb\u30d1\u30e9\u30d9\u30f3\u306e\u305d\u308c\u305e\u308c10\u03bcl\u3092\u6ce8\u5165\u3057\u3001\u5404\u30d4\u30fc\u30af\u306e\u4fdd\u6301\u6642\u9593\u3092\u8a18\u9332\u3057\u307e\u3059\u3002<\/p>\n
\u5b9a\u91cf\u5206\u6790
\n\u30ad\u30e3\u30ea\u30d6\u30ec\u30fc\u30b7\u30e7\u30f3\u66f2\u7dda
\n\u6700\u9069\u306a\u5b9f\u9a13\u6761\u4ef6\u4e0b\u3067\u306f\u30012\u30015\u30018\u300110\u300115\u3001\u304a\u3088\u307320\u03bcL\u306e\u6df7\u5408\u6a19\u6e96\u30b5\u30f3\u30d7\u30eb\u3092\u6ce8\u5165\u3057\u3066\u5206\u6790\u3057\u3001\u6a19\u6e96\u66f2\u7dda\u306f\u6ce8\u5165\u5bb9\u7a4d\u3068\u30d4\u30fc\u30af\u9762\u7a4d\u306b\u3088\u3063\u3066\u78ba\u7acb\u3055\u308c\u307e\u3059\u3002
\n\u3053\u306e\u66f2\u7dda\u306e\u76f8\u95a2\u4fc2\u6570\u306f0.99\u3092\u8d85\u3048\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n
\u672a\u77e5\u306e\u30b5\u30f3\u30d7\u30eb\u5206\u6790
\n10\u03bcL\u672a\u77e5\u306e\u30b5\u30f3\u30d7\u30eb\u3092\u30c6\u30b9\u30c8\u3059\u308b\u305f\u3081\u306b\u6ce8\u5165\u3057\u3001\u30ad\u30e3\u30ea\u30d6\u30ec\u30fc\u30b7\u30e7\u30f3\u66f2\u7dda\u306b\u3088\u308b\u5b9a\u91cf\u5206\u6790\u3002<\/p>\n
\u8cea\u554f
\n\u5b9f\u9a13\u30c7\u30fc\u30bf\u306b\u57fa\u3065\u3044\u3066\u3001\u30ac\u30b9\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u3068\u6db2\u4f53\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u306e\u9593\u306e\u5206\u6790\u65b9\u6cd5\u306e\u9055\u3044\u306b\u3064\u3044\u3066\u8aac\u660e\u3057\u307e\u3059\u3002
\nHPLC\u5206\u6790\u306e\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u5024\u3092\u8abf\u3079\u307e\u3059\u3002
\nUV\u691c\u51fa\u5668\u304c\u3059\u3079\u3066\u306e\u6709\u6a5f\u5316\u5408\u7269\u3092\u691c\u51fa\u3059\u308b\u306e\u306b\u9069\u3057\u3066\u3044\u308b\u304b\u3069\u3046\u304b\uff1f<\/p>","protected":false},"excerpt":{"rendered":"
Experiment Objectives By experiments, learn the basic principles of chromatographic analysis and the basic operation of the instrument, and understand the process of data analysis. Learn about chromatographic quantitative methods and compare the advantages and disadvantages of different methods. HPLC Introduction High speed: HPLC uses high-pressure infusion equipment, the flow rate is greatly increased, and the analysis speed is extremely fast, only a few minutes generally; while the classical method depends on gravity feeding, it takes several hours to complete an analysis. Efficient: The filler particles are very fine and regular, the coating on the stationary phase is uniform, and the mass transfer resistance is small, so the column efficiency […]<\/p>\n","protected":false},"author":1,"featured_media":3315,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[76],"tags":[],"class_list":["post-2720","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-education"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.pgeneral.com\/ja\/wp-json\/wp\/v2\/posts\/2720","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.pgeneral.com\/ja\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.pgeneral.com\/ja\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/ja\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/ja\/wp-json\/wp\/v2\/comments?post=2720"}],"version-history":[{"count":0,"href":"https:\/\/www.pgeneral.com\/ja\/wp-json\/wp\/v2\/posts\/2720\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/ja\/wp-json\/wp\/v2\/media\/3315"}],"wp:attachment":[{"href":"https:\/\/www.pgeneral.com\/ja\/wp-json\/wp\/v2\/media?parent=2720"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.pgeneral.com\/ja\/wp-json\/wp\/v2\/categories?post=2720"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.pgeneral.com\/ja\/wp-json\/wp\/v2\/tags?post=2720"}],"curies":[{"name":"WP","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}