{"id":4483,"date":"2026-03-16T10:36:00","date_gmt":"2026-03-16T02:36:00","guid":{"rendered":"https:\/\/www.pgeneral.com\/?p=4483"},"modified":"2026-03-16T10:36:00","modified_gmt":"2026-03-16T02:36:00","slug":"determination-of-vitamin-b%e2%82%82ultraviolet-visible-spectrophotometry-2","status":"publish","type":"post","link":"https:\/\/www.pgeneral.com\/fr\/applications\/bio-tech-and-life-sciences\/determination-of-vitamin-b%e2%82%82ultraviolet-visible-spectrophotometry-2","title":{"rendered":"D\u00e9termination de la vitamine B \u2082  (Spectrophotom\u00e9trie visible ultraviolette)"},"content":{"rendered":"<ol>\n<li>Aper\u00e7u de la m\u00e9thode<\/li>\n<\/ol>\n<p>Cette m\u00e9thode est bas\u00e9e sur la norme nationale de s\u00e9curit\u00e9 alimentaire GB 12752-2010 &#8211; Additif alimentaire Vitamine B \u2082  (Riboflavine). Apr\u00e8s dissoudre la vitamine B \u2082, il est dilu\u00e9 avec une solution d'ac\u00e9tate de sodium, et une cuvette de quartz de 1cm est utilis\u00e9e pour le balayage spectral pour d\u00e9terminer le pic d'absorption maximum. Le rapport d'absorbance \u00e0 375nm \u00e0 celle \u00e0 267nm devrait \u00eatre de 0,31 \u00e0 0,33, et le rapport d'absorbance \u00e0 222nm \u00e0 celle \u00e0 267nm devrait \u00eatre de 0,36 \u00e0 0,39.<\/p>\n<ol start=\"2\">\n<li>Port\u00e9e<\/li>\n<\/ol>\n<p>vitamine B2<\/p>\n<ol start=\"3\">\n<li>R\u00e9f\u00e9rences<\/li>\n<\/ol>\n<p>GB 14752-2010 *Norme nationale de s\u00e9curit\u00e9 alimentaire &#8211; Additif alimentaire Vitamine B2 (riboflavine)*<\/p>\n<p>4. Instruments d &apos; essai<\/p>\n<p>Spectrophotom\u00e8tre UV TU1901 (240nm ~ 465nm)<\/p>\n<p>Cuvette de quartz 1cm<\/p>","protected":false},"excerpt":{"rendered":"<p>Method Overview This method is based on GB 12752-2010 National Food Safety Standard &#8211; Food Additive Vitamin B\u2082(Riboflavin). After dissolving vitamin B\u2082, it is diluted with sodium acetate solution, and a 1cm quartz cuvette is used for spectral scanning to determine the maximum absorption peak. The absorbance ratio at 375nm to that at 267nm should [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[77],"tags":[],"class_list":["post-4483","post","type-post","status-publish","format-standard","hentry","category-bio-tech-and-life-sciences"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.pgeneral.com\/fr\/wp-json\/wp\/v2\/posts\/4483","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.pgeneral.com\/fr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.pgeneral.com\/fr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/fr\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/fr\/wp-json\/wp\/v2\/comments?post=4483"}],"version-history":[{"count":0,"href":"https:\/\/www.pgeneral.com\/fr\/wp-json\/wp\/v2\/posts\/4483\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.pgeneral.com\/fr\/wp-json\/wp\/v2\/media?parent=4483"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.pgeneral.com\/fr\/wp-json\/wp\/v2\/categories?post=4483"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.pgeneral.com\/fr\/wp-json\/wp\/v2\/tags?post=4483"}],"curies":[{"name":"WP","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}