{"id":3790,"date":"2025-07-28T16:03:51","date_gmt":"2025-07-28T08:03:51","guid":{"rendered":"https:\/\/www.pgeneral.com\/?p=3790"},"modified":"2025-07-28T16:03:51","modified_gmt":"2025-07-28T08:03:51","slug":"determination-of-lead-in-drinking-water-graphite-furnace-atomic-absorption-spectrometry","status":"publish","type":"post","link":"https:\/\/www.pgeneral.com\/id\/applications\/determination-of-lead-in-drinking-water-graphite-furnace-atomic-absorption-spectrometry","title":{"rendered":"Penentuan Timbal dalam Air Minum (Spektrometri Penyerapan Atom Tungku Grafit)"},"content":{"rendered":"

1. Gambaran Umum Metode<\/h2>\n
Setelah perawatan yang tepat, sampel diatomisasi dalam tungku grafit, dan absorbansi diukur pada 283,3 nm. Dalam kisaran konsentrasi tertentu, penyerapan timah proporsional dengan kandungan, dan kuantifikasi dilakukan dengan perbandingan dengan seri standar.<\/div>\n
\n

2. Instrumen dan Reagen<\/h2>\n

2.1 Instrumen dan Peralatan<\/h3>\n

2.1.1 Instrumen Pengujian<\/h4>\n
\n\n\n\n\n\n\n
Nama<\/th>\nKuantitas<\/th>\nPersyaratan Teknis<\/th>\nAksesoris<\/th>\n<\/tr>\n<\/thead>\n
Mikropipet<\/td>\n1 masing-masing<\/td>\n100 \u03bcL ~ 1000 \u03bcL, 1000 \u03bcL ~ 5000 \u03bcL, 5 ~ 50 \u03bcL<\/td>\n<\/td>\n<\/tr>\n
Kolos volumetrik<\/td>\nBeberapa<\/td>\n100 ml<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

2.1.2 Peralatan Praperawatan<\/h4>\n
\n\n\n\n\n\n\n
Serial No.<\/th>\nNama<\/th>\nKuantitas<\/th>\nPersyaratan Teknis<\/th>\nAksesoris<\/th>\n<\/tr>\n<\/thead>\n
1<\/td>\nMikropipet<\/td>\n1 masing-masing<\/td>\n100 \u03bcL ~ 1000 \u03bcL, 1000 \u03bcL ~ 5000 \u03bcL, 5 ~ 50 \u03bcL<\/td>\n<\/td>\n<\/tr>\n
2<\/td>\nKolos volumetrik<\/td>\nBeberapa<\/td>\n100 ml<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

2.2 Reagen<\/h3>\n

2.2.1 Reagen<\/h4>\n
\n\n\n\n\n\n
Serial No.<\/th>\nNama<\/th>\nPersyaratan Teknis<\/th>\nPerkataan<\/th>\n<\/tr>\n<\/thead>\n
1<\/td>\nAsam nitrat<\/td>\nkelas MOS<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

2.3 Standar referensi<\/h3>\n

2.3.1 Solusi Saham<\/h4>\n
\n\n\n\n\n\n
Serial No.<\/th>\nTidak, tidak.<\/th>\nNama<\/th>\nPersyaratan Teknis<\/th>\nPerkataan<\/th>\n<\/tr>\n<\/thead>\n
1<\/td>\nAngka 08619<\/td>\nBahan standar larutan elemen tunggal timbal<\/td>\n1000 \u03bcg \/ ml<\/td>\nInstitut Metrologi Nasional, Cina<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

3. Prosedur Operasional<\/h2>\n

3.1 Pengolahan Sampel<\/h3>\n

3.1.1 Persiapan Solusi Uji<\/h4>\n
Ambil 100 mL sampel air ke dalam botol volumetrik, tambahkan 0,5 mL asam nitrat, dan campur dengan baik. Lakukan tes kosong di samping sampel.<\/div>\n

3.1.2 Persiapan Solusi Standar<\/h4>\n
    \n
  1. \n
    Persiapan larutan menengah standar timah: Larutan standar timah (1,0 \u03bcg \/ mL): Pipet akurat 0,1 mL larutan standar timah (1000 \u03bcg \/ mL) ke dalam kolb volumetrik 100 mL, tambahkan 0,5 mL larutan asam nitrat, cairkan ke tanda, dan goyang dengan baik.<\/div>\n<\/li>\n
  2. \n
    Persiapan seri standar timah: Pipet 0,0, 0,1, 0,2, 0,3, dan 0,4 mL larutan standar timah (1,0 \u03bcg \/ mL) secara akurat ke dalam botol volumetrik 10 mL masing-masing. Tambahkan 0,5 mL larutan asam nitrat ke masing-masing, cairkan ke tanda, dan mendapatkan larutan seri standar timah dengan konsentrasi 0,0, 10,0, 20,0, 30,0, dan 40,0 ng \/ mL.<\/div>\n<\/li>\n<\/ol>\n

    3.2 Pengujian Sampel<\/h3>\n
      \n
    1. Kondisi Pengujian Kondisi referensi untuk deteksi spektrofotometer penyerapan atom tungku grafit<\/li>\n<\/ol>\n
      \n\n\n\n\n\n\n\n\n
      Elemen<\/th>\nMemimpin<\/th>\n<\/tr>\n<\/thead>\n
      Panjang gelombang (nm)<\/td>\n283.3<\/td>\n<\/tr>\n
      Lebar pita spektral (nm)<\/td>\n0.4<\/td>\n<\/tr>\n
      Arus lampu elemen (mA)<\/td>\n2.0<\/td>\n<\/tr>\n
      Metode koreksi latar belakang<\/td>\nLampu Deuterium<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

       <\/p>\n<\/div>\n

      Program Pemanasan Tungku Grafit<\/strong><\/div>\n

       <\/p>\n

      \n\n\n\n\n\n\n\n\n
      Langkah<\/th>\nProsedur<\/th>\nsuhu (\u2103)<\/th>\nWaktu Ramp (s)<\/th>\nTahan Waktu (s)<\/th>\n<\/tr>\n<\/thead>\n
      1<\/td>\nPengeringan<\/td>\n120<\/td>\n10<\/td>\n10<\/td>\n<\/tr>\n
      2<\/td>\nAshing<\/td>\n450<\/td>\n10<\/td>\n10<\/td>\n<\/tr>\n
      3<\/td>\nAtomisi<\/td>\n1800<\/td>\n0<\/td>\n2<\/td>\n<\/tr>\n
      4<\/td>\nPembersihan<\/td>\n1900<\/td>\n1<\/td>\n2<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

       <\/p>\n

        \n
      1. Pengujian Sampel Tentukan penyerapan solusi standar, solusi uji kosong, dan solusi uji secara berurutan. Kurangkan penyerapan larutan standar nol dari penyerapan setiap larutan standar. Plot kurva kerja dengan konsentrasi massa sebagai absisa dan absorbansi yang sesuai sebagai ordinat. Berdasarkan penyerapan yang diukur dari larutan uji, temukan konsentrasi massa timbal dari kurva kerja.<\/li>\n<\/ol>\n

        3.3 Perhitungan Hasil<\/h3>\n
        Rumus:<\/div>\n

        \"\"<\/p>\n

        Di mana:<\/div>\n
        \u03c1 - Konsentrasi massa timah dalam sampel air, dalam miligram per liter (mg \/ L);<\/div>\n
        \u03c11<\/span>Konsentrasi massa timbal dalam sampel air yang diperoleh dari kurva standar, dalam nanogram per mililiter (ng \/ mL);<\/div>\n
        V1<\/span>\u00a0Volume larutan sampel untuk penentuan, dalam mililiter (mL);<\/div>\n
        dalam<\/span>\u00a0- Volume sampel air asli, dalam mililiter (mL);<\/div>\n
        1000 Faktor konversi.<\/div>\n<\/div>\n<\/div>","protected":false},"excerpt":{"rendered":"

        1. Method Overview After appropriate treatment, the sample is atomized in a graphite furnace, and the absorbance is measured at 283.3 nm. Within a certain concentration range, the absorbance of lead is proportional to its content, and quantification is performed by comparison with a standard series. 2. Instruments and Reagents 2.1 Instruments and Equipment 2.1.1 […]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[36,73],"tags":[],"class_list":["post-3790","post","type-post","status-publish","format-standard","hentry","category-applications","category-food-and-drugs"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.pgeneral.com\/id\/wp-json\/wp\/v2\/posts\/3790","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.pgeneral.com\/id\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.pgeneral.com\/id\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/id\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/id\/wp-json\/wp\/v2\/comments?post=3790"}],"version-history":[{"count":0,"href":"https:\/\/www.pgeneral.com\/id\/wp-json\/wp\/v2\/posts\/3790\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.pgeneral.com\/id\/wp-json\/wp\/v2\/media?parent=3790"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.pgeneral.com\/id\/wp-json\/wp\/v2\/categories?post=3790"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.pgeneral.com\/id\/wp-json\/wp\/v2\/tags?post=3790"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}