{"id":4483,"date":"2026-03-16T10:36:00","date_gmt":"2026-03-16T02:36:00","guid":{"rendered":"https:\/\/www.pgeneral.com\/?p=4483"},"modified":"2026-03-16T10:36:00","modified_gmt":"2026-03-16T02:36:00","slug":"determination-of-vitamin-b%e2%82%82ultraviolet-visible-spectrophotometry-2","status":"publish","type":"post","link":"https:\/\/www.pgeneral.com\/es\/applications\/bio-tech-and-life-sciences\/determination-of-vitamin-b%e2%82%82ultraviolet-visible-spectrophotometry-2","title":{"rendered":"Determinaci\u00f3n de la vitamina B \u2082  (Espectrofotometr\u00eda visible ultravioleta)"},"content":{"rendered":"<ol>\n<li>Descripci\u00f3n general del m\u00e9todo<\/li>\n<\/ol>\n<p>Este m\u00e9todo se basa en la norma nacional de seguridad alimentaria GB 12752-2010 &#8211; Aditivo alimentario Vitamina B \u2082  (Riboflavina). Despu\u00e9s de disolver la vitamina B \u2082, se diluye con soluci\u00f3n de acetato de sodio, y se utiliza una cubeta de cuarzo de 1 cm para el escaneo espectral para determinar el pico de absorci\u00f3n m\u00e1ximo. La relaci\u00f3n de absorbancia a 375 nm a la de 267 nm debe ser de 0,31 a 0,33, y la relaci\u00f3n de absorbancia a 222 nm a la de 267 nm debe ser de 0,36 a 0,39.<\/p>\n<ol start=\"2\">\n<li>\u00c1mbito de aplicaci\u00f3n<\/li>\n<\/ol>\n<p>vitamina B2<\/p>\n<ol start=\"3\">\n<li>Referencias<\/li>\n<\/ol>\n<p>GB 14752-2010 * Est\u00e1ndar Nacional de Seguridad Alimentaria &#8211; Aditivo alimentario Vitamina B2 (riboflavina)*<\/p>\n<p>4. Instrumentos de prueba<\/p>\n<p>Espectrofot\u00f3metro UV TU1901 (240nm ~ 465nm)<\/p>\n<p>Cueta de cuarzo de 1 cm<\/p>","protected":false},"excerpt":{"rendered":"<p>Method Overview This method is based on GB 12752-2010 National Food Safety Standard &#8211; Food Additive Vitamin B\u2082(Riboflavin). After dissolving vitamin B\u2082, it is diluted with sodium acetate solution, and a 1cm quartz cuvette is used for spectral scanning to determine the maximum absorption peak. The absorbance ratio at 375nm to that at 267nm should [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[77],"tags":[],"class_list":["post-4483","post","type-post","status-publish","format-standard","hentry","category-bio-tech-and-life-sciences"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.pgeneral.com\/es\/wp-json\/wp\/v2\/posts\/4483","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.pgeneral.com\/es\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.pgeneral.com\/es\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/es\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.pgeneral.com\/es\/wp-json\/wp\/v2\/comments?post=4483"}],"version-history":[{"count":0,"href":"https:\/\/www.pgeneral.com\/es\/wp-json\/wp\/v2\/posts\/4483\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.pgeneral.com\/es\/wp-json\/wp\/v2\/media?parent=4483"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.pgeneral.com\/es\/wp-json\/wp\/v2\/categories?post=4483"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.pgeneral.com\/es\/wp-json\/wp\/v2\/tags?post=4483"}],"curies":[{"name":"WP","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}