{"id":3905,"date":"2025-09-04T11:10:27","date_gmt":"2025-09-04T03:10:27","guid":{"rendered":"https:\/\/www.pgeneral.com\/?p=3905"},"modified":"2025-09-04T11:10:27","modified_gmt":"2025-09-04T03:10:27","slug":"determination-of-lead-in-food-additive-sodium-pyrophosphate-flame-atomic-absorption-spectrometry","status":"publish","type":"post","link":"https:\/\/www.pgeneral.com\/id\/applications\/determination-of-lead-in-food-additive-sodium-pyrophosphate-flame-atomic-absorption-spectrometry","title":{"rendered":"Penentuan Timbal dalam Aditif Makanan Natrium Pirofosfat (Spektrometri Penyerapan Atom Api)"},"content":{"rendered":"

1. Gambaran Umum Metode<\/h2>\n
Setelah sampel diobati, ion timah membentuk kompleks dengan natrium diethyldithiocarbamate (DDTC) di bawah kondisi pH tertentu. Kompleks diekstraksi dan dipisahkan dengan 4-metil-2-pentanone (MIBK), kemudian diperkenalkan ke dalam spektrometer penyerapan atom. Setelah atomisasi api, absorbansi diukur pada panjang gelombang 283,3 nm. Dalam kisaran konsentrasi tertentu, nilai penyerapan timah proporsional dengan kandungan, dan kuantifikasi dilakukan dengan perbandingan dengan seri standar.<\/div>\n
\n

2. Instrumen dan Reagen<\/h2>\n

2.1 Instrumen dan Peralatan<\/h3>\n

2.1.1 Instrumen Pengujian<\/h4>\n
\n
\n\n\n\n\n\n\n\n
Serial No.<\/th>\nNama<\/th>\nKuantitas<\/th>\nPersyaratan Teknis<\/th>\nAksesoris<\/th>\n<\/tr>\n<\/thead>\n
1<\/td>\nSpektrofotometer Penyerapan Atom Api<\/td>\n1 set<\/td>\n–<\/td>\nTimbal Hollow Cathode Lamp<\/td>\n<\/tr>\n
2<\/td>\nKompresor Udara<\/td>\n1 set<\/td>\nTekanan pelepasan nominal: 0,3 MPa<\/td>\n–<\/td>\n<\/tr>\n
3<\/td>\nGas asetilena<\/td>\n1 silinder<\/td>\nKemurnian \u2265 99,99%<\/td>\n–<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n

2.1.2 Sampel Peralatan Praperawatan<\/h4>\n
\n
\n\n\n\n\n\n\n\n\n\n\n
Serial No.<\/th>\nNama<\/th>\nKuantitas<\/th>\nPersyaratan Teknis<\/th>\nAksesoris<\/th>\n<\/tr>\n<\/thead>\n
1<\/td>\nKeseimbangan Elektronik<\/td>\n1 set<\/td>\nSensitivitas: 0,1 mg<\/td>\n–<\/td>\n<\/tr>\n
2<\/td>\ngelas<\/td>\nBeberapa<\/td>\nVolume: 150 ml<\/td>\n–<\/td>\n<\/tr>\n
3<\/td>\nHot Plate yang dapat disesuaikan<\/td>\n1 set<\/td>\nKisaran suhu: Suhu kamar ~ 300 \u2103<\/td>\n–<\/td>\n<\/tr>\n
4<\/td>\nTerbang pemisah<\/td>\nBeberapa<\/td>\nVolume: 250 ml<\/td>\n–<\/td>\n<\/tr>\n
5<\/td>\nMikropipet<\/td>\n1 masing-masing<\/td>\nKisaran: 100 \u03bcL ~ 1000 \u03bcL, 1000 \u03bcL ~ 5000 \u03bcL<\/td>\n–<\/td>\n<\/tr>\n
6<\/td>\nTabung Kolorimetrik<\/td>\nBeberapa<\/td>\nVolume: 10 ml<\/td>\n–<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n

2.2 Reagen<\/h3>\n

2.2.1 Reagen mentah<\/h4>\n
\n
\n\n\n\n\n\n\n\n\n\n\n\n
Serial No.<\/th>\nNama<\/th>\nPersyaratan Teknis<\/th>\nPerkataan<\/th>\n<\/tr>\n<\/thead>\n
1<\/td>\n4-Metil-2-pentanone (MIBK)<\/td>\nReagen Analisis (AR)<\/td>\n–<\/td>\n<\/tr>\n
2<\/td>\nAir Amonia<\/td>\nReagen Analisis (AR)<\/td>\n–<\/td>\n<\/tr>\n
3<\/td>\nAmmonium sulfat<\/td>\nReagen yang dijamin (GR)<\/td>\n–<\/td>\n<\/tr>\n
4<\/td>\nAmmonium Sitrat<\/td>\nReagen Analisis (AR)<\/td>\n–<\/td>\n<\/tr>\n
5<\/td>\nNatrium Diethyldithiocarbamate (DDTC)<\/td>\nReagen yang dijamin (GR)<\/td>\n–<\/td>\n<\/tr>\n
6<\/td>\nBromothymol Biru<\/td>\nReagen Analisis (AR)<\/td>\n–<\/td>\n<\/tr>\n
7<\/td>\nAsam Nitrat<\/td>\nKelas MOS<\/td>\n–<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n

2.2 Reagen yang disiapkan<\/h4>\n
\n
\n\n\n\n\n\n\n\n\n\n\n
Serial No.<\/th>\nNama<\/th>\nMetode Persiapan<\/th>\nPerkataan<\/th>\n<\/tr>\n<\/thead>\n
1<\/td>\nSolusi Air Amonia (1 1)<\/td>\nUkur 50 ml air amonia, tambahkan ke 50 ml air, dan campur dengan baik.<\/td>\n–<\/td>\n<\/tr>\n
2<\/td>\nSolusi amonium sulfat (300 g \/ L)<\/td>\nTingkatkan 30 g amonium sulfat, larutkan dalam air, dan cairkan hingga 100 mL dengan air.<\/td>\n–<\/td>\n<\/tr>\n
3<\/td>\nSolusi Amonium Citrate (250 g \/ L)<\/td>\nTimbangkan 25 g amonium sitrat, larutkan dalam air, dan cairkan hingga 100 mL dengan air.<\/td>\n–<\/td>\n<\/tr>\n
4<\/td>\nSolusi Natrium Diethyldithiocarbamate (DDTC) (50 g \/ L)<\/td>\nTimbangkan 5 g natrium diethyldithiocarbamate, larutkan dalam air, dan cairkan hingga 100 mL dengan air.<\/td>\n–<\/td>\n<\/tr>\n
5<\/td>\nSolusi Indikator Biru Bromothymol (1 g \/ L)<\/td>\nBerat 0,1 g bromothymol biru, larutkan dalam air, dan cairkan hingga 100 mL dengan air, lalu campur dengan baik.<\/td>\n–<\/td>\n<\/tr>\n
6<\/td>\nSolusi Asam Nitrat (5 95)<\/td>\nUkur 50 mL asam nitrat, tambahkan ke 950 mL air, dan campur dengan baik.<\/td>\n–<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n

2.3 Standar referensi<\/h3>\n

2.3.1 Solusi stok<\/h4>\n
\n
\n\n\n\n\n\n
Serial No.<\/th>\nTidak, tidak.<\/th>\nNama<\/th>\nPersyaratan Teknis<\/th>\nPerkataan<\/th>\n<\/tr>\n<\/thead>\n
1<\/td>\nGSB G 62071-90<\/td>\nSolusi Standar Timbal<\/td>\nKonsentrasi: 1000 \u03bcg \/ ml<\/td>\nInstitut Penelitian Besi dan Baja Pusat, Pusat Pengujian Bahan Besi dan Baja Nasional<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n

3. Perhitungan Hasil<\/h2>\n
Kandungan timbal diekspresikan sebagai fraksi massa\u00a0W<\/span>\u00a0dari timbal (Pb), dalam satuan mg\/kg, dihitung dengan rumus berikut:<\/div>\n
\"\"<\/div>\n
Di mana:<\/div>\n
    \n
  • M1<\/span>= Massa timbal dalam larutan uji yang diperoleh dari kurva kerja, unit: miligram (mg);<\/li>\n
  • M0<\/span>\u00a0= Massa timbal dalam larutan uji kosong yang diperoleh dari kurva kerja, unit: miligram (mg);<\/li>\n
  • M<\/span>\u00a0= Massa sampel, unit: gram (g).<\/li>\n<\/ul>\n

     <\/p>\n

    Rata-rata aritmatika dari hasil penentuan paralel diambil sebagai hasil penentuan akhir. Perbedaan mutlak antara dua hasil penentuan paralel tidak boleh melebihi 1 mg\/kg.<\/div>\n<\/div>","protected":false},"excerpt":{"rendered":"

    1. Method Overview After the sample is treated, lead ions form a complex with sodium diethyldithiocarbamate (DDTC) under a certain pH condition. The complex is extracted and separated with 4-methyl-2-pentanone (MIBK), then introduced into an atomic absorption spectrometer. After flame atomization, the absorbance is measured at a wavelength of 283.3 nm. Within a certain concentration […]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[36,73],"tags":[],"class_list":["post-3905","post","type-post","status-publish","format-standard","hentry","category-applications","category-food-and-drugs"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.pgeneral.com\/id\/wp-json\/wp\/v2\/posts\/3905","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.pgeneral.com\/id\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.pgeneral.com\/id\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/id\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/id\/wp-json\/wp\/v2\/comments?post=3905"}],"version-history":[{"count":0,"href":"https:\/\/www.pgeneral.com\/id\/wp-json\/wp\/v2\/posts\/3905\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.pgeneral.com\/id\/wp-json\/wp\/v2\/media?parent=3905"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.pgeneral.com\/id\/wp-json\/wp\/v2\/categories?post=3905"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.pgeneral.com\/id\/wp-json\/wp\/v2\/tags?post=3905"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}